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The MitoPedia terminology is developed continuously in the spirit of Gentle Science.
- In the Library of SUIT protocols
- » MitoPedia: SUIT - SUIT concept and a list of all SUIT protocols in the library of SUIT protocols.
- »A: MitoPedia: SUIT A - you are here: these are SUIT protocols used for cohort studies.
- »B: MitoPedia: SUIT B - these are published SUIT protocols considered less suitable for cohort studies.
- »C: MitoPedia: SUIT C - these are SUIT protocols used for exploratory research.
- See also:
- » O2k-Protocols: O2k-Demo experiments
- In the Library of SUIT protocols
Library of SUIT protocols: SUIT A
SUIT code | SUIT number | SUIT category | Coupling/pathway control diagram | O2k-Application | Reference |
---|---|---|---|---|---|
SUIT-001 O2 ce-pce D003 | D003_ce1;1Dig;1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11Azd | RP1 ce-pce | O2 | A - SUIT-001 - SUIT reference protocol Reference protocol RP1 for living cells (ce, ROUTINE respiration) and cells permeabilized in the respirometric chamber (pce) | |
SUIT-001 O2 ce-pce D004 | D004_ce1;1Dig;1PM;2D;2c;3U;4G;5S;(6Oct);7Rot;8Gp;9Ama;10AsTm;11Azd | RP1 ce-pce blood | O2 | A - SUIT-001 - SUIT reference protocol Reference protocol RP1 for living PBMC and PLT cells (ce, ROUTINE respiration) and cells that are permeabilized in the chamber (pce) | |
SUIT-001 O2 mt D001 | D001_mt;1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11Azd | RP1 mt | O2 | SUIT-001 - SUIT reference protocol Reference protocol RP1 for isolated mitochondria, tissue homogenate and permeabilized cells | |
SUIT-001 O2 pfi D002 | D002_pfi;1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11Azd | RP1 pfi | O2 | SUIT-001 - SUIT reference protocol Reference protocol RP1 for permeabilized fibers | |
SUIT-002 O2 ce-pce D007 | D007_ce1;1Dig;1D;2M;3Oct;3c;4M;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd | RP2 ce-pce | O2 | A - SUIT-002 - SUIT reference protocol Reference protocol RP2 for living cells (ce, ROUTINE respiration) and cells that are permeabilized in the chamber (pce) | |
SUIT-002 O2 ce-pce D007a | D007a_ce1;1Dig;1D;2M;3Oct;3c;4M;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd | RP2 ce-pce blood | O2 | A - SUIT-002 - SUIT reference protocol Reference protocol RP2 for for living PBMC and PLT cells (ce, ROUTINE respiration) and cells that are permeabilized in the chamber (pce) | |
SUIT-002 O2 mt D005 | D005_mt;1D;2M;3Oct;3c;4M;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd | RP2 mt | O2 | A - SUIT-002 - SUIT reference protocol Reference protocol RP2 for isolated mitochondria, tissue homogenate and permeabilized cells | |
SUIT-002 O2 pfi D006 | D006_pfi;1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd | RP2 pfi | O2 | A -SUIT-002 - SUIT reference protocol Reference protocol RP2 for permeabilized fibers (pfi) | |
SUIT-003 AmR ce D058 | D058_ce1;ce1SOD;ce1HRP;ce1AmR;ce2Omy;ce3U;ce4Rot;ce5Ama | AmR effect on ce | AmR | B: Coupling control in intact cells with oligomycin to study the inhibitory effect of the AmR assay components on the respiration ; ce: non-permeabilized cells -SUIT-003 | |
SUIT-003 O2 ce D009 | D009_ce1;ce2Omy;ce3U;ce4Ama | CCP-ce short | O2 | A: - SUIT-003 - Coupling-control protocol (CCP) in living cells (ce) with oligomycin (LEAK state) | |
SUIT-003 O2 ce D037 | D037_ce1;ce1Glc;(ce2Omy);ce3U;ce4Ama | CCP-ce Crabtree_R | O2 | A SUIT-003 - Coupling-control protocol and Crabtree effect | |
SUIT-003 O2 ce D038 | D038_ce1;(ce2Omy);ce3U;ce3Glc;ce3'U;ce4Ama | CCP-ce Crabtree_E | O2 | A Coupling-control protocol and Crabtree effect test -SUIT-003 complex cell coupling-control protocol | |
SUIT-003 O2 ce D039 | D039_ce1;(ce2Omy);ce3U;ce4Rot;ce5Ama | CCP-ce microalgae | O2 | A: Coupling control in intact cells without oligomycin; ce: non-permeabilized cells -SUIT-003 | |
SUIT-003 O2 ce D050 | D050_ce1;ce1Snv;(ce2Omy);ce3U;ce4Rot;ce5Ama | CCP-ce Snv | O2 | A: - Coupling-control protocol for living cells with plasma membrane-permeable succinate (MitoKit-CII/Succinate-nv) - SUIT-003 - Plasma membrane-permeable succinate (NV118) in ROUTINE respiration. Coupling-control protocol (CCP) in living cells (ce) | |
SUIT-003 O2 ce-pce D020 | D020_ce1;ce1P;ce2Omy;ce3U;ce4Rot;ce5S;1Dig;1c;2Ama;3AsTm;4Azd | CCVP | O2 | A SUIT-003 - Coupling-control protocol and respirometric cell viability test; ce: living cells, pce: permeabilized cells | |
SUIT-003 pH ce D067 | D067_ce1;ce1Glc;ce2Omy;ce3DG;ce4Ama | CCP-Crabtree with glycolysis inhibition | O2 | A Coupling-control protocol and Crabtree effect with glycolysis inhibition- SUIT-003 | |
SUIT-004 O2 pfi D010 | D010_1PM;2D;2c;3U;4S;5Rot;6Ama;7AsTm;8Azd | RP1-short pfi | O2 | SUIT-004 - Short protocol linked to SUIT-001 O2 pfi D002 SUIT RP1 (human skeletal muscle) | |
SUIT-005 O2 pfi D011 | D011_1OctM;2D;2c;3P;4S;5U;6Rot;7Ama;8AsTm;9Azd | RP2-short pfi | O2 | A: - SUIT-005 short protocol linked to SUIT-002 O2 pfi D006 - SUIT RP2 (human skeletal muscle) | |
SUIT-006 AmR mt D048 | D048_1PM;2D;3Omy;4U;5Ama | CCP mt PM - AmR | AmR | A: short protocol for simultaneous determination of O2 flux and H2O2 in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells)- SUIT-006 | |
SUIT-006 Fluo mt D034 | D034_1PM;2D;3Omy;4U;5Ama | CCP mt PM - Fluo | Fluo | A: short protocol for simultaneous determination of O2 flux and mitochondrial membrane potential in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells) -SUIT-006 | |
SUIT-006 MgG ce-pce D085 | D085_ce1;1Dig;1PM;2D;3Cat;4U;5Ama | CCP MgG ce-pce | MgG | A: short coupling-control protocol for simultaneous determination of O2 flux and mitochondrial ATP production in permeabilized cells -SUIT-006 | |
SUIT-006 MgG mt D055 | D055_1PM;2D;3Cat;4U;5Ama | CCP MgG mt | MgG | A: short coupling-control protocol for simultaneous determination of O2 flux and mitochondrial ATP production in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells) -SUIT-006 | |
SUIT-006 O2 ce-pce D029 | D029_ce1;1Dig;1PM;2D;2c;3Omy;4U;5Ama | CCP ce-pce PM | O2 | A to analyse the coupling control on permeabilized cells in N(PM) pathway control state- SUIT-006 | |
SUIT-006 O2 mt D022 | D022_1SRot;2D;2c;(3Omy);4U;5Ama | CCP mt S(Rot) | O2 | A - SUIT-006 - Protocol for coupling control assessment in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells) | |
SUIT-006 O2 mt D047 | D047_1PM;2D;2c;3Omy;4U;5Ama | CCP mt PM | O2 | A SUIT-006 - is a coupling-control protocol in the N-pathway for isolated mitochondria, tissue homogenate and cells permeabilized before addition to the O2k chamber. | |
SUIT-006 Q ce-pce D073 | D073_ce1;1Dig;1Q2;1Rot;1S;2D;(3Omy);4U;5Anox;6Ama.png | CCP ce-pce S(Rot) | Q | A Coupling control protocol to analyze mitochondrial O2 consumption and the redox state of the Q-pool in S(Rot)-pathway control state in permeabilized cells- SUIT-006 | |
SUIT-006 Q mt D071 | D071_mt;1Q2;1Rot;1S;2D;(3Omy);4U;5Anox;6Ama.png | CCP mt S(Rot) | Q | A Coupling-control protocol to analyze mitochondrial O2 consumption and the redox state of the Q-pool in S(Rot)-pathway control state in isolated mitochondria- SUIT-006 | |
SUIT-007 O2 ce-pce D030 | D030_ce1;1Dig;1G;2D;2c;3M;4U;5Ama | Glutamate anaplerotic pathway | O2 | A pce: permeabilized cells - SUIT-007 | |
SUIT-008 O2 ce-pce D025 | D025_ce1;1Dig;1PM;2D;2c;3G;4S;5U;6Rot;7Ama;8AsTm;9Azd | Q-junction ce-pce | O2 | A: for cells-permeabilized cells Lemieux 2017 Sci Rep - SUIT-008 | |
SUIT-008 O2 mt D026 | D026_1PM;2D;2c;3G;4S;4D;5U;6Rot;7Ama;8AsTm;9Azd | Q-junction mtprep | O2 | A: SUIT protocol for mt; mt: isolated mitochondria and tissue homogenate - SUIT-008 | |
SUIT-008 O2 pfi D014 | D014_1PM;2D;2c;3G;4S;4D;5U;6Rot;7Ama;8AsTm;9Azd | O2 | A - Lemieux 2017 Sci Rep - SUIT-008- O2 pfi D014 protocol was used a a reference protocol in MitoEAGLE project (WG2) to evaluate the researcher skills preparing permeabilized fibers | ||
SUIT-010 O2 ce-pce D008 | D008_ce1;ce2Rot;ce3S;ce4D;1Dig;1c | Dig titration-pce | O2 | A -SUIT-010 - Optimum digitonin concentration for pce | |
SUIT-011 O2 pfi D024 | D024_1GM;2D;2c;3S;4U;5Rot;6Ama | NS physiological maximum capapcity in fibres | O2 | A pfi: permeabilized fibers - SUIT-011 | |
SUIT-012 O2 ce-pce D052 | D052_ce1;1Dig;1PM;2D;2c;3G;4U;5Ama | N(PGM) | O2 | A Linear coupling control with NADH-linked substrates for pce - SUIT-012 | |
SUIT-012 O2 mt D027 | D027_1PM;2D;2c;3G;4U;5Ama | N CCP mtprep | O2 | A Linear coupling control with NADH-linked substrates for isolated mitochondria, tissue homogenate and permeabilized cells- SUIT-012 | |
SUIT-013 AmR ce D023 | D023_ce1 | O2 dependence of H2O2 production ce | AmR | A SUIT-013 | |
SUIT-014 O2 pfi D042 | D042_1GM;2D;2c;3P;4S;5U;6Rot;7Ama | NS(PGM) | O2 | A SUIT-014 | |
SUIT-015 O2 pti D043 | D043_1OctM;2D;2c;3G;4P;5S;6U;7Rot;8Ama | FNS(Oct,PGM) | O2 | A pti: permeabilized tissue- SUIT-015 | |
SUIT-016 O2 pfi D044 | D044_1OctM;2D;3G;4S;5Rot;6Omy;7U;8Ama | FNS(Oct,GM) | O2 | A pfi: permeabilized fibers- SUIT-016 | |
SUIT-017 O2 mt D046 | D046_1OctM;2D;2c;3G;3c;4S;5U;6Rot;7Ama | FNS(Oct,GM) | O2 | A: SUIT-017 | |
SUIT-017 O2 pfi D049 | D049_1OctM;2D;2c;3G;4S;5U;6Rot;7Ama | FNS(Oct,GM) | O2 | A: SUIT-017 | |
SUIT-018 AmR ce-pce D068 | D068_ce1;1Dig;1GMS;2D;3Ama | O2 dependence-H2O2 | AmR | A -SUIT-018 - SUIT protocol for simultaneous determination of O2 and H2O2 flux in permeabilized cells at various oxygen concentrations | |
SUIT-018 AmR mt D031 | D031_mt;1GMS;2D;3Ama | O2 dependence-H2O2 | AmR | A -SUIT-018 - SUIT protocol for simultaneous determination of O2 and H2O2 flux in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells) at low oxygen concentration (tissue normoxia) | |
SUIT-018 AmR mt D040 | D040_mt;1GMS;2D;3Ama | NS(GM) | AmR | A: simultaneous determination of O2 and H2O2 flux in mitochondrial preparations (isolated mitochondria, tissue homogenate and permeabilized cells)-SUIT-018 | |
SUIT-018 AmR mt D041 | D041_mt;1GMS;2D;3Ama | O2 dependence-H2O2 | AmR | A -SUIT-018 | |
SUIT-019 O2 pfi D045 | D045_1PalM;2D;2c;3Oct;4P;5G;6U;7S;8Rot;9Ama | FNS(PalOct,PGM) | O2 | A SUIT-019 | |
SUIT-020 Fluo mt D033 | D033_1PM;2D;3G;4S;5Rot;6Omy;7U;8Ama | NS(PGM) | Fluo | A:short protocol for simultaneous determination of O2 flux and mitochondrial membrane potential in mitochondrial preparations (isolated mitochondria, permeabilized cells, and tissue homogenate)- SUIT-020 | |
SUIT-020 O2 mt D032 | D032_1PM;2D;2c;3G;4S;5Rot;6Omy;7U;8Ama | Q-junction additivity and respiratory control for membrane potential | O2 | A: short protocol for determination of O2 flux in mitochondrial preparations (isolated mitochondria, permeabilized cells, and tissue homogenate)-SUIT-020 | |
SUIT-021 Fluo mt D036 | D036_1GM;2D;3S;4Rot;5Omy;6U;7Ama | NS(GM) | Fluo | A: short protocol for simultaneous determination of O2 flux and mitochondrial membrane potential in mitochondrial preparations (isolated mitochondria, permeabilized cells, and tissue homogenate)-SUIT-021 | |
SUIT-021 O2 mt D035 | D035_1GM;2D;2c;3S;4Rot;5Omy;6U;7Ama | NS(GM) | O2 | A:short protocol for simultaneous determination of O2 flux and mitochondrial membrane potential in mitochondrial preparations (isolated mitochondria, permabilized cells, and tissue homogenate)-SUIT-021 | |
SUIT-022 O2 ce D051 | D051_ce1;ce2KCN;ce3SHAM | AOX-ce CN+SHAM | O2 | A Determination of the respiration due to the alternative oxidase pathway -SUIT-022 | |
SUIT-023 O2 ce D053 | D053_ce1;ce2SHAM;ce3KCN | AOX-ce SHAM+CN | O2 | A Determination of the respiration due to the cytochrome c oxidase pathway -SUIT-023 | |
SUIT-024 O2 ce-pce D056 | D056_ce1;1Dig;1PM;2T;2D;3Omy;4Ama | N(PM) | O2 | Determination of the presence of ATPases in mitochondrial preparations - SUIT-024 | |
SUIT-025 O2 mt D057 | D057_1D;2M;3Oct;3c;4M;5P;6G;7S;8Rot;9Ama | FNS(Oct,PGM) | O2 | - SUIT-025 | |
SUIT-026 AmR ce-pce D087 | D087_ce1;1Dig;1S;2Rot;3D;4Ama | RET | AmR | A - SUIT-026 - Protocol for the evaluation of ROS production by RET in permeabilized cells | |
SUIT-026 AmR mt D064 | D064_1S;2Rot;3D;4Ama | RET | AmR | A - SUIT-026 - Protocol for the evaluation of ROS production by RET in mtprep | |
SUIT-026 AmR mt D077 | D077_1S;2Rot;3D;4Ama | RET | AmR | A - SUIT-026 - Protocol for the evaluation of ROS production by RET in mtprep | |
SUIT-027 O2 ce-pce D065 | D065_ce1;1Dig;1M;2D;3M;4P;5G | Malate anaplerosis | O2 | A - SUIT-027 - Protocol for analysis of the malate anaplerotic pathway | |
SUIT-029 O2 mt D066 | D066_mt;1PM;2T;2D;2c;3Omy;4U;5G;6S;6U;7Rot;8Ama | QC_imt_PM_T+OXPHOS+c+Omy_ET_G+S+Rot | O2 | A: quality control evaluation of mitochondrial preparations - SUIT-029 | |
SUIT-031 O2 ce-pce D079 | D079_ce1;1Dig;1PM;2D;2c;3S;4Rot;5U;6Ama | PM+S+Rot | O2 | A to analyse O2 flux in the NS- pathway control state in permeabilized cells- SUIT-031 | |
SUIT-031 O2 mt D075 | D075_1mt;1PM;2D;2c;3S;4Rot;5U;6Ama | PM+S+Rot | O2 | A to analyse O2 flux in the NS- pathway control state on isolated mitochondria- SUIT-031 | |
SUIT-031 Q ce-pce D074 | D074_ce1;1Dig;1Q2;1PM;2D;3S;4Rot;5U;6Anoxia;7Ama | PM+S+Rot | Q | A to analyse O2 flux and the Q-redox state in the NS- pathway control state on permeabilized cells- SUIT-031 | |
SUIT-031 Q mt D072 | D072_mt;1Q2;1PM;2D;3S;4Rot;5U;6Anox;7Ama | PM+S+Rot | Q | A to analyse O2 flux and the Q-redox state in the N-,NS- and S- pathway control state on isolated mitochondria- SUIT-031 |