Development MitoFit proficiency test

From Bioblast

Bioblast 2022

Development MitoFit proficiency test

The project MitoFit highlights the benefits of mitochondrial fitness.

MitoFit Workpackages


Development of the MitoFit proficiency test


A proficiency test is an essential part of quality control designed to minimize experimental error in diagnostic tests. Instrumental errors can be detected by routine calibration and instrumental performance tests (instrumental background) and avoided by technical service before conducting a diagnostic test, should instrumental validation indicate a problem in advance of an experiment (WP3). In addition, reference samples with properties relevant for the diagnostic assay (WP4) are analysed at different time points during the longitudinal progress of a project or in the direct context of patient diagnostic assays, such that quantitative results are validated. The purpose of the proficiency test is also to help improve performance and to determine if the test methods in use are reliable and reproducible (Figure 6A). Interlaboratory ring testing of reference samples is known as an external quality control (Figure 6B). The need for such a proficiency testing scheme for respiratory OXPHOS analysis is frequently discussed in the literature, but no generally applicable solution is available yet. An interlaboratory comparison revealed disturbingly high variability of results with spectrophotometric (enzymatic) analysis of respiratory complex activities (Gellerich et al 2004). In contrast to respiratory OXPHOS analysis, enzyme kinetic OXPHOS assays are routinely performed on frozen samples. The interlaboratory MitoFit-PT will be developed and introduced in practice as a world-wide innovation in the field of mitochondrial respiratory physiology. A proficiency ring test is obligatory for certified laboratories.

Progress and next steps

Β» MiPNet21.07 MitoFit DatLab PT
Β» MitoFit Science Camp 2016 Kuehtai AT
Β» MiPNet21.06 SUIT RP

References and links

Β» ISO/IEC 17043:2010 General requirements for proficiency testing
Β» ISO 13528:2015 Statistical methods for use in proficiency testing by interlaboratory comparison
Β» COST MitoEAGLE project
Β« Back to overview
Cookies help us deliver our services. By using our services, you agree to our use of cookies.