Talk:MitoEAGLE protocols, terminology, documentation

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Participants

CountryCityContact
ArmeniaYerevanTrchounian K
AustraliaClaytonWolff Jonci Nikolai
AustraliaMelbourne, VICMuccini Anna Maria
AustraliaNedlandsFilipovska Aleksandra
AustraliaQueenslandFisher Joshua J
AustraliaNedlandsPerks Kara L
AustraliaSyndeyTon Riccardo
AustraliaVictoriaWinwood-Smith Hugh
AustraliaMelbourneHewakapuge Sudinna
AustraliaBurwoodTrewin Adam J
AustraliaMelbourneSaner Nicholas
AustraliaVictoriaVan Bergen Nicole J
AustraliaMelbourneLionett S
AustraliaClayton VictoriaAlton Lesley
AustraliaMelbourneCassar Samantha
AustraliaMelbourneStepto Nigel K
AustraliaNewtown, SydneyStocker Roland
AustraliaGeelong, VictoriaMcKenzie Matthew
AustraliaParkvilleHardee Justin P
AustraliaSydneyKrycer James R
AustraliaSydneyHoehn Kyle L
AustraliaQueenslandDong Lan-Feng
AustraliaMelbourneCoughlan Melinda T
AustraliaMelbourneGenders Amanda J
AustraliaMelbourneFerri Alessandra
AustraliaMelobourneBotella Javier
AustraliaQueenslandHolland Olivia J
AustraliaSouthportVidimce Josif
AustraliaMelbourneKuang Jujiao
AustraliaMelbourneWhitfield Jamie
AustraliaQueenslandRadenkovic Filip
AustriaInnsbruckDoerrier Carolina
AustriaInnsbruckMeszaros Andras
AustriaInnsbruckBurtscher Martin
AustriaInnsbruckBergmeister Lisa
AustriaInnsbruckAntunes Diana
AustriaInnsbruckThapa Maheshwor
AustriaInnsbruckKapferer Werner
AustriaInnsbruckSchartner Melanie
AustriaInnsbruckGallee Leon
AustriaInnsbruckCardoso Luiza HD
AustriaInnsbruckOrynbayeva Zulfiya
AustriaInnsbruckGarcia-Souza Luiz F
AustriaInnsbruckDi Marcello Marco
AustriaInnsbruckLaner Verena
AustriaInnsbruckCecatto Cristiane
AustriaInnsbruckGoebel G
AustriaInnsbruckGnaiger Erich
AustriaInnsbruckPerez Valencia Juan Alberto
AustriaInnsbruckHaider Markus
AustriaInnsbruckSchwarzer Christoph
AustriaInnsbruckPassrugger Manuela
AustriaInnsbruckKlocker Helmut
AustriaKlosterneuburgSazanov Leonid A
AustriaInnsbruckKarabatsiakis Alexander
AustriaInnsbruckHuete-Ortega Maria
AustriaInnsbruckKeller Markus A
AustriaViennaKozlov Andrey V
AustriaInnsbruckSchmitt Sabine
AustriaSalzburgKofler B
AustriaInnsbruckPlangger Mario
AustriaInnsbruckNindl Veronika
AustriaInnsbruckKomlodi Timea
AustriaInnsbruckHoppel Florian
AustriaInnsbruckOroboros
AustriaInnsbruckIglesias-Gonzalez Javier
BelgiumNamurArnould Thierry
BelgiumLeuven, FlandersBaekelandt V
BelgiumLiegeVotion Dominique-Marie
BelgiumBrusselsSonveaux P
BelguimSart Tilman (Liège)Kruse C
BrasilPorto AlegreHaas Clarissa
BrasiliaCampinasRopelle Eduardo R
BrazilRio de JaneiroGalina Antonio
BrazilSanta Maria - RSGoncalves Debora Farina
BrazilRio de JaneiroOliveira Marcus F
BrazilSao PauloSilber Ariel Mariano
BrazilSanta Maria - Rio Grande do SulSoares Felix Alexandre Antunes
BrazilRio de JaneiroVieyra Adalberto
BrazilCampinasVercesi Anibal E
BrazilSão PauloKowaltowski Alicia J
BrazilSão PauloCrispim Marcell
BrazilJaboticabalOliveira Marcos Tulio
CanadaMoncton, NBPichaud Nicolas
CanadaMontréalBreton Sophie
CanadaEdmonton (Alberta)Lemieux Helene
CanadaCharlottetownAdiele Reginald C
CanadaMontreal, QuebecBergdahl Andreas
CanadaEdmontonHan Woo Hyun
CanadaRimouskiMunro Daniel
CanadaQuebecJoseph Vincent
CanadaEdmontonZaugg Michael
CanadaSaint JohnPulinilkunnil Thomas
CanadaRimouskiBlier Pierre U
CanadaVancouverBovard Josh
CanadaHamiltonTarnopolsky Mark A
CanadaEdmontonLucchinetti Eliana
CanadaMontrealBettinazzi Stefano
CanadaWinnipegChowdhury Subir Kumar
CanadaAntigonishWeir M
CanadaOttawaDarveau Charles-A
CanadaOttawaHarper Mary-Ellen
CanadaGuelphHolloway Graham P
CanadaHamiltonScott Graham R
CanadaVancouver, BCTausan Daniel
ChileCoyhaiqueJana Prado Fabian
ChinaXi`anLiu Jiankang
ChinaPukou, Nanjing,Gan Zhenji
ChinaFuzhou, Fujian ProvinceChen Qi
ChinaTianjinZhang Yong
CroatiaSplitLjubkovic M
CroatiaZagrebSaric A
CroatiaZagrebCurik I
CroatiaZagrebRubelj Ivica
Czech RepublicVestecRohlena Jakub
Czech RepublicVestecEzrova Zuzana
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Czech RepublicHradec KraloveSobotka Ondrej
Czech RepublicVestecDavidova Eliska
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Czech RepublicPlzen -Severni predmestiJedlicka Jan
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DenmarkCopenhagen NMortensen OH
DenmarkRoskildeDalgaard Louise T
DenmarkHvidovreNehlin Jan O
DenmarkCopenhagenDela Flemming
DenmarkCopenhagen NMaise Chroeis K
DenmarkCopenhagenNielsen B
DenmarkCopenhagenLarsen Steen
DenmarkCopenhagenKraunsoee R
DenmarkCopenhagenSoendergaard SD
DenmarkCopenhagenGonzalez-Franquesa Alba
DenmarkAarhus NBoetker Hans Erik
DenmarkAarhus NJespersen Nichlas Riise
EgyptCairoAli Sameh S
EgyptCairoAbdel-Rahman Engy Ali
EstoniaTallinnPuurand Marju
EstoniaTallinnKarro N
EstoniaTallinnLaasmaa Martin
EstoniaTallinnKaambre Tuuli
EstoniaTallinnKlepinin Aleksandr
EstoniaTartuPaju K
EstoniaTallinnShevchuk Igor
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FinlandHelsinkiSuomalainen Wartiovaara Anu
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GermanyNeuherbergEiner C
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JapanSapporoYokota Takashi
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JapanSapporoMatsumoto J
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LatviaRigaJansone Baiba
LatviaRigaLiepins Edgars
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LatviaRigaDambrova Maija
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MalaysiaKuala LumpurHassan Hazirah
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MexicoTlalpanRodriguez-Enriquez Sara
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MexicoMexico D.F.Moreno-Sanchez Rafael
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NetherlandsMaastrichtGrevendonk L
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PolandLodzLabieniec-Watala Magdalena
PolandWarsawSzewczyk Adam
PolandWarsawWasilewski M
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SwitzerlandLausanneBurtscher Johannes
SwitzerlandLausanneAmati Francesca
SwitzerlandLausanneZalachoras I
TaiwanChanghua CityWei Yau-Huei
The NetherlandsMaastrichtNabben Miranda
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The NetherlandsBT AmsterdamWuest Rob CI
The NetherlandsGroningenBakker Barbara M
The NetherlandsMaastrichtSchrauwen Patrick
UKCambridgeBrown Guy C
UKLondonLane Nick
UKCanterbury, KentGourlay Campbell W
UKLondonRodriguez Enrique
USAFairbanksCoker Robert H
USANashvilleWasserman David H
USALouisvilleSkolik Robert A
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USABaltimoreGonzalez-Freire Marta
USABlacksburgBrown David A
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USAKansas CityThyfault John P
USASt. LouisAbumrad Nada A
USAWakefieldVarricchio Frederick
USAEast LansingZhang Yizhu
USALa JollaSchilling Jan M
USABethesdaFessel Joshua Patrick
USAPittsburghJurczak Michael J
USAAuroraMoreau Kerrie
USASalt Lake CityChaurasia Bhagirath
USAPhiladelphiaTomar Dhanendra
USABostonKristal Bruce S
USAClevelandHoppel Charles L
USASan DiegoPatel Hemal H
USAAnn ArborTyrrell Daniel J
USAFort CollinsLi Puma Lance C
USASt. LouisPietka Terri A
USABaltimore, MarylandScheibye-Knudsen Morten
USAIndianapolisBrozinick Joseph T
USAEast LansingBazil Jason N
USAOmahaKim Julian KS
USAOrlandoSparks Lauren M
USANew YorkGalkin Alexander
USAMiamiFontanesi F
USAAkronCohen Bruce H
USARochesterNair K Sreekumaran
USAAtlantaJang Young Charles
USAPhiladelphiaGanetzky Rebecca
USALos AngelesKwast Kurt E
USAChicagoDayanidhi Sudarshan
USAGainesvilleLeeuwenburgh Christiaan
USAIowa CityWagner Brett A
USANorfolkLai Nicola
USAOklahoma CityAhn Bumsoo
USACorvallisBatterson Philip M
USADavisAdams Sean H
USAAuroraFord Ellen
USACorvallisNewsom Sean A
USAProvoHancock Chad R
USAFort CollinsChicco Adam J
USADentonDzialowski Edward M
USAPhiladelphiaPiel Sarah
USATorranceRossiter Harry B
USAGalvestonDurham William J
USAStillwaterDavis Michael S
USABirminghamMoellering Douglas R
USADenverVan Hove Johan
USAJupiterTrivigno Catherine
USARochesterLanza Ian R
USAPortlandGarlid Keith D
USASan Diego; La JollaMolina Anthony JA
USAMilwaukeeDash Ranjan K
USAKansas CitySwerdlow Russell H
USAOrlandoCoen Paul M
USAFayettevilleIyer Shilpa
USAPhiladelphiaJang David H
USASeattleMarcinek DJ
USABethesdaSharma Pushpa
USAColumbusSims Carrie A
USAPhiladelphiaTowheed Atif
USASan DiegoCannon Daniel T
USALittle RockMacMillan-Crow Lee Ann
USAOrlandoPino Maria F
USABaton RougeIrving Brian A
USAColorado SpringsJacobs RA
USAPuskarich Michael A
USAStanfordStary Creed
USAClevelandTandler Bernard
USABaton RougeHand Steven C
USABerkeleyWilliams Caroline M
USACorvallisRobinson Matthew M
USAOrlandoGoodpaster Bret H
... further results


Comments and discussion

  1. We are mostly interested in two WG: (1) Since we are working mostly with heart and muscles, we are interested in WG 2 - MITOEAGLE data repository in muscle and other tissues. (2) WG 1 - Standard operating procedures and user requirement document: Protocols, terminology, documentation. Since we have an expertise in fatty acid metabolism, we are specially interested in experimental protocols for the evaluation of mitochondrial capacities regarding fatty acid metabolism. Of course our parts of the WG are also in our interest. - Marina Makrecka-Kuka, Edgars Liepinsh (2016).
  2. My role in Mito-EAGLE would be mainly in WG 2 and 4, with a focus on skeletal and cardiac muscle and cultured cardiomyocytes and endothelial cells. I would particularly like to bridge the gap between the group leaders and young investigators, trying to represent the young people who are the active users of the OROBOROS equipment. This will fit in nicely with the goals in WG1. ... Major congratulations on obtaining this beautiful collaborative grant! Very glad to see this network getting the recognition it deserves! Looking forward to being part of this! - Rob C Wuest (2016).
  3. Please, find enclosed my declaration to participate with my team in the following workgroups: WG1, WG2, WG3, WG4. We are now involved and have been in not a distant past – in studying of the following cell /tissue types:
    1. Blood cells, with particular emphasis on platelets and lymphocytes – isolated from animals and humans.
    2. Endothelial cells, fibroblasts, neurocytes (neuroblastoma), astrocytes, cancer cell lines – predominantly cultured.
    3. Hepatocytes, cardiomyocytes, cell isolated from brain – isolated from lab animals. - Cezary Watala (2016).
  4. I have already contacted the portuguese CNC to nominate me for the MC of theaction. Concerning my active participation, taking into account the type of work we do in my lab I could be included in all WGs. As you know we work with mitochondria from liver, muscle and fat, as well as cultured cells from different origins (WG2-4). The elaboration of SOPs (WG1) we can certainly contribute (see the book I edited on Mitochondrial Bioenergetics and in which you contribute a chapter). I believe that everybody can be part of the WG5, but as the coordinators of the action, you and Sandra should decide what is best for the success of the action. - Carlos Palmeira (2016).
  5. I would propose my participation in the WG1: Standard operating procedures and user requirement document: Protocols, terminology, documentation, considering that the goal of WG1 is to establish an integrated and analytic environment linking mitochondrial respiration with external data (cell physiology, anthropometry, spiroergometry, lifestyle, genomics) in the context of physical fitness evaluation and clinical diagnostics. I could give my active contribution in the phase of making platform that will aggregate the Action’s complete knowledge and provides the ability to make diagnostic profiling associated with diseases in particular tasks:
    1. Develop reccomendations for quality control, data reporting and data sharing beyond the published record. Develop concepts on Open Access and institutionalised service for data management, data mining and health-care conforming standards of data interpretation
    2. Provide a summary on strategic dissemination and an education programme for MITOEAGLE.
    3. Considering that we are in process of buying the oxygraph, thus broading capacities of my lab, I would be happy to open the door of my new lab for the innovative inter-laboratory ring test experiments and together with my team contribute in that phase of the MITOEAGLE Action. I look forward to the establishment of MitoGlobal EAGLE network where, as stated in the MoU, researchers will collaborate on mapping mitochondrial physiology and medicine. The creation of such a unique well-coordinated network of senior researchers and young investigators will definitely put an added value to existing data and publications, will increase quality and validity of the research, will bring new possibilities of data sharing and data mining and will hopefully bring us to the era of more economical research by saving resources through standardised and harmnonised protocols, where the measuremnts will not be wasted due to lack of comparability. - Nebojsa Lalic (2016).
  6. Our research interest is oriented to reproductive endocrinology; especially to mechanisms that govern steroidogenesis of testicular Leydig cells in disturbed homeostasis. We use several in vivo models:
    1. stress -/+ systemic or intratesticular blockade of different stress receptors (alpha1ADR; betaADR; GR)
    2. hypogonadal-hypogonadism -/+ testosterone-replacement therapy
    3. androgens anabolics treatment -/+ blockade od AR
    4. aged rats -/+ Viagra-treatment
    5. biological clock - Silvana Andric (2016).
  7. I took a look to the MoU and the different working groups. You know well my research focus and having this into account I am interested in actions related to Working group 1 (Respirometric reference protocols, Interlaboratory proficiency test, Instrumental platforms). Working group 2 and 3: I have generated multiple mitochondrial respiration data sets in different mouse tissues (mainly in skeletal muscle, hypothalamus, liver and white adipose tissue). Currently we are also performing these assays in white adipose tissue from human patients. Hence I am interested in all milestones of WG 2 and 3. WG 5 is related to WG 1 and as you know I have interest in training, dissemination and also together with my collaborators (if needed) we could help with data sets and a data interpretation. Overall, I have a general interest on this Action and my active role in MITOEAGLE could be discussed. I could be of help participating in some of the tasks where my expertise and interest fit best. We will be forming and interdisciplinary research group with four group leaders: JC Perales, R Guimera, M Sales-Pardo and PM Garcia-Roves with two different affiliations, University of Barcelona (JCP and PMG-R - Energy metabolism) and University Rovira I Virgili (RG and MS – Data base, statistics, data mining). - Pablo M Garcia-Roves (2016).
  8. I will be pleased to contribute to the tasks of WG1 by developing interlaboratory proficiency tests and instrumental platform comparison. Moreover, as a (potential) MC member of MitoEAGLE I can also offer visibility to the Action itself (i.e. WG5: MITOEAGLE dissemination and training, according to mitoglobal.org webpages) by establishing new interactions with the International Coenzyme Q10 Association (ICQA) where I act as a member of the Executive Committee. ICQA organizes biennal conferences on new highlights of Coenzyme Q research. In 2015, we held the conference in Bologna (http://conference2015.wix.com/icqaconference) and we included in the program also some scientific sessions dedicated to mitochondrial bioenergetics and diseases. I will be pleased to enforce cooperation between ICQA and MitoEAGLE. - Maria Luisa Genova (2016).
  9. We found the scientific content and objectives of the call very interesting and compatible with our wide expertise on oxysterol analysis in food and biological systems. Our group has a large experience on lipid composition and their degradation products (volatile and non-volatile), as well as on sensory analysis. Moreover, we are currently involved in the H2020-GERI-2014-1 project "PLOTINA" ("Promoting gender balance and inclusion in research, innovation and training") with Prof. Gallina Toschi (in cc) as coordinator; the project actually includes gendered case studies where gender and sex variables are considered. You can find further information about our expertises, publications and the PLOTINA project at the following webpages: https://www.unibo.it/sitoweb/maria.rodriguez/, https://www.unibo.it/sitoweb/tullia.gallinatoschi/, http://www.unibo.it/en/research/projects-and-initiatives/research-projects-horizon-2020-1/plotina - Maria Teresa Rodriguez Estrada (2016).
  10. As you know, our current/developing interests are with "evolution" (species comparisons) and "environment" (hypoxia and cold exposure), but have also experience and interest in assessing effects of "lifestyle" (diet, exercise) and "aging" as well (in rodents). - Adam J Chicco (2016).
  11. Congratulations on the considerable progress with MITOEAGLE! I am keen to participate in this exciting project and I’m sure I can make a valuable contribution to it's success. I have extensive experience performing respirometry assays in human muscle biopsies, myoblasts/tubes and mouse tissues in the contexts of aging, obesity, exercise and inactivity. I feel that I could contribute to any of the five working groups, but particularly to WG2, WG4, and WG5. I am also happy and willing to serve on the Management Committee if needed. Again, congratulations on organizing such an exciting project. - Paul M Coen (2016).
  12. I would be interested to be involved with WG1 and WG2. In particular, I am interested in contributing to developing protocols for multicenter quality control among labs, and for permeabilized muscle bundle respirometry. - Harry B Rossiter (2016).
  13. I think that we could contribute via participation in the evaluation of the protocols, and helping to develop them further. In addition, we have been working on the establishing a database that would allow us to keep protocol details, cell isolation and several other aspects. There is nothing very advanced, but we could contribute to establishing such data collection practice. In addition, we, as opposed to many others, happen to use Strathkelvin respirometer. This alternative hardware would allow us to verify whether the protocols work on them as well. - Marko Vendelin (2016).


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