MitoFit Science Camp 2016 Kuehtai AT

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MitoFit Science Camp 2016 Kuehtai AT

Kuehtai AT, 2016 Jul 07-13. MitoFit Science Camp and IOC112.

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Oroboros (2016-07-07) MitoGlobal

Abstract: 2016 Jul 07-13, Kuehtai, AT. The project MitoFit aims at developing novel laboratory standards and diagnostic monitoring of a mitochondrial fitness score. With an international team of outstanding mitochondrial experts, the MitoFit Science Camp will provide a unique opportunity to receive first-hand introductions to state-of-the-art diagnostic monitoring of mitochondrial respiratory function combined with hands-on training on HRR (IOC112). Diverse areas are covered such as protective medicine, exercise physiology, mitochondrial pharmacology, aging, and comparative mitochondrial physiology (cell types, tissues, species). The common focus is on methodology, experimental details and quality control. The MitoFit Science Camp is a student- and research-oriented even, and may be considered as a sequence of workshops rather than a conference or school, addressing key topics of the COST Action MitoEAGLE project. This will provide a key opportunity to prepare the 1^st Management Committee Meeting.

• O2k-Network Lab: AT Innsbruck Oroboros

Labels: MiParea: mt-Awareness 

2016, ORO, O2k-Network, MitoFit 

MitoFit Science Camp - for and with mitochondrial scientists

Date, venue, sports programme

Date

2016, July 07-13

Immediately following EBEC2016 (July 2-7) organized by Paolo Bernardi in Riva del Garda (2.5 hours from Innsbruck).

Venue

High Altitude Training Center Kühtai, Kuehtai (Tyrol, Austria), 2020 m a.s.l. (42 min from Innsbruck).

Scientific sessions

Sporthotel Kühtai

A-6183 Kühtai • Nr. 9

www.sporthotel-kuehtai.com

Accommodation, breakfast and dinner

Hotel Astoria

A-6183 Kühtai • Nr. 33

Tel.: +43 5239-5215 • Fax: +43 5239-5215-80 • Mail [email protected]

www.hotelastoria.at

• Additional accomodation: sourrounding apartments/hotels.

Sports programme

• Activities at the participant's own risk. Sport programme is offered in collaboration with the Institute of Sport Science (University Innsbruck), Sporttherapie Huber, and the High Altitude Training Centre Kühtai.
• Sign in as a subject: spiroergometric tests (limited number of participants).
• MitoFit: expertise by experience - high altitude sports and physiotherapy in the Kuehtai environment.

 » http://www.hoehentraining-kuehtai.at/

 » http://www.sporttherapie-huber.at

» Meet the MitoFit athlete: a unique training experience

» Meet the MitoFit expert: groups and one-on-one sessions

» Walks&Talks

Feedback

• First I want to congratulate you for an excellent meeting and a great opportunity to start organizing the eCOST MitoEAGLE. - Pablo M Garcia-Roves, O2k-Network Lab: ES Barcelona Garcia-Roves PM
• Participating at the workshop was very interesting and I enjoyed it a lot. Thank you for the perfect organisation. - Carmina Silaidos, O2k-Network Lab: DE Frankfurt Eckert GP
• I want to say thank you to the whole Oroboros Team, and of course especially to Erich!! - Chiara Volani
• Congratulations again to a great meeting - a successful approach to 'targeting, body & mind' - Dominik Pesta, O2k-Network Lab: DE Duesseldorf Roden M
• Thank you very much for the well organised and very interesting MitoFit Science Camp. I am really happy that I could take part in it! - Dita Pelnena
• Thanks so much! Really appreciate it. Thank you for the opportunity to present. Was a great meeting! - Nicole Bezuidenhout, O2k-Network Lab: ZA Cape Town Ojuka EO
• Congratulations to you and the Oroboros crew for an interesting and spirited MitoFit Science Camp! - Daniel Kane, O2k-Network Lab: CA Antigonish Kane DA
• Thank you for an awesome conference, it was a delight and honour to have presented my work! - Prisca Osiki, O2k-Network Lab: ZA Cape Town Ojuka EO
• The workshop was great! I had a lot of fun and everything was very organized. - Chantal Pileggi, O2k-Network Lab: NZ Auckland Hickey AJ
• I thank you very much for this outstanding, perfectly organized science camp. It was a great opportunity for me to participate in order to start networking with "mitochondria people", getting to know the Oxygraph, methods, protocols. I am well certain that soon I will be also a user of the Oroboros instrument as well. - Krisztina Verébné Tar
• Again thank you for the very nice organisation of this meeting. Everything was perfect scientifically. - Thierry Arnould
• The meeting was fantastic as always! - Charles L Hoppel, O2k-Network Lab: US OH Cleveland Hoppel CL

Impressions from the MitoFit Science Camp 2016

(c) Erich Gnaiger and Verena Laner, if not stated otherwise in the additional information to the specific picture.

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  The first MitoFit Science Camp in Kuehtai with nearly 60 participants is going to start.

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  Erich Gnaiger giving a lecture on the O2k and the latest scientific results.

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  Carolina Doerrier, Oroboros Instruments, preparing chemicals for the demo experiment with reference protocol 1 & 2.

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  Some very diligent and hardworking participants - doing the DL7 proficiency test.

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  Erich Gnaiger introducing the beginners to DatLab7 in an evening session.

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  Having lots of fun with a volleyball tournament - a very pleasant activity.

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  All participants in action, taking the tournament very seriously.

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  All playing with full physical implication and high enthusiasm.

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  Concentration during service.

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  Stephen Dozier full in action.

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  Michaela Markova is highly delighted, Verena Laner in the background.

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  Team meeting on the sports ground and discussing the strategy.

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  Other participants relaxing by playing football - taking part in their own UEFA European Championship.

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  Let a new day start - Yoga-morning-session with Nicole Bezuidenhout on the Hotel's terrace.

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  Johannes Burtscher, Patrick Schaefer, Luiz Felipe Garcia e Souza, Pablo Garcia-Roves, Enrico Calzia, Stephen Dozier, Ignazio Prieto, Verena Laner, Erich Gnaiger, Devin Manning, Pedro Neves after the Lunch Action.

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  Johannes Burtscher giving an interesting lecture on his results about oxidative phosphorylation in the healthy and epileptic mouse brain.

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  Pablo Garcia-Roves talking about the technical perspective of high-resolution respirometry in permeabilized skinned muscle fibers from different mouse models.

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  David Marcinek showing his results on mitochondrial redox stress.

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  Wonderful view down to Kuehtai with a fantastic natural surrounding.

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  Beautiful alpine roses, a typical vegetation in this alpine area.

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  Due to a perfect sunny Sunday the excursion to the Dreiseen Hütte can take place.

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  Chantal Pileggi, Dita Pelnena, Ed Ojuka and Pablo Garcia-Roves enjoying the beautiful and impressing surrounding.

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  Gloria-Maria Keppner and Adela Krajcova in front of the lake called Hirschebensee at a height of 2,164 m.

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  Taking pictures in this amazing natural environment is a 'must'.

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  Some brave participants used this lake for cooling down and enjoying the bath in this alpine lake (16°C).

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  A perfect snapshot of Erich head jumping into the water.

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  Zuzana Sumbalova, Elisa Calabria and Kristen Scholten enjoying this sunny day.

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  Verena Laner, Valentina Dikova, Carolina Doerrier and Chiara Volani.

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  Anthony Molina in front of an impressive alpine territory.

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  Finally arrived at the Dreiseen Hütte.

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  Everybody relaxing at the Dreiseen Hütte.

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  Michaela Markova taking a rest, well-deserved.

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  A pleasant sitting-together of mitochondriacs.

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  Another relaxed round-table: David Marcinek, P. Darrell Neufer with his wife Robyn and Justin Perry.

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  Beautiful view

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  Erich and his piece of art.

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  Watching the final game of the European soccer championship.

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  Pablo Garcia-Roves giving his lecture on design and implementation of systems biology approaches to integrate heterogenic data in biomedical research.

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  Michael Drinnan letting Datlab out of its box and talking about his visions with the software.

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  Martin Burtscher trying to answer the question whether living and exercising at moderate altitude is health risk or even benefit.

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  Meagan McManus talking about a multi-tiered mitochondrial approach to predictive biomarkers of neurodegenerative disease.

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  If muscle mitochondrial adaptations occur in response to high altitude is answered by Adam Chicco.

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  Gerald Maarman explaining that melatonin rescues uric acid-induced impairment of respiration in myotubes.

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  Training session with Stefan Moser (Sporttherapie Mag. Huber, AT).

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  Pedro and Ignazio being highly concentrated with this exercise.

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  Dita, Zuzana and Johannes carefully following the instructions by Stefan.

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  Up and down by Ignazio and Pedro.

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  Johannes and Erich performing a demanding exercise.

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  Patrick and Enrico enjoy playing with the ball.

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  Trainer Stefan carefully watching the performance of Pedro and Ignazio.

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  Dita and Zuzana trying to do the exercise precisely.

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  Energetic ball playing of Johannes Burtscher and Erich Gnaiger.

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  How should this exercise be done?

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  Verena Laner and Pedro Neves proudly showing the MitoFit-logo.

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  Part of the MitoFit-family: Verena Laner, Carolina Doerrier and Georgia Lamberti from Oroboros Instruments.

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  Brian Irving, Verena Laner, Adela Krajcova, Michaela Markova, Anthony Molina and Gloria-Maria Keppner enjoying the MitoFit Science Camp.

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  Back to work.

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  Giorgia Lamberti explaining her measurements and results.

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  Chiara Volani and Carolina Doerrier working on the DL7 proficiency test.

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  Gerald Maarman and Prisca Osiki.

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  Another scientific meeting in private between Patrick Schaefer, Devin Manning and Luiz Felipe Garcia e Souza.

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  Exchange between O2k-users.

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  Gloria-Maria Keppner and Michaela Markova working late night.

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  Enrico Calzia, Dominik Pesta and Tomas Jelenik.

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  Showing measurements on HEK-cells - Erich Gnaiger and Giorgia Lamberti (Oroboros Instruments).

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  The MitoFit Science Camp-Team.

Oroboros Scholarships

An Oroboros scholarship has been awarded to the following persons for the presentation of his/her research:

Michaela Markova (Charles University in Prague, CZ)

Adela Krajcova (Charles University in Prague, CZ)

Patrick Schäfer (University of Ulm, DE)

Dita Pelnena (Latvian Biomedical Research and Study Centre, Riga, LV)

Norma Lilia Morales Garcia (National University of Mexico, Mexico City, MX)

Christina Uribe Alvarez (National University of Mexico, Mexico City, MX)

Chantal Pileggi (University of Auckland, NZ)

Nicole Bezuidenhout (University of Cape Town, ZA)

Prisca Osiki (University of Cape Town, ZA)

Poster Awards

Poster Awards were presented to the following persons in recognition of their outstanding posters:

• Dita Pelnena (Latvian Biomedical Research and Study Centre, Riga, LV): OXPHOS enzyme activity measurements in mitochondria isolated from peripheral blood leukocytes in control group and patients with suspected mitochondrial disease.
• Chao-Pin Hsiao (Case Western Reserve University School of Medicine, Cleveland, OH, US): Association between mitochondrial bioenergetics and radiation-related fatigue: a possible mechanism and novel target.
• Anna Herminghaus (University of Duesseldorf, DE): Sterile laparotomy time-dependently modulates hepatic but not colonic mitochondrial function similar to moderate abdominal sepsis.
• Chantal Pileggi (University of Auckland, NZ): Voluntary exercise prevents high fat diet-induced cardiac mitochondrial dysfunction in male rats.
• Michaela Markova (Charles University in Prague, CZ): Porcine hearts samples preparation: PBI-Shredder or saponin?

Participants, abstracts, programme

» MitoFit SC 2016 Participants and abstracts.

» Programme

A1: Diagnostic substrate-uncoupler-inhibitor titration (SUIT) protocols of mitochondrial fitness.

A2: Quality management system for mitochondrial respirometry: the MitoFit proficiency test with a real-time Demo-Experiment (SUIT reference protocol).

A3: DatLab 7 proficiency test

B1: Mitochondrial assays in blood cells: Blood cell preparation, cryopreservation, respiration.

B2: Cell culture models versus tissue samples: - Brain and neuronal cells - Adipose tissue - Other.

B3: Mitochondrial studies in neurodegeneration.

C1: Skeletal and cardiac muscle fibres - towards a data repository.

Working groups will be defined for the following topics: hyperoxic vs. normoxic, media, buffers, preparational assays, dry weight vs. wet weight, normalization (tissue vs. mt-markers; flux control ratios; flux control factors; statistical critique of RCR), quality control and nomenclature.

C2: Excursion: Dreiseenhütte.

D1: Towards a database on mt-respiratory physiology: from laboratory standards to inter-laboratory harmonization.

D2: Mitochondrial health: molecules, cells and tissues.

D3: MoTrPAC and MitoEAGLE

E1: MitoEAGLE and parallel IOC112 O2k-Workshop.

E2: MitoEAGLE and parallel IOC112 O2k-Workshop.

July	07 Th	08 Fr	09 Sa	10 Su	11 Mo	12 Tu	13 We
07:00-08:00	Arrival	Yoga with Meagan	Yoga with Meagan		MitoFit training I with Stefan	HIT-Training with Verena
08:00-09:00		Break(fast)	Break(fast)	Break(fast)	Break(fast)	Break(fast)	Breakfast
09:00-10:15		Topic A1	Topic B1	Topic C1	Topic D1	Topic E1, VO2max tests	Departure
10:45-12:00
12:00-15:00		Lunch & Action / e-bikes	Soccer / Volleyball / e-bikes	Excursion to Dreiseenhütte in power / moderate / slow mode or Innsbruck (bad weather alternative)	Volleyball / Athletics / MitoFit Training II with Stefan, e-bikes, VO2max tests	2 km walking-tests, VO2max tests, e-bikes
15:30-16:45		Topic A2	Topic B2		Topic D2	Topic E2
17:15-18:30
19:00-20:30	Dinner	Dinner	Dinner	Dinner	Dinner	Dinner
20:30-21:15	Welcome by the organizers	A3: DatLab 7 proficiency test	B3: Evening topic	European-Championship public viewing	D3: MitoEAGLE and MoTrPAC	MitoFit party

• Topics are scheduled as a sequence of workshops to merge introductory lectures, panels, and poster flashes.
• High-resolution respirometry (HRR) will be demonstrated at various levels, integrating an IOC (O2k-Workshop on HRR) into the MitoFit Science Camp, with an emphasis on instrumental quality control - towards MitoFit Proficiency Testing. DatLab 7 will be released and introduced as the software for quality control in HRR.
• The programme is designed as a global introduction to the perspectives of the COST Action MitoEAGLE.

MitoEAGLE theme

MitoEAGLE aims at providing the first standardized measures to link mitochondrial and physiome performance (heart rate, BMI, blood pressure, blood chemistry, V_O2max) to understand the myriad of factors that play a role in mitochondrial physiology. The ambitious objective to optimally harmonize protocols across research groups cannot be achieved through projects with limited numbers of partners. Therefore, the COST framework represents an ideal instrument which allows bringing together many groups and experts in the diverse field of mitochondrial physiology. The aim of this Action is to form a unique well-coordinated network of senior researchers and young investigators, to include well established stakeholders, and to establish a spirit of mentorship and collaboration in contrast to fierce competition which characterized early decades of bioenergetics. The COST Action MitoEAGLE aims at developing a quality management system (QMS), defining common standards for measurements in the field of mitochondrial function.- MitoEAGLE may be seen as too ambitious in the short term, but indispensable at large.

Increase the value and reduce the noise

Enthusiastic and authentic opinion leaders can bring about a change to increase the value and reduce the noise in mitochondrial research specifically, biomedicine at large, and in our society in general. Millions of Euros are wasted on research that is lacking coherent standards. Prespecified and time-stamped protocols are needed, and researchers need to be introduced into adhering to publicly deposited protocols in practice (Begley, Ioannidis 2015). MitoEAGLE recommendations will provide practical guidelines for students, scientists and stakeholders.

Further details: MitoEAGLE

» For the programme in detail please see: MitoFit_SC_2016_Programme information

MitoEAGLE: towards the 1^st MC Meeting

• List of MC Members: http://www.cost.eu/COST_Actions/ca/CA15203?management

The sessions on the COST Action MitoEAGLE at the MitoFit Science Camp provided an excellent opportunity to discuss some key topics in preparation of the 1^st Management Committee (MC) Meeting, including several Management Committee Members who participated in the full MitoFit SC or joined specifically for this discussion.

The Working Groups (WG) were divided into Task Groups. This was particularly important for the large WG1 (Standard operating procedures and user requirement document: Protocols, terminology, documentation). The tentative WG5 (Dissemination and training) could thereby be incorporated into WG1.

Chair, Vicechair and Steering Committee

All suggestions may be turned over at the 1st Management Committee Meeting.

Among the participants, assuming that the Main Proposer (Erich Gnaiger) will be nominated as Chair of the COST Action MitoEAGLE, Elisa Calabria was suggested as the Vicechair to be nominated at the 1^st MC Meeting.

Steering Committee

A Steering Committee should be nominated, consisting of the Chair, Vicechair, WG leaders, regional representatives (not MC members) beyond the limits of COST countries, a Financial Officer (Jaap Keijer should be consulted for suggestions), and a General Secretary (Christina Plattner).

The following criteria should be kept in mind for nominations: (1) Expertise; (2) Regions - add non-MC members as links to other continents; (3) Gender balance; (4) participation of Early Career Investigators (researchers in the time span of up to 8 years after the date of obtaining the PhD/doctorate). Among the participants, the following WG leaders were suggested, and a co-leader should be additionally nominated for the 1^st MC Meeting:

WG leaders

• 2016-09-06: Erich is attending the COST Preparatory Workshop for Main Proposers at the COST Office in Brussels. Here are some relevent suggestions from COST:

• WG leaders have to be MC members, exceptionally MC substitute.
• Colleagues from Inclusiveness Target Countries (ITC) and Early Career Investigator (ECI) should be well represented as WG leaders, and rotation of some MC members for ECI should be considered by some COST National Coordinators.
• A rotational system can be implemented and is even encouraged. WG co-leaders may be elected at the MC meeting with the intention to rotate at a given time point between WG leader and WG co-leader. Both should be MC members.
• Define in advance Short Term Scientific Missions with ITC and ECI in mind, decide on assessment criteria.

• WG1: Kathrin Renner-Sattler
• WG2: Marina Makrecka-Kuka
• WG3: Vilma Borutaite - (not present)
• WG4: Enrico Calzia (but: Enrico is not a MC member - Enrico is of course welcome as a WG participant)
• WG5: will be incorporated into WG1

International partner representatives

• Bret H Goodpaster (North America)
• Anthony J Hickey (Australasia)
• Anibal Vercesi (South America) - (not present)
• Edward O Ojuka (Africa) - (not present: Sameh Ali)
• Central Asia
• Southeast Asia

Working groups with a list of task forces

WG1: MitoEAGLE protocols, terminology, documentation

• Coupling states and coupling control ratios: Consensus statements. – Kathrin Renner-Sattler / Erich Gnaiger / Charles L Hoppel
• SUIT protocols: nomenclature consistent with concept of database. Erich Gnaiger / ...
• Experimental details in SUIT. Adam Chicco / Anthony J Hickey / Carolina Doerrier-Velasco
• Instrumental platforms: comparisons. – Anthony J Molina / (not present: Carlos Canto / Antonio Zorzano / Karl Johan Tronstad)
• Knowledge management system. – Michael Drinnan / Christina Plattner
• Proficiency test: Erich Gnaiger / Carolina Doerrier-Velasco
• Documentation: Thierry Arnould / Dominique-Marie Votion
• Dissemination Scientific community: Erich Gnaiger / Marina Makrecka-Kuka
• Translation to community in general: Brian A Irving / ...
• Training, Short Term Scientific Missions: (not present: Susanne Klaus)

WG2: MitoEAGLE data: muscle

• Skeletal: Pablo M Garcia-Roves / Dominique-Marie Votion / Paul Coen
• Cardiac: Terje S Larsen / Marina Makrecka-Kuka

WG3: MitoEAGLE data: tissues

• Fat: (not present: Jan Nedergard)
• Liver: (not present: Zuzana Cervinkova) / Zuzana Sumbalova
• Neuronal: (not present: Carmen Sandi / Vilma Borutaite)

WG4: MitoEAGLE data: blood and cultured cells

• Blood: Elisa Calabria / Enrico Calzia
• Cultured cells: Kathrin Renner-Sattler / ...

Partners

Additional support

Listed under MitoGlobal Events.