MiPMap

high-resolution terminology - matching measurements at high-resolution

MiPMap

Description

The project Mitochondrial Physiology Map (MiPMap) is initiated to provide an overview of mitochondrial properties in cell types, tissues and species. As part of MitoPedia, MiPMap may be considered as an information synthase for Comparative Mitochondrial Physiology. Establishing a comprehensive database will require global input and cooperation.

A comparative database of mitochondrial physiology may provide the key for understanding the functional implications of mitochondrial diversity from mouse to man, and evaluation of altered mitochondrial respiratory control patterns in health and disease (Gnaiger 2009).

Abbreviation: MiPMap

Reference: Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle. New perspectives of mitochondrial physiology. Int J Biochem Cell Biol 41: 1837-1845.

MitoPedia methods: Respirometry, Fluorometry, Spectrophotometry 

MitoPedia topics: "Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property. Respiratory state"Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property., Enzyme, Media for respirometry, Inhibitor, Substrate and metabolite, Uncoupler, Permeabilization agent 

MiPMap labels

MiPMap labels provide the backbone of the MiPMap project. The MiPMap labels can be considered as keywords which can be attached to various sources of information (publications). With proper labelling, multidimensional maps of information can be constructed, filtering the sources through various grids.

The MiPMap labels (filters) are grouped into various topics (properties):

1. MiP areas
2. Mammals;models
3. Non-mammals
4. Tissues;cells
5. Cell lines
6. Preparations
7. Enzymes
8. Stress conditions
9. Pathological states
10. Regulation;kinetics
11. Coupling states
12. Substrate states
13. High-resolution respirometry

>> More Information on MiPMap labels

Publications in the MiPMap: mitochondrial properties in organisms (species), cell types and tissues

1. Mammals and model organisms

Work in progress

>>Comparative MiP publications: Species, tissues, cells

As a test case, O2k-Publications are listed according to MiPMap Labels:

>> O2k-Publications:_Topics

For example, in MiPMap you would find information on mouse cell lines and tissues, e.g. mouse kidney mitochondria:

* Is malic enzyme present?

* Is the P/E<1.0 like in mouse brain but different from mouse skeletal muscle?

* Which UCPs are present?

* Preparation of isolated mitochondria, permeabilized tissue, homogenate.

* Which respiration medium is recommended? ->> Do not use media with high K⁺, which inhibits kidney mitochondrial respiration.

>> Is this a general issue for kidney, or is it in addition also a species issue? (according to Calzia E MiR05 works fine with kidney tissue from pig, but not in mouse). >> Limitations of using MiR05 to MiR06Cr

2. Tissues, cell types and cell lines

Or you may find Skeletal muscle mitochondria studied across species:

* Skeletal muscle - Human

->> O2k-Publications: Human_-_Skeletal_muscle

3. Stress conditions and pathological states

Work in progress

Medical and Pharmacological

Initiation

• Presented at Bioblast 2012.
• Focus for MiPconference on Comparative Mitochondrial Physiology.
• Link to Mitochondrial Global Network