Difference between revisions of "MitoEAGLE protocols, terminology, documentation"
From Bioblast
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== WG1 Task groups == | == WG1 Task groups == | ||
:: TG leaders: suggestions discussed at the [[MitoFit Science Camp 2016 Kuehtai AT]]. | |||
:: TG participants: listed as a summary from the feedback received. | |||
=== TG1.1 Coupling states and coupling control ratios: Consensus statements === | === TG1.1 Coupling states and coupling control ratios: Consensus statements === |
Revision as of 13:29, 22 August 2016
News and Events | Working Groups | Short-Term Scientific Missions | Management Committee | Members |
COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
MitoEAGLE protocols, terminology, documentation
WG1
- Standard operating procedures and user requirement document: Protocols, terminology, documentation
Project application
WG1 Description
- The quality management system, scope of SOPs to be elaborated and the outline of the data repositories will be defined in a user requirement document.
Respirometric reference protocols
- A ‘library of protocols’ applied in mitochondrial respiratory physiology will be collected in a standard format, delineating the diversity of experimental approaches to assess bioenergetic function. A set of reference protocols will rigorously document (Maelstrom Research program) the state-of-the-art standards in designing, conducting, reporting, interpreting, and validating such bioenergetic tests (see also figure on the right).
Interlaboratory proficiency test
- Inter-laboratory ring tests are a basic requirement for quality management. Such proficiency tests require a reference sample which must be homogenous, stable, representative of the diagnostic target, amenable for distribution, and economical for large-scale production. For respiratory OXPHOS analysis a reference sample of structurally and functionally intact mitochondria is not yet available to perform corresponding ring tests (in contrast to enzymatic OXPHOS ring tests). Recently, the widely applied human cell line HEK 293T was found to be potentially suitable for cryopreservation. Upon thawing the cells can be used immediately for respirometry of both intact and permeabilized cells. Thus interlaboratory proficiency testing may now be feasible as a world-wide innovation in the field of mitochondrial respiratory physiology. Participating labs may implement the test for intra- and interlaboratory validation and longitudinal performance monitoring, using a reference OXPHOS protocol (Fig. 3). The organization of the proficiency test should follow the requirements of the ISO 17043 and 13528 standards. A corresponding MITOEAGLE proficiency training shall be developed and implemented. Feedback from the participants will lead to a final adjustment of the SOPs in the proficiency test, which will be made generally available.
Instrumental platforms
- Comparison of results between and within instrumental platforms: The reference protocols and proficiency test will not define the instrumental platform, but will allow a quantitative comparison of results obtained with specific equipment available in the participating laboratories.
Nomenclature
- Harmonization of nomenclature on mitochondrial respiratory states and control parameters: The logistics of development of a database requires application of strictly defined terms for all included variables. There is no general reference available upon which a consistent terminology on mitochondrial physiology and bioenergetics can be based. The MITOEAGLE consortium, therefore, has to accomplish the ambitious goal to unify and simplify the terminology in the field for the purpose of the QMS, which will lead to the development of recommendations for the use of a common terminology in mitochondrial physiological research. A publication will be prepared as an Open Access article which will be a milestone towards a unification of concepts and nomenclature.
Knowledge management system
- With an increase in data volume and complexity comes along the requirement for standardized report formats and digital accessibility of the data. Since such a database is still missing for mitochondrial physiology, the goal of WG1 is to establish an integrated and analytic environment linking mitochondrial respiration with external data (cell physiology, anthropometry, spiroergometry, lifestyle, genomics) in the context of physical fitness evaluation and clinical diagnostics. Databases will be established combining biological and clinical data comprising proprietary and publicly available datasets into one integrated solution. Data import and export functionalities will allow data upload and mapping to corresponding objects in the database, in this way generating valuable data and allowing for project-specific as well as cross-project data mining.
- The ability to formulate meta-studies in our approach is an important success factor: The platform can aggregate the project’s complete knowledge and provides the ability to make diagnostic profiling associated with diseases. In addition to functional data, other data types such as nutritional or environmental factors associated with disease prevention can be identified and aggregated into the MITOEAGLE Knowledge Management System.
- Querying the heterogeneous data types and result representation are the most important requirements of the knowledge platform besides to data integration and modelling. The application will feature different types of visualization techniques for all integrated data types. The database will be developed using the concept of multi-tier client-server architecture. Individual components are separated corresponding to their functionality. An upstream web server handles user requests, an application server performing business logic, and a back-end database system guaranties persistent data storage. All components are additionally separated by means of network security incorporating firewalls and are hosted in a secure environment. The platform administration ensures privacy regarding proprietary data by user rights policies.
- It will be the task of WG1 to continuously collect the input from all other WGs, compare, discuss and align SOPs as far as possible, and to finally develop recommendations for quality control, data reporting and data sharing beyond the published record. In addition, WG1 will develop concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation. Finally, it will provide a summary on strategic dissemination and an education programme for MITOEAGLE. The education programme will ensure that not only presently participating groups will adhere to the set standards but that also groups joining in at later points may smoothly fit into the community and help to further broaden and deepen our understanding on mitochondrial physiology in health and disease.
WG1 Management
Tasks
- Opening of the database to the research public for data search and moderated entry of new datasets.
- Development of interlaboratory proficiency ring test, of recommendation/certification reports and of a MITOEAGLE proficiency training module.
- Sample distribution, ring test experiments (MITOEAGLE-proficiency training on two different points of time, 6 months apart), data analysis by experimenters and MITOEAGLE-proficiency test manager.
- Reports and publication: Reports with specific results in comparison with an anonymous data summary, and joint publication. Individual reports may be complemented by recommendations, and are summarized in an informal certification report.
- Instrumental platform comparison with reference sample applying comparable measurements.
- Draft, dissemination, collection and final formulation of a manuscript on concepts and terminology of mitochondrial physiology.
- Collect and discuss procedures and experimental protocols for the evaluation of mitochondrial capacities and create a library of protocols: A database comprising reference SUIT protocols, standard experimental media, and detailed instructions for OXPHOS analysis is assembled.
- MITOEAGLE data management system (DMS) development: The DMS for further data preprocessing. The DMS will enable management and analysis of instrument-specific and project-specific data.
- MITOEAGLE database (DB) development: DB will facilitate high-level project specific analysis as well as meta-analyses across projects, visualization of project specific data as well as integrative analyses of datasets for various proprietary and/or publicly available datasets.
- Testing of developed software components regarding functionality and usability, modification and implementation. Deployment for routine testing in the labs of our strategic partners.
- Data input: Upload of datasets generated through WG 2-4 in internal lab developments, results of the proficiency ring test, and during application module developments. In addition, the database will be populated with available proprietary and public datasets.
- Develop recommendations for quality control, data reporting and data sharing beyond the published record. Develop concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation.
- Provide a summary on strategic dissemination and an education programme for MITOEAGLE.
Milestones
- Data sets entered in standardized format and database opened for the public
- Protocols for ring-tests and standard analysis and report scheme defined
- MITOEAGLE proficiency training workshop finished
- Analysis is completed and joint publication submitted
- Publication of paper on concepts and terminology
- Library of protocols online
- Publication of recommended protocols and procedures
- Guidelines for acquisition, evaluation and data documentation available
- MITOEAGLE data management system finished
- MITOEAGLE database ready for use
- Software modifications incorporated following extensive tests
- Publication of MITOEAGLE-KMP
- Recommendations on data acquisition, evaluation and management published
- Concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation published
Deliverables
- Guidelines for future research and recommendations for the evaluation of respiratory characteristics in human respirometric samples
- A database on mt fitness evaluated with human cells and tissues
- Training of researchers towards improved reproducibility of sample preparation and respirometric evaluation
- Qualitative and quantitative evaluation of results obtained in ongoing studies by comparison with a reference sample
- An expanded MITOEAGLE database
- A joint publication documenting the results of the MITOEAGLE-PT
- A multi-authored publication towards a unification of concepts and nomenclature in mitochondrial physiology
- A library of protocols for sample preparation and examination by respirometry
- A multi-authored publication with recommendations of comparable standard protocols and procedures.
- Guidelines as to the optimum use of a reference sample for determination of respiration and related parameters of interest.
- The MITOEAGLE data management system.
- A continuously updated MITOEAGLE database for the use as reference data and guideline towards application of optimized protocols, ultimately leading towards standardization.
- A joint publication on the use of the MITOEAGLE database and management system.
- An education programme for the use of MITOEAGLE database and management system.
WG1 Participants and task groups
WG1 Participants
Country | City | Contact |
---|---|---|
Armenia | Yerevan | Trchounian K |
Australia | Melobourne | Botella Javier |
Australia | Queensland | Radenkovic Filip |
Australia | Southport | Vidimce Josif |
Australia | Melbourne | Stepto Nigel K |
Australia | Melbourne | Whitfield Jamie |
Australia | Newtown, Sydney | Stocker Roland |
Australia | Geelong, Victoria | McKenzie Matthew |
Australia | Clayton | Wolff Jonci Nikolai |
Australia | Queensland | Dong Lan-Feng |
Australia | Melbourne, VIC | Muccini Anna Maria |
Australia | Melbourne | Ferri Alessandra |
Australia | Melbourne | Saner Nicholas |
Australia | Nedlands | Perks Kara L |
Australia | Victoria | Van Bergen Nicole J |
Australia | Syndey | Ton Riccardo |
Australia | Burwood | Trewin Adam J |
Australia | Victoria | Winwood-Smith Hugh |
Australia | Melbourne | Kuang Jujiao |
Australia | Melbourne | Hewakapuge Sudinna |
Australia | Parkville | Hardee Justin P |
Australia | Melbourne | Lionett S |
Australia | Clayton Victoria | Alton Lesley |
Australia | Sydney | Hoehn Kyle L |
Australia | Nedlands | Filipovska Aleksandra |
Australia | Melbourne | Cassar Samantha |
Australia | Melbourne | Coughlan Melinda T |
Australia | Melbourne | Genders Amanda J |
Australia | Sydney | Krycer James R |
Australia | Queensland | Holland Olivia J |
Australia | Queensland | Fisher Joshua J |
Austria | Innsbruck | Thapa Maheshwor |
Austria | Innsbruck | Plangger Mario |
Austria | Innsbruck | Sobotka Ondrej |
Austria | Innsbruck | Schartner Melanie |
Austria | Innsbruck | Goebel G |
Austria | Innsbruck | Di Marcello Marco |
Austria | Innsbruck | Bergmeister Lisa |
Austria | Innsbruck | Garcia-Souza Luiz F |
Austria | Innsbruck | Antunes Diana |
Austria | Kapferer Werner | |
Austria | Innsbruck | Klocker Helmut |
Austria | Innsbruck | Meszaros Andras T |
Austria | Innsbruck | Laner Verena |
Austria | Innsbruck | Gallee Leon |
Austria | Salzburg | Kofler B |
Austria | Innsbruck | Karabatsiakis Alexander |
Austria | Innsbruck | Cardoso Luiza HD |
Austria | Innsbruck | Perez Valencia Juan Alberto |
Austria | Innsbruck | Gnaiger Erich |
Austria | Aldrans | Haider Markus |
Austria | Innsbruck | Schmitt Sabine |
Austria | Innsbruck | Schwarzer Christoph |
Austria | Klosterneuburg | Sazanov Leonid A |
Austria | Innsbruck | Cecatto Cristiane |
Austria | Innsbruck | Passrugger Manuela |
Austria | Innsbruck | Burtscher Martin |
Austria | Vienna | Kozlov Andrey V |
Austria | Innsbruck | Nindl Veronika |
Austria | Innsbruck | Hoppel Florian |
Austria | Innsbruck | Keller Markus A |
Austria | Innsbruck | Doerrier Carolina |
Belgium | Leuven, Flanders | Baekelandt V |
Belgium | Namur | Arnould Thierry |
Belgium | Brussels | Sonveaux P |
Belgium | Liege | Votion Dominique-Marie |
Belguim | Sart Tilman (Liège) | Kruse C |
Brasil | Porto Alegre | Haas Clarissa |
Brasilia | Limeira | Ropelle Eduardo R |
Brazil | Sao Paulo | Silber Ariel Mariano |
Brazil | Rio de Janeiro | Galina Antonio |
Brazil | Sao Paulo | Vercesi Anibal E |
Brazil | Santa Maria - RS | Goncalves Debora Farina |
Brazil | Rio de Janeiro | Vieyra Adalberto |
Brazil | Santa Maria - Rio Grande do Sul | Soares Felix Alexandre Antunes |
Brazil | São Paulo | Kowaltowski Alicia J |
Brazil | Jaboticabal | Oliveira Marcos Tulio |
Brazil | Rio de Janeiro | Oliveira Marcus F |
Brazil | São Paulo | Crispim Marcell |
Canada | Edmonton (Alberta) | Lemieux Helene |
Canada | Edmonton | Zaugg Michael |
Canada | Edmonton | Han Woo Hyun |
Canada | Rimouski | Munro Daniel |
Canada | Vancouver, BC | Tausan Daniel |
Canada | Saint John | Pulinilkunnil Thomas |
Canada | Quebec | Joseph Vincent |
Canada | Edmonton | Lucchinetti Eliana |
Canada | Charlottetown | Adiele Reginald C |
Canada | Hamilton | Tarnopolsky Mark A |
Canada | Rimouski | Blier Pierre U |
Canada | Guelph | Holloway Graham P |
Canada | Winnipeg | Chowdhury Subir Roy |
Canada | Ottawa | Harper Mary-Ellen |
Canada | Antigonish | Weir M |
Canada | Ottawa | Darveau Charles-A |
Canada | Vancouver | Bovard Josh |
Canada | Montréal | Breton Sophie |
Canada | Hamilton | Scott Graham R |
Canada | Moncton, NB | Pichaud Nicolas |
Canada | Montreal, Quebec | Bergdahl Andreas |
Chile | Coyhaique | Jana Prado Fabian |
China | Xi`an | Liu Jiankang |
China | Tianjin | Zhang Yong |
China | Pukou, Nanjing, | Gan Zhenji |
China | Fuzhou, Fujian Province | Chen Qi |
Croatia | Split | Ljubkovic M |
Croatia | Zagreb | Saric A |
Croatia | Zagreb | Curik I |
Croatia | Zagreb | Rubelj Ivica |
Czech Republic | Ceske Budejovice | Zikova Alena |
Czech Republic | Pardubice | Handl Jiri |
Czech Republic | Praha 2 | Danhelovska Tereza |
Czech Republic | Hradec Kralove | Stankova Pavla |
Czech Republic | Vestec | Ezrova Zuzana |
Czech Republic | Hradec Kralove | Cervinkova Zuzana |
Czech Republic | Vestec | Rohlena Jakub |
Czech Republic | Praha 2 | Zdrazilova Lucie |
Czech Republic | Prague | Krizova J |
Czech Republic | Pardubice | Rousar Tomas |
Czech Republic | Vestec | Davidova Eliska |
Czech Republic | Prague | Drahota Zdenek |
Czech Republic | Prague | Houstek Josef |
Czech Republic | Prague 10 | Urban Tomas |
Czech Republic | Hradec Kralove | Endlicher Rene |
Czech Republic | Hradec Kralove | Kucera Otto |
Czech Republic | Plzen -Severni predmesti | Jedlicka Jan |
Czech Republic | Pardubice | Majtnerova Pavlina |
Czech rep | Hradec Kralove | Falaye T |
Denmark | Copenhagen | Dela Flemming |
Denmark | Copenhagen | Nielsen B |
Denmark | Hvidovre | Nehlin Jan O |
Denmark | Aarhus N | Boetker Hans Erik |
Denmark | Copenhagen | Larsen Steen |
Denmark | Copenhagen | Soendergaard SD |
Denmark | Copenhagen N | Scheibye-Knudsen Morten |
Denmark | Copenhagen N | Maise Chroeis K |
Denmark | Copenhagen | Gonzalez-Franquesa Alba |
Denmark | Copenhagen | Kraunsoee R |
Denmark | Roskilde | Dalgaard Louise T |
Denmark | Aarhus N | Jespersen Nichlas Riise |
Denmark | Copenhagen N | Mortensen OH |
Egypt | Cairo | Abdel-Rahman Engy Ali |
Egypt | Cairo | Ali Sameh S |
Estonia | Tallinn | Puurand Marju |
Estonia | Tallinn | Kaambre Tuuli |
Estonia | Tallinn | Laasmaa Martin |
Estonia | Tallinn | Klepinin Aleksandr |
Estonia | Tallinn | Karro N |
Estonia | Tallinn | Tepp Kersti |
Estonia | Tallinn | Shevchuk Igor |
Estonia | Tallinn | Truu Laura |
Estonia | Tallinn | Klepinina Lyudmila |
Estonia | Tallinn | Vendelin Marko |
Estonia | Tartu | Paju Kalju |
Finland | Turku | Stier Antoine |
Finland | Helsinki | Jackson Christopher Benjamin |
Finland | Helsinki | Suomalainen Wartiovaara Anu |
Finland | Jyvaeskylae | Kainulainen Heikki |
France | Paris | Bouillaud Frederic |
France | Paris | Armand Anne-Sophie |
France | Plouzané | Salin Karine |
France | Tours | Dumas Jean-Francois |
France | Angers | Procaccio Vincent |
France | Nouzilly | Collin-Chenot Anne |
France | Angers | Spinazzi Marco |
France | Bordeaux cedex | Mourier A |
France | Lille | Montaigne David |
France | Maisons-Alfort Cedex | Prola Alexandre |
France | Montpellier | Wrutniak-Cabello Chantal |
France | Bordeaux cedex | Rossignol Rodrigue |
France | Martinique | Cano Sanchez Maria Consolacion |
France | Bordeaux | Amoedo Nivea Dias |
France | Bordeaux | Sarlak Saharnaz |
France | Martinique | Neviere Remi |
France | Gueguen Naig | |
Germany | Giessen | Sommer Natascha |
Germany | Ulm | Schaefer PM |
Germany | Jena | Szibor Marten |
Germany | Cologne | Maciej Sarah |
Germany | Hannover | Das Anibh Martin |
Germany | Mainz | Methner Axel |
Germany | Munich | Zischka Hans |
Germany | Cologne | Pesta Dominik |
Germany | Frankfurt | Hamann Andrea |
Germany | Magdeburg | Schoenfeld Peter |
Germany | Marburg | Vogt Sebastian |
Germany | Bremerhaven | Mark Felix Christopher |
Germany | Duesseldorf | Piel Sarah |
Germany | Cologne | Wiesner Rudolf J |
Germany | Wickert Anika | |
Germany | Gießen | Silaidos Carmina |
Germany | Regensburg | Renner-Sattler Kathrin |
Germany | Freiburg | Schuele R |
Germany | Cologne | Trifunovic Aleksandra |
Germany | Marburg | Ramzan Rabia |
Germany | Frankfurt am Main | Viel Christian |
Germany | Rostock | Sokolova Inna |
Germany | Gießen | Grewal Rekha |
Germany | Duesseldorf | Haendeler Judith |
Germany | Doermann Niklas | |
Germany | Mainz | Maull Felicia |
Germany | Frankfurt | Osiewacz Heinz D |
Germany | Neuherberg | Einer C |
Germany | Cologne | Ho Dieu Hien |
Germany | Wilhelmshaven | Salmon Pablo |
Germany | Düsseldorf | Goy C |
Germany | Duesseldorf | Roden Michael |
Germany | Biberach an der Riß | Nold V |
Germany | Tuebingen | Kappler Lisa |
Germany | Frankfurt | Warnsmann Verena |
Germany | Giessen | Schulz Rainer |
Germany | Duesseldorf | Jelenik T |
Greece | Thessaloniki | Lazou Antigone |
Greece | 15771 | Andreadou Ioanna |
Greece | Heraklion | Tavernarakis Nektarios |
Greece | Athens | Gumeni Sentiljana |
Greece | Athens | Trougakos Ioannis P |
Hungary | Budapest | Chinopoulos Christos |
Hungary | Szeged | Ferdinandy Peter |
Hungary | Budapest | Tretter Laszlo |
Hungary | Budapest | Komlodi Timea |
Hungary | Szeged | Boros Mihaly |
Hungary | Debrecen | Verebne Tar K |
Hungary | Budapest | Horvath G |
India | Lucknow | Gayen Jiaur |
India | Haryana | Chakrabarti Sasanka |
India | Varanasi | Sonkar Vijay K |
India | Kollam | Suravajhala Prashanth |
India | New Delhi | Thakkar Himani |
India | Hyderabad | Jha Rajan Kumar |
India | New Delhi | Vincent Vinnyfred |
Iran | Tehran | Shirazi Reza |
Iran | Tehran | Safaei Zahra |
Ireland | Dublin | Porter Richard K |
Ireland | Dublin | O'Gorman Donal |
Israel | Zrifin | Sova Marina |
Israel | Beer Sheva | Mishmar Dan |
Israel | Jerusalem | Saada Reisch Ann |
Israel | Rishon Le Zion | Hachmo Yafit |
Italia | Udine | Salvadego Desy |
Italien | Padova PD | Szabo Ildiko |
Italy | Udine | Grassi B |
Italy | Rome | Scatena Roberto |
Italy | Bologna | Paterlini S |
Italy | Milano | Bottoni P |
Italy | Ancona | Battino Maurizio |
Italy | St. Lorenzen | Harrison David K |
Italy | Bologna | Porcelli AM |
Italy | Padova | Viscomi Carlo |
Italy | Padova | Bernardi Paolo |
Italy | Milan | Clementi Emilio |
Italy | Bolzano | Zanon Alessandra |
Italy | Bologna | Gasparre G |
Italy | Bolzano/Bozen | Volani Chiara |
Italy | Monserrato | Isola Raffaella |
Italy | Bari | Attimonelli M |
Italy | Catania | Messina Angela |
Italy | Catania | Magri Andrea |
Italy | Catania | De Pinto Vito |
Italy | Padova | Fernandez-Vizarra Erika |
Italy | Bologna | Genova Maria Luisa |
Italy | Campobasso | Pallotta Maria Luigia |
Japan | Sapporo | Matsumoto J |
Japan | Itabashi-ku Tokyo | Tanaka Masashi |
Kitzbühel | Innsbruck | Fischer Michael J |
Korea | Seoul | Pak Youngmi Kim |
Korea | Incheon | Kwak Hyo Bum |
Korea (South) | Seoul | Lee Hong Kyu |
Latvia | Riga | Svalbe Baiba |
Latvia | Riga | Zvejniece Liga |
Latvia | Riga | Stelfa Gundega |
Latvia | Riga | Liepins Edgars |
Latvia | Riga | Volska Kristine |
Latvia | Riga | Jansone Baiba |
Latvia | Riga | Inashkina I |
Latvia | Riga | Dambrova Maija |
Latvia | Riga | Vilks Karlis |
Luxembourg | Strassen | Devaux Yvan |
Malaysia | Kuala Lumpur | Hassan Hazirah |
Malta | Msida | Vella Joanna |
Mexico | Tlalpan | Rodriguez-Enriquez Sara |
Mexico | Mexico City | Aparicio Trejo OE |
México | San Pedro Garza García, Nuevo Leon | Garcia-Rivas Gerardo |
Netherlands | Maastricht | Grevendonk L |
Netherlands | GA Wageningen | Grefte Sander |
New Zealand | Auckland | Hickey Anthony J |
New Zealand | Auckland | Kaur Sarbjot |
New Zealand | Auckland | Ward Marie Louise |
New Zealand | Wellington | Berridge Michael V |
Norway | Bergen | Hoel Fredrik |
Norway | Bergen | Pettersen Nitschke Ina Katrine |
Norway | Trondheim | Grill V |
Norway | Bergen | Roesland Gro Vatne |
Norway | Bergen | Tronstad Karl Johan |
Norway | Tromsoe | Boardman Neoma T |
Norway | Bergen | Berge Rolf K |
Norway | Trondheim | Smenes Benedikte Therese |
Norway | Bergen | Blindheim Dan Filip |
Norway | Trondheim | Rognmo O |
Norway | Oslo | Eide L |
Norway | Bergen | Haavik Jan |
Norway | Bergen | Dyrstad Sissel E |
Norway | Trondheim | Lerfall Joergen |
Poland | Gdańsk | Kaczor Jan Jacek |
Poland | Warsaw | Sek Aleksandra |
Poland | Warsaw | Karkucinska-Wieckowska Agnieszka |
Poland | Warsaw | Szewczyk Adam |
Poland | Warsaw | Wasilewski M |
Poland | Warsaw | Bednarczyk Piotr |
Poland | Warszawa | Jaskiewicz Anna |
Poland | Lodz | Watala Cezary |
Poland | Warsaw | Kampa Rafal Pawel |
Poland | Warsaw | Zablocki Krzysztof |
Poland | Krakow | Dembinska-Kiec Aldona |
Poland | Warsaw | Wieckowski Mariusz R |
Poland | Lodz | Labieniec-Watala Magdalena |
Poland | Warsaw | Dymkowska Dorota |
Poland | Poznan | Jarmuszkiewicz Wieslawa |
Portugal | Aveiro | Vitorino Rui Miguel Pinheiro |
Portugal | Cantanhede | Amorim Ricardo |
Portugal | Porto | Beleza Jorge |
Portugal | Cantanhede | Teodoro Joao Soeiro |
Portugal | Coimbra | Dias Candida |
Portugal | Coimbra | Santos Diana |
Portugal | Coimbra | Oliveira Paulo J |
Portugal | Porto | Alves Marco G |
Portugal | Coimbra | Duarte Filipe Valente |
Portugal | Coimbra | Grilo Luis |
Portugal | Coimbra | Minuzzi Luciele M |
Portugal | Lisboa | Macedo Maria Paula |
Portugal | Porto | Oliveira Jorge |
Portugal | Coimbra | Rolo Anabela Pinto |
Portugal | Aveiro | Ferreira Rita Maria P |
Portugal | Porto | Oliveira Pedro Fontes |
Portugal | Coimbra | Palmeira Carlos |
Portugal | Cantanhede | Rodrigues Ana Sofia |
Portugal | Porto | Crisostomo Luis |
Portugal | Porto | Moreira Bruno P |
Portugal | Porto | Silva Ana Maria |
Qatar | Doha | Guarch Meritxell Espino |
Republic of Ghana | Cape Coast | Tei BN |
Republic of Korea | Busan | Han Jin |
Republic of Korea | Yuseong-gu Daejeon | Quang Don T |
Republic of Korea | Daejeon | Park SH |
Republic of Korea | Busan | Kim Hyoung Kyu |
Republic of Serbia | Novi Sad | Andric Silvana |
Republic of Serbia | Belgrade | Krako Jakovljevic Nina |
Republic of Serbia | Belgrade | Lalic Nebojsa M |
Russia | Moscow | Vinogradov Andrey D |
Russia | Moscow | Pavlova Nadia |
Russia | Yoshkar-Ola | Dubinin M |
Serbia | Belgrade | Misirkic Marjanovic Maja |
Serbia | Belgrade | Mandic M |
Serbia | Belgrade | Djordjevic M |
Serbia | Belgrade | Savkovic U |
Singapore | Singapore | Hausenloy Derek J |
Singapore | Singapore | Singh Brijesh Kumar |
Slovakia | Košice | Cizmarova Beata |
Slovakia | Bratislava | Sumbalova Zuzana |
Slovakia | Bratislava 45 | Ferko Miroslav |
Spain | Madrid | Moran M |
Spain | A Coruña | Blanco FJ |
Spain | Granada | Hidalgo-Gutierrez A |
Spain | Armilla (Granada) | Fernandez-Ortiz Marisol |
Spain | Barcelona | Perales Jose Carles |
Spain | Valencia | Casado Pinna Marta |
Spain | Barcelona | Gil J |
Spain | Barcelona | Gama Perez Pau |
Spain | Granada | Quiles JL |
Spain | Barcelona | Garcia-Roves Pablo Miguel |
Spain | Madrid | Hernansanz-Agustin Pablo |
Spain | Barcelona | De la Torre Lara J |
Spain | Barcelona | Zorzano Antonio |
Spain | A Coruña | Mayan MD |
Spain | Sevilla | Bustos Matilde |
Spain | Salamanca | Almeida Angeles |
Spain | Las Palmas de Gran Canaria | Calbet Jose AL |
Spain | Madrid | Rial Eduardo |
Spain | Madrid | Aragones Lopez J |
Spain | Barcelona | Goncalo Teixeira da Silva Rui |
Spain | Barcelona | Bravo-Sagua Roberto |
Sweden | Stockholm | Nedergaard J |
Sweden | Stockholm | Larsen Filip J |
Sweden | Stockholm | Shabalina Irina G |
Sweden | Gothenburg | Bhuvanachandran NS |
Sweden | Lund | Ehinger Johannes K |
Sweden | Stockholm | Cardinale Daniele A |
Switzerland | Bern | Wyss RK |
Switzerland | Lausanne | Lagarrigue Sylviane |
Switzerland | Basel | Fornaro Mara |
Switzerland | Zurich | Gorr Thomas A |
Switzerland | Lausanne | Donnelly Chris |
Switzerland | Lausanne | Morato Fornaguera L |
Switzerland | Zürich | Zaugg Kathrin |
Switzerland | Lausanne | Weger M |
Switzerland | Bern | Nuoffer Jean-Marc |
Switzerland | Lausanne | Place Nicolas |
Switzerland | Lausanne | Zanou Nadege |
Switzerland | Lausanne | Amati Francesca |
Switzerland | Lausanne | Zalachoras I |
Switzerland | Lausanne | Burtscher Johannes |
Switzerland | Lausanne | Canto Alvarez Carles |
Switzerland | Basel | Bouitbir Jamal |
Switzerland | Lausanne | Sandi Carmen |
Switzerland | Lausanne | Gebara E |
Taiwan | Changhua City | Wei Yau-Huei |
The Netherlands | Maastricht | Nabben Miranda |
The Netherlands | Delft | McMillan Duncan GG |
The Netherlands | Groningen | Bakker Barbara M |
The Netherlands | Maastricht | Schrauwen Patrick |
The Netherlands | BT Amsterdam | Wuest Rob CI |
UK | Canterbury, Kent | Gourlay Campbell W |
UK | London | Lane Nick |
UK | London | Bettinazzi Stefano |
UK | London | Rodriguez Enrique |
UK | Cambridge | Brown Guy C |
USA | Kansas City | Thyfault John P |
USA | Fort Collins | Chicco Adam J |
USA | Torrance | Rossiter Harry B |
USA | Little Rock | Parajuli Nirmala |
USA | La Jolla | Valentine Joseph Marco |
USA | Needham | Brown David A |
USA | Indianapolis | Brozinick Joseph T |
USA | Winston-Salem | Gonzalez-Armenta Jenny L |
USA | New York | Galkin Alexander |
USA | Boston | Kristal Bruce S |
USA | Bethesda | Fessel Joshua Patrick |
USA | Atlanta | Jang Young Charles |
USA | Louisville | Skolik Robert A |
USA | Iowa City | Chaurasia Bhagirath |
USA | Fairbanks | O'Brien Kristin |
USA | Winston-Salem | Ahn Bumsoo |
USA | San Diego | Patel Hemal H |
USA | Fort Collins | Li Puma Lance C |
USA | Philadelphia | Towheed Atif |
USA | St. Louis | Abumrad Nada A |
USA | Wakefield | Varricchio Frederick |
USA | Cleveland | Hoppel Charles L |
USA | Baltimore | Gonzalez-Freire Marta |
USA | Stillwater | Davis Michael S |
USA | Corvallis | Newsom Sean A |
USA | Birmingham | Moellering Douglas R |
USA | Orlando | Sparks Lauren M |
USA | Winston-Salem | Tomar Dhanendra |
USA | Rochester | Nair K Sreekumaran |
USA | East Lansing | Bazil Jason N |
USA | Philadelphia | Ganetzky Rebecca |
USA | Pittsburgh | Jurczak Michael J |
USA | Chicago | Dayanidhi Sudarshan |
USA | San Diego; La Jolla | Molina Anthony JA |
USA | Gainesville | Leeuwenburgh Christiaan |
USA | Akron | Cohen Bruce H |
USA | East Lansing | Zhang Yizhu |
USA | Davis | Hellgren Kim T |
USA | Corvallis | Batterson Philip M |
USA | Los Angeles | Kwast Kurt E |
USA | Davis | Adams Sean H |
USA | Provo | Hancock Chad R |
USA | St. Louis | Pietka Terri A |
USA | Norfolk | Lai Nicola |
USA | Philadelphia | Jang David H |
USA | Aurora | Ford Ellen |
USA | Ann Arbor | Tyrrell Daniel J |
USA | San Diego | Cannon Daniel T |
USA | Pittsburgh | Prochownik Edward V |
USA | Rochester | Lanza Ian R |
USA | Galveston | Durham William J |
USA | Fayetteville | Rao RR |
USA | Omaha | Kim Julian KS |
USA | Milwaukee | Dash Ranjan K |
USA | Kansas City | Swerdlow Russell H |
USA | Colorado Springs | Jacobs RA |
USA | Nashville | Wasserman David H |
USA | Bethesda | Sharma Pushpa |
USA | Philadelphia | Falk Marni J |
USA | Denton | Dzialowski Edward M |
USA | Iowa City | Wagner Brett A |
USA | Fayetteville | Iyer Shilpa |
USA | Portland | Garlid Keith D |
USA | Orlando | Coen Paul M |
USA | Orlando | Goodpaster Bret H |
USA | Jupiter | Trivigno Catherine |
USA | Baton Rouge | Irving Brian A |
USA | Denver | Van Hove Johan |
USA | Aurora | Sparagna Genevieve C |
USA | El Paso | Bajpeyi Sudip |
USA | Stanford | Stary Creed |
USA | Durham | Li Pingan Andy |
USA | Baton Rouge | Hand Steven C |
USA | Fairbanks | Coker Robert H |
USA | Warminster | Orynbayeva Zulfiya |
USA | Corvallis | Robinson Matthew M |
USA | Columbus | Sims Carrie A |
USA | Little Rock | Borsheim Elisabet |
USA | Cambridge | Wohlwend Martin |
USA | Little Rock | MacMillan-Crow Lee Ann |
USA | Gainesville | Neyroud D |
USA | Greenville | Neufer P Darrell |
USA | Orlando | Pino Maria F |
USA | La Jolla | Schilling Jan M |
... further results |
WG1 Task groups
- TG leaders: suggestions discussed at the MitoFit Science Camp 2016 Kuehtai AT.
- TG participants: listed as a summary from the feedback received.
TG1.1 Coupling states and coupling control ratios: Consensus statements
- TG leaders: Renner-Sattler Kathrin / Gnaiger Erich / Hoppel Charles L
- TG participants:
TG1.2 SUIT protocols: nomenclature consistent with concept of database
- TG leader: Gnaiger Erich
- TG participants:
TG1.3 Experimental details in SUIT
- TG leaders: Chicco Adam J / Hickey Anthony J / Doerrier Velasco Carolina A
- TG participants: Casado Pinna Marta
TG1.4 Instrumental platforms: comparisons
- TG leaders: Molina Anthony JA
- TG participants: Canto Carles (suggested), Genova Maria Luisa, Tronstad Karl Johan (suggested), Zorzano Antonio (suggested)
TG1.5 Knowledge management system
- TG leaders: Drinnan Michael / Plattner Christina
- TG participants:
TG1.6 Proficiency test
- TG leaders: Gnaiger Erich / Doerrier Velasco Carolina A
- TG participants: Genova Maria Luisa, Plattner Christina
TG1.7 Documentation
- TG leaders: Arnould Thierry / Votion Dominique-Marie
- TG participants:
TG1.8 Translation to community in general
- TG leader: Irving Brian A
- TG participants:
TG1.9 Dissemination Scientific community (formally WG5)
- TG leaders: Gnaiger Erich /Makrecka-Kuka Marina
- TG participants: Ali Sameh S, Cervinkova Zuzana, Chicco Adam J, Coen Paul M, Eide Lars, Garcia-Roves Pablo M, Gasparre Giuseppe, Genova Maria Luisa, Haendeler Judith, Iyer Shilpa, Mayan Maria D, Mishmar Dan, Nehlin Jan, Palmeira Carlos, Paterlini Serena, Porcelli Anna Maria, Rao Raj, Schaefer Patrick, Tronstad Karl Johan, Trougakos Ioannis, Volani Chiara, Votion Dominique-Marie
TG1.10 Training, Short Term Scientific Missions (formally WG5)
- TG leader: Klaus Susanne (suggested), Labieniec-Watala Magdalena (suggested)
- TG participants: Ali Sameh S, Cervinkova Zuzana, Chicco Adam J, Coen Paul M, Eide Lars, Garcia-Roves Pablo M, Gasparre Giuseppe, Genova Maria Luisa, Haendeler Judith, Iyer Shilpa, Mayan Maria D, Mishmar Dan, Nehlin Jan, Palmeira Carlos, Paterlini Serena, Porcelli Anna Maria, Rao Raj, Schaefer Patrick, Tronstad Karl Johan, Trougakos Ioannis, Volani Chiara, Votion Dominique-Marie
WG1 Comments and discussion
- We are mostly interested in two WG: (1) Since we are working mostly with heart and muscles, we are interested in WG 2 - MITOEAGLE data repository in muscle and other tissues. (2) WG 1 - Standard operating procedures and user requirement document: Protocols, terminology, documentation. Since we have an expertise in fatty acid metabolism, we are specially interested in experimental protocols for the evaluation of mitochondrial capacities regarding fatty acid metabolism. Of course our parts of the WG are also in our interest. - Marina Makrecka-Kuka, Edgars Liepinsh (2016).
- My role in Mito-EAGLE would be mainly in WG 2 and 4, with a focus on skeletal and cardiac muscle and cultured cardiomyocytes and endothelial cells. I would particularly like to bridge the gap between the group leaders and young investigators, trying to represent the young people who are the active users of the OROBOROS equipment. This will fit in nicely with the goals in WG1. ... Major congratulations on obtaining this beautiful collaborative grant! Very glad to see this network getting the recognition it deserves! Looking forward to being part of this! - Rob C Wuest (2016).
- Please, find enclosed my declaration to participate with my team in the following workgroups: WG1, WG2, WG3, WG4. We are now involved and have been in not a distant past – in studying of the following cell /tissue types:
- Blood cells, with particular emphasis on platelets and lymphocytes – isolated from animals and humans.
- Endothelial cells, fibroblasts, neurocytes (neuroblastoma), astrocytes, cancer cell lines – predominantly cultured.
- Hepatocytes, cardiomyocytes, cell isolated from brain – isolated from lab animals. - Cezary Watala (2016).
- I have already contacted the portuguese CNC to nominate me for the MC of theaction. Concerning my active participation, taking into account the type of work we do in my lab I could be included in all WGs. As you know we work with mitochondria from liver, muscle and fat, as well as cultured cells from different origins (WG2-4). The elaboration of SOPs (WG1) we can certainly contribute (see the book I edited on Mitochondrial Bioenergetics and in which you contribute a chapter). I believe that everybody can be part of the WG5, but as the coordinators of the action, you and Sandra should decide what is best for the success of the action. - Carlos Palmeira (2016).
- I would propose my participation in the WG1: Standard operating procedures and user requirement document: Protocols, terminology, documentation, considering that the goal of WG1 is to establish an integrated and analytic environment linking mitochondrial respiration with external data (cell physiology, anthropometry, spiroergometry, lifestyle, genomics) in the context of physical fitness evaluation and clinical diagnostics. I could give my active contribution in the phase of making platform that will aggregate the Action’s complete knowledge and provides the ability to make diagnostic profiling associated with diseases in particular tasks:
- Develop reccomendations for quality control, data reporting and data sharing beyond the published record. Develop concepts on Open Access and institutionalised service for data management, data mining and health-care conforming standards of data interpretation
- Provide a summary on strategic dissemination and an education programme for MITOEAGLE.
- Considering that we are in process of buying the oxygraph, thus broading capacities of my lab, I would be happy to open the door of my new lab for the innovative inter-laboratory ring test experiments and together with my team contribute in that phase of the MITOEAGLE Action. I look forward to the establishment of MitoGlobal EAGLE network where, as stated in the MoU, researchers will collaborate on mapping mitochondrial physiology and medicine. The creation of such a unique well-coordinated network of senior researchers and young investigators will definitely put an added value to existing data and publications, will increase quality and validity of the research, will bring new possibilities of data sharing and data mining and will hopefully bring us to the era of more economical research by saving resources through standardised and harmnonised protocols, where the measuremnts will not be wasted due to lack of comparability. - Nebojsa Lalic (2016).
- Our research interest is oriented to reproductive endocrinology; especially to mechanisms that govern steroidogenesis of testicular Leydig cells in disturbed homeostasis. We use several in vivo models:
- stress -/+ systemic or intratesticular blockade of different stress receptors (alpha1ADR; betaADR; GR)
- hypogonadal-hypogonadism -/+ testosterone-replacement therapy
- androgens anabolics treatment -/+ blockade od AR
- aged rats -/+ Viagra-treatment
- biological clock - Silvana Andric (2016).
- I took a look to the MoU and the different working groups. You know well my research focus and having this into account I am interested in actions related to Working group 1 (Respirometric reference protocols, Interlaboratory proficiency test, Instrumental platforms). Working group 2 and 3: I have generated multiple mitochondrial respiration data sets in different mouse tissues (mainly in skeletal muscle, hypothalamus, liver and white adipose tissue). Currently we are also performing these assays in white adipose tissue from human patients. Hence I am interested in all milestones of WG 2 and 3. WG 5 is related to WG 1 and as you know I have interest in training, dissemination and also together with my collaborators (if needed) we could help with data sets and a data interpretation. Overall, I have a general interest on this Action and my active role in MITOEAGLE could be discussed. I could be of help participating in some of the tasks where my expertise and interest fit best. We will be forming and interdisciplinary research group with four group leaders: JC Perales, R Guimera, M Sales-Pardo and PM Garcia-Roves with two different affiliations, University of Barcelona (JCP and PMG-R - Energy metabolism) and University Rovira I Virgili (RG and MS – Data base, statistics, data mining). - Pablo M Garcia-Roves (2016).
- I will be pleased to contribute to the tasks of WG1 by developing interlaboratory proficiency tests and instrumental platform comparison. Moreover, as a (potential) MC member of MitoEAGLE I can also offer visibility to the Action itself (i.e. WG5: MITOEAGLE dissemination and training, according to mitoglobal.org webpages) by establishing new interactions with the International Coenzyme Q10 Association (ICQA) where I act as a member of the Executive Committee. ICQA organizes biennal conferences on new highlights of Coenzyme Q research. In 2015, we held the conference in Bologna (http://conference2015.wix.com/icqaconference) and we included in the program also some scientific sessions dedicated to mitochondrial bioenergetics and diseases. I will be pleased to enforce cooperation between ICQA and MitoEAGLE. - Maria Luisa Genova (2016).
- We found the scientific content and objectives of the call very interesting and compatible with our wide expertise on oxysterol analysis in food and biological systems. Our group has a large experience on lipid composition and their degradation products (volatile and non-volatile), as well as on sensory analysis. Moreover, we are currently involved in the H2020-GERI-2014-1 project "PLOTINA" ("Promoting gender balance and inclusion in research, innovation and training") with Prof. Gallina Toschi (in cc) as coordinator; the project actually includes gendered case studies where gender and sex variables are considered. You can find further information about our expertises, publications and the PLOTINA project at the following webpages: https://www.unibo.it/sitoweb/maria.rodriguez/, https://www.unibo.it/sitoweb/tullia.gallinatoschi/, http://www.unibo.it/en/research/projects-and-initiatives/research-projects-horizon-2020-1/plotina - Maria Teresa Rodriguez Estrada (2016).
- As you know, our current/developing interests are with "evolution" (species comparisons) and "environment" (hypoxia and cold exposure), but have also experience and interest in assessing effects of "lifestyle" (diet, exercise) and "aging" as well (in rodents). - Adam J Chicco (2016).
- Congratulations on the considerable progress with MITOEAGLE! I am keen to participate in this exciting project and I’m sure I can make a valuable contribution to it's success. I have extensive experience performing respirometry assays in human muscle biopsies, myoblasts/tubes and mouse tissues in the contexts of aging, obesity, exercise and inactivity. I feel that I could contribute to any of the five working groups, but particularly to WG2, WG4, and WG5. I am also happy and willing to serve on the Management Committee if needed. Again, congratulations on organizing such an exciting project. - Paul M Coen (2016).
Projects in progress
- Interlaboratory proficiency ring test
- Testing of software components
- Concepts and terminology on mitochondrial physiology
- Procedures and experimental protocols for the evaluation of mitochondrial capacities, library of protocols
- Data management system (DMS)
- Database
Next steps
- Best practices for implementation of analytical and process standards.
References
- General requirements for the competence of testing and calibration laboratories » ISO/IEC 17025:2005