Difference between revisions of "MitoFit Science Camp 2016 Kuehtai AT"
From Bioblast
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::::'''1A''': Diagnostic SUIT protocols of mitochondrial fitness. | ::::'''1A''': Diagnostic SUIT protocols of mitochondrial fitness. | ||
::::'''1B''': Quality | ::::'''1B''': Quality management system for mitochondrial respirometry: the MitoFit proficiency test. | ||
::::'''2A''': Mitochondrial assays in blood cells. | ::::'''2A''': Mitochondrial assays in blood cells. | ||
| Line 79: | Line 79: | ||
::: '''MITOEAGLE theme''' | ::: '''MITOEAGLE theme''' | ||
:::: MITOEAGLE aims at providing the first standardized measures to link mitochondrial and physiome performance (heart rate, BMI, blood pressure, blood chemistry, ''V''<sub>O2max</sub>) to understand the myriad of factors that play a role in mitochondrial physiology. The ambitious objective to optimally harmonize protocols across research groups cannot be achieved through projects with limited numbers of partners. Therefore, the COST framework represents an ideal instrument which allows bringing together many groups and experts in the diverse field of mitochondrial physiology. The aim of this Action is to form a unique well-coordinated network of senior researchers and young investigators, to include well established stakeholders, and to establish a spirit of mentorship and collaboration in contrast to fierce competition which characterized early decades of bioenergetics. | :::: [[MITOEAGLE]] aims at providing the first standardized measures to link mitochondrial and physiome performance (heart rate, BMI, blood pressure, blood chemistry, ''V''<sub>O2max</sub>) to understand the myriad of factors that play a role in mitochondrial physiology. The ambitious objective to optimally harmonize protocols across research groups cannot be achieved through projects with limited numbers of partners. Therefore, the COST framework represents an ideal instrument which allows bringing together many groups and experts in the diverse field of mitochondrial physiology. The aim of this Action is to form a unique well-coordinated network of senior researchers and young investigators, to include well established stakeholders, and to establish a spirit of mentorship and collaboration in contrast to fierce competition which characterized early decades of bioenergetics. The COST Action MITOEAGLE aims at developing a quality management system (QMS), defining common standards for measurements in the field of mitochondrial function. | ||
::: | |||
::: '''Increase the value and reduce the noise''' | |||
:::: Enthusiastic and authentic opinion leaders can bring about a change to increase the value and reduce the noise in mitochondrial research specifically, biomedicine at large, and in our society in general. Millions of Euros are wasted on research that is lacking coherent standards. Prespecified and time-stamped protocols are needed, and researchers need to be introduced into adhering to publicly deposited protocols in practice (Begley, Ioannidis 2015). MITOEAGLE recommendations will provide practical guidelines for students, scientists and stakeholders. | |||
=== 1A: Diagnostic SUIT protocols of mitochondrial fitness === | === 1A: Diagnostic SUIT protocols of mitochondrial fitness === | ||
=== 1B: Quality | |||
=== 1B: Quality management system for mitochondrial respirometry: the MitoFit proficiency test. === | |||
:::: The quality of any database is not better than the quality of the primary constituent datasets. Rigorous standards have to be applied to forge information into knowledge. Comparability of data generated by different labs on OXPHOS performance is restricted due to different laboratory protocols applied. Harmonization is clearly required along these lines, including the establishment of mitochondrial reference samples for inter-laboratory proficiency tests, and guidelines for data analysis and reporting (http://www.equator-network.org/). | :::: The quality of any database is not better than the quality of the primary constituent datasets. Rigorous standards have to be applied to forge information into knowledge. Comparability of data generated by different labs on OXPHOS performance is restricted due to different laboratory protocols applied. Harmonization is clearly required along these lines, including the establishment of mitochondrial reference samples for inter-laboratory proficiency tests, and guidelines for data analysis and reporting (http://www.equator-network.org/). | ||
:::: There is no QMS commonly available in the field of mitochondrial respirometry and bioenergetics. Without networking on a large scale it is not possible to implement a widely or even generally accepted set of standards that is required for harmonization of datasets. | |||
:::: MITOEAGLE will elaborate definitions of fundamental terms as required by the data management system, including a review on normalization of the data (tissue mass, cell protein, mt-markers such as mt-volume, mt-protein, mt-marker enzymes, flux ratios). | |||
=== 2A: Mitochondrial assays in blood cells === | === 2A: Mitochondrial assays in blood cells === | ||
=== 2B: Cell culture models versus tissue samples: - Brain and neuronal cells - Adipose tissue - Other === | === 2B: Cell culture models versus tissue samples: - Brain and neuronal cells - Adipose tissue - Other === | ||
| Line 95: | Line 103: | ||
=== 4A: Towards a database on mt-respiratory physiology: from laboratory standards to inter-laboratory harmonization === | === 4A: Towards a database on mt-respiratory physiology: from laboratory standards to inter-laboratory harmonization === | ||
:::: Adherence to common standards is likely to increase the proportion of true findings (Ioannidis 2005; Fortier et al 2010). A data bank with harmonized datasets will advance significantly the field of human and comparative bioenergetics research and provide the level of knowledge required for helping health systems to evolve. | |||
:::: The complexity of evolutionary background, age, gender, lifestyle and environment (EAGLE) linked to mitochondrial function cannot be addressed in the conventional small-scale studies with 20 subjects (rather than 2,000 or 200,000). To come anywhere near to large sample sizes, pooling of information between studies is essential on a global scale. | |||
:::: Scientists may refute the challenge of comparing, optimizing and even pre-registering their protocols, when employment and assessment procedures exert stronger pressures on unhealthy competition by high-profile publication than on time-consuming quality control by collaboration. Young investigators may lack a broad perspective on methodological standards and may be reluctant to deviate from a trotten path. MITOEAGLE, however, will bring a large group of mitochondrial experts together by demonstrating the added value for each individual project, when harmonization of protocols will allow scattered data to be combined as the only means to overcome severe limitations of the traditional scientific approach (Fortier et al 2010). | |||
:::: Rigorous standardization is the gold standard for data pooling, but may not be achievable at the present level of research in mt-physiology, where different preparation protocols, incubation media and respiratory protocols are used. Therefore, unrealistic efforts towards developing and implementing a defined standard will be replaced by a translation strategy: Major subprotocols (e.g. different respiration media) will be compared (facilitated by STSM and workshops) to allow quantitative conversion of results obtained by different groups following their specific ‘standard’ operating procedures. This will be a first evolutionary step towards harmonization and standardization. | |||
=== 4B: Mitochondrial health: from molecules, cells and tissues - to the body and mind === | === 4B: Mitochondrial health: from molecules, cells and tissues - to the body and mind === | ||
:::: Diseases that are strongly related to a sedentary life style are spreading world-wide at an epidemic scale. Mitochondrial dysfunction is increasingly associated with the progression of such pathologies: cause or consequence? There is currently no regimented, quantitative system, or database organised to routinely test, compare and monitor mitochondrial capacities within individuals, populations, or among populations. This reflects the need for scientific innovation and represents a shortcoming in the health system of our modern, rapidly ageing society. Traditional lifestyles of ‘remote’ human populations are dwindling such that these highly significant windows into our evolutionary past are about to becoming closed, forever. While studies of mitochondrial DNA (mtDNA) have become increasingly established in the search for the evolutionary history and diversification of the human species, mitochondrial function remains comparatively unexplored, but may turn out to provide key information for preventing diseases which become prevalent upon transition to a Westernized lifestyle. The presently observed acceleration of research on cell and mitochondrial respiration represents a challenge to transform scientific information into knowledge and translate complex results on mitochondrial (dys)function into a patient-related mitochondrial score. Diagnostic standards, however, are lacking. A concerted action of the scientific community is required to implement quality control systems and optimally harmonize protocols across research groups. The primary challenge is to ‘increase value and reduce waste in research design, conduct, and analysis’, to separate the signal from the noise (Ioannidis 2005; The Lanzet 2014): ‘The signal is the truth. The noise is what distracts us from the truth’ ([[Silver 2012 Penguin Press |Silver N: The signal and the noise. The art and science of prediction. Penguin Press 2012]]). | :::: Diseases that are strongly related to a sedentary life style are spreading world-wide at an epidemic scale. Mitochondrial dysfunction is increasingly associated with the progression of such pathologies: cause or consequence? There is currently no regimented, quantitative system, or database organised to routinely test, compare and monitor mitochondrial capacities within individuals, populations, or among populations. This reflects the need for scientific innovation and represents a shortcoming in the health system of our modern, rapidly ageing society. Traditional lifestyles of ‘remote’ human populations are dwindling such that these highly significant windows into our evolutionary past are about to becoming closed, forever. While studies of mitochondrial DNA (mtDNA) have become increasingly established in the search for the evolutionary history and diversification of the human species, mitochondrial function remains comparatively unexplored, but may turn out to provide key information for preventing diseases which become prevalent upon transition to a Westernized lifestyle. The presently observed acceleration of research on cell and mitochondrial respiration represents a challenge to transform scientific information into knowledge and translate complex results on mitochondrial (dys)function into a patient-related mitochondrial score. Diagnostic standards, however, are lacking. A concerted action of the scientific community is required to implement quality control systems and optimally harmonize protocols across research groups. The primary challenge is to ‘increase value and reduce waste in research design, conduct, and analysis’, to separate the signal from the noise (Ioannidis 2005; The Lanzet 2014): ‘The signal is the truth. The noise is what distracts us from the truth’ ([[Silver 2012 Penguin Press |Silver N: The signal and the noise. The art and science of prediction. Penguin Press 2012]]). | ||
Revision as of 14:01, 4 May 2016
MitoFit Science Camp 2016 Kuehtai AT
 Kuehtai AT, 2016 Jul 07-13. 1st MitoFit Training Camp |
» MitoFit
OROBOROS (2016-07-07) MitoGlobal
Abstract: 2016 Jul 07-13, Kuehtai, AT. The project MitoFit aims at developing novel laboratory standards and diagnostic monitoring of a mitochondrial fitness score. With an international team of outstanding mitochondrial experts, the MitoFit Training Camp will provide a unique opportunity to receive first-hand introductions to state-of-the-art diagnostic monitoring of mitochondrial respiratory function. Diverse areas are covered such as protective medicine, exercise physiology, mitochondrial pharmacology, aging, and comparative mitochondrial physiology (cell types, tissues, species). The MitoFit Training Camp will address key topics of the COST Action MITOEAGLE project and provide a key opportunity to prepare the 1st Management Committee Meeting.
• O2k-Network Lab: AT Innsbruck OROBOROS
Labels: MiParea: mt-Awareness
2016, ORO, IOC, O2k-Network, MitoFit, Next
MitoFit Training Camp - for and with mitochondrial scientists
- Date: 2016, July 07-13
- Venue: High Altitude Training Center Kühtai, Kuehtai (Tyrol, Austria), 2020 m a.s.l. (42 min from Innsbruck).
- Maximum 99 participants.
- Immediately following EBEC2016 (July 2-7) organized by Paolo Bernardi in Riva del Garda (2.5 hours from Innsbruck).
Programme structure
- 1A: Diagnostic SUIT protocols of mitochondrial fitness.
- 1B: Quality management system for mitochondrial respirometry: the MitoFit proficiency test.
- 2A: Mitochondrial assays in blood cells.
- 2B: Cell culture models versus tissue samples: - Brain and neuronal cells - Adipose tissue - Other
- 3A: Skeletal and cardiac muscle fibres - towards a data repository.
- 3B: Excursion.
- 4A: Towards a database on mt-respiratory physiology: from laboratory standards to inter-laboratory harmonization.
- 4B: Mitochondrial health: from molecules, cells and tissues - to the body and mind.
- 5A: Open topics. Experimental working groups.
- 5B: Open topics. Experimental working groups.
July 07 Th 08 Fr 09 Sa 10 Su 11 Mo 12 Tu 13 We 07:00-08:00 Arrival Morning Action Morning Action Morning Action Morning Action Morning Action 08:00-09:00 Break(fast) Break(fast) Break(fast) Break(fast) Break(fast) Breakfast 09:00-10:15 Block 1A Block 2A Block 3A Block 4A Block 5A Departure 10:45-12:00 12:00-15:00 Lunch & Action Lunch & Action Lunch & Action Lunch & Action Lunch & Action 15:30-16:45 Block 1B Block 2B Excursion Block 4B Block 5B 17:15-18:30 19:00-20:30 Dinner Dinner Dinner Dinner Dinner Dinner 20:30-21:15 Evening topic Evening topic Keynote MITOEAGLE and MoTrPAC MitoFit party
Preliminary programme
- The programme is designed as a global introduction to the perspectives of the COST Action MITOEAGLE.
- High-resolution respirometry (HRR) will be demonstrated at various levels, integrating an IOC (O2k-Workshop on HRR).
- Blocks are scheduled to merge introductory lectures, panels, and poster flashes.
- MITOEAGLE theme
- MITOEAGLE aims at providing the first standardized measures to link mitochondrial and physiome performance (heart rate, BMI, blood pressure, blood chemistry, VO2max) to understand the myriad of factors that play a role in mitochondrial physiology. The ambitious objective to optimally harmonize protocols across research groups cannot be achieved through projects with limited numbers of partners. Therefore, the COST framework represents an ideal instrument which allows bringing together many groups and experts in the diverse field of mitochondrial physiology. The aim of this Action is to form a unique well-coordinated network of senior researchers and young investigators, to include well established stakeholders, and to establish a spirit of mentorship and collaboration in contrast to fierce competition which characterized early decades of bioenergetics. The COST Action MITOEAGLE aims at developing a quality management system (QMS), defining common standards for measurements in the field of mitochondrial function.
- MITOEAGLE theme
- Increase the value and reduce the noise
- Enthusiastic and authentic opinion leaders can bring about a change to increase the value and reduce the noise in mitochondrial research specifically, biomedicine at large, and in our society in general. Millions of Euros are wasted on research that is lacking coherent standards. Prespecified and time-stamped protocols are needed, and researchers need to be introduced into adhering to publicly deposited protocols in practice (Begley, Ioannidis 2015). MITOEAGLE recommendations will provide practical guidelines for students, scientists and stakeholders.
- Increase the value and reduce the noise
1A: Diagnostic SUIT protocols of mitochondrial fitness
1B: Quality management system for mitochondrial respirometry: the MitoFit proficiency test.
- The quality of any database is not better than the quality of the primary constituent datasets. Rigorous standards have to be applied to forge information into knowledge. Comparability of data generated by different labs on OXPHOS performance is restricted due to different laboratory protocols applied. Harmonization is clearly required along these lines, including the establishment of mitochondrial reference samples for inter-laboratory proficiency tests, and guidelines for data analysis and reporting (http://www.equator-network.org/).
- There is no QMS commonly available in the field of mitochondrial respirometry and bioenergetics. Without networking on a large scale it is not possible to implement a widely or even generally accepted set of standards that is required for harmonization of datasets.
- MITOEAGLE will elaborate definitions of fundamental terms as required by the data management system, including a review on normalization of the data (tissue mass, cell protein, mt-markers such as mt-volume, mt-protein, mt-marker enzymes, flux ratios).
2A: Mitochondrial assays in blood cells
2B: Cell culture models versus tissue samples: - Brain and neuronal cells - Adipose tissue - Other
3A: Skeletal and cardiac muscle fibres - towards a data repository
3B: Excursion
4A: Towards a database on mt-respiratory physiology: from laboratory standards to inter-laboratory harmonization
- Adherence to common standards is likely to increase the proportion of true findings (Ioannidis 2005; Fortier et al 2010). A data bank with harmonized datasets will advance significantly the field of human and comparative bioenergetics research and provide the level of knowledge required for helping health systems to evolve.
- The complexity of evolutionary background, age, gender, lifestyle and environment (EAGLE) linked to mitochondrial function cannot be addressed in the conventional small-scale studies with 20 subjects (rather than 2,000 or 200,000). To come anywhere near to large sample sizes, pooling of information between studies is essential on a global scale.
- Scientists may refute the challenge of comparing, optimizing and even pre-registering their protocols, when employment and assessment procedures exert stronger pressures on unhealthy competition by high-profile publication than on time-consuming quality control by collaboration. Young investigators may lack a broad perspective on methodological standards and may be reluctant to deviate from a trotten path. MITOEAGLE, however, will bring a large group of mitochondrial experts together by demonstrating the added value for each individual project, when harmonization of protocols will allow scattered data to be combined as the only means to overcome severe limitations of the traditional scientific approach (Fortier et al 2010).
- Rigorous standardization is the gold standard for data pooling, but may not be achievable at the present level of research in mt-physiology, where different preparation protocols, incubation media and respiratory protocols are used. Therefore, unrealistic efforts towards developing and implementing a defined standard will be replaced by a translation strategy: Major subprotocols (e.g. different respiration media) will be compared (facilitated by STSM and workshops) to allow quantitative conversion of results obtained by different groups following their specific ‘standard’ operating procedures. This will be a first evolutionary step towards harmonization and standardization.
4B: Mitochondrial health: from molecules, cells and tissues - to the body and mind
- Diseases that are strongly related to a sedentary life style are spreading world-wide at an epidemic scale. Mitochondrial dysfunction is increasingly associated with the progression of such pathologies: cause or consequence? There is currently no regimented, quantitative system, or database organised to routinely test, compare and monitor mitochondrial capacities within individuals, populations, or among populations. This reflects the need for scientific innovation and represents a shortcoming in the health system of our modern, rapidly ageing society. Traditional lifestyles of ‘remote’ human populations are dwindling such that these highly significant windows into our evolutionary past are about to becoming closed, forever. While studies of mitochondrial DNA (mtDNA) have become increasingly established in the search for the evolutionary history and diversification of the human species, mitochondrial function remains comparatively unexplored, but may turn out to provide key information for preventing diseases which become prevalent upon transition to a Westernized lifestyle. The presently observed acceleration of research on cell and mitochondrial respiration represents a challenge to transform scientific information into knowledge and translate complex results on mitochondrial (dys)function into a patient-related mitochondrial score. Diagnostic standards, however, are lacking. A concerted action of the scientific community is required to implement quality control systems and optimally harmonize protocols across research groups. The primary challenge is to ‘increase value and reduce waste in research design, conduct, and analysis’, to separate the signal from the noise (Ioannidis 2005; The Lanzet 2014): ‘The signal is the truth. The noise is what distracts us from the truth’ (Silver N: The signal and the noise. The art and science of prediction. Penguin Press 2012).
4C: MITOEAGLE
- The objective of the MITOEAGLE network is to improve our knowledge on mitochondrial function in health and disease related to Evolution, Age, Gender, Lifestyle and Environment. Every study of mitochondrial (mt) function and disease is faced with EAGLE as the essential background conditions characterizing the individual patient, subject, study group, species, tissue or even cell line. To address the complex interrelationships of EAGLE with an initial focus on humans and rodent models, the network will enhance the value of each individual study by starting to analyse and catalog data beyond the published record. Highlighting the topic of gender and mitochondrial function, unique new information will emerge on human biology from the development of a European reference database. Protocols, technologies and standard procedures will be compared and strategies defined for improvement of quality control. An inter-laboratory ring test will be established as a world-wide innovation in the field of mitochondrial respiratory physiology. The expertise gained and new standards developed will be integrated into a strategic dissemination and education programme for mitochondrial phenotyping, aiming at an expanding European and MitoGlobal EAGLE network where researchers collaborate on mapping mitochondrial physiology and medicine, complementary to established mtDNA databases.
- Every study of mitochondrial function and disease in human tissues and cells is faced with Evolution, Age, Gender, Lifestyle and Environment (EAGLE) as essential background conditions characterizing the individual patient, subject, study group, species, tissue or – to some extent - cell line. This range of factors is too wide to be accommodated in any single project on mitochondrial respiration. Only a large and well coordinated network can manage to generate the necessary number of consistent data to address the complexity of EAGLE. The MITOEAGLE knowledge management network will be a strategic innovation to develop harmonization protocols towards generating a rigorously monitored data repository on mitochondrial respiratory function. A MITOEAGLE data management system is necessary to interrelate results of a large number of studies, to interpret pathological phenotypes, and to set results into the multidimensional context of EAGLE. Clearly, MITOEAGLE must be operated by mitochondrial specialists on a global scale. MITOEAGLE will be a gateway and milestone to better diagnose mitochondrial respiratory defects which are linked to various age-related health risks, including cardiovascular and degenerative diseases, such as type 2 diabetes, neurodegenerative diseases (Alzheimer’s, Parkinson’s, Huntington’s), and several types of cancer (Murdoch 2013 JAMA).
=== 5: Open topics. Experimental working groups.
Topics included
- » Towards a knowledge management platform for mitochondrial physiology - feeding the database.
- » Do bioenergetics and mitochondrial physiology need a consistent nomenclature to become MitoFit?
- » Thermodynamics of core energy metabolism: chemical and electrical fluxes and forces - how textbook errors generate noise instead of information.
- Sign in as a subject: ergometric tests and MitoFit score of blood cells (limited number of participants).
- MitoFit: expertise by experience - high altitude sports and physiotherapy in the Kuehtai environment
» http://www.hoehentraining-kuehtai.at/
» http://www.sporttherapie-huber.at- » Meet the MitoFit athlete: a unique training experience
- » Meet the MitoFit expert: groups and one-on-one sessions
- » Walks&Talks
* IOC - O2k-Workshop.
Registration, travel information and support
- Early bird registration before May 15.
- Scholarships:
- OROBOROS INSTRUMENTS will support students with scholarships of EUR 200.-/person. Up to 25 scholarships will be granted.
- Send us your abstract in order to apply for a scholarship. The abstract will then be evaluated by the MitoFit Scientific Committee.
- Contact: Laner Verena, OROBOROS INSTRUMENTS, MitoFit project manager
Abstracts
- » Abstracts will be accepted until May 15. Please indicate your preferred session number.
- » Abstract format MitoFit
- P-flash = Flash presentations or poster flashes: 5 min plus 2 min discussion.
- Poster = Poster presentation: Posters are up during the entire MitoFit Training Camp, providing an excellent opportunity for person-to-person discussions.
Lecturers
- Preliminary list of confirmed speakers (work in progress)
- Brown David, Greenville, US
- Burtscher Martin, Innsbruck, AT
- Chicco Adam, Fort Collins, US
- Coen Paul, Orlando, US
- Doerrier Carolina, Innsbruck, AT
- Garcia-Roves Pablo Miguel, Barcelona, ES
- Gnaiger Erich, Innsbruck, AT
- Goodpaster Bret, Orlando, US
- Hickey Anthony J, Auckland, NZ
- Hoppel Charles L, Cleveland, US
- Huber Reinhard, Innsbruck, AT
- Kane Daniel, Antigonish, CA
- Krumschnabel Gerhard, Innsbruck, AT
- Lundby Carsten, Zurich, CH
- McManus Meagan, Philadelphia, US
- Molina Anthony JA, Winston-Salem, US
- Neufer P Darrell, Greenville, US
- Ojuka Edward O, Cape Town, ZA
- Sumbalova Zuzana, Bratislava, SK
- Tarnopolsky Mark, Ontario, CA
- Preliminary list of confirmed speakers (work in progress)
Location: High Altitude Training Centre Kuehtai
- Partners
Listed under MitoGlobal Events.
