Template:Keywords: Coupling control

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  The Blue Book 2020
  

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  OXPHOS-, ROUTINE-, ET-, and LEAK states; respiratory capacities (P, R, E, L) corrected for residual oxygen consumption Rox.

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  4-compartmental OXPHOS model. (1) ET capacity E of the noncoupled electron transfer system ETS. OXPHOS capacity P is partitioned into (2) the dissipative LEAK component L, and (3) ADP-stimulated P-L net OXPHOS capacity. (4) If P-L is kinetically limited by a low capacity of the phosphorylation system to utilize the protonmotive force pmF, then the apparent E-P excess capacity is available to drive coupled processes other than phosphorylation P» (ADP to ATP) without competing with P».

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  (P-L)/P is the OXPHOS P-L control efficiency. The biochemical coupling efficiency is independent of kinetic control by the phosphorylation system when expressed as the E-L coupling efficiency, (E-L)/E. (E-P)/E is the kinetic E-P control efficiency.

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Mitochondrial and cellular respiratory rates in coupling control states

OXPHOS-capacity P = P´-Rox

ROUTINE-respiration R = R´-Rox

ET-capacity E = E´-Rox

» Noncoupled respiration - Uncoupler

LEAK-respiration L = L´-Rox

» LEAK-state with ATP

» LEAK-state with oligomycin

» LEAK-state without adenylates

Residual oxygen consumption Rox

• Chance and Williams nomenclature: respiratory states

» State 1

» State 2

» State 3

» State 4

» State 5

Flux control ratios related to coupling in mt-preparations and living cells

» Flux control ratio

» Coupling-control ratio

L/P coupling control ratio, L/P

» Respiratory acceptor control ratio, RCR = P/L

L/R coupling control ratio, L/R

L/E coupling control ratio, L/E

» Uncoupling-control ratio, UCR = E/L

P/E control ratio, P/E

R/E control ratio, R/E

net P/E control ratio, (P-L)/E

net R/E control ratio, (R-L)/E

Net, excess, and reserve capacities of respiration

Net OXPHOS-capacity P-L, P-L

Net ROUTINE-activity R-L, R-L

Net ET-capacity E-L, E-L

ET-excess capacity E-P, E-P

ET-reserve capacity E-R, E-R

Flux control efficiencies related to coupling control ratios

» Flux control efficiency j_Z-Y

OXPHOS-coupling efficiency P-L, j_P-L = (P-L)/P = 1-L/P

ROUTINE-coupling efficiency R-L, j_R-L = (R-L)/R = 1-L/R

ET-coupling efficiency E-L, j_E-L = (E-L)/E = 1-L/E

» Biochemical coupling efficiency

ET-excess control efficiency E-P, j_E-P = (E-P)/E = 1-P/E

ET-reserve control efficiency E-R, j_E-R = (E-R)/E = 1-R/E

General

» Background state

» Basal respiration

» Baseline state

» Coupling-control protocol

» Dyscoupled respiration

» Dyscoupling

» Electron leak

» Electron-transfer-pathway state

» Level flow

» Metabolic control variable

» Oxidative phosphorylation

» Oxygen flow

» Oxygen flux

» Permeabilized cells

» Phosphorylation system

» Proton leak

» Proton slip

» Reference state

» Respiratory state

» Static head

» Uncoupling