O2k-TPP+ ISE-Module

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Oroboros Instruments

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O2k-TPP+ ISE-Module


Description O2k-TPP+ ISE-Module: 2 potentiometric ion-selective electrodes (ISE), ISE-Service Kit and 2 PEEK-Stoppers, supported by the Oroboros O2k (O2k-Series D and higher). It also includes the newest DatLab Version. The ISE module is designed with replaceable membranes making it possible to measure different ions such as TPP+ or Ca2+ with the same electrode housing1. The main application of the O2k-TPP+ ISE-Module is measurement of mitochondrial membrane potential with tetraphenylphosphonium (TPP+) or TPMP+.

1 There are other ion selective membranes available (e.g., from Sigma Aldrich), for a wide variety of ionic species. If cut to a circle with a diameter of 4 mm these membranes can be used in the ISE system. Many ions may also be detected by fluorimetric based methods.

Product ID 12300-03
Type O2k, O2k-Module, MultiSensor, Stopper
Link O2k-Modules, MiPNet15.03 O2k-MultiSensor-ISE
TPP new.jpg

O2k-TPP+ ISE-Module consists of:

TitleDescriptionProduct idProduct image
O-ring\Viton\12.5x1 mmO-ring\Viton\12.5x1 mm, for PVDF or PEEK O2k-Stoppers, box of 8 as spares. Two spare boxes of this product are standard components of the O2k-Assembly Kit (O2k-FluoRespirometer) as well as the O2k-TPP+ ISE-Module, O2k-pH ISE-Module and the O2k-NO Amp-Module.24310-02
O-ringViton12.5x1 mm.jpg
Stopper-SpacerStopper-Spacer (or gas spacer) to set the O2k-Stopper into a standard position for a fixed gas phase above the aqueous phase in the O2k-Chamber, during air calibration or for injection of nitrogen, argon or oxygen into the gas phase; Stopper-Spacer thickness of 4mm (a deviation of +/- 10% possible). One Stopper-Spacer is included in the O2k-Assembly Kit (O2k-FluoRespirometer), two in the O2k-TPP+ ISE-Module as standard components.24420-01
Gas spacer for Stopper.jpg
Forceps for membrane applicationForceps for membrane application: for OroboPOS and ISE membrane application; do not use for tissue preparation. This item is a standard component of the OroboPOS-Service Kit and the O2k-TPP+ ISE-Module.26700-01
Forcep for membrane application.jpg
TIP2k-Needle Safety Support with cable guideTIP2k-Needle Safety Support with cable guide: for safe storage of TIP2k-needles and MultiSensor Modules calbes when not required during the experiment. This item is a standard component of the TIP2k-Module, O2k-TPP+ ISE-Module, O2k-pH ISE-Module.31330-02
31330-01 3.jpg
ISE-Service BoxISE-Service Box: for storage and shipment of the O2k-TPP+ ISE-Module or O2k-pH ISE-Module.42001-01
Stopper\black PEEK\angular Shaft\side+6.2+2.6 mm PortStopper\black PEEK\angular Shaft\side+6.2+2.6 mm Port, for application with ISE; side titration port and two additional holes (6.2 mm and 2.6 mm); angular bottom; including Volume-Calibration Ring (A or B); 2 mounted O-rings, with 8 spare O-rings (O-ring\Viton\12.5x1 mm). Two stoppers are included in O2k-TPP+ ISE-Module.42100-01
Stopper black PEEK angular Shaft side+6.2+2.6 mm Port.JPG
Stopper-NeedleStopper-Needle: Short needle for bubble extrusion from port of the ISE-stopper.42120-01
Ion-Selective Electrode TPP+ and Ca2+Ion-Selective Electrode TPP+ and Ca2+: ISE with 6 mm outer diameter shaft, for Stopper\white PVDF\angular Shaft\side+6.2+2.6 mm Port. O2k-TPP+ ISE-Module: 2 ISE.42200-01
Ion-Selective Electrode TPP+ and Ca2+.JPG
ISE-Membrane SealISE-Membrane Seal for Ion-Selective Electrode TPP+ and Ca2+.42213-01
ISE-Membrane Seal.JPG
ISE-Compressible TubeISE-Compressible Tube for Ion-Selective Electrode TPP+ and Ca2+.42214-01
ISE-Compressible Tube.JPG
ISE-Consumables Spares SetISE-Consumables Spares Set42250-01
ISE-Consumables Spares Set.JPG
ISE-TPP+ MembranesISE-TPP+ Membranes, PVC, 4 mm diameter, box of 5 membranes. O2k-TPP+ ISE-Module: 2 boxes per electrode.42270-01
ISE-TPP+ Membranes.JPG
ISE-Membrane Mounting ToolISE-Membrane Mounting Tool for Ion-Selective Electrode TPP+ and Ca2+. O2k-TPP+ ISE-Module: mounting tool included.42300-01
ISA-Membrane Mounting Tool.JPG
ISE-Filling SyringeISE-Filling Syringe with needle for Ion-Selective Electrode TPP+ and Ca2+.42310-01
ISE-Filling Syringe.JPG
Reference-Electrode\2.4 mmReference-Electrode\2.4 mm: 2.4 mm diameter glass shaft, for ISE.42400-01
Reference Electrode 2.4 mm.jpg
Replacement-BarrelReplacement-Barrel for Reference-Electrode\2.4 mm, 2.4 mm diameter glass.42401-01
Electrolyte\Reference-ElectrodeElectrolyte\Reference-Electrode for Reference-Electrode\2.4 mm42402-01
Electrolyte Reference-Electrode.jpg


» O2k-Manual: MiPNet15.03 O2k-MultiSensor-ISE
» O2k-Procedures: TPP: O2k-technical_support
» O2k-Specifications: MiPNet15.08 TPP electrode


Click to expand or collaps
» Keywords: Force and membrane potential
Fundamental relationships
» Force
» Affinity
» Flux
» Advancement
» Advancement per volume
» Stoichiometric number
mt-Membrane potential and protonmotive force
» Protonmotive force
» Mitochondrial membrane potential
» Chemical potential
» Faraday constant
» Format
» Uncoupler
» O2k-Catalogue: O2k-TPP+ ISE-Module
» O2k-Manual: MiPNet15.03 O2k-MultiSensor-ISE
» TPP - O2k-Procedures: Tetraphenylphosphonium
» Specifications: MiPNet15.08 TPP electrode
» Poster
» Unspecific binding of TPP+
» TPP+ inhibitory effect
» O2k-Catalogue: O2k-FluoRespirometer
» O2k-Manual: MiPNet22.11 O2k-FluoRespirometer manual
» Safranin - O2k-Procedures: MiPNet20.13 Safranin mt-membranepotential / Safranin
» TMRM - O2k-Procedures: TMRM
» O2k-Publications: mt-Membrane potential
» O2k-Publications: Coupling efficiency;uncoupling

MitoPedia O2k and high-resolution respirometry: O2k hardware