Calcium Green

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MitoPedia

Calcium Green

Description

Calcium Green denotes a family of extrinsic fluorophores applied for measurement of Ca2+ concentration.

Abbreviation: CaG


MitoPedia methods: Fluorometry 

Sources and applications

Calcium GreenTM is a registered trademark and available from Life Technologies (former Invitrogen) in several formulations.
All fluorophores of the Calcium Green family have the same fluorophoric moiety in their molecular structure and therefore near identical optical properties. They differ in their KD values and are suitable for measuring different ranges of calcium concentrations.
  • Calcium Green 1: KD = 190 nM
  • Calcium Green-2: KD = 550 nM
  • Calcium Green-5N: KD = 14000 nM


Measurement
See Calcium for method-independent considerations related to measuring calcium levels, covering e.g.
  • Considerations before starting a project involving calcium measurements;
  • Ca2+ buffering;
  • Calculation of free Ca2+ concentrations;
  • Ca2+ calibration buffers.
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O2k-Open Support


MitoPedia O2k and high-resolution respirometry: O2k-Open Support 

Ca measurement

See O2k-Fluo LED2-Module for specific aspects of fluorescence measurements using the O2k.
On the fluorophores producers homepage detailed product information is available
General information on measuring calcium, e.g.
The three Calcium Green fluorophores and Magnesium Green share the same fluorophore moiety in their molecular structure, therefore the optical properties are very similar and the same fluorescence sensor and filter set can be used. Since the used concentration of Calcium Green might vary according to application, the optimum light intensity and amplification used may be different to those for Magnesium Green. However, the values recommended for Magnesium Green will provide a good starting point.


Signal and output

  1. Signal: The O2k-Fluo LED2-Module is operated through the amperometric (Amp)-Channel of the O2k, with electric current (ampere [A]) as the primary signal.
  2. Output: The focus of the output with Calcium Green is on Type A: Concentration, and Type C: Force, potential.


References