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Difference between revisions of "User talk:Tindle Lisa/My sandbox Support page v2"

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<big><big>'''"A reprogramming-based strategy for drug repositioning in patients with mitochondrial DNA-associated Leigh syndrome (MILS)"'''</big></big>
:::::::::::: <big>''Currently Asked Questions - »'''[[O2k-Open Support alert]]'''« </big>  


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__TOC__
== O2k-Open Support resources ==
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'''Startup information'''
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:::: Each O2k-Package includes a remote installation and startup support session with an Oroboros expert, which can be scheduled during any time within the O2k-Warranty period (max. 2 years)
:::::» '''[https://www.oroboros.at/index.php/support-session-request-form/ Register for your session]'''


__TOC__
:::::» [[Installation and startup support session self-study material]]
:::: Upon special request and subject to an additional fee, Oroboros Instruments offers a start-up visit by a scientific application specialist.
:::::» [[O2k on-site training]]
 
:::: Additional reading
:::::» [[MiPNet20.04 O2k-checklist| Getting started with an O2k-experiment]]
:::::» O2k-Workshop self-study material: [https://wiki.oroboros.at/index.php/Virtual_O2k-Workshop_self-study_material#O2k-Basic O2k-Basic]
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'''Quality control'''
 
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[[File:MitoFit-QCS.jpg|right|400px|MitoFit-QCS]]
:::: Many commonly raised issues of O2k-technical support are solved in the newest version of [[DatLab]].     


== "Project Name": "Funding Scheme" ==
:::: The '''O<sub>2</sub> sensor and O2k chamber tests''' are important to eliminate instrumental artefacts (instrumental quality control, QC), distinguished from assay QC addressing problems of [[solution protocols]] (preparation of chemicals), [[sample]] preparation protocols (such as [[mitochondrial preparations]]), and [[SUIT protocols]].
::::*  EJP RD JTC 2020: "PRE-CLINICAL RESEARCH TO DEVELOP EFFECTIVE THERAPIES FOR RARE DISEASES” - Project EJPRD20-010
::::# Clean O2k-chambers and contamination-free incubation medium are required.
::::* '''Duration''': 36 months
::::# Analysis of DatLab files recorded in instrumental QC tests is essential.       
::::* '''Start''': 
::::# Biological experiments are not suitable for trouble shooting.
::::* '''Web''':


== About CureMILS ==
:::: '''1. O<sub>2</sub> sensor test'''
:::: Led by Prof. Dr. Alessandro Prigione of the Heinrich Heine University, the CureMILS project aims to employ a novel approach to enable drug discovery of MILS.
:::: The [[OroboPOS|OroboPOS]] (polarographic oxygen sensor) requires regular service at intervals - these may be more than 1 year. SOPs are available to determine if a sensor service is required at an earlier or later date:
::::» O<sub>2</sub> sensor test: [[Oxygen_sensor_test |'''QC1: Oxygen sensor test''']]     


===Aims===
:::: '''2. O2k-chamber test'''
The project aims to use iPSCs and reprogramming-drived drug discovery for finding therapies against MILS. A personalized medicine approach will be applied and compound screenings on patient-derived neural cells will be carried out. Brain organoids and multi-omics analysis will be used for validations.
:::: If all quality control criteria of the O<sub>2</sub> sensor test are met, the operator can be assured that the quality of the sensor signal is acceptable. Next, the quality of the O2k-chamber assembly has to be tested, described in detail:
1. Establishment of PDE5 inhibitors as reference drugs for MILS
::::» O2k-chamber test: [[Oxygen flux - instrumental background |'''QC2: Instrumental O<sub>2</sub> background test''']]     
2. Large-scale screen of marketed compounds in MILS neural cells
3. Validations of hit compounds in MILS neural cells
4. Validations of hit compounds in neural cells carrying LS-associated Complex I mutations
5. Computational analysis of MILS neural cells and treatments


===Objectives===
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:::: '''WP1''' - Name of person and/or instution responsible for this workpackage
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::::*  Bullet points of WP objectives
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'''Advanced topics'''
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::» O2k-Workshop self-study material: [https://wiki.oroboros.at/index.php/Virtual_O2k-Workshop_self-study_material#O2k-Advanced O2k-Applications: Simultaneous determination of O2 and H2O2 fluxes]


:::: '''WP2''' - Name of person and/or instution responsible for this workpackage
:: You may also find the answers to more advanced topics using the navigation menu above or simply by using the Bioblast search function.:
::::* Bullet points of WP objectives
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| [[Image:O2k-Hardware icon.jpg|50px|link=https://wiki.oroboros.at/index.php/MitoPedia:_O2k_hardware |MitoPedia: O2k hardware]]
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== Coordinator==
| [[Image:O2k-GlossaryFaq icon.jpg|50px|link=https://wiki.oroboros.at/index.php/MitoPedia:_O2k-Open_Support |MitoPedia: O2k-Open Support]]
::::*  [[Prigione Alessandro| Alessandro Prigione, MD, PhD]] - Department of General Pediatrics, Neonatology, and Pediatric Cardiology at Heinrich Heine University, DE - https://www.neurosciences-duesseldorf.de/principal-investigators-and-junior-researchers/alessandro-prigione OR https://www.hhu.de/en/
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::::::# '''MitoPedia: O2k''': find information quickly with MitoPedia, with lists of key words and links to relevant pages.
::::::## [[MitoPedia: O2k hardware]]
::::::## [[MitoPedia: DatLab]]
::::::## [[MitoPedia:_SUIT ]]
::::::# [[O2k-Procedures]]: explain various applications of the O2k with O2k-Demo experiments
::::::# [[O2k-Manual]]: topic specific manuals with step by step help
::::::# [[O2k-Videosupport]]: provides valuable assistance, complementary to the O2k-Manual
::::::# [[MitoPedia: O2k-Open Support|O2k-Glossary]]: full list of key words and links to relevant pages.


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== Network ==
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== Ask an O2k-Expert and the O2k-Open Support agreement ==
===Participants/Beneficiaries===
::::* Fraunhofer IME, Department ScreeningPort,
::::* Institute for Molecular Biology, Innsbruck University


::::* Radboud University Medical Center, Biochemistry
:::: We give you the tools to be self-sufficient, but sometimes you need expert advice or help solving a technical problem. The Oroboros experts will be happy to help in using our O2k-Open Support system. » [https://www.oroboros.at/index.php/o2k-technical-support '''Contact Support''']     
::::* University of Helsinki, Research Program of Stem Cells and Metabolism
::::» [[O2k-Open Support agreement]]
::::» [[O2k repair]]


::::* Institute of Genetics and Animal Biotechnology of the Polish Academy of Sciences, Department of Molecular Biology


::::* Verona University, Department of Diagnostics and Public Health
[[File:O2k-Network.png|left|100px|O2k-Network|link=O2k-Network]]
::::* Luxembourg Centre for Systems Biomedicine (LCSB) University of Luxembourg
== The O2k-Network: An additional resource ==


::::*  International Mito Patients (IMP)
:::: The Oroboros O2k-Network serves to connect and support: Contact for advice on applications of the O2k and high-resolution respirometry - the global network in mitochondrial physiology.     
=== Collaborators ===
::::* Charité University, Clinic for Pediatrics and Neurology


::::* Department of General Pediatrics, Neonatology and Pediatric Cardiology, University Children's Hospital, Heinrich Heine University
::::: '''»''' The O2k-Network Labs: [[O2k-Network|» O2k-Network]]     


::::* Oroboros Instruments GmbH
::::: '''»''' [[O2k-Network_discussion_forum|O2k-Network discussion forum]]     


::::* Foundation IRCCS Neurological Institute C. Besta, Unit of Molecular Neurogenetics
:::: '''»''' [[Oroboros Laboratories|Oroboros O2k-Laboratory]] - open for innovation and cooperation.  
:::: '''»''' [[Oroboros_Science_Scholarship|Oroboros Science Scholarships]] - are offered for focused research projects on mitochondrial physiology and high-resolution respirometry     


::::* University of Milan, Department of Medical Biotechnology and Translational Medicine
[[Category:O2k-Open Support]]


::::* Max Planck Institute for Molecular Genetics
::::* Deutsches MITONET e.V., Friedrich-Baur-Institut




== Oroboros project involvement ==
<br />
:::: Oroboros is a collaborator on the project providing infrastructure and expertise required to conduct respirometric measurements for the project.
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== CureMILS events ==
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{{#ask:[[Category:Publications]] [[Additional label::CureMILS]]
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== CureMILS publications ==


== CureMILS  dissemination ==


==Links==
===Social Media===
===Research Networks===


== References ==
== Additional information ==
== Support ==


This project has received funding from the European Joint Programme on Rare Diseases (EJP RD).


Funded projects: https://www.ejprarediseases.org/index.php/funded-projects-2020/
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Latest revision as of 13:59, 29 July 2021


                  

O2k-Open Support

User talk:Tindle Lisa/My sandbox Support page v2

Currently Asked Questions - »O2k-Open Support alert« 


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