Mitochondrial preparations

From Bioblast

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Mitochondrial preparations


Mitochondrial preparations (mtprep) are isolated mitochondria (imt), tissue homogenate (thom), mechanically or chemically permeabilized tissue (permeabilized fibers, pfi) or permeabilized cells (pce). In mtprep the plasma membranes are either removed (imt) or mechanically (thom) and chemically permeabilized (pfi), while mitochondrial functional integrity and to a large extent mt-structure are maintained in incubation media optimized to support mitochondrial physiological performance. According to this definition, submitochondrial particles (smtp) are not a mtprep, since mitochondrial structure is altered although specific mitochondrial functions are preserved.

Abbreviation: mtprep

Communicated by Gnaiger E 2011-11-15 (last edit 2022-12-11).

MitoPedia: mt-preparations

Β» Compare: Submitochondrial particles, smtp
O2k-Protocols for mt-preparations: O2k-mitochondrial preparations


Bioblast linkReferenceYear
Gnaiger E et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1.


Preparation: Isolated mitochondria, Permeabilized tissue, Permeabilized cells, Homogenate 

MitoPedia topics: Sample preparation 

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