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{{MitoPedia chapter}} ::::» [[MitoPedia: Respiratory control ratios]]

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{{MitoPedia without banner ...'': for [[tissue preparation]], stainless steel, antimagnetic. One pair is recommended for insertion of the sample into the [[O2k-chamber]] and for handling in co

{{MitoPedia {{MitoPedia concepts

'''»''' [[MitoPedia: Respiratory states]] == [[IOC Recommended Reading | Recommended reading]] ==

{{MitoPedia without banner ...'': for [[tissue preparation]], stainless steel, antimagnetic. One pair is recommended for placing the tissue sample onto the [[Microbalance 120 g | microbalance]

== IOC recommended reading == ::::» '''[[IOC recommended reading]]'''

...til the meeting [[MitoEAGLE Barcelona 2017]], by (i) elaborating the terms recommended in Table 1, and (ii) addition of missing terms for discussion and recommend ...ld be defined, possible extending beyond 'respiratory states' to include [[MitoPedia: Respiratory control ratios |respiratory control ratios]].

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== Recommended reading == Please click »[[IOC Recommended Reading| here]]«

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{{MitoPedia without banner ...fixed needle with rounded tip: 51 mm length, 0.41 mm inner diameter. It is recommended for injections of suspensions of isolated mitochondria.

{{MitoPedia ...R assay on the respiration using living cells, the following protocols are recommended: [[SUIT 003 AmR ce D058]] with AmR and [[SUIT 003 AmR ce D059]] with carrie

{{MitoPedia without banner ...-click on the text. It does not influence continuous data recording. It is recommended to maintain automatic panning on during the experiment, except for specific

{{MitoPedia ...metry. For highest-level accuracy of calculation of oxygen pressure, it is recommended to compare at regular intervals the barometric pressure recording provided

{{MitoPedia ...s from barometric pressure within small gas bubbles and is, therefore, not recommended. To equilibrate an aqueous solution ata known partial pressure of oxygen [k

{{MitoPedia ...ΔpH and [[Mitochondrial membrane potential|mt-membrane potential]]. It is recommended to use the lowest possible concentration of nigericin, which creates a maxi

{{MitoPedia ...dermal, inhalation). Dust mask or eyeshields, faceshields and gloves are recommended by the preparation.</span>

{{MitoPedia |info=[[MitoPedia: BEC]]

::: '''MitoPedia: SUIT''' - '''Substrate-uncoupler-inhibitor titration protocols''' ...Fit.png|right|190px|link=http://www.bioblast.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]

{{MitoPedia ::: '''[[MitoPedia: SUIT]]'''

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{{MitoPedia ...h peroxidase type II in fluorometric measurement of peroxide production is recommended by [[Tretter L|Laszlo Tretter]].

{{MitoPedia without banner ...the bottom). The default name is the sequential number of the event. It is recommended to edit event labels with a minimum number of characters, and to explain th

{{MitoPedia ...ed and [[cytochrome c|cytochrome ''c'']] can be released. Therefore, it is recommended to add [[cytochrome c|cytochrome ''c'']] after the optimal digitonin concen

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{{MitoPedia ::: '''[[MitoPedia: SUIT]]''' - Protocol to analyse AmR effect on living cells respiration

{{MitoPedia ::: '''[[MitoPedia: SUIT]]''' - Carrier control protocol to [[SUIT-003 AmR ce D058]]

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{{MitoPedia == MitoPedia: Respiratory states ==

{{MitoPedia without banner ::::* Recommended: Intel i5 or equivalent CPU, 4GB RAM, Windows 10, SSD

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== IOC recommended reading == ::::» '''[[IOC recommended reading]]'''

{{MitoPedia {{MitoPedia concepts

::::# [[MitoPedia:_SUIT |SUIT protocols]] - The [[SUITbrowser]] helps you find the best SUIT == IOC recommended reading ==

== IOC recommended reading == ::::» '''[[IOC recommended reading]]'''

{{MitoPedia {{MitoPedia concepts

== IOC recommended reading == ::::» '''[[IOC recommended reading]]'''

{{MitoPedia :::* When MiR05 is used, the addition of [[pyruvate]] is recommended to provide an external fuel substrate.

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{{MitoPedia :::* A [[Carrier control titrations|carrier control]] with DMSO is recommended, [[SUIT-003 O2 ce D062]] is provided with this purpose.

== Recommended reading == : » [[IOC recommended reading]]

{{MitoPedia {{MitoPedia O2k and high-resolution respirometry|type=Inhibitor ETS

{{MitoPedia without banner |info=[[MitoPedia: DatLab]]

{{MitoPedia without banner ::::* Replacement of a damaged POS-Holder is recommended. For ordering spare parts: [https://www.oroboros.at/index.php/o2k-products/

{{MitoPedia ::::* [[SUIT-003 O2 ce D038]] is recommended to be used in parallel to obtain more information on Crabtree effect.

{{MitoPedia ...lyse coupling control in [[mitochondrial preparations |mtprep]] (''see'' [[MitoPedia: Sample preparations]]) at [[Succinate pathway control state]].

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{{MitoPedia Even if 1 µM is recommended as the working concentration in most of the publications it is highly relev

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{{MitoPedia ::: '''[[MitoPedia: SUIT]]''' - [[coupling-control protocol]] (CCP) with pyruvate and cell mem

{{MitoPedia {{MitoPedia concepts

{{MitoPedia :::* When MiR05 is used, the addition of [[pyruvate]] is recommended to provide an external fuel substrate.

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{{MitoPedia ...ation on each experimental day. After performing a zero calibration, it is recommended not running other experiments on the same day. Even after standard cleaning

::::::» [[MitoPedia: DatLab]] == IOC recommended reading ==

{{MitoPedia {{MitoPedia concepts}}

{{MitoPedia The protocols recommended in the SUIT-003 have been designed to study the coupling control state of l

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{{MitoPedia without banner :::: '''R1 is higher than recommended:'''

...|60px|link=https://wiki.oroboros.at/index.php/MitoPedia:_O2k-Open_Support |MitoPedia: O2k-Open Support]] == IOC recommended reading ==

{{MitoPedia ::: For the specific case of microalgal cells it is recommended to prepare higher concentration:

{{MitoPedia :::: Recommended reading

{{MitoPedia ...R assay on the respiration using living cells, the following protocols are recommended: [[SUIT 003 AmR ce D058]] with AmR and [[SUIT 003 AmR ce D059]] with carrie

::::# [[MitoPedia:_SUIT |SUIT protocols]] - The [[SUITbrowser]] helps you find the best SUIT == IOC recommended reading ==

{{MitoPedia :::* It is recommended to run control with carrier titrations in parallel, since DMSO may affect l

== IOC recommended reading == ::::» '''[[IOC recommended reading]]'''

== IOC recommended reading == ::::» '''[[IOC recommended reading]]'''

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{{MitoPedia ::: '''[[MitoPedia: SUIT]]'''

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{{MitoPedia ...in ROUTINE respiration may require up to 10-15 min, waiting longer is not recommended.

{{MitoPedia ::: '''[[MitoPedia: SUIT]]'''

{{MitoPedia ...MiR05-Kit showed a linear correlation with the oxygen concentration, it is recommended to change the oxygen level during the experiment.

{{MitoPedia {{MitoPedia concepts

== Recommended reading == [[Image:MitoPedia-text.jpg|left|100px|link=MitoPedia|MitoPedia]]

{{MitoPedia :::- We do not generally recommended the addition of permeabilized cells (already permeabilized), however we rec

{{MitoPedia ...s the ratio of body mass to height squared (BMI=''M''·''H''^-2), recommended by the WHO as a general indicator of underweight (BMI<18.5 kg·m<sup>-2</su

{{MitoPedia {{MitoPedia concepts

{{MitoPedia :::* It is recommended to run a chemical background without any sample to test the effect of the c

{{MitoPedia :::- We do not generally recommended the addition of already permeabilized cells, but we recommend to perform th

{{MitoPedia without banner ...ng settings applied in DatLab when recording the data with the O2k. Usng [[MitoPedia: SUIT |SUIT protocols]], a sequence of respiratory steady-states is measure

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{{MitoPedia ...states and in ROX state measured in [[MiR05-Kit]] medium. It is therefore recommended to vary the oxygen level in the O2k-Chamber several times during an experim

{{MitoPedia ...ent to avoid carry-over of inhibitors. The addition of liver homogenate is recommended in the washing protocol to bind strong inhibitors.

{{MitoPedia {{MitoPedia concepts

== IOC recommended reading == ::::» '''[[IOC recommended reading]]'''

{{MitoPedia ...nt to avoid carry-over of inhibitors. The addition of sample homogenate is recommended in the washing protocol to bind strong inhibitors.

:::: It is recommended that participants prepare for their first live sessions by getting familiar :::: The materials for self-study, with the names and links to materials, are recommended to be studied before the respective training sessions of the Virtual O2k-Wo

{{MitoPedia :::* It is recommended to run a chemical background without any sample to test the effect of the c

{{MitoPedia ...arry-over of uncoupler and inhibitors. The addition of liver homogenate is recommended in the washing protocol to bind strong inhibitors.

:::: It is recommended that participants prepare for their first live sessions by getting familiar :::: The materials for self-study, with the names and links to materials, are recommended to be studied before the respective training sessions of the Virtual O2k-Wo

{{MitoPedia ...ent to avoid carry-over of inhibitors. The addition of liver homogenate is recommended in the washing protocol to bind strong inhibitors.

{{MitoPedia without banner ...nges for the axes change the appearance of data dramatically. It is highly recommended to use reference layouts.

{{MitoPedia {{MitoPedia concepts

{{MitoPedia ...ed in SUIT protocols with [[living cells]]. The [[SUIT-003]] protocols are recommended for this purpose. Use the [https://suitbrowser.oroboros.at/ SUITbrowser] to

{{MitoPedia {{MitoPedia concepts

== IOC recommended reading == ::::» '''[[IOC recommended reading]]'''

{{MitoPedia {{MitoPedia concepts

{{MitoPedia ...rents should be selected for each LED. Therefore, it might be difficult to recommended a LED current for this application that is is good for all LEDs. Note: This

{{MitoPedia {{MitoPedia concepts

{{MitoPedia ...R assay on the respiration using living cells, the following protocols are recommended: [[SUIT 003 AmR ce D058]] with AmR and [[SUIT 003 AmR ce D059]] with carrie

== IOC recommended reading == ::::» '''[[IOC recommended reading]]'''

== IOC recommended reading == ::::» '''[[IOC recommended reading]]'''

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== IOC recommended reading == ::::» '''[[IOC recommended reading]]'''

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{{MitoPedia ...hen the plasma membrane is removed. Therefore, the addition of catalase is recommended when working with mitochondrial preparations, to consume any H₂O

{{MitoPedia :::* It is recommended to run a chemical background assay without any sample to test the effect of

{{MitoPedia ...A cell-count concentration ''C''_ce of 1.0 Mx·mL^-1 is recommended for many cultured cells, including different cancer or immortalized cell li

== IOC recommended reading == ::::» '''[[IOC recommended reading]]'''

== IOC recommended reading == ::::» '''[[IOC recommended reading]]'''

{{MitoPedia ...R assay on the respiration using living cells, the following protocols are recommended: [[SUIT-003 AmR ce D058]] with AmR and [[SUIT-003 AmR ce D059]] with carrie

{{MitoPedia {{MitoPedia concepts

{{MitoPedia ...phosphorylation system. [[cytochrome c|Cytochrome ''c'']] addition is also recommended to avoid any limitation by cytochrome ''c'' release.

'''»''' [[MitoPedia: Respiratory states]] == Recommended reading ==

{{MitoPedia ...al O2 background |instrumental O₂ background tests]]. It is not recommended to use dithionite in experiments with biological samples or several multise

'''»''' [[MitoPedia: Respiratory states]] == [[IOC Recommended Reading| Recommended reading]] ==

== IOC recommended reading == ::::» '''[[IOC recommended reading]]'''

:::: 15:00 Innovations in DatLab 7 - and more in [[MitoPedia: DatLab]] === IOC recommended reading ===

{{MitoPedia {{MitoPedia concepts}}

== Recommended reading == :» [[IOC Recommended Reading| More details]]

{{MitoPedia {{MitoPedia concepts

{{MitoPedia {{MitoPedia O2k and high-resolution respirometry

...but not for women. Furthermore, gender and age specific cut-off values are recommended in this population. {{MitoPedia: BME and mitObesity}}

{{MitoPedia ...(e.g., ADP, NADH) are forbidden to be refrozen and new frozen aliquots are recommended to be used in each experiment.

== IOC recommended reading == ::::» '''[[IOC recommended reading]]'''

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...m the initial illness or develop after the recovery. Spa rehabilitation is recommended for patients with post COVID-19 syndrome. In our previous study deficit of ...n spa rehabilitation with coenzyme Q₁₀ supplementation could be recommended to the patients with post COVID-19 syndrome.

{{MitoPedia ...the OROBOROS Mitochondrial Research Laboratory'''. Up to now, we used and recommended FCCP as an uncoupler in high-resolution respirometry. Recently we compared

{{MitoPedia ...c-215789''', store at +4 °C; '''M = 324.13 g·mol^-1''', which is recommended to use with the Q-Module.

== Recommended reading == [[Image:MitoPedia-text.jpg|left|100px|link=MitoPedia|MitoPedia]]

{{MitoPedia ...fatty acids or acylcarnitines might inhibit respiration, therefore, it is recommended to test the optimum concentration for new samples or different acylcarnitin

'''»''' [[MitoPedia: Respiratory states]] == Recommended reading ==

{{MiPNet pdf page linking to MitoPedia}} ::2) Disrupt big lumps mechanically. It is recommended to do this before adding water, because during dissolution these lumps do n

{{MitoPedia ..., the excitation maximum is 511 nm and the emission maximum is 534 nm. The recommended excitation and emission wavelengths in PBS are 488 and 515-575 nm, respecti

{{MitoPedia {{MitoPedia concepts}}

...pen Access''' online journal '''[[Mitochondr Physiol Network]]''', in '''[[MitoPedia]]''', and the new '''online preprint journal [[MitoFit Preprints]]'''. Comments: optional, but recommended

{{MitoPedia ...ent to avoid carry-over of inhibitors. The addition of liver homogenate is recommended in the washing protocol to bind strong inhibitors.

{{MitoPedia without banner ...or any other layout can be modified and saved under Lab layouts, which is recommended for a team using project-specific layouts.

{{MitoPedia ::::* Q-''n'' is recommended by IUPAC for ubiquinone-''n'': https://iupac.qmul.ac.uk/misc/quinone.html

{{MitoPedia ...arry-over of uncoupler and inhibitors. The addition of liver homogenate is recommended in the washing protocol to bind strong inhibitors.

{{MitoPedia ...ent to avoid carry-over of inhibitors. The addition of liver homogenate is recommended in the washing protocol to bind strong inhibitors.

{{MitoPedia ...lyse coupling control in [[mitochondrial preparations |mtprep]] (''see'' [[MitoPedia: Sample preparations]]) at a defined [[electron-transfer-pathway state]].

{{MitoPedia without banner ...you recommend? I just tried with a solution (outside of the Oroboros O2k) recommended from the factory (0.05 M H₂SO₄, 0.02 M KI with additi

{{MitoPedia ...arry-over of uncoupler and inhibitors. The addition of liver homogenate is recommended in the washing protocol to bind strong inhibitors.

== IOC recommended reading == ::::» '''[[IOC recommended reading]]'''

{{MitoPedia ...hose for Magnesium Green. However, the [[Magnesium_Green#Instrument|values recommended for Magnesium Green]] will provide a good starting point.

{{MitoPedia {{MitoPedia methods

== Recommended reading == [[Image:MitoPedia-text.jpg|left|100px|link=MitoPedia|MitoPedia]]

== IOC recommended reading == ::::» '''[[IOC recommended reading]]'''

== IOC recommended reading == ::::» '''[[IOC recommended reading]]'''

{{MitoPedia |info=[[BEC 2020.1]], [[Gnaiger 2020 BEC MitoPathways]], [[MitoPedia: Respiratory states]]

== IOC recommended reading == ::::» '''[[IOC recommended reading]]'''

== IOC recommended reading == ::::» '''[[IOC recommended reading]]'''

{{MitoPedia without banner ::::* Carry-over of inhibitors or uncouplers: It is recommended to wash the O2k-chambers with cells, [[tissue homogenate | thom]] or [[Isol

== IOC recommended reading == ::::» '''[[IOC recommended reading]]'''

== IOC recommended reading == ::::» '''[[IOC recommended reading]]'''

== IOC recommended reading == ::::» '''[[IOC recommended reading]]'''

== IOC recommended reading == ::::» '''[[IOC recommended reading]]'''

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== IOC recommended reading == ::::» '''[[IOC recommended reading]]'''

...edia|MitoPedia]] <span style="font-size:105%; color:##424242"> '''Keywords—MitoPedia''' - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>></span> '''Keywords—[[MitoPedia]]'''.

{{MitoPedia {{MitoPedia concepts

== IOC recommended reading == ::::» '''[[IOC recommended reading]]'''

== IOC recommended reading == :» [[IOC recommended reading |More details]]

{{MitoPedia == Related MitoPedia pages ==

...re closely aligned with the WHO Child Growth Standards at 5 years, and the recommended adult cut-offs for overweight and obesity at 19 years. They fill the gap in {{Template:MitoPedia: BME and mitObesity}}

== Recommended reading == :::: Please click »[[IOC Recommended Reading| here]]«

{{MitoPedia without banner :::: It is highly recommended that participants prepare for their sessions by going through the self-stud

{{MitoPedia {{MitoPedia concepts

{{MitoPedia without banner ...|application specific settings]] are only suggestions to start with. It is recommended to optimize the light intensity specifically for each application.

== IOC recommended reading == ::::» '''[[IOC recommended reading]]'''

File:B3.jpg|Mike Davis and Erich Gnaiger discuss the [[MitoPedia:_SUIT#SUIT_protocols |SUIT protocols]]. == Recommended reading ==

== IOC recommended reading == ::::» '''[[IOC recommended reading]]'''

{{MitoPedia ...l-defined peaks for oxidation and reduction potentials are observed, it is recommended to run more cycles to check whether additional peaks are detected or the sh

=== Recommended IOC reading === Please click »[[IOC Recommended Reading| here]]«

{{MitoPedia without banner ::::# Check the location of your saved files. It is not recommended to record your files directly to a server since the DLD file is rewritten e

{{MitoPedia :::: The use of SI and IUPAC symbols and units is recommended for clarity and consistency of presenting concepts and reporting data, as f

== IOC recommended reading == ::::» '''[[IOC recommended reading]]'''

| [[MitoPedia: DatLab]] | [[MitoPedia: SUIT]]

...ub>2</sub>O₂ stock solution needs to be freshly prepared, it is recommended to perform a background H₂O₂ calibration before each ...UIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]

{{MitoPedia without banner ...[[coupling-control state]]s and [[Electron-transfer-pathway state]]s. A [[MitoPedia:_SUIT|library]] of evaluated and tested standard DL-Protocols is provided b

{{MitoPedia without banner ...2</sub> calibration and instrumental O₂ background tests. 20 is recommended for data recording and analysis in typical SUIT experiments. When a larger

...ical overview on high-resolution respirometry (Pesta and Gnaiger 2012), is recommended for reading as a good preparation for the workshop. In addition to the lite ...your memory on a few conventional bioenergetic terms, take a look in the [[MitoPedia]] on respiratory states - we will take some time to discuss the rationale f

* Further reading: [[IOC Recommended Reading]] * [[MitoPedia:_Respiratory_states]]

{{MitoPedia without banner ...before the decline of O2 slope neg.). In case of any doubt, it is strongly recommended to repeat the instrumental O₂ background test, taking care of th

...ter the initial AmR calibration, ensure that the sensitivity is within the recommended range.'''<br> | width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]

{{MitoPedia {{MitoPedia methods

According to clinical pharmacokinetic studies, the recommended therapeutic dose of metformin is within the range of 5-20 µM [4]. However, ::::::[[MitoPedia:_SUIT]]

* Further reading: [[IOC recommended reading]] * [[MitoPedia:_Respiratory_states]]

{{MitoPedia ...ted mitochondria. This approach is well tested for yeast cells, but is not recommended for mammalian cells which may loose viability due to the high shear stress

{{MitoPedia :» MitoPedia topic: [[Mitochondrial preparations]]

{{MitoPedia topics}} {{MitoPedia without banner

{{MitoPedia ::::* The recommended approach is:

| [[MitoPedia: SUIT]] :::: The following hotels are recommended - please mention 'MitoEAGLE' in your reservation

:::: 2020-03-12: ''Generally recommended precautions suggest to restrict our seminars to internal small-scale meetin ::::::» [https://www.bioblast.at/index.php/Protonmotive_force MitoPedia: Protonmotive force]

| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]] | width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]

| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]] | width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]