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Difference between revisions of "MitoFit Science Camp 2016 Kuehtai AT"

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{{MitoFit page name}}
{{Oroboros highlights page name}}
{{Publication
{{Publication
|title=[[Image:Logo MitoFit.jpg|100px|right|link=http://www.mitofit.org/index.php/MitoFit|MitoFit]]
|title=[[Image:Logo MitoFit.jpg|100px|right|link=http://www.mitofit.org/index.php/MitoFit|MitoFit]]
'''Kuehtai AT''', 2016 Jul 07-13. '''MitoFit Science Camp''' and '''IOC112'''.
'''Kuehtai AT''', 2016 Jul 07-13. '''MitoFit Science Camp''' and '''IOC112'''.
|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/index.php/File:MiPNet21.15_MitoFit-SC_IOC112.pdf |Bioblast pdf]]
|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/a/a2/MiPNet21.15_MitoFit-SC_IOC112.pdf |Bioblast pdf]]
|authors=OROBOROS
|authors=Oroboros
|year=2016-07-07
|year=2016-07-07
|journal=MitoGlobal
|journal=MitoGlobal
|abstract='''2016 Jul 07-13, Kuehtai, AT.'''  
|abstract='''2016 Jul 07-13, Kuehtai, AT.'''  
The project [[K-Regio MitoFit |MitoFit]] aims at developing novel laboratory standards and diagnostic monitoring of a mitochondrial fitness score. With an international team of outstanding mitochondrial experts, the '''MitoFit Science Camp''' will provide a unique opportunity to receive first-hand introductions to state-of-the-art diagnostic monitoring of mitochondrial respiratory function combined with hands-on training on HRR (IOC112). Diverse areas are covered such as protective medicine, exercise physiology, mitochondrial pharmacology, aging, and comparative mitochondrial physiology (cell types, tissues, species). The common focus is on methodology, experimental details and quality control. The MitoFit Science Camp is a student- and research-oriented even, and may be considered as a sequence of workshops rather than a conference or school, addressing key topics of the COST Action [[MITOEAGLE]] project. This will provide a key opportunity to prepare the [[COST Action MITOEAGLE |1<sup>st</sup> Management Committee Meeting]].
The project [[K-Regio MitoFit |MitoFit]] aims at developing novel laboratory standards and diagnostic monitoring of a mitochondrial fitness score. With an international team of outstanding mitochondrial experts, the '''MitoFit Science Camp''' will provide a unique opportunity to receive first-hand introductions to state-of-the-art diagnostic monitoring of mitochondrial respiratory function combined with hands-on training on HRR (IOC112). Diverse areas are covered such as protective medicine, exercise physiology, mitochondrial pharmacology, aging, and comparative mitochondrial physiology (cell types, tissues, species). The common focus is on methodology, experimental details and quality control. The MitoFit Science Camp is a student- and research-oriented even, and may be considered as a sequence of workshops rather than a conference or school, addressing key topics of the COST Action [[MitoEAGLE]] project. This will provide a key opportunity to prepare the [[COST Action MitoEAGLE |1<sup>st</sup> Management Committee Meeting]].
|mipnetlab=AT Innsbruck OROBOROS
|mipnetlab=AT Innsbruck Oroboros
}}
}}
{{Labeling
{{Labeling
|area=mt-Awareness
|area=mt-Awareness
|additional=2016, ORO, O2k-Network, MitoFit, Next
|additional=2016, ORO, O2k-Network, MitoFit
}}
}}




[[Image:Logo MitoFit-OROBOROS.jpg|right|300px|link=http://www.mitofit.org/index.php/MitoFit |MitoFit]]
[[Image:Logo MitoFit-OROBOROS.jpg|right|300px|link=http://www.mitofit.org/index.php/MitoFit |MitoFit]]
[[Image:SC2016 (16).JPG|right|300px| Johannes Burtscher, Patrick Schaefer, Luiz Felipe Garcia e Souza, Pablo Garcia-Roves, Enrico Calzia, Stephen Dozier, Ignazio Prieto, Verena Laner, Erich Gnaiger, Devin Manning, Pedro Neves]]
__TOC__
__TOC__


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:::: High Altitude Training Center Kühtai, Kuehtai (Tyrol, Austria), 2020 m a.s.l. (42 min from Innsbruck).
:::: High Altitude Training Center Kühtai, Kuehtai (Tyrol, Austria), 2020 m a.s.l. (42 min from Innsbruck).


:::: [[File:HLZ-Kuehtai 9804.jpg |300px|right|link=http://www.hoehentraining-kuehtai.at/]]
:::: [[File:Lemawork Kuehtai-MitoFit.jpg |300px|right||Lemawork Katema training in Kuehtai]]


'''Scientific sessions'''
'''Scientific sessions'''
:::: '''Sporthotel Kühtai'''
:::: '''Sporthotel Kühtai'''
:::: A-6183 Kühtai • Nr. 9
:::: A-6183 Kühtai • Nr. 9
:::: [http://www.sporthotel-kuehtai.com/index.php?id=114&L=1 www.sporthotel-kuehtai.com]
:::: [http://www.sporthotel-kuehtai.com/ www.sporthotel-kuehtai.com]


'''Accommodation, breakfast and dinner'''
'''Accommodation, breakfast and dinner'''
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'''Sports programme'''
'''Sports programme'''
::::* Activities at the participant's own risk. Sport programme is offered in collaboration with the Institute of Sport Science (University Innsbruck), Sporttherapie Huber, and the High Altitude Training Centre Kühtai.
::::* Activities at the participant's own risk. Sport programme is offered in collaboration with the Institute of Sport Science (University Innsbruck), Sporttherapie Huber, and the High Altitude Training Centre Kühtai.
:::* Sign in as a '''subject''': spiroergometric tests (limited number of participants).
::::* Sign in as a '''subject''': spiroergometric tests (limited number of participants).
:::* MitoFit: expertise by '''experience''' - high altitude sports and physiotherapy in the Kuehtai environment.
::::* MitoFit: expertise by '''experience''' - high altitude sports and physiotherapy in the Kuehtai environment.
:::: [[File:Logo_HLZ-Kuehtai.jpg|40px|link=http://www.hoehentraining-kuehtai.at/|HLZ Kuehtai]] » http://www.hoehentraining-kuehtai.at/
:::: [[File:Logo_HLZ-Kuehtai.jpg|40px|link=http://www.hoehentraining-kuehtai.at/|HLZ Kuehtai]] » http://www.hoehentraining-kuehtai.at/
:::: [[File:Logo_Sporttherapie-Huber.png |40px|link=http://www.sporttherapie-huber.at|Sporttherapie Huber]] » http://www.sporttherapie-huber.at
:::: [[File:Logo_Sporttherapie-Huber.png |40px|link=http://www.sporttherapie-huber.at|Sporttherapie Huber]] » http://www.sporttherapie-huber.at
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== Feedback ==
== Feedback ==
::::* ''First I want to congratulate you for an excellent meeting and a great opportunity to start organizing the eCOST MITOEAGLE.'' - '''[[Garcia-Roves PM|Pablo M Garcia-Roves]]''', O2k-Network Lab: [[ES Barcelona Garcia-Roves PM]], [[ES Barcelona IDIBAPS Hospital Clinic]]   
::::* ''First I want to congratulate you for an excellent meeting and a great opportunity to start organizing the eCOST MitoEAGLE.'' - '''[[Garcia-Roves PM|Pablo M Garcia-Roves]]''', O2k-Network Lab: [[ES Barcelona Garcia-Roves PM]]   
::::* ''Participating at the workshop was very interesting and I enjoyed it a lot. Thank you for the perfect organisation.''  - '''[[Schaefer C|Carmina Schaefer]]''', O2k-Network Lab: [[DE Frankfurt Eckert GP]]
::::* ''Participating at the workshop was very interesting and I enjoyed it a lot. Thank you for the perfect organisation.''  - '''[[Silaidos C|Carmina Silaidos]]''', O2k-Network Lab: [[DE Frankfurt Eckert GP]]
::::* ''I want to say thank you to the whole Oroboros Team, and of course especially to Erich!!''  - '''[[Volani C|Chiara Volani]]'''
::::* ''I want to say thank you to the whole Oroboros Team, and of course especially to Erich!!''  - '''[[Volani C|Chiara Volani]]'''
::::* ''Congratulations again to a great meeting - a successful approach to 'targeting, body & mind'''  - '''[[Pesta D|Dominik Pesta]]''', O2k-Network Lab: [[DE Duesseldorf Roden M]]
::::* ''Congratulations again to a great meeting - a successful approach to 'targeting, body & mind'''  - '''[[Pesta D|Dominik Pesta]]''', O2k-Network Lab: [[DE Duesseldorf Roden M]]
::::* ''Thank you very much for the well organised and very interesting MitoFit Science Camp. I am really happy that I could take part in it!''  - '''[[Pelnena D|Dita Pelnena]]'''
::::* ''Thanks so much! Really appreciate it. Thank you for the opportunity to present. Was a great meeting!''  - '''[[Bezuidenhout N|Nicole Bezuidenhout]]''', O2k-Network Lab: [[ZA Cape Town Ojuka EO]]
::::* ''Congratulations to you and the Oroboros crew for an interesting and spirited MitoFit Science Camp!''  - '''[[Kane DA|Daniel Kane]]''', O2k-Network Lab: [[CA Antigonish Kane DA]]
::::* ''Thank you for an awesome conference, it was a delight and honour to have presented my work!''  - '''[[Osiki P|Prisca Osiki]]''', O2k-Network Lab: [[ZA Cape Town Ojuka EO]]
::::* ''The workshop was great! I had a lot of fun and everything was very organized.''  - '''[[Pileggi C|Chantal Pileggi]]''', O2k-Network Lab: [[NZ Auckland Hickey AJ]]
::::* ''I thank you very much for this outstanding, perfectly organized science camp. It was a great opportunity for me to participate in order to start networking with "mitochondria people", getting to know the Oxygraph, methods, protocols. I am well certain that soon I will be also a user of the Oroboros instrument as well.'' - '''[[Verebne Tar K|Krisztina Verébné Tar]]
::::* ''Again thank you for the very nice organisation of this meeting. Everything was perfect scientifically.''  - '''[[Arnould T|Thierry Arnould]]'''
::::* ''The meeting was fantastic as always!'' - '''[[Hoppel CL|Charles L Hoppel]]''', O2k-Network Lab: [[US OH Cleveland Hoppel CL]]




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<gallery mode=default perrow=3 widths="300px" heights="300px">
<gallery mode=default perrow=3 widths="300px" heights="300px">
File:SC2016 (1).JPG|The first MitoFit Science Camp in Kuehtai with nearly 60 participants is going to start.
File:SC2016 (1).JPG|The first MitoFit Science Camp in Kuehtai with nearly 60 participants is going to start.
File:SC2016 (2).JPG|Erich Gnaiger giving his first lecture on O2k and the latest scientific results.
File:SC2016 (2).JPG|Erich Gnaiger giving a lecture on the O2k and the latest scientific results.
File:SC2016 (4).JPG|Carolina Doerrier, OROBOROS INSTRUMENTS, preparing chemicals for the demo experiment with reference protocol 1 & 2.
File:SC2016 (4).JPG|Carolina Doerrier, Oroboros Instruments, preparing chemicals for the demo experiment with reference protocol 1 & 2.
File:SC2016 (5).JPG|Some very diligent and hardworking participants - doing the DL7 proficiency test.
File:SC2016 (5).JPG|Some very diligent and hardworking participants - doing the DL7 proficiency test.
File:SC2016 (6).JPG|Erich Gnaiger introducing the beginners to DatLab7 in an evening session.
File:SC2016 (6).JPG|Erich Gnaiger introducing the beginners to DatLab7 in an evening session.
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File:SC2016 (14).JPG|Other participants relaxing by playing football - taking part in their own UEFA European Championship.
File:SC2016 (14).JPG|Other participants relaxing by playing football - taking part in their own UEFA European Championship.
File:SC2016 (15).JPG|Let a new day start - Yoga-morning-session with Nicole Bezuidenhout on the Hotel's terrace.
File:SC2016 (15).JPG|Let a new day start - Yoga-morning-session with Nicole Bezuidenhout on the Hotel's terrace.
File:SC2016 (16).JPG|Johannes Burtscher, Patrick Schaefer, Luiz Felipe Garcia e Souza, Pablo Garcia-Roves, Enrico Calzia, Stephen Dozier, Ignacio Prieto, Verena Laner, Erich Gnaiger, Devin Manning, Pedro Neves after the Lunch Action.
File:SC2016 (16).JPG|Johannes Burtscher, Patrick Schaefer, Luiz Felipe Garcia e Souza, Pablo Garcia-Roves, Enrico Calzia, Stephen Dozier, Ignazio Prieto, Verena Laner, Erich Gnaiger, Devin Manning, Pedro Neves after the Lunch Action.
File:SC2016 (17).JPG|Johannes Burtscher giving an interesting lecture on his results about oxidative phosphorylation in the healthy and epileptic mouse brain.
File:SC2016 (17).JPG|Johannes Burtscher giving an interesting lecture on his results about oxidative phosphorylation in the healthy and epileptic mouse brain.
File:SC2016 (18).JPG|Pablo Garcia-Roves talking about the technical perspective of high-resolution respirometry in permeabilized skinned muscle fibers from different mouse models.
File:SC2016 (18).JPG|Pablo Garcia-Roves talking about the technical perspective of high-resolution respirometry in permeabilized skinned muscle fibers from different mouse models.
File:SC2016 (19).JPG|Paul Coen presenting a quantification on improved mitochondrial function in human skeletal muscle.
File:SC2016 (20).JPG|David Marcinek showing his results on mitochondrial redox stress.
File:SC2016 (20).JPG|David Marcinek showing his results on mitochondrial redox stress.
File:SC2016 (21).JPG|Wonderful view down to Kuehtai with a fantastic natural surrounding.
File:SC2016 (21).JPG|Wonderful view down to Kuehtai with a fantastic natural surrounding.
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File:SC2016 (23).JPG|Due to a perfect sunny Sunday the excursion to the Dreiseen Hütte can take place.
File:SC2016 (23).JPG|Due to a perfect sunny Sunday the excursion to the Dreiseen Hütte can take place.
File:SC2016 (24).JPG|Chantal Pileggi, Dita Pelnena, Ed Ojuka and Pablo Garcia-Roves enjoying the beautiful and impressing surrounding.
File:SC2016 (24).JPG|Chantal Pileggi, Dita Pelnena, Ed Ojuka and Pablo Garcia-Roves enjoying the beautiful and impressing surrounding.
File:SC2016 (25).JPG|What are these guys talking about? Brian Irving, Paul Coen, Erich Gnaiger and David Marcinek.
File:SC2016 (26).JPG|Gloria-Maria Keppner and Adela Krajcova in front of the lake called Hirschebensee at a height of 2,164 m.
File:SC2016 (26).JPG|Gloria-Maria Keppner and Adela Krajcova in front of the lake called Hirschebensee at a height of 2,164 m.
File:SC2016 (27).JPG|Taking pictures in this amazing natural environment is a 'must'.
File:SC2016 (27).JPG|Taking pictures in this amazing natural environment is a 'must'.
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File:SC2016 (38).JPG|Beautiful view
File:SC2016 (38).JPG|Beautiful view
File:SC2016 (39).JPG|Erich and his piece of art.
File:SC2016 (39).JPG|Erich and his piece of art.
File:SC2016 (40).JPG|Back to work: Nicole Bezuidenhout presenting her results on the work with the permeabilized fibres and the cooling plate.
File:SC2016 (41).JPG|Watching the final game of the European soccer championship.
File:SC2016 (41).JPG|Watching the final game of the European soccer championship.
File:SC2016 (42).JPG|Pablo Garcia-Roves giving his lecture on design and implementation of systems biology approaches to integrate heterogenic data in biomedical research.
File:SC2016 (42).JPG|Pablo Garcia-Roves giving his lecture on design and implementation of systems biology approaches to integrate heterogenic data in biomedical research.
File:SC2016 (43).JPG|Michael Drinnan letting Datlab out of its box and talking about his visions with the software.
File:SC2016 (43).JPG|Michael Drinnan letting Datlab out of its box and talking about his visions with the software.
File:SC2016 (44).JPG|Martin Burtscher trying to answer the question whether living and exercising at moderate altidute is health risk or even benefit?
File:SC2016 (44).JPG|Martin Burtscher trying to answer the question whether living and exercising at moderate altitude is health risk or even benefit.
File:SC2016 (45).JPG|Meagan McManus talking about a multi-tiered mitochondrial approach to predictive biomarkers of neurodegenerative disease.
File:SC2016 (45).JPG|Meagan McManus talking about a multi-tiered mitochondrial approach to predictive biomarkers of neurodegenerative disease.
File:SC2016 (46).JPG|If muscle mitochondrial adaptations occur in response to high altitude is answered by Adam Chicco.
File:SC2016 (46).JPG|If muscle mitochondrial adaptations occur in response to high altitude is answered by Adam Chicco.
File:SC2016 (47).JPG|Gerald Maarman explaining that melatonin rescues uric acid-induced impairment of respiration in myotubes.
File:SC2016 (47).JPG|Gerald Maarman explaining that melatonin rescues uric acid-induced impairment of respiration in myotubes.
File:SC2016 (48).JPG|Bret Goodpaster talking about the NIH-funded project MoTrPAC.
File:SC2016 (49).JPG|Training session with Stefan Moser (Sporttherapie Mag. Huber, AT).
File:SC2016 (49).JPG|Training session with Stefan Moser (Sporttherapie Mag. Huber, AT).
File:SC2016 (50).JPG|Pedro and Ignazio being highly concentrated with this exercise.
File:SC2016 (50).JPG|Pedro and Ignazio being highly concentrated with this exercise.
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File:SC2016 (57).JPG|Energetic ball playing of Johannes Burtscher and Erich Gnaiger.
File:SC2016 (57).JPG|Energetic ball playing of Johannes Burtscher and Erich Gnaiger.
File:SC2016 (58).JPG|How should this exercise be done?  
File:SC2016 (58).JPG|How should this exercise be done?  
File:SC2016 (59).JPG|Sitting togehter of Paul, Darrell and Bret in the eveining, further discussing harmonization.
File:SC2016 (60).JPG|MitoFit branding of Pablo, Tony and Paul.
File:SC2016 (61).JPG|Verena Laner and Pedro Neves proudly showing the MitoFit-logo.
File:SC2016 (61).JPG|Verena Laner and Pedro Neves proudly showing the MitoFit-logo.
File:SC2016 (62).JPG|Part of the MitoFit-family: Verena Laner, Carolina Doerrier and Georgia Lamberti from OROBOROS INSTRUMENTS.
File:SC2016 (62).JPG|Part of the MitoFit-family: Verena Laner, Carolina Doerrier and Georgia Lamberti from Oroboros Instruments.
File:SC2016 (64).JPG|Brian Irving, Verena Laner, Adela Krajcova, Michaela Markova, Anthony Molina, Gloria-Maria Keppner enjoying the MitoFit Science Camp.
File:SC2016 (64).JPG|Brian Irving, Verena Laner, Adela Krajcova, Michaela Markova, Anthony Molina and Gloria-Maria Keppner enjoying the MitoFit Science Camp.
File:SC2016 (65).JPG|Back to work.  
File:SC2016 (65).JPG|Back to work.  
File:SC2016 (67).JPG|Giorgia Lamberti explaining her measurements and results.
File:SC2016 (67).JPG|Giorgia Lamberti explaining her measurements and results.
File:SC2016 (69).JPG|Chiara Volandi and Carolina Doerrier, busy with the DL7 proficiency test.
File:SC2016 (69).JPG|Chiara Volani and Carolina Doerrier working on the DL7 proficiency test.
File:SC2016 (70).JPG|Gerald Maarman and Prisca Osiki.   
File:SC2016 (70).JPG|Gerald Maarman and Prisca Osiki.   
File:SC2016 (71).JPG|Another scientific meeting in private between Patrick Schaefer, Devin Manning and Luiz Felipe Garcia e Souza.
File:SC2016 (71).JPG|Another scientific meeting in private between Patrick Schaefer, Devin Manning and Luiz Felipe Garcia e Souza.
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File:SC2016 (73).JPG|Gloria-Maria Keppner and Michaela Markova working late night.
File:SC2016 (73).JPG|Gloria-Maria Keppner and Michaela Markova working late night.
File:SC2016 (74).JPG|Enrico Calzia, Dominik Pesta and Tomas Jelenik.
File:SC2016 (74).JPG|Enrico Calzia, Dominik Pesta and Tomas Jelenik.
File:SC2016 (75).JPG|Showing measurements on HEK-cells - Erich Gnaiger and Giorgia Lamberti (OROBOROS INSTRUMENTS).
File:SC2016 (75).JPG|Showing measurements on HEK-cells - Erich Gnaiger and Giorgia Lamberti (Oroboros Instruments).
File:SC2016 (76).JPG|The MitoFit Science Camp-Team.
File:SC2016 (76).JPG|The MitoFit Science Camp-Team.


</gallery>
</gallery>
== Oroboros Scholarships ==
::: An Oroboros scholarship has been awarded to the following persons for the presentation of his/her research:
::: [[Markova M|Michaela Markova]] (Charles University in Prague, CZ)
::: [[Krajcova A|Adela Krajcova]] (Charles University in Prague, CZ)
::: [[Schaefer P|Patrick Schäfer]] (University of Ulm, DE)
::: [[Pelnena D|Dita Pelnena]] (Latvian Biomedical Research and Study Centre, Riga, LV)
::: [[Morales Garcia NL|Norma Lilia Morales Garcia]] (National University of Mexico, Mexico City, MX)
::: [[Uribe Alvarez C|Christina Uribe Alvarez]] (National University of Mexico, Mexico City, MX)
::: [[Pileggi C|Chantal Pileggi]] (University of Auckland, NZ)
::: [[Bezuidenhout N|Nicole Bezuidenhout]] (University of Cape Town, ZA)
::: [[Osiki P|Prisca Osiki]] (University of Cape Town, ZA)
== Poster Awards ==
::: Poster Awards were presented to the following persons in recognition of their outstanding posters:
:::* [[Pelnena D|Dita Pelnena]] (Latvian Biomedical Research and Study Centre, Riga, LV): [[Pelnena 2016 Abstract MitoFit Science Camp 2016|OXPHOS enzyme activity measurements in mitochondria isolated from peripheral blood leukocytes in control group and patients with suspected mitochondrial disease.]]
:::* [[Hsiao CP|Chao-Pin Hsiao]] (Case Western Reserve University School of Medicine, Cleveland, OH, US): [[Hsiao 2016 Abstract MitoFit Science Camp 2016|Association between mitochondrial bioenergetics and radiation-related fatigue: a possible mechanism and novel target.]]
:::* [[Herminghaus A|Anna Herminghaus]] (University of Duesseldorf, DE): [[Herminghaus 2016 Abstract MitoFit Science Camp 2016|Sterile laparotomy time-dependently modulates hepatic but not colonic mitochondrial function similar to moderate abdominal sepsis.]]
:::* [[Pileggi C|Chantal Pileggi]] (University of Auckland, NZ): [[Pileggi 2016 Abstract MitoFit Science Camp 2016|Voluntary exercise prevents high fat diet-induced cardiac mitochondrial dysfunction in male rats.]]
:::* [[Markova M|Michaela Markova]] (Charles University in Prague, CZ): [[Markova 2016 Abstract MitoFit Science Camp 2016|Porcine hearts samples preparation: PBI-Shredder or saponin?]]




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:::: '''A1''': Diagnostic substrate-uncoupler-inhibitor titration (SUIT) protocols of mitochondrial fitness.
:::: '''A1''': Diagnostic substrate-uncoupler-inhibitor titration (SUIT) protocols of mitochondrial fitness.
:::: '''A2''': Quality management system for mitochondrial respirometry: the [[MitoFit proficiency test]] with a real-time Demo-Experiment ([[SUIT reference protocol]]).
:::: '''A2''': Quality management system for mitochondrial respirometry: the [[Development MitoFit proficiency test| MitoFit proficiency test]] with a real-time Demo-Experiment ([[SUIT reference protocol]]).
:::: '''A3''': DatLab 7 proficiency test  
:::: '''A3''': DatLab 7 proficiency test  


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:::: '''D1''': Towards a database on mt-respiratory physiology: from laboratory standards to inter-laboratory harmonization.
:::: '''D1''': Towards a database on mt-respiratory physiology: from laboratory standards to inter-laboratory harmonization.
:::: '''D2''': Mitochondrial health: molecules, cells and tissues.
:::: '''D2''': Mitochondrial health: molecules, cells and tissues.
:::: '''D3''': MoTrPAC and MITOEAGLE  
:::: '''D3''': MoTrPAC and MitoEAGLE  


:::: '''E1''': MITOEAGLE and parallel IOC112 O2k-Workshop.
:::: '''E1''': MitoEAGLE and parallel IOC112 O2k-Workshop.
:::: '''E2''': MITOEAGLE and parallel IOC112 O2k-Workshop.
:::: '''E2''': MitoEAGLE and parallel IOC112 O2k-Workshop.




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| 19:00-20:30 || Dinner || Dinner || Dinner || Dinner || Dinner || Dinner  
| 19:00-20:30 || Dinner || Dinner || Dinner || Dinner || Dinner || Dinner  
|-
|-
| 20:30-21:15 || Welcome by the organizers || A3: DatLab 7 proficiency test || B3: Evening topic || European-Championship public viewing|| D3: MITOEAGLE and MoTrPAC || MitoFit party  
| 20:30-21:15 || Welcome by the organizers || A3: DatLab 7 proficiency test || B3: Evening topic || European-Championship public viewing|| D3: MitoEAGLE and MoTrPAC || MitoFit party  
|}
|}


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::::* Topics are scheduled as a sequence of workshops to merge introductory lectures, panels, and poster flashes.
::::* Topics are scheduled as a sequence of workshops to merge introductory lectures, panels, and poster flashes.
::::* High-resolution respirometry (HRR) will be demonstrated at various levels, integrating an IOC (O2k-Workshop on HRR) into the MitoFit Science Camp, with an emphasis on instrumental quality control - towards MitoFit Proficiency Testing. [[DatLab |DatLab 7]] will be released and introduced as the software for quality control in HRR.
::::* High-resolution respirometry (HRR) will be demonstrated at various levels, integrating an IOC (O2k-Workshop on HRR) into the MitoFit Science Camp, with an emphasis on instrumental quality control - towards MitoFit Proficiency Testing. [[DatLab |DatLab 7]] will be released and introduced as the software for quality control in HRR.
::::* The programme is designed as a global introduction to the perspectives of the COST Action [[MITOEAGLE]].
::::* The programme is designed as a global introduction to the perspectives of the COST Action [[MitoEAGLE]].


[[File:MITOEAGLE-MitoGlobal.jpg|right|400px|MiPArt Gallery: World health worries by Odra Noel (2012)]]
[[File:MITOEAGLE-MitoGlobal.jpg|right|400px|MiPArt Gallery: World health worries by Odra Noel (2012)]]
::: '''MITOEAGLE theme'''
::: '''MitoEAGLE theme'''
:::: MITOEAGLE aims at providing the first standardized measures to link mitochondrial and physiome performance (heart rate, BMI, blood pressure, blood chemistry, ''V''<sub>O2max</sub>) to understand the myriad of factors that play a role in mitochondrial physiology. The ambitious objective to optimally harmonize protocols across research groups cannot be achieved through projects with limited numbers of partners. Therefore, the COST framework represents an ideal instrument which allows bringing together many groups and experts in the diverse field of mitochondrial physiology. The aim of this Action is to form a unique well-coordinated network of senior researchers and young investigators, to include well established stakeholders, and to establish a spirit of mentorship and collaboration in contrast to fierce competition which characterized early decades of bioenergetics. The COST Action MITOEAGLE aims at developing a quality management system (QMS), defining common standards for measurements in the field of mitochondrial function.- MITOEAGLE may be seen as too ambitious in the short term, but indispensable at large.
:::: MitoEAGLE aims at providing the first standardized measures to link mitochondrial and physiome performance (heart rate, BMI, blood pressure, blood chemistry, ''V''<sub>O2max</sub>) to understand the myriad of factors that play a role in mitochondrial physiology. The ambitious objective to optimally harmonize protocols across research groups cannot be achieved through projects with limited numbers of partners. Therefore, the COST framework represents an ideal instrument which allows bringing together many groups and experts in the diverse field of mitochondrial physiology. The aim of this Action is to form a unique well-coordinated network of senior researchers and young investigators, to include well established stakeholders, and to establish a spirit of mentorship and collaboration in contrast to fierce competition which characterized early decades of bioenergetics. The COST Action MitoEAGLE aims at developing a quality management system (QMS), defining common standards for measurements in the field of mitochondrial function.- MitoEAGLE may be seen as too ambitious in the short term, but indispensable at large.


::: '''Increase the value and reduce the noise'''
::: '''Increase the value and reduce the noise'''
:::: Enthusiastic and authentic opinion leaders can bring about a change to increase the value and reduce the noise in mitochondrial research specifically, biomedicine at large, and in our society in general. Millions of Euros are wasted on research that is lacking coherent standards. Prespecified and time-stamped protocols are needed, and researchers need to be introduced into adhering to publicly deposited protocols in practice ([[Begley 2015 Circ Res |Begley, Ioannidis 2015]]). MITOEAGLE recommendations will provide practical guidelines for students, scientists and stakeholders.  
:::: Enthusiastic and authentic opinion leaders can bring about a change to increase the value and reduce the noise in mitochondrial research specifically, biomedicine at large, and in our society in general. Millions of Euros are wasted on research that is lacking coherent standards. Prespecified and time-stamped protocols are needed, and researchers need to be introduced into adhering to publicly deposited protocols in practice ([[Begley 2015 Circ Res |Begley, Ioannidis 2015]]). MitoEAGLE recommendations will provide practical guidelines for students, scientists and stakeholders.  
 
::: '''''Further details'': [[MITOEAGLE]]'''
 
 
[[File:SUIT-nomenclature.jpg|right|400px|link= MitoPedia: SUIT|SUIT protocols]]
=== A1: Diagnostic SUIT protocols of mitochondrial fitness ===
::: '''[[MitoPedia: SUIT]]'''
:::: A ‘library of SUIT protocols’ applied in mitochondrial respiratory physiology is collected in a standard format, delineating the diversity of experimental approaches to assess bioenergetic function. A set of [[MiPNet21.06 SUIT-RP |reference protocols]] and validated standard protocols will rigorously document (Maelstrom Research program) the state-of-the-art in designing, conducting, reporting, interpreting, and validating such bioenergetic tests.
<br />
 
 
 
{|style="background:rgb(230,230,230)"
|
 
[[File:SUIT-Catg NFSGpTm.jpg|right|120px]]
: '''09:00-10:15 SUIT protocols: the next steps'''
::: ''Chairs:'' [[Laner V |Verena Laner]], [[Hoppel CL|Charles L Hoppel]]
::::* [[Gnaiger 2014 MitoPathways |'''Gnaiger E''']] (AT) SUIT protocols - MitoPathways 2007-2016.
::::* [[MitoPedia: DatLab |'''Gradl L, Capek O, Plattner C, Droescher S, Doerrier C, Gnaiger E''']] (ORO) '''Release of DatLab 7''' - new dimensions with MitoFit quality control and MitoPedia: DatLab.
::::* [[Doerrier 2016 Abstract MitoFit Science Camp 2016 |'''Doerrier C''', Sumbalová Z, Lamberti G, Krumschnabel G, Gnaiger E]] (ORO) SUIT reference assay for OXPHOS analysis by high-resolution respirometry.
 
: '''10:45-12:00 SUIT protocols: limitations and solutions'''
::: ''Chairs:'' [[Doerrier C|Carolina Doerrier]], [[Kane DA|Daniel Kane]]
::::* [[Sumbalova 2016 Abstract MitoFit Science Camp 2016 |'''Sumbalová Z''', Krumschnabel G, Vančová O, Doerrier C, Gnaiger E]] (ORO) Optimizing strategies on the malate concentration in SUIT protocols.
::::* [[Talk:Fatty acid oxidation |'''Krumschnabel G''']] (ORO) O2k-Network discussion forum: Fatty acid oxidation.
::::* [[Osiki 2016 Abstract MitoFit Science Camp 2016 |'''Osiki PO''', Ojuka E]] (ZA) Suitability of octanoylcarnitine & malate for assessment of beta oxidation capacity by respirometry in aconitase-inhibited samples.
::::* [[Makrecka-Kuka 2016 Abstract MitoFit Science Camp 2016 | '''Makrecka-Kuka M''', Liepinsh E, Volska K, Kuka J, Makarova E, Antone U, Sevostjanovs E, Vilskersts R, Strods A, Tars K, Dambrova M]] (LV) Long-chain acylCoAs vs acylcarnitines in mitochondrial bioenergetics: from ''in vitro'' to ''in vivo''.
|}
 
 
::: '''''Further details'': [[MITOEAGLE protocols, terminology, documentation]]'''
 
 
=== A2: Quality management system for mitochondrial respirometry: the MitoFit proficiency test ===
 
::: '''Quality management system (QMS)'''
:::: The quality of any database is not better than the quality of the primary constituent datasets. Rigorous standards have to be applied to forge information into knowledge. Comparability of data generated by different labs on OXPHOS performance is restricted due to different laboratory protocols applied. Harmonization is clearly required along these lines, including the establishment of mitochondrial reference samples for inter-laboratory proficiency tests, and guidelines for data analysis and reporting (http://www.equator-network.org/).
 
:::: There is no QMS commonly available in the field of mitochondrial respirometry and bioenergetics. Without networking on a large scale it is not possible to implement a widely or even generally accepted set of standards that is required for harmonization of datasets.
 
::: '''Interlaboratory proficiency test'''
[[File:MITOEAGLE Proficiency ring test.jpg|left|400px|MITOEAGLE Proficiency ring test]]
:::: Inter-laboratory ring tests are a basic requirement for quality management. Such proficiency tests require a reference sample which must be homogenous, stable, representative of the diagnostic target, amenable for distribution, and economical for large-scale production. For respiratory OXPHOS analysis a reference sample of structurally and functionally intact mitochondria is not yet available to perform corresponding ring tests (in contrast to enzymatic OXPHOS ring tests). Recently, the widely applied human cell line HEK 293T was found to be potentially suitable for cryopreservation. Upon thawing the cells can be used immediately for respirometry of both intact and permeabilized cells. Thus interlaboratory proficiency testing may now be feasible as a world-wide innovation in the field of mitochondrial respiratory physiology. Participating labs may implement the test for intra- and interlaboratory validation and longitudinal performance monitoring, using a [[SUIT reference protocol]]. Feedback from the participants will lead to adjustments of the SOPs in the proficiency test, which will be made generally available.
 
[[File:SUIT-Reference-Protocol.jpg|right|400px|link=SUIT reference protocol |SUIT reference protocol]]
:::: Comparison of results between and within instrumental platforms: The reference protocols and proficiency test will not define the instrumental platform, but will allow a quantitative comparison of results obtained with specific equipment available in the participating laboratories.
 
::: '''From standardization to harmonization: [[MitoPedia]]'''
:::: The logistics of development of a database requires application of strictly defined terms for all included variables. There is no general reference available upon which a consistent terminology on mitochondrial physiology and bioenergetics can be based. The MITOEAGLE consortium, therefore, has to accomplish the ambitious goal to unify and simplify the terminology in the field for the purpose of the QMS, which will lead to the development of recommendations for the use of a common terminology in mitochondrial physiological research. A publication will be prepared as an Open Access article which will be a milestone towards a unification of concepts and nomenclature.
:::: MITOEAGLE will elaborate definitions of fundamental terms as required by the data management system, including a review on normalization of the data (tissue mass, cell protein, mt-markers such as mt-volume, mt-protein, mt-marker enzymes, flux ratios).
<br />
 
{|style="background:rgb(230,230,230)"
|
: '''15:30-16:45 O2k-demo experiment: reference SUIT protocol with cryopreserved mammalian cells'''
::: ''Chairs:'' [[Votion DM|Dominique-Marie Votion]], [[Ojuka E|Edward Ojuka]]
::::* [[Lamberti 2016 Abstract MitoFit Science Camp 2016 |'''Lamberti G, Krumschnabel G''']] (ORO) Development of a reference sample for HRR.
::::* [[SUIT reference protocol |'''Doerrier C, Lamberti G''', Krumschnabel G, Zumbalová Z, Plattner C, Hiller E, Droescher S, Dikova V, Laner V, Gnaiger E]] (ORO) O2k-Demo experiment: the SUIT reference protocol RP1/RP2.
: '''17:15-18:30 MitoFit proficiency test'''
::::* [[MiPNet21.07 MitoFit DatLab PT |'''Plattner C''', Krumschnabel G, Sumbalova Z, Doerrier C, Gnaiger E]] (ORO) MitoFit DatLab proficiency test.
 
: '''20:30-21:15 A3: Pick up your OROBOROS USB-flash drive with DatLab 7'''
::::* Install DatLab 7 on your laptop.
::::* O2k-users - hands-on: DatLab-proficiency test for flux analysis with SUIT RP1-RP2.
::::* O2k-beginners - hands-on: (1) Introduction to O2-calibration and O2-background. (2) DatLab-proficiency test for flux analysis with SUIT RP1-RP2.
|}
 
::: '''''Further details'' on WG1''':
::::» [[MITOEAGLE protocols, terminology, documentation]]
::::» [[MitoFit proficiency test]]
::::» [[MitoFit Quality Control System]]
 
 
 
=== B1: Mitochondrial assays in blood cells ===
:::: Biopsy sampling of human muscle and fat tissue for mitochondrial studies is an invasive approach. More recently measurement of mitochondrial function in human blood cells – as well as temporary cryopreservation ([[Karabatsiakis 2014 Translational Psychiatry]]) – has become an attractive and by far less invasive alternative for mitochondrial diagnostics. Mitochondrial function of blood cells was linked with numerous diseases and metabolic conditions such as sepsis, neurodegenerative diseases, depression, hypertension, amyotrophic lateral sclerosis, and early-stage heart failure (e.g. [[Sjoevall 2013 Crit Care]]; [[Karabatsiakis 2014 Translational Psychiatry]]; [[Hagl 2014 J Alzheimers Dis]]). The use of human blood cells will thus predictably widen the applicability of respirometry in human physiology and will provide a significant stimulus for the entire field of mitochondrial medicine.
 
:::: A key towards achieving comparability among data sets and the applicability of human blood cells for the evaluation of mitochondrial fitness in health and disease is the standardization of the procedures to separate or isolate blood cells, the experimental procedure for the evaluation of mitochondrial respiratory characteristics, and the format for reporting.
 
:::: Following the [[MitoFit Workshop Blood Cells 2016-01-08]], this will be the '''2nd Workshop on blood cell respirometry for mitochondrial research and diagnostic applications'''.
<br />
{|style="background:rgb(230,230,230)"
|
: '''09:00-10:15 Blood cells for mitochondrial screening'''
::: ''Chairs:'' [[McManus MJ|Meagan McManus]], [[Neufer PD|P Darrell Neufer]]
::::* [[Sumbalova 2016b Abstract MitoFit Science Camp 2016 |'''Sumbalová Z''', Droescher S, Hiller E, Chang S, Garcia L, Calabria E, Volani C, Krumschnabel G, Gnaiger E]] (ORO) Human blood cells: isolation and HRR.
::::* [[Molina AJA |'''Molina AJA''' et al]] (US) Blood-cell bioenergetics and physical function.
::::* [[Karabatsiakis A |'''Karabatsiakis A''' et al]] (DE) Crypreserved PBMCs as a study model of mitochondrial respiration.
::::* [[Irving BA |'''Irving BA''']] (US) PBMC and t-cell mitochondrial function in humans.
::::* Poster: [[Pelnena 2016 Abstract MitoFit Science Camp 2016 |'''Pelnena D''', Lace B, Micule I, Grinfelde I, Inaskina I]] (LV) OXPHOS enzyme activity measurements in mitochondria isolated from peripheral blood leukocytes in control group and patients with suspected mitochondrial disease.
 
: '''10:45-12:00 Workshop: Blood cell preparation, cryopreservation, respiration'''
::: ''Chairs:'' [[Calabria E |Elisa Calabria]], [[Ali SS |Sameh Ali]]
|}
 
::: '''''Further details'' on WG4: [[MITOEAGLE data: blood and cultured cells]]'''
 
 
=== B2: Cell culture models versus tissue samples: - Brain and neuronal cells - Adipose tissue - Other ===
:::: Mitochondrial function in fat tissue has received increasing attention. Adipose tissue exerts not only important endocrine but also metabolic functions. Several studies have investigated mt-capacities in this context, showing that changes in the expression of body fat and adipocytes can be traced to alterations of mt-functional characteristics ([[Yin 2014 J Clin Endocrinol Metab]]). In morbidly obese patients adipocytes from visceral vs subcutaneous fat depots show distinct respiratory control patterns ([[Kraunsoee 2010 J Physiol]]), a finding later confirmed in mice. The great potential of respirometric investigations of fat tissue can be further exploited by developing interlaboratory diagnostic standards.
<br />


{|style="background:rgb(230,230,230)"
::: '''''Further details'': [[MitoEAGLE]]'''
|
: '''15:30-16:45 Cells and tissues'''
::: ''Chairs:'' [[Hsiao CP|Chao-Pin Hsiao]], [[Hickey AJ|Anthony Hickey]]
::::* [[Hoppel 2016c Abstract MitoFit Science Camp 2016 |'''Hoppel CL''']] (US) Mitochondrial respiration in permeabilized skin fibroblasts.
::::* [[Burtscher J 2016 Abstract MitoFit Science Camp 2016 |'''Burtscher J''', Gnaiger E, Schwarzer C]] (AT) Oxidative phosphorylation in the healthy and epileptic mouse brain.
::::* [[Irving BA |'''Irving BA''', Chicco AJ, Garcia-Roves PM]] (US and ES) The rotenone paradox in liver mitochondria.
::::* [[Volani 2016 Abstract MitoFit Science Camp 2016 |'''Volani C''', Demetz E, Doerrier C, Gnaiger E, Weiss G]] (AT) Effects of iron imbalances on mitochondrial activity in mouse liver homogenate and permeabilized rat PBMCs.


: '''17:15-18:30 Tissues and cells'''
::: ''Chairs:'' [[Laner V|Verena L]], [[Irving BA|Brian Irving]]
::::* [[Calzia 2016 Abstract MitoFit Science Camp 2016 |'''Calzia E''', Reichwald K]] (DE) Mitochondrial respiration shows tissue- and strain-specific aging in short- and long-lived ''N. furzeri'' strains.
::::*Poster: [[Herminghaus 2016 Abstract MitoFit Science Camp 2016 |'''Herminghaus A''', Papenbrock H, Eberhardt R, Vollmer C, Schulz J, Truse R, Bauer I, Picker O]] (DE) Sterile laparotomy time-dependently modulates hepatic but not colonic mitochondrial function similar to moderate abdominal sepsis.
::::* [[Madlala 2016 Abstract MitoFit Science Camp 2016 |'''Madlala HP''', Ojuka EO]] (ZA) Fructose-induced defects in selected mitochondrial enzymes are detected by respirometry using glutamate&malate but not pyruvate&malate substrates.
::::* [[Morales-Garcia 2016 Abstract MitoFit Science Camp 2016 |'''Morales-Garcia NL''', Cruz-Cruz I, Uribe-Alvarez C, Chiquete-Felix N, Uribe-Carvajal S]] (MX) Respiratory deficiencies in isolated mitochondria from a Δ-shy1 ''S. cerevisiae'' strain.
::::* [[Uribe-Alvarez 2016 Abstract MitoFit Science Camp 2016 |'''Uribe-Alvarez C''', Chiquete-Felix N, Contreras-Zentella M, Guerrero-Castillo S, Pena A, Uribe-Carvajal S]] (MX) In ''Staphylococcus epidermidis'', oxygen variations promote differential expression of respiratory enzymes that constitute possible therapeutic targets.
|}


::: '''''Further details'' on WG3: [[MITOEAGLE data: fat]]'''
:::» '''For the programme in detail please see: [[MitoFit_SC_2016_Programme information]]'''  




=== B3: Mitochondrial studies in neurodegeneration ===
== MitoEAGLE: towards the 1<sup>st</sup> MC Meeting ==


{|style="background:rgb(230,230,230)"
:::* List of MC Members: http://www.cost.eu/COST_Actions/ca/CA15203?management
|
: '''20:30-21:15'''
::: ''Chairs:'' [[Bezuidenhout N|Nicole Bezuidenhout]], [[Marcinek DJ|David Marcinek]]
::::* [[McManus 2016 Abstract MitoFit Science Camp 2016 |'''McManus MJ''']] (US) A multi-tiered mitochondrial approach to predictive biomarkers of neurodegenerative disease.
::::* [[Schaefer 2016 Abstract MitoFit Science Camp 2016 |'''Schaefer PM''', von Einem B, Walther P, Calzia E, Rueck A, von Arnim CAF]] (DE) Prominent role of amyloid beta and its intracellular localization in Alzheimer´s disease associated mitochondrial dysfunction.
::::* [[Verebne Tar 2016 Abstract MitoFit Science Camp 2016 | Czinege R, Yazaki R, Virag L, '''Verebne Tar K''']] (HU) Investigating the role of the proteasome activator PA200 in mitochondrial homeostasis in a cellular model for Huntington’s disease.
::::* [[Krako 2016 Abstract MitoFit Science Camp 2016 |'''Krako N''', Meli G, Manca A, Magnifico MC, Arese M, Sarti P, Cattaneo A]] (RS) Conformational targeting of amyloid β oligomers inside endoplasmic reticulum rescues mitochondrial deficits in 7PA2 cells.
::::* Poster: [[Hsiao 2016 Abstract MitoFit Science Camp 2016 |'''Hsiao CP''', Daly B, Hoppel CL]] (US) Association between mitochondrial bioenergetics and radiation-related fatigue: a possible mechanism and novel target.  
|}


=== C1: Skeletal and cardiac muscle fibres - towards a data repository. ===
:::: The sessions on the [[COST Action MitoEAGLE]] at the MitoFit Science Camp provided an excellent opportunity to discuss some key topics in preparation of the 1<sup>st</sup> Management Committee (MC) Meeting, including several Management Committee Members who participated in the full MitoFit SC or joined specifically for this discussion.
:::: During the past 10 years, a dramatically increasing number of studies report respirometric data on human muscle tissue ([[O2k-Publications: Skeletal muscle]]. However, despite the many published data, lack of consistency often precludes quantitative comparison among data sets with permeabilized or homogenized muscle fibres or isolated mitochondria, normalized to either tissue wet weight, dry weight, protein content, citrate synthase activity or other [[mitochondrial marker]]s. In the absence of validated conversion factors, these data remain largely unconnected and can only be qualitatively linked.


:::: The aim will be to provide access to complete data sets for post-study statistical analysis and use harmonization tools for standardized documentation, to initiate a data repository on muscle tissue from humans and model organisms in health and disease. Data sets shall be obtained from already published and new studies, prompting participating labs to report experimental details beyond those already published as far as possible (using tools such as PubMed Commons). Specific protocols are presented and compared for handling muscle tissue.
:::: The [[MitoEAGLE#Working_Groups |Working Groups]] (WG) were divided into '''Task Groups'''. This was particularly important for the large WG1 (Standard operating procedures and user requirement document: Protocols, terminology, documentation). The tentative WG5 (Dissemination and training) could thereby be incorporated into WG1.
<br />


{|style="background:rgb(230,230,230)"
|
: '''09:00-10:15 Quantitative studies'''
::: ''Chairs:'' [[Makrecka-Kuka M|Marina Makrecka-Kuka]], [[Molina AJA|Anthony JA Molina]]
::::* [[Hoppel 2016a Abstract MitoFit Science Camp 2016 |'''Hoppel CL''']] (US) Skeletal muscle mitochondria in diagnostic studies.
::::* [[Garcia-Roves 2016b Abstract MitoFit Science Camp 2016 |Gama-Perez P, Moreno S, Gonzalez-Franquesa A, '''Garcia-Roves PM''']] (ES) Technical perspective of high-resolution respirometry in permeabilized skinned muscle fibers from different mouse models.
::::* [[Coen PM |'''Coen PM''', Goodpaster BH]] (US) Improved mitochondrial function in human skeletal muscle - quantification.
::::* [[Neufer 2014 Abstract MiP2014 |Lark DS, Torres MJ, Lin CT, Ryan TE, Anderson EJ, '''Neufer PD''']] (US) Direct real-time quantification of mitochondrial oxidative phosphorylation efficiency in permeabilized skeletal muscle myofibers.
::::* [[Marcinek 2016 Abstract MitoFit Science Camp 2016|'''Marcinek DJ''', Campbell MD, Siegel MP, Kruse SA, Kramer PA]] (US) Targeting mitochondrial redox stress reveals two phases for reversal of mitochondrial dysfunction in aged mouse skeletal muscle.


: '''10:45-12:00 Oxygen dependence of respiration in permeabilized fibres: respiration media, inhibitors, sample preparation'''
::: <big>'''Chair, Vicechair and Steering Committee '''</big>
::: ''Chairs:'' [[Sumbalova Z|Zuzana Sumbalova]], [[Coen P|Paul Coen]]
::::* Merth A, Droescher S, Bezuidenhout N, Doerrier C, Gradl P, Gnaiger E (WGT and ORO) A DatLab-controlled cooling plate for permeabilized muscle fibre preparation.
::::* [[Bezuidenhout 2016a Abstract MitoFit Science Camp 2016| '''Bezuidenhout N''', Droescher S, Doerrier C, Gnaiger E]] (ORO) Comparison of oxygen dependence of respiration in permeabilized mouse skeletal muscle fibers in two respiration media, MiR06Cr and Buffer Z containing Ctl, Cr and Blebbistatin.
::::* [[Kane 2016 Abstract MitoFit Science Camp 2016 | '''Kane DA''']] (CA) Effects of inhibiting myosin-ATPase on mitochondrial respiratory capacity in permeabilized muscle.
::::* [[Chicco 2016a Abstract MitoFit Science Camp 2016 |'''Chicco AJ''', Hedges MR, Rasicci DV, Specht KS]] (US) Oxygen dependence of H<sub>2</sub>O<sub>2</sub> release from skeletal muscle and cardiac mitochondria: influence of sample preparation.
|}


::: '''''Further details'''''
:::: All suggestions may be turned over at the 1st Management Committee Meeting.
::::» [[MITOEAGLE data repository in muscle tissues]]
::::» WG2: [[MITOEAGLE data: muscle]]


:::: Among the participants, assuming that the Main Proposer ([[Gnaiger E|Erich Gnaiger]]) will be nominated as Chair of the COST Action MitoEAGLE, [[Calabria E|Elisa Calabria]] was suggested as the Vicechair to be nominated at the 1<sup>st</sup> MC Meeting.


[[File:MITOEAGLE Working groups.jpg|right|400px|MITOEAGLE Working Groups]]
::: '''Steering Committee'''
=== D1: Towards a database on mt-respiratory physiology: from laboratory standards to inter-laboratory harmonization ===
:::: A Steering Committee should be nominated, consisting of the Chair, Vicechair, WG leaders, regional representatives (not MC members) beyond the limits of COST countries, a Financial Officer ([[Keijer J|Jaap Keijer]] should be consulted for suggestions), and a General Secretary ([[Plattner C|Christina Plattner]]).
:::: Adherence to common standards is likely to increase the proportion of true findings (Ioannidis 2005; Fortier et al 2010). A data bank with harmonized datasets will advance significantly the field of human and comparative bioenergetics research and provide the level of knowledge required for helping health systems to evolve.


:::: The complexity of evolutionary background, age, gender, lifestyle and environment (EAGLE) linked to mitochondrial function cannot be addressed in the conventional small-scale studies with 20 subjects (rather than 2,000 or 200,000). To come anywhere near to large sample sizes, pooling of information between studies is essential on a global scale.
:::: The following criteria should be kept in mind for nominations: (1) Expertise; (2) Regions - add non-MC members as links to other continents; (3) Gender balance; (4) participation of Early Career Investigators (researchers in the time span of up to 8 years after the date of obtaining the PhD/doctorate). Among the participants, the following WG leaders were suggested, and a co-leader should be additionally nominated for the 1<sup>st</sup> MC Meeting:
 
:::: Scientists may refute the challenge of comparing, optimizing and even pre-registering their protocols, when employment and assessment procedures exert stronger pressures on unhealthy competition by high-profile publication than on time-consuming quality control by collaboration. Young investigators may lack a broad perspective on methodological standards and may be reluctant to deviate from a trotten path. MITOEAGLE, however, will bring a large group of mitochondrial experts together by demonstrating the added value for each individual project, when harmonization of protocols will allow scattered data to be combined as the only means to overcome severe limitations of the traditional scientific approach ([[Fortier 2010 Int J Epidemiol]]).
 
:::: Rigorous standardization is the gold standard for data pooling, but may not be achievable at the present level of research in mt-physiology, where different preparation protocols, incubation media and respiratory protocols are used. Therefore, unrealistic efforts towards developing and implementing a defined standard will be replaced by a translation strategy: Major subprotocols (e.g. different respiration media) will be compared (facilitated by STSM and workshops) to allow quantitative conversion of results obtained by different groups following their specific ‘standard’ operating procedures. This will be a first evolutionary step towards harmonization and standardization.
 
:::: An ‘absolute‘ gold standard may remain inherently unattainable, as is the case in most fields of contemporary bioscience. Practically, the least common denominator that can be agreed upon may remain disappointingly fragmented, particularly in a large and diverse consortium involving stakeholders represented by university-based and industry-based scientists. Senior stakeholders may be biased by a commitment to their established  - scientifically published or commercially sold - procedural protocols, which appear to be ‘accepted’ (by peer review) or ‘accredited’ (by revenues).
 
::::» Compare: Mitochondrial disease sequence data resource - https://mseqdr.org/
<br />
 
{|style="background:rgb(230,230,230)"
|
: '''09:00-10:15 Data base and harmonization studies'''
::: ''Chairs:'' [[Plattner C|Christina Plattner]], [[Chicco A|Adam Chicco]]
::::* [[Garcia-Roves 2016a Abstract MitoFit Science Camp 2016 |Gonzalez-Franquesa A, Gama-Perez P, Aguilar A, Yanes O, Martin-Subero JI, Sales-Pardo M, Guimera R, '''Garcia-Roves PM''']] (ES) Design and implementation of systems biology approaches to integrate heterogenic data in biomedical research.
::::* [[Drinnan 2016 Abstract MitoFit Science Camp 2016 |'''Drinnan M]]''' (UK) The O2k in 2020: Letting Datlab outside its box.
::::* '''[[Molina AJA]]''' (US) UPBEAT project, database and self-learning software.
::::* [[Krumschnabel 2016b Abstract MitoFit Science Camp 2016 | '''Krumschnabel G''', Hiller E, Gnaiger E]] (ORO) O2k-MultiSensor: Mitochondrial respiration media for HRR and simultaneous O2k-Fluorometry.
::::* [[Hoppel 2016b Abstract MitoFit Science Camp 2016 |'''Hoppel CL''', Gnaiger E]] (US) Comparison of permeabilized skeletal muscle fibers or isolated mitochondria for the detection of oxidative phosphorylation defects.
::::*Poster: [[Markova 2016 Abstract MitoFit Science Camp 2016 |'''Markova M''', Cedíkova M, Grundmanova M, Kuncova J]] (CZ) Porcine hearts samples preparation: PBI-Shredder or saponin?
::::* [[Krumschnabel 2016a Abstract MitoFit Science Camp 2016 | Doerrier C, Eigentler A, Krautschneider M, Schoepf B, Fontana-Ayoub M, Gnaiger E, '''Krumschnabel G''']] (ORO) O2k-Protocols: Mitochondrial preparations for HRR.
::::* [[Irving BA |'''Irving BA''']] (US) Shredder: lessons learned.
 
: '''10:45-12:00 MITOEAGLE nomenclature - roundtable'''
::: ''Chairs:'' [[Lamberti G|Giorgia Lamberti]], [[Garcia-Roves PM|Pablo Miguel Garcia-Roves]]
::::* '''Gnaiger E''', '''Krumschnabel G''' (ORO) Introduction: Do bioenergetics and mitochondrial physiology need a consistent nomenclature to become MitoFit? - Expand '[[MitoPedia]]' as a basis for a joint publication on 'concepts and nomenclature'?
|}
:::: [[MITOEAGLE protocols, terminology, documentation |Harmonization of nomenclature on mitochondrial respiratory states and control parameters]]: The logistics of development of a database requires application of strictly defined terms for all included variables. There is no general reference available upon which a consistent terminology on mitochondrial physiology and bioenergetics can be based. The MITOEAGLE consortium, therefore, has to accomplish the ambitious goal to unify and simplify the terminology in the field for the purpose of the QMS, which will lead to the development of recommendations for the use of a common terminology in mitochondrial physiological research. A publication will be prepared as an Open Access article which will be a milestone towards a unification of concepts and nomenclature.
 
 
=== D2: Mitochondrial health: molecules, cells and tissues ===
:::: Diseases that are strongly related to a sedentary life style are spreading world-wide at an epidemic scale. Mitochondrial dysfunction is increasingly associated with the progression of such pathologies: cause or consequence? There is currently no regimented, quantitative system, or database organised to routinely test, compare and monitor mitochondrial capacities within individuals, populations, or among populations. This reflects the need for scientific innovation and represents a shortcoming in the health system of our modern, rapidly ageing society. Traditional lifestyles of ‘remote’ human populations are dwindling such that these highly significant windows into our evolutionary past are about to becoming closed, forever. While studies of mitochondrial DNA (mtDNA) have become increasingly established in the search for the evolutionary history and diversification of the human species, mitochondrial function remains comparatively unexplored, but may turn out to provide key information for preventing diseases which become prevalent upon transition to a Westernized lifestyle. The presently observed acceleration of research on cell and mitochondrial respiration represents a challenge to transform scientific information into knowledge and translate complex results on mitochondrial (dys)function into a patient-related mitochondrial score. Diagnostic standards, however, are lacking. A concerted action of the scientific community is required to implement quality control systems and optimally harmonize protocols across research groups. The primary challenge is to ‘increase value and reduce waste in research design, conduct, and analysis’, to separate the signal from the noise ([[Ioannidis 2005 PLoS Med]]; The Lanzet 2014): ‘The signal is the truth. The noise is what distracts us from the truth’ ([[Silver 2012 Penguin Press |Silver N: The signal and the noise. The art and science of prediction. Penguin Press 2012]]).
<br />
 
{|style="background:rgb(230,230,230)"
|
: '''15:30-16:45 Exercise at high altitute and high performance'''
::: ''Chairs:'' [[Renner-Sattler K|Kathrin Renner-Sattler]], [[Goodpaster BH|Bret Goodpaster]]
::::* [[Burtscher M 2016 Abstract MitoFit Science Camp 2016 |'''Burtscher M''']] (AT) Living and exercising at moderate altitudes: health risk or benefit?
::::* [[Chicco 2016b Abstract MitoFit Science Camp 2016 |'''Chicco AJ''', Le CH, Gnaiger E, Dreyer HC, Hocker AD, Lovering AT, Subudhi AW, Roach RC]] (US and AT) OXPHOS coupling efficiency of permeabilized muscle fibers predicts metabolic efficiency of subjects exercising at 5260 m.
::::* [[Votion 2016 Abstract MitoFit Science Camp 2016 |'''Votion DM''', Leleu C, Robert C, Serteyn D]] (BE) OXPHOS capacities correlate with racing performance and indicate risk of developing exercise-induced myopathy.
::::* [[Gnaiger 2016 Abstract MitoFit Science Camp_2016 |'''Gnaiger E''', '''Laner V''', Hamilton KL, Miller BF, Boushel RC, Buser M, Williamson KK, Davis MS]] (ORO, CA and US) OXPHOS and ETS capacity in permeabilized fibres of canine superathletes. Analysis of HRR data presenting unexpected challenges.
 
: '''17:15-18:30 Mitochondrial function and dysfunction'''
::: ''Chairs:'' [[Herminghaus A|Anna Herminghaus]], [[Calzia E|Enrico Calzia]]
::::* [[Hickey 2016 Abstract MitoFit Science Camp 2016 |Mason S, Devaux J, Hedges C, '''Hickey AJ''']] (NZ) How do bumblebees warm flight muscles?
::::* [[Krajcova 2016 Abstract MitoFit Science Camp 2016 |'''Krajcova A''', Waldauf P, Andel M, Duska F]] (CZ) Mitochondrial pathogenesis of propofol infusion syndrome in an ''in vitro'' model of human skeletal muscle.
::::* [[Chicco 2016c Abstract MitoFit Science Camp 2016 |'''Chicco AJ''', Le CH, Benage LG, Prenni JE, Heuberger AL]] (US) Investigating the mechanism of cardiac mitochondrial respiratory impairment in Barth Syndrome.
::::*Poster: [[Pillegi 2016 Abstract MitoFit Science Camp 2016 |'''Pileggi C''', Hedges CP, Vickers MH, McGlashan SR, Hickey AJR, Cameron-Smith D, Firth EC, Gray C]] (NZ) Voluntary exercise prevents high fat diet-induced cardiac mitochondrial dysfunction in male rats.
::::* [[Maarman 2016 Abstract MitoFit Science Camp 2016 |'''Maarman G''', Andrew BM, Blackhurst D, Ojuka EO]] (ZA) Melatonin rescues uric acid-induced impairment of respiration in myotubes.
|}
 
=== D3: MoTrPAC and MITOEAGLE  ===
::: '''The molecular transducers of physical activity consortium (MoTrPAC) and MITOEAGLE: exercise effects on mitochondria'''
<br />
{|style="background:rgb(230,230,230)"
|
:'''20:30-21:15'''
::: ''Chairs:'' [[Renner-Sattler K|Kathrin Renner-Sattler]], [[Thierry A|Arnould Thierry]]
::::» [[Goodpaster 2016 Abstract MitoFit Science Camp 2016 |Abstract by Bret H Goodpaster]] (US)
:::: The MoTrPAC is a new initiative funded by the U.S. National Institutes of Health to investigate the biological molecules in response to acute and chronic exercise, and to relate these changes to the benefits of physical activity. This molecular map will contain the many molecular signals that transmit the health effects of physical activity, and indicate how they are altered by age, sex, and fitness level. Clinical centers across the U.S. will obtain skeletal muscle, adipose tissue and blood specimens in ~2,500 healthy human subjects before and after bouts of acute exercise and following an exercise training program, and in a smaller number of non-exercise control subjects and in highly-trained athletes. Although not likely to be included in the primary analyses of blood and tissue specimens, mitochondria was a featured topic of importance during the NIH-convened working group webinars and discussions that helped guide the MoTrPAC. We will propose an ancillary study to investigate the effects of exercise on mitochondrial genetics, energetics, dynamics, e.g., fusion, fission, mitophagy, epigenomics, oxidative stress and redox biology in skeletal muscle, adipose tissue and peripheral blood cells. This project within the MoTrPAC could synergize with MITOEAGLE to provide novel insights about the role of mitochondria and exercise in human health.
 
[[File:MITOEAGLE-representation.jpg|right|400px|link=http://www.mitoglobal.org/index.php/MITOEAGLE|COST Action MITOEAGLE]]
::: '''Mitochondrial physiology:''' '''E'''volution, '''A'''ge, '''G'''ender, '''L'''ifestyle and '''E'''nvironment
:::: The objective of the '''[[MITOEAGLE]]''' network is to improve our knowledge on mitochondrial function in health and disease related to '''E'''volution, '''A'''ge, '''G'''ender, '''L'''ifestyle and '''E'''nvironment. Every study of mitochondrial (mt) function and disease is faced with EAGLE as the essential background conditions characterizing the individual patient, subject, study group, species, tissue or even cell line. To address the complex interrelationships of EAGLE with an initial focus on humans and rodent models, the network will enhance the value of each individual study by starting to analyse and catalog data beyond the published record. Highlighting the topic of gender and mitochondrial function, unique new information will emerge on human biology from the development of a European reference database. Protocols, technologies and standard procedures will be compared and strategies defined for improvement of quality control. An inter-laboratory ring test will be established as a world-wide innovation in the field of mitochondrial respiratory physiology. The expertise gained and new standards developed will be integrated into a strategic dissemination and education programme for mitochondrial phenotyping, aiming at an expanding European and MitoGlobal EAGLE network where researchers collaborate on mapping mitochondrial physiology and medicine, complementary to established mtDNA databases.
 
:::: Only a large and '''well coordinated network''' can manage to generate the necessary number of consistent data to address the complexity of EAGLE, since the range of factors is too wide to be accommodated in any single project on mitochondrial respiration. The MITOEAGLE knowledge management network will be a strategic innovation to develop harmonization protocols towards generating a rigorously monitored data repository on mitochondrial respiratory function. A '''MITOEAGLE data management system''' is necessary to interrelate results of a large number of studies, to interpret pathological phenotypes, and to set results into the multidimensional context of EAGLE. Clearly, MITOEAGLE must be operated by mitochondrial specialists on a global scale. MITOEAGLE will be a gateway and milestone to better diagnose mitochondrial respiratory defects which are linked to various age-related health risks, including cardiovascular and degenerative diseases, such as type 2 diabetes, neurodegenerative diseases (Alzheimer’s, Parkinson’s, Huntington’s), and several types of cancer ([[Murdoch 2013 JAMA]]).
|}
 
[[Image:Gentle-Science_Ganesha.jpg|right|110px|link=http://www.bioblast.at/index.php/Gentle_Science|Gentle Science]]
=== E1: MITOEAGLE and parallel IOC112 O2k-Workshop  ===
 
{|style="background:rgb(230,230,230)"
|
: '''09:00-10:15'''
::: ''Chairs:'' [[Weidinger A|Adelheid Weidinger]], [[Pesta D|Dominik Pesta]]
::::* [[Goebel G |Georg Göbel]] (AT) A supervised statistical approach: about the meaning of the mean and beyond in small sample sizes.
 
::::* The [[MITOEAGLE network]].
::::* IOC112 O2k-Workshop - experimental working groups.
 
: '''10:45-12:00'''
:::: ''continued''
|}
 
=== E2: MITOEAGLE and parallel IOC112 O2k-Workshop ===
<br />
{|style="background:rgb(230,230,230)"
|
: '''15:30-16:45'''
::::* MITOEAGLE: towards the [[COST Action MITOEAGLE |1<sup>st</sup> MC Meeting]].
::::* IOC112 O2k-Workshop - experimental working groups.
 
: '''17:15-18:30'''
:::: ''continued''
|}


:::[[Image:Logo OROBOROS INSTRUMENTS.jpg|40px|link=OROBOROS INSTRUMENTS|OROBOROS]]* IOC112 - '''[[O2k-Workshop]]'''.
::: '''WG leaders'''
::::::* 2016-09-06: Erich is attending the '''COST Preparatory Workshop for Main Proposers''' at the COST Office in Brussels. Here are some relevent suggestions from COST:
::::::::* WG leaders have to be MC members, exceptionally MC substitute.
::::::::* Colleagues from '''Inclusiveness Target Countries (ITC)''' and '''Early Career Investigator (ECI)''' should be well represented as WG leaders, and rotation of some MC members for ECI should be considered by some COST National Coordinators.
::::::::* A rotational system can be implemented and is even encouraged. WG co-leaders may be elected at the MC meeting with the intention to rotate at a given time point between WG leader  and WG co-leader. Both should be MC members.
::::::::* Define in advance '''Short Term Scientific Missions''' with ITC and ECI in mind, decide on assessment criteria.




== Registration, travel information and support ==
::::* WG1: [[Renner-Sattler K| Kathrin Renner-Sattler]]
::::* WG2: [[Makrecka-Kuka M| Marina Makrecka-Kuka]]
::::* WG3: [[Borutaite V| Vilma Borutaite]] - (not present)
::::* WG4: [[Calzia E| Enrico Calzia]] (but: Enrico is not a MC member - Enrico is of course welcome as a WG participant)
::::* WG5: will be incorporated into WG1
 
::: '''International partner representatives'''
::::* [[Goodpaster BH| Bret H Goodpaster]] (North America)
::::* [[Hickey AJ| Anthony J Hickey]] (Australasia)
::::* [[Vercesi A| Anibal Vercesi]] (South America) - (not present)
::::* [[Ojuka EO| Edward O Ojuka]] (Africa) - (not present: [[Ali SS| Sameh Ali]])
::::* Central Asia
::::* Southeast Asia


::::» [[MitoFit_SC_2016_Travel_info | Travel information]]
::::» [[MitoFit_SC_2016_Registration|Registration and cancellation policy]]


::: <big>'''Working groups with a list of task forces'''</big>


:::: '''Scholarships:'''
::: '''WG1: [[MitoEAGLE protocols, terminology, documentation]]'''
:::: OROBOROS INSTRUMENTS will support students with scholarships of EUR 200.-/person. Up to 25 scholarships will be granted.
::::* Coupling states and coupling control ratios: Consensus statements. – [[Renner-Sattler K| Kathrin Renner-Sattler]] / [[Gnaiger E| Erich Gnaiger]] / [[Hoppel CL| Charles L Hoppel]]
:::: Send us your abstract in order to apply for a scholarship. The abstract will then be evaluated by the MitoFit Scientific Committee.
::::* SUIT protocols: nomenclature consistent with concept of database. [[Gnaiger E| Erich Gnaiger]] / ...
::::* Experimental details in SUIT. [[Chicco AJ| Adam Chicco]] / [[Hickey AJ| Anthony J Hickey]] / [[Doerrier C| Carolina Doerrier-Velasco]]
::::* Instrumental platforms: comparisons. – [[Molina AJA|Anthony J Molina]] / (not present: Carlos Canto / Antonio Zorzano / Karl Johan Tronstad)
::::* Knowledge management system. – [[Drinnan M| Michael Drinnan]] / [[Plattner C| Christina Plattner]]
::::* Proficiency test: [[Gnaiger E| Erich Gnaiger]] / [[Doerrier C| Carolina Doerrier-Velasco]]
::::* Documentation: [[Arnould T| Thierry Arnould]] / [[Votion DM| Dominique-Marie Votion]]
::::* Dissemination Scientific community: [[Gnaiger E| Erich Gnaiger]] / [[Makrecka-Kuka M| Marina Makrecka-Kuka]]
::::* Translation to community in general: [[Irving BA| Brian A Irving]] / ...
::::* Training, Short Term Scientific Missions: (not present: [[Klaus S| Susanne Klaus]])


::: '''WG2: [[MitoEAGLE data: muscle]]'''
::::* Skeletal: [[Garcia-Roves PM| Pablo M Garcia-Roves ]] / [[Votion DM| Dominique-Marie Votion]] / [[Coen P|Paul Coen]]
::::* Cardiac: [[Larsen TS| Terje S Larsen]] / [[Makrecka-Kuka M| Marina Makrecka-Kuka]]


::::'''Contact:''' [[Laner V |Laner Verena]], OROBOROS INSTRUMENTS, MitoFit project manager
::: '''WG3: [[MitoEAGLE data: fat |MitoEAGLE data: tissues]]'''
::::* Fat: (not present: [[Nedergaard J|Jan Nedergard]])
::::* Liver: (not present: [[Cervinkova Z|Zuzana Cervinkova]]) / [[Sumbalova Z| Zuzana Sumbalova]]
::::* Neuronal: (not present: [[Sandi C|Carmen Sandi]] / [[Borutaite V|Vilma Borutaite]])


::: '''WG4: [[MitoEAGLE data: blood and cultured cells]]'''
::::* Blood: [[Calabria E| Elisa Calabria]] / [[Calzia E| Enrico Calzia]]
::::* Cultured cells: [[Renner-Sattler K| Kathrin Renner-Sattler]] / ...


::: '''Partners'''
::: '''Partners'''
[[Image:Logo MitoFit_s.jpg|80px|link=http://www.mitofit.org/index.php/MitoFit|MitoFit]] [[File:MUI Logo rz rgb.jpg|80px]] [[File:LFU-Innsbruck-logo.png|40px]] [[Image:Logo OROBOROS INSTRUMENTS.jpg|80px|link=OROBOROS INSTRUMENTS|OROBOROS]] [[File:WGT Logo 2012.jpg |80px |link=http://www.wgt.at|WGT]]  [[File:Logo_HLZ-Kuehtai.png |110px|link=http://www.hoehentraining-kuehtai.at/|HLZ Kuehtai]] [[File:Logo_Sporttherapie-Huber.png |100px|link=http://www.sporttherapie-huber.at|Sporttherapie Huber]] [[File:Tirol Logo Standortagentur.jpg|80px]]
[[Image:Logo MitoFit_s.jpg|80px|link=http://www.mitofit.org/index.php/MitoFit|MitoFit]] [[File:MUI Logo rz rgb.jpg|80px]] [[File:LFU-Innsbruck-logo.png|40px]] [[Image:Logo OROBOROS INSTRUMENTS.jpg|80px|link=Oroboros Instruments|Oroboros ]] [[File:WGT Logo 2012.jpg |80px |link=http://www.wgt.at|WGT]]  [[File:Logo_HLZ-Kuehtai.png |110px|link=http://www.hoehentraining-kuehtai.at/|HLZ Kuehtai]] [[File:Logo_Sporttherapie-Huber.png |100px|link=http://www.sporttherapie-huber.at|Sporttherapie Huber]] [[File:Tirol Logo Standortagentur.jpg|80px]]
<br />
<br />
<br />
<br />


::: '''Additional support'''


[[File:Exerkine.JPG|200px]]
== Additional support ==
:::: [[File:Stealth-logo-full.png|250px|Stealth Biotherapeutics]]
:::: [[Image:Logo OROBOROS INSTRUMENTS.jpg|80px|link=Oroboros Instruments|Oroboros ]]  


[[Image:MitoGlobal.jpg|right|80px|link=MitoGlobal|MitoGlobal]]  
[[Image:MitoGlobal.jpg|right|80px|link=MitoGlobal|MitoGlobal]]  
  Listed under [[MitoGlobal Events]].
  Listed under [[MitoGlobal Events]].

Latest revision as of 13:27, 3 April 2022

Bioblast 2022



MitoFit Science Camp 2016 Kuehtai AT

Publications in the MiPMap
MitoFit

Kuehtai AT, 2016 Jul 07-13. MitoFit Science Camp and IOC112.

» Bioblast pdf

Oroboros (2016-07-07) MitoGlobal

Abstract: 2016 Jul 07-13, Kuehtai, AT. The project MitoFit aims at developing novel laboratory standards and diagnostic monitoring of a mitochondrial fitness score. With an international team of outstanding mitochondrial experts, the MitoFit Science Camp will provide a unique opportunity to receive first-hand introductions to state-of-the-art diagnostic monitoring of mitochondrial respiratory function combined with hands-on training on HRR (IOC112). Diverse areas are covered such as protective medicine, exercise physiology, mitochondrial pharmacology, aging, and comparative mitochondrial physiology (cell types, tissues, species). The common focus is on methodology, experimental details and quality control. The MitoFit Science Camp is a student- and research-oriented even, and may be considered as a sequence of workshops rather than a conference or school, addressing key topics of the COST Action MitoEAGLE project. This will provide a key opportunity to prepare the 1st Management Committee Meeting.


• O2k-Network Lab: AT Innsbruck Oroboros


Labels: MiParea: mt-Awareness 






2016, ORO, O2k-Network, MitoFit 


MitoFit
Johannes Burtscher, Patrick Schaefer, Luiz Felipe Garcia e Souza, Pablo Garcia-Roves, Enrico Calzia, Stephen Dozier, Ignazio Prieto, Verena Laner, Erich Gnaiger, Devin Manning, Pedro Neves
MitoFit Science Camp - for and with mitochondrial scientists


Date, venue, sports programme

Date

2016, July 07-13
HLZ Kuehtai
Immediately following EBEC2016 (July 2-7) organized by Paolo Bernardi in Riva del Garda (2.5 hours from Innsbruck).

Venue

High Altitude Training Center Kühtai, Kuehtai (Tyrol, Austria), 2020 m a.s.l. (42 min from Innsbruck).


Scientific sessions

Sporthotel Kühtai
A-6183 Kühtai • Nr. 9
www.sporthotel-kuehtai.com

Accommodation, breakfast and dinner

Hotel Astoria
A-6183 Kühtai • Nr. 33
Tel.: +43 5239-5215 • Fax: +43 5239-5215-80 • Mail [email protected]
www.hotelastoria.at
  • Additional accomodation: sourrounding apartments/hotels.

Sports programme

  • Activities at the participant's own risk. Sport programme is offered in collaboration with the Institute of Sport Science (University Innsbruck), Sporttherapie Huber, and the High Altitude Training Centre Kühtai.
  • Sign in as a subject: spiroergometric tests (limited number of participants).
  • MitoFit: expertise by experience - high altitude sports and physiotherapy in the Kuehtai environment.
HLZ Kuehtai » http://www.hoehentraining-kuehtai.at/
Sporttherapie Huber » http://www.sporttherapie-huber.at
» Meet the MitoFit athlete: a unique training experience
» Meet the MitoFit expert: groups and one-on-one sessions
» Walks&Talks


Feedback

  • First I want to congratulate you for an excellent meeting and a great opportunity to start organizing the eCOST MitoEAGLE. - Pablo M Garcia-Roves, O2k-Network Lab: ES Barcelona Garcia-Roves PM
  • Participating at the workshop was very interesting and I enjoyed it a lot. Thank you for the perfect organisation. - Carmina Silaidos, O2k-Network Lab: DE Frankfurt Eckert GP
  • I want to say thank you to the whole Oroboros Team, and of course especially to Erich!! - Chiara Volani
  • Congratulations again to a great meeting - a successful approach to 'targeting, body & mind' - Dominik Pesta, O2k-Network Lab: DE Duesseldorf Roden M
  • Thank you very much for the well organised and very interesting MitoFit Science Camp. I am really happy that I could take part in it! - Dita Pelnena
  • Thanks so much! Really appreciate it. Thank you for the opportunity to present. Was a great meeting! - Nicole Bezuidenhout, O2k-Network Lab: ZA Cape Town Ojuka EO
  • Congratulations to you and the Oroboros crew for an interesting and spirited MitoFit Science Camp! - Daniel Kane, O2k-Network Lab: CA Antigonish Kane DA
  • Thank you for an awesome conference, it was a delight and honour to have presented my work! - Prisca Osiki, O2k-Network Lab: ZA Cape Town Ojuka EO
  • The workshop was great! I had a lot of fun and everything was very organized. - Chantal Pileggi, O2k-Network Lab: NZ Auckland Hickey AJ
  • I thank you very much for this outstanding, perfectly organized science camp. It was a great opportunity for me to participate in order to start networking with "mitochondria people", getting to know the Oxygraph, methods, protocols. I am well certain that soon I will be also a user of the Oroboros instrument as well. - Krisztina Verébné Tar
  • Again thank you for the very nice organisation of this meeting. Everything was perfect scientifically. - Thierry Arnould
  • The meeting was fantastic as always! - Charles L Hoppel, O2k-Network Lab: US OH Cleveland Hoppel CL


Impressions from the MitoFit Science Camp 2016

(c) Erich Gnaiger and Verena Laner, if not stated otherwise in the additional information to the specific picture.

  • The first MitoFit Science Camp in Kuehtai with nearly 60 participants is going to start.

  • Erich Gnaiger giving a lecture on the O2k and the latest scientific results.

  • Carolina Doerrier, Oroboros Instruments, preparing chemicals for the demo experiment with reference protocol 1 & 2.

  • Some very diligent and hardworking participants - doing the DL7 proficiency test.

  • Erich Gnaiger introducing the beginners to DatLab7 in an evening session.

  • Having lots of fun with a volleyball tournament - a very pleasant activity.

  • All participants in action, taking the tournament very seriously.

  • All playing with full physical implication and high enthusiasm.

  • Concentration during service.

  • Stephen Dozier full in action.

  • Michaela Markova is highly delighted, Verena Laner in the background.

  • Team meeting on the sports ground and discussing the strategy.

  • Other participants relaxing by playing football - taking part in their own UEFA European Championship.

  • Let a new day start - Yoga-morning-session with Nicole Bezuidenhout on the Hotel's terrace.

  • Johannes Burtscher, Patrick Schaefer, Luiz Felipe Garcia e Souza, Pablo Garcia-Roves, Enrico Calzia, Stephen Dozier, Ignazio Prieto, Verena Laner, Erich Gnaiger, Devin Manning, Pedro Neves after the Lunch Action.

  • Johannes Burtscher giving an interesting lecture on his results about oxidative phosphorylation in the healthy and epileptic mouse brain.

  • Pablo Garcia-Roves talking about the technical perspective of high-resolution respirometry in permeabilized skinned muscle fibers from different mouse models.

  • David Marcinek showing his results on mitochondrial redox stress.

  • Wonderful view down to Kuehtai with a fantastic natural surrounding.

  • Beautiful alpine roses, a typical vegetation in this alpine area.

  • Due to a perfect sunny Sunday the excursion to the Dreiseen Hütte can take place.

  • Chantal Pileggi, Dita Pelnena, Ed Ojuka and Pablo Garcia-Roves enjoying the beautiful and impressing surrounding.

  • Gloria-Maria Keppner and Adela Krajcova in front of the lake called Hirschebensee at a height of 2,164 m.

  • Taking pictures in this amazing natural environment is a 'must'.

  • Some brave participants used this lake for cooling down and enjoying the bath in this alpine lake (16°C).

  • A perfect snapshot of Erich head jumping into the water.

  • Zuzana Sumbalova, Elisa Calabria and Kristen Scholten enjoying this sunny day.

  • Verena Laner, Valentina Dikova, Carolina Doerrier and Chiara Volani.

  • Anthony Molina in front of an impressive alpine territory.

  • Finally arrived at the Dreiseen Hütte.

  • Everybody relaxing at the Dreiseen Hütte.

  • Michaela Markova taking a rest, well-deserved.

  • A pleasant sitting-together of mitochondriacs.

  • Another relaxed round-table: David Marcinek, P. Darrell Neufer with his wife Robyn and Justin Perry.

  • Beautiful view

  • Erich and his piece of art.

  • Watching the final game of the European soccer championship.

  • Pablo Garcia-Roves giving his lecture on design and implementation of systems biology approaches to integrate heterogenic data in biomedical research.

  • Michael Drinnan letting Datlab out of its box and talking about his visions with the software.

  • Martin Burtscher trying to answer the question whether living and exercising at moderate altitude is health risk or even benefit.

  • Meagan McManus talking about a multi-tiered mitochondrial approach to predictive biomarkers of neurodegenerative disease.

  • If muscle mitochondrial adaptations occur in response to high altitude is answered by Adam Chicco.

  • Gerald Maarman explaining that melatonin rescues uric acid-induced impairment of respiration in myotubes.

  • Training session with Stefan Moser (Sporttherapie Mag. Huber, AT).

  • Pedro and Ignazio being highly concentrated with this exercise.

  • Dita, Zuzana and Johannes carefully following the instructions by Stefan.

  • Up and down by Ignazio and Pedro.

  • Johannes and Erich performing a demanding exercise.

  • Patrick and Enrico enjoy playing with the ball.

  • Trainer Stefan carefully watching the performance of Pedro and Ignazio.

  • Dita and Zuzana trying to do the exercise precisely.

  • Energetic ball playing of Johannes Burtscher and Erich Gnaiger.

  • How should this exercise be done?

  • Verena Laner and Pedro Neves proudly showing the MitoFit-logo.

  • Part of the MitoFit-family: Verena Laner, Carolina Doerrier and Georgia Lamberti from Oroboros Instruments.

  • Brian Irving, Verena Laner, Adela Krajcova, Michaela Markova, Anthony Molina and Gloria-Maria Keppner enjoying the MitoFit Science Camp.

  • Back to work.

  • Giorgia Lamberti explaining her measurements and results.

  • Chiara Volani and Carolina Doerrier working on the DL7 proficiency test.

  • Gerald Maarman and Prisca Osiki.

  • Another scientific meeting in private between Patrick Schaefer, Devin Manning and Luiz Felipe Garcia e Souza.

  • Exchange between O2k-users.

  • Gloria-Maria Keppner and Michaela Markova working late night.

  • Enrico Calzia, Dominik Pesta and Tomas Jelenik.

  • Showing measurements on HEK-cells - Erich Gnaiger and Giorgia Lamberti (Oroboros Instruments).

  • The MitoFit Science Camp-Team.


Oroboros Scholarships

An Oroboros scholarship has been awarded to the following persons for the presentation of his/her research:
Michaela Markova (Charles University in Prague, CZ)
Adela Krajcova (Charles University in Prague, CZ)
Patrick Schäfer (University of Ulm, DE)
Dita Pelnena (Latvian Biomedical Research and Study Centre, Riga, LV)
Norma Lilia Morales Garcia (National University of Mexico, Mexico City, MX)
Christina Uribe Alvarez (National University of Mexico, Mexico City, MX)
Chantal Pileggi (University of Auckland, NZ)
Nicole Bezuidenhout (University of Cape Town, ZA)
Prisca Osiki (University of Cape Town, ZA)


Poster Awards

Poster Awards were presented to the following persons in recognition of their outstanding posters:


Participants, abstracts, programme

» MitoFit SC 2016 Participants and abstracts.


» Programme Bioblast pdf
A1: Diagnostic substrate-uncoupler-inhibitor titration (SUIT) protocols of mitochondrial fitness.
A2: Quality management system for mitochondrial respirometry: the MitoFit proficiency test with a real-time Demo-Experiment (SUIT reference protocol).
A3: DatLab 7 proficiency test
B1: Mitochondrial assays in blood cells: Blood cell preparation, cryopreservation, respiration.
B2: Cell culture models versus tissue samples: - Brain and neuronal cells - Adipose tissue - Other.
B3: Mitochondrial studies in neurodegeneration.
C1: Skeletal and cardiac muscle fibres - towards a data repository.
Working groups will be defined for the following topics: hyperoxic vs. normoxic, media, buffers, preparational assays, dry weight vs. wet weight, normalization (tissue vs. mt-markers; flux control ratios; flux control factors; statistical critique of RCR), quality control and nomenclature.
C2: Excursion: Dreiseenhütte.
D1: Towards a database on mt-respiratory physiology: from laboratory standards to inter-laboratory harmonization.
D2: Mitochondrial health: molecules, cells and tissues.
D3: MoTrPAC and MitoEAGLE
E1: MitoEAGLE and parallel IOC112 O2k-Workshop.
E2: MitoEAGLE and parallel IOC112 O2k-Workshop.


July 07 Th 08 Fr 09 Sa 10 Su 11 Mo 12 Tu 13 We
07:00-08:00 Arrival Yoga with Meagan Yoga with Meagan MitoFit training I with Stefan HIT-Training with Verena
08:00-09:00 Break(fast) Break(fast) Break(fast) Break(fast) Break(fast) Breakfast
09:00-10:15 Topic A1 Topic B1 Topic C1 Topic D1 Topic E1, VO2max tests Departure
10:45-12:00
12:00-15:00 Lunch & Action / e-bikes Soccer / Volleyball / e-bikes Excursion to Dreiseenhütte
in power / moderate / slow mode or
Innsbruck (bad weather alternative)		 Volleyball / Athletics / MitoFit Training II with Stefan, e-bikes, VO2max tests 2 km walking-tests, VO2max tests, e-bikes
15:30-16:45 Topic A2 Topic B2 Topic D2 Topic E2
17:15-18:30
19:00-20:30 Dinner Dinner Dinner Dinner Dinner Dinner
20:30-21:15 Welcome by the organizers A3: DatLab 7 proficiency test B3: Evening topic European-Championship public viewing D3: MitoEAGLE and MoTrPAC MitoFit party


  • Topics are scheduled as a sequence of workshops to merge introductory lectures, panels, and poster flashes.
  • High-resolution respirometry (HRR) will be demonstrated at various levels, integrating an IOC (O2k-Workshop on HRR) into the MitoFit Science Camp, with an emphasis on instrumental quality control - towards MitoFit Proficiency Testing. DatLab 7 will be released and introduced as the software for quality control in HRR.
  • The programme is designed as a global introduction to the perspectives of the COST Action MitoEAGLE.
MiPArt Gallery: World health worries by Odra Noel (2012)
MitoEAGLE theme
MitoEAGLE aims at providing the first standardized measures to link mitochondrial and physiome performance (heart rate, BMI, blood pressure, blood chemistry, VO2max) to understand the myriad of factors that play a role in mitochondrial physiology. The ambitious objective to optimally harmonize protocols across research groups cannot be achieved through projects with limited numbers of partners. Therefore, the COST framework represents an ideal instrument which allows bringing together many groups and experts in the diverse field of mitochondrial physiology. The aim of this Action is to form a unique well-coordinated network of senior researchers and young investigators, to include well established stakeholders, and to establish a spirit of mentorship and collaboration in contrast to fierce competition which characterized early decades of bioenergetics. The COST Action MitoEAGLE aims at developing a quality management system (QMS), defining common standards for measurements in the field of mitochondrial function.- MitoEAGLE may be seen as too ambitious in the short term, but indispensable at large.
Increase the value and reduce the noise
Enthusiastic and authentic opinion leaders can bring about a change to increase the value and reduce the noise in mitochondrial research specifically, biomedicine at large, and in our society in general. Millions of Euros are wasted on research that is lacking coherent standards. Prespecified and time-stamped protocols are needed, and researchers need to be introduced into adhering to publicly deposited protocols in practice (Begley, Ioannidis 2015). MitoEAGLE recommendations will provide practical guidelines for students, scientists and stakeholders.
Further details: MitoEAGLE


» For the programme in detail please see: MitoFit_SC_2016_Programme information


MitoEAGLE: towards the 1st MC Meeting

The sessions on the COST Action MitoEAGLE at the MitoFit Science Camp provided an excellent opportunity to discuss some key topics in preparation of the 1st Management Committee (MC) Meeting, including several Management Committee Members who participated in the full MitoFit SC or joined specifically for this discussion.
The Working Groups (WG) were divided into Task Groups. This was particularly important for the large WG1 (Standard operating procedures and user requirement document: Protocols, terminology, documentation). The tentative WG5 (Dissemination and training) could thereby be incorporated into WG1.


Chair, Vicechair and Steering Committee
All suggestions may be turned over at the 1st Management Committee Meeting.
Among the participants, assuming that the Main Proposer (Erich Gnaiger) will be nominated as Chair of the COST Action MitoEAGLE, Elisa Calabria was suggested as the Vicechair to be nominated at the 1st MC Meeting.
Steering Committee
A Steering Committee should be nominated, consisting of the Chair, Vicechair, WG leaders, regional representatives (not MC members) beyond the limits of COST countries, a Financial Officer (Jaap Keijer should be consulted for suggestions), and a General Secretary (Christina Plattner).
The following criteria should be kept in mind for nominations: (1) Expertise; (2) Regions - add non-MC members as links to other continents; (3) Gender balance; (4) participation of Early Career Investigators (researchers in the time span of up to 8 years after the date of obtaining the PhD/doctorate). Among the participants, the following WG leaders were suggested, and a co-leader should be additionally nominated for the 1st MC Meeting:
WG leaders
  • 2016-09-06: Erich is attending the COST Preparatory Workshop for Main Proposers at the COST Office in Brussels. Here are some relevent suggestions from COST:
  • WG leaders have to be MC members, exceptionally MC substitute.
  • Colleagues from Inclusiveness Target Countries (ITC) and Early Career Investigator (ECI) should be well represented as WG leaders, and rotation of some MC members for ECI should be considered by some COST National Coordinators.
  • A rotational system can be implemented and is even encouraged. WG co-leaders may be elected at the MC meeting with the intention to rotate at a given time point between WG leader and WG co-leader. Both should be MC members.
  • Define in advance Short Term Scientific Missions with ITC and ECI in mind, decide on assessment criteria.


International partner representatives


Working groups with a list of task forces
WG1: MitoEAGLE protocols, terminology, documentation
WG2: MitoEAGLE data: muscle
WG3: MitoEAGLE data: tissues
WG4: MitoEAGLE data: blood and cultured cells


Partners

MitoFit MUI Logo rz rgb.jpg LFU-Innsbruck-logo.png Oroboros WGT HLZ Kuehtai Sporttherapie Huber Tirol Logo Standortagentur.jpg


Additional support

Stealth Biotherapeutics
Oroboros
MitoGlobal
Listed under MitoGlobal Events.
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