Startup O2k-Respirometer: Difference between revisions
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{{Product | {{Product | ||
|description='''Startup O2k-Respirometer''' - the experimental system complete for basic [[High-Resolution Respirometry|high-resolution respirometry]] (HRR). The O2k-Respirometer includes the [[O2k-Main Unit]] with stainless steel housing, [[O2k-Assembly Kit]], two [[OroboPOS]] (polarographic oxygen sensors) and [[OroboPOS-Service Kit]], [[DatLab]] software, the [[ISS-Integrated Suction System]] and the [[O2k-Titration Set]]. The O2k-Respirometer supports all add-on O2k-Modules of the [[Oroboros O2k]], and is a component of the [[O2k-FluoRespirometer]]. | |description='''Startup O2k-Respirometer''' - the experimental system complete for basic [[High-Resolution Respirometry|high-resolution respirometry]] (HRR). The O2k-Respirometer includes the [[O2k-Main Unit]] with stainless steel housing, [[O2k-Assembly Kit]], two [[OroboPOS]] (polarographic oxygen sensors) and [[OroboPOS-Service Kit]], [[DatLab]] software, the [[ISS-Integrated Suction System]] and the [[O2k-Titration Set]]. The O2k-Respirometer supports all add-on O2k-Modules of the [[Oroboros O2k]], and is a component of the [[O2k-FluoRespirometer]]. | ||
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|product image=[[Image:Startup O2k-Respirometer notitle.png|200px|right]] | |product image=[[Image:Startup O2k-Respirometer notitle.png|200px|right]] | ||
}} | }} | ||
::::* The '''O2k-Core''' does not include, but fully supports and is required for the add-on Modules of the [[Oroboros O2k]], such as the [[Titration- | [[File:Startup O2k-Respirometer.png|right|600px|O2k-FluoRespirometer]] | ||
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::::*The '''O2k-Core''' does not include, but fully supports and is required for the add-on Modules of the [[Oroboros_O2k|Oroboros O2k]], such as the [[Titration-Injection_microPump|TIP2k]], [[O2k-Fluo_LED2-Module|O2k-Fluo LED2-Module]], [[O2k-TPP+_ISE-Module|O2k-TPP+ ISE-Module]], [[O2k-pH_ISE-Module|O2k-pH ISE-Module]], [[O2k-NO_Amp-Module|O2k-NO Amp-Module]]. | |||
::::* A PC is required (not included), connecting DatLab to the O2k via a USB port. For PC requirements, see [[DatLab# | : | ||
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::::*A PC is required (not included), connecting DatLab to the O2k via a USB port. For PC requirements, see [[DatLab#PC_requirements|here]]. | |||
[[File:Questions.jpg|left|40px]] | [[File:Questions.jpg|left|40px|Questions.jpg]] | ||
<div class="toccolours mw-collapsible mw-collapsed"> | <div class="toccolours mw-collapsible mw-collapsed"> | ||
::: <span style="font-size:105%; color:##424242">'''» Keywords'''</span> | : | ||
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:::<span style="font-size:105%; color:##424242">'''» Keywords'''</span> | |||
<div class="mw-collapsible-content"> | <div class="mw-collapsible-content"> | ||
::: '''Specific''' | : | ||
::::» [[DatLab]] | :: | ||
::::» [[DatLab# | :::'''Specific''' | ||
::::» [[ISS-Integrated Suction System]] | ::::» [[DatLab|DatLab]] | ||
::::» [[Oroboros O2k]] | ::::» [[DatLab#PC_requirements|DatLab PC requirements]] | ||
::::» [[O2k-Assembly Kit]] | ::::» [[ISS-Integrated_Suction_System|ISS-Integrated Suction System]] | ||
::::» [[O2k-Main Unit]] | ::::» [[Oroboros_O2k|Oroboros O2k]] | ||
::::» [[O2k-Titration Set]] | ::::» [[O2k-Assembly_Kit|O2k-Assembly Kit]] | ||
::::» [[OroboPOS]] | ::::» [[O2k-Main_Unit|O2k-Main Unit]] | ||
::::» [[OroboPOS-Service Kit]] | ::::» [[O2k-Titration_Set|O2k-Titration Set]] | ||
::::» [[MiPNet06. | ::::» [[OroboPOS|OroboPOS]] | ||
::::» [[OroboPOS-Service_Kit|OroboPOS-Service Kit]] | |||
::::» [[MiPNet06.05_Specifications|Oxygraph specifications]] | |||
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:::'''General''' | |||
::::» [[O2k-FluoRespirometer|O2k-FluoRespirometer]] | |||
::::» [[O2k-Fluo_LED2-Module|O2k-Fluo LED2-Module]] | |||
::::» [[O2k_high-resolution_respirometry|O2k high-resolution respirometry]] | |||
::::» [[O2k-NO_Amp-Module|O2k-NO Amp-Module]] | |||
::::» [[O2k-pH_ISE-Module|O2k-pH ISE-Module]] | |||
::::» [[O2k-TPP+_ISE-Module|O2k-TPP+ ISE-Module]] | |||
::::» [[Titration-Injection_microPump|TIP2k]] | |||
</div> </div> | |||
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== The '''Startup O2k-Respirometer''' ''consists of'' == | |||
{{#ask:[[Category:Products]] [[Product type::Startup O2k-Respirometer]] | |||
{{#ask: [[Category:Products]] [[Product type::Startup O2k-Respirometer]] | |||
| ?description | | ?description | ||
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== O2k-Core manual and selected protocols == | == O2k-Core manual and selected protocols == | ||
::::» <big>'''[[Media:MiPNet19. | |||
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::::» <big>'''[[Media:MiPNet19.18_O2k-Core_Manual.pdf|Contents: O2k-Core Manual DatLab 6.pdf]]'''</big> | |||
{{#ask:[[Category:Publications]] [[Additional label::O2k-Core]] | {{#ask:[[Category:Publications]] [[Additional label::O2k-Core]] |
Revision as of 08:52, 10 March 2018
Startup O2k-Respirometer
Description | Startup O2k-Respirometer - the experimental system complete for basic high-resolution respirometry (HRR). The O2k-Respirometer includes the O2k-Main Unit with stainless steel housing, O2k-Assembly Kit, two OroboPOS (polarographic oxygen sensors) and OroboPOS-Service Kit, DatLab software, the ISS-Integrated Suction System and the O2k-Titration Set. The O2k-Respirometer supports all add-on O2k-Modules of the Oroboros O2k, and is a component of the O2k-FluoRespirometer.
On-line display of oxygen flux (rate of respiration) is provided in addition to the conventional 'oxygraphic' plot of oxygen concentration over time. Highest signal stability minimizes the required amounts of biological sample, and provides the basis for resolution in the extreme low-oxygen range. Peltier temperature control provides a thermal stability at ±0.002 °C in the range of 4 °C to 47 °C at typical constant room temperature. Electronically controlled PVDF or PEEK stirrers are integrated in the two-chamber design of the O2k, and a barometric pressure transducer enables automatic oxygen calibrations implemented in the DatLab software. The O2k is a sole source apparatus with no other instruments meeting its specifications. |
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Product ID | 10022-02 |
Type | O2k |
Link | Gnaiger_2008_POS |
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- The O2k-Core does not include, but fully supports and is required for the add-on Modules of the Oroboros O2k, such as the TIP2k, O2k-Fluo LED2-Module, O2k-TPP+ ISE-Module, O2k-pH ISE-Module, O2k-NO Amp-Module.
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- A PC is required (not included), connecting DatLab to the O2k via a USB port. For PC requirements, see here.
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The Startup O2k-Respirometer consists of
O2k-Core manual and selected protocols
Chapter | View | Section | Last update |
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Gnaiger 2008 POS | Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial dysfunction in drug-induced toxicity (Dykens JA, Will Y, eds) John Wiley & Sons, Inc, Hoboken, NJ:327-52. | 2008 | |
Gnaiger 2020 BEC MitoPathways | published online 2020-12-30
| Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002 | 2020 |
MiPNet04.05 Titration-Injection | From step titration to ramp injection: Uncoupling by FCCP with TIP. | 1999-01 | |
MiPNet07.08 User information | » Versions | O2k-Manual: user information. PLEASE STUDY THIS MANUAL. | 2017-11-03 |
MiPNet08.09 CellRespiration | »Versions | High-resolution respirometry and coupling-control protocol with living cells: ROUTINE, LEAK, ET-pathway, ROX. | 2016-08-11 |
MiPNet08.12 IOC22 | NO effect on mitochondrial oxygen kinetics at low oxygen. O2k workshop Report. | 2011-12-11 | |
MiPNet12.01 Suppl T-issue | »Versions | MitoPathways at the Q-junction: mouse skeletal muscle fibers. | 2018-11-18 |
MiPNet12.11 MitoRespiration | »Versions | Flux control ratios in isolated mitochondria. OXPHOS capacity and respiratory control. | 2014-02-20 |
MiPNet17.03 Shredder vs Fibers | »Versions | Mitochondrial respiration in permeabilized fibers versus homogenate from fish liver and heart. An application study with the PBI-Shredder. | 2015-06-18 |
MiPNet19.18 O2k-Series G Core manual | »Versions | O2k-Core manual contents. | 2016-08-08 |
MiPNet19.18B POS-service | »Versions | Service of the polarographic oxygen sensor OroboPOS. | 2021-06-23 |
MiPNet19.18C DatLab 6: Guide | »Versions | DatLab-guide through the menus. | 2016-08-24 |
MiPNet19.18D O2k-Series G and DatLab 6: Calibration | »Versions | O2k-calibration by DatLab 6. | 2016-08-24 |
MiPNet19.18E O2 flux analysis - DatLab 6 | »Versions | Oxygen flux analysis: DatLab real-time. | 2016-08-08 |
MiPNet21.14 Reference sample HRR | »Versions | Development of a reference sample for HRR. | 2016-10-19 |
MiPNet21.16 DatLab 7 Innovations | DatLab 7: innovations. | 2019-09-23 | |
MiPNet22.16 DatLab 7 Innovations: DL-Protocols | » Versions | DatLab 7.4 Innovations: DL-Protocols | 2019-09-13 |
MiPNet23.12 DL-Protocols help | » Versions | DatLab Protocols: Help DatLab 7. | 2019-09-13 |
MiPNet24.06 Oxygen flux analysis - DatLab 7.4 | » Versions | Oxygen flux analysis with DatLab 7.4. | 2020-04-16 |
MiPNet24.08 Safranin Analysis Template | » Versions | Excel template for safranin data analysis. | 2020-05-13 |
MiPNet24.09 Data analysis of mt-membrane potential | » Versions | Data analysis of mt-membrane potential. | 2021-09-16 |
MiPNet25.14 TPP Analysis Template | » [https:.pdf Versions] | Excel template for TPP data analysis. | 2020-##-## |
MiPNet26.06 DatLab 7: Guide | »Versions | DatLab-guide through the menus. | 2021-06-02 |
MiPNet28.10 SmartPOS-service | » Versions | SmartPOS: Service | 2023-09-29 |
Pesta 2012 Methods Mol Biol | PMID: 22057559 | Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscle. Methods Mol Biol 810:25-58. https://doi.org/10.1007/978-1-61779-382-0_3 | 2012 |
MitoPedia O2k and high-resolution respirometry:
O2k hardware