Difference between revisions of "User talk:Tindle Lisa/My sandbox Support page v2"

Revision as of 14:36, 20 May 2021

User talk:Tindle Lisa/My sandbox Support page v2

Currently Asked Questions - »O2k-Open Support alert«

O2k-Quality Control

Many commonly raised issues of O2k-technical support are solved in the newest version of DatLab.

The O₂ sensor and O2k chamber tests are important to eliminate instrumental artefacts (instrumental quality control, QC), distinguished from assay QC addressing problems of solution protocols (preparation of chemicals), sample preparation protocols (such as mitochondrial preparations), and SUIT protocols.

Clean O2k-chambers and contamination-free incubation medium are required.
Analysis of DatLab files recorded in instrumental QC tests is essential.
Biological experiments are not suitable for trouble shooting.

1. O₂ sensor test

The OroboPOS (polarographic oxygen sensor) requires regular service at intervals - these may be more than 1 year. SOPs are available to determine if a sensor service is required at an earlier or later date:

» O₂ sensor test: QC1: Oxygen sensor test

2. O2k-chamber test

If all quality control criteria of the O₂ sensor test are met, the operator can be assured that the quality of the sensor signal is acceptable. Next, the quality of the O2k-chamber assembly has to be tested, described in detail:

» O2k-chamber test: QC2: Instrumental O₂ background test

O2k-Open Support: Installation and startup support session

Each O2k-Package includes a remote installation and startup support session with an Oroboros expert. This online support session will focus on:

Instrumental setup
Quality control
Discussion with our expert

Installation and startup support can be scheduled during any time within the O2k-Warranty period (max. 2 years)

» Register for your session

Upon special request and subject to an additional fee, Oroboros Instruments offers a start-up visit by a scientific application specialist.

» O2k-Start up and Training

O2k-Open Support agreement

The Oroboros experts will be happy to help in using our O2k-Open Support system.

» Search for defective O2k components

» In case you need further technical support and for your valuable feedback, please contact O2k-Tech Support

» O2k-Open Support agreement

» O2k repair

The O2k-Network

The Oroboros O2k-Network serves to connect and support: Contact for advice on applications of the O2k and high-resolution respirometry - the global network in mitochondrial physiology.

» The O2k-Network Labs: » O2k-Network

» O2k-Network discussion forum

» Oroboros O2k-Laboratory - open for innovation and cooperation.

» Oroboros Science Scholarships - are offered for focused research projects on mitochondrial physiology and high-resolution respirometry

@@ Line 1: / Line 1: @@
- {{Template:OROBOROS header page name}}
+{{Template:OROBOROS support page name}}
+{{NextGen-O2k H2020-support}}
+[[File:O2k-support system.jpg|right|350px|Oroboros support system]]
+:::::::::::: <big>''Currently Asked Questions - »'''[[O2k-Open Support alert]]'''« </big>
+__TOC__
-<big><big>'''"A reprogramming-based strategy for drug repositioning in patients with mitochondrial DNA-associated Leigh syndrome (MILS)"'''</big></big>
-[[File:EJP-RD logo.png|right|300px|EJP-RD]]
-__TOC__
+== O2k-Quality Control ==
+[[File:MitoFit-QCS.jpg|right|400px|MitoFit-QCS]]
+:::: Many commonly raised issues of O2k-technical support are solved in the newest version of [[DatLab]].
+:::: The '''O<sub>2</sub> sensor and O2k chamber tests''' are important to eliminate instrumental artefacts (instrumental quality control, QC), distinguished from assay QC addressing problems of [[solution protocols]] (preparation of chemicals), [[sample]] preparation protocols (such as [[mitochondrial preparations]]), and [[SUIT protocols]].
+::::# Clean O2k-chambers and contamination-free incubation medium are required.
+::::# Analysis of DatLab files recorded in instrumental QC tests is essential.
+::::# Biological experiments are not suitable for trouble shooting.
+=== 1. O<sub>2</sub> sensor test ===
+:::: The [[OroboPOS|OroboPOS]] (polarographic oxygen sensor) requires regular service at intervals - these may be more than 1 year. SOPs are available to determine if a sensor service is required at an earlier or later date:
+::::» O<sub>2</sub> sensor test: [[Oxygen_sensor_test |'''QC1: Oxygen sensor test''']]
+=== 2. O2k-chamber test ===
+:::: If all quality control criteria of the O<sub>2</sub> sensor test are met, the operator can be assured that the quality of the sensor signal is acceptable. Next, the quality of the O2k-chamber assembly has to be tested, described in detail:
+::::» O2k-chamber test: [[Oxygen flux - instrumental background |'''QC2: Instrumental O<sub>2</sub> background test''']]
+== O2k-Open Support: Installation and startup support session ==
+:::: Each O2k-Package includes a remote installation and startup support session with an Oroboros expert. This online support session will focus on:
+::::* Instrumental setup
+::::* Quality control
+::::* Discussion with our expert
+:::: Installation and startup support can be scheduled during any time within the O2k-Warranty period (max. 2 years)
+:::::» '''[https://www.oroboros.at/index.php/support-session-request-form/ Register for your session]'''
-== CureMILS: European Joint Programme on Rare Diseases - JTC2020 ==
-::::*  EJP RD JTC 2020: "PRE-CLINICAL RESEARCH TO DEVELOP EFFECTIVE THERAPIES FOR RARE DISEASES” - Project EJPRD20-010
-::::* '''Duration''': 36 months
-::::* '''Start''':
-::::* '''Web''':
-== About CureMILS ==
+:::: Upon special request and subject to an additional fee, Oroboros Instruments offers a start-up visit by a scientific application specialist.
-:::: Mitochondrial DNA (mtDNA)-associated Leigh syndrome (MILS) is a severe early-onset brain disease affecting 1/100,000 newborns. MILS is typically caused by mtDNA mutations in the ATP-generating subunit MT-ATP6. There are no treatments available for MILS. In fact, drug discovery is particularly challenging for MILS. The limited access to patient neural tissue and the difficulty to manipulate mtDNA hinder the development of transgenic animal models and cellular models, which are needed for treatment discovery and development. Led by Prof. Dr. Alessandro Prigione of the Heinrich Heine University, the CureMILS project aims to employ a novel approach to enable drug discovery of MILS.
+:::::» '''[[O2k-Start up and Training]]'''
-===Aims===
-:::: The consortium will employ neural cells generated from MILS patients via cellular reprogramming to carry out a large-scale screening using marketed drugs, thereby allowing the identification of therapeutic strategies.
-:::: The proof-of-concept study demonstrated that this approach is feasible and relevant. The project proposes to extend this approach using a large high-quality library of repurposable compounds (more than 5,500). The consortium will validate hit compounds by combining mitochondrial profiling with multi-omics analysis using various reprogramming-derived neural models (neural progenitors, neurons, brain organoids, and blood-brain barrier cells) from different MILS patients.
+[[File:O2k-Support.jpg|right|200px|O2k-Open Support]]
-===Objectives===
+== O2k-Open Support agreement ==
-:::: The consortium will identify drugs suited for repositioning as interventions in MILS, laying the foundation for a multi-national clinical trial and a concrete path towards a cure for MILS.
-:::: Moreover, we will establish a paradigmatic working pipeline for reprogramming-driven drug discovery and repositioning for rare neurological disorders.
+:::: The Oroboros experts will be happy to help in using our O2k-Open Support system.
+::::» [[Search for defective O2k components]]
+:::: '''»''' In case you need further technical support and for your valuable feedback, please contact [https://www.oroboros.at/index.php/o2k-technical-support '''O2k-Tech Support''']
+::::» [[O2k-Open Support agreement]]
+::::» [[O2k repair]]
-== Coordinator==
-::::*  [[Prigione Alessandro| Prof. Dr. Alessandro Prigione]] - [https://www.uniklinik-duesseldorf.de/en/department-of-general-paediatrics Department of General Pediatrics, Neonatology and Pediatric Cardiology, University Children's Hospital, Heinrich Heine University], DE
+[[File:O2k-Network.png|left|100px|O2k-Network|link=O2k-Network]]
-[[Image:Hhu logo.png‎‎|110px|Heinrich Heine University]]
+== The O2k-Network ==
-== Network ==
-===Consortium members ===
-::::* Dr. Ole Pless - [https://www.ime.fraunhofer.de/en/Research_Divisions/business_fields_TM/screeningport.html Fraunhofer IME, Department ScreeningPort], DE
-::::* Prof. Dr. Frank Edenhofer - [https://www.uibk.ac.at/molbiol/index.html.en Institute for Molecular Biology, University of Innsbruck], AT
-::::* [[Koopman Werner JH| Dr. Werner Koopman]] - [https://www.radboudumc.nl/en/research/departments/biochemistry Radboud University Medical Center, Biochemistry], NL
-::::* Dr. Anu Suomalainen - [https://www.helsinki.fi/en/researchgroups/stem-cells-and-metabolism-research-program University of Helsinki, Research Program of Stem Cells and Metabolism], FI
-::::* Dr. Paweł Lisowski - [http://www.ighz.edu.pl/en/ Institute of Genetics and Animal Biotechnology of the Polish Academy of Sciences, Department of Molecular Biology], PL
-::::* Dr. Emanuela Bottani - [https://www.ddsp.univr.it/?ent=home&page=dipartimento&lang=en Verona University, Department of Diagnostics and Public Health], IT
-::::* Dr. Antonio del Sol - [https://wwwen.uni.lu/lcsb Luxembourg Centre for Systems Biomedicine (LCSB) University of Luxembourg], LU
-::::* Dr. Philip Yeske - [https://www.mitopatients.org/ International Mito Patients (IMP)]
+:::: The Oroboros O2k-Network serves to connect and support: Contact for advice on applications of the O2k and high-resolution respirometry - the global network in mitochondrial physiology.
-[[Image:Fraunhofer Gesellschaft neu.gif‎|110px|Frauenhofer IME]]
+::::: '''»''' The O2k-Network Labs: [[O2k-Network|» O2k-Network]]
-[[Image:University of innsbruck logo.png‎‎|110px|University of Innsbruck]]
-[[Image:Radboudumc logo.png|40px|Radboud University Medical Center]]
-[[Image:University of Helsinki.jpg‎‎‎|70px|University of Helsinki]]
-[[Image:Institute of Genetics and Animal Biotech as PAS logo.png‎‎‎‎‎|160px|Institute of Genetics and Animal Biotechnology of the Polish Academy of Sciences]]
-[[Image:Uni verona logo.png‎|120px|Verona University]]
-[[Image:Lcsb logo.png‎‎|150px|LCSB University of Luxembourg]]
-[[Image:Imp logo.png‎‎‎‎‎|90px|International Mito Patients]]
-=== Collaborators ===
+::::: '''»''' [[O2k-Network_discussion_forum|O2k-Network discussion forum]]
-::::* Prof. Dr. Markus Schuelke - [https://neuropaediatrie.charite.de/ Charité University, Clinic for Pediatrics and Neurology], DE
-::::* Prof. Dr. Felix Distelmaier - [https://www.uniklinik-duesseldorf.de/en/department-of-general-paediatrics Department of General Pediatrics, Neonatology and Pediatric Cardiology, University Children's Hospital, Heinrich Heine University], DE
-::::* Dr. Nael Nadif Kasri - [https://www.radboudumc.nl/en/research/departments/human-genetics Radboud University Medical Centre, Department of Human Genetics], NL
-::::* [[Doerrier Carolina| Dr. Carolina Doerrier]] - [https://www.oroboros.at/ Oroboros Instruments GmbH], AT
-::::* Dr. Valeria Tiranti -  [https://www.istituto-besta.it/ Foundation IRCCS Neurological Institute C. Besta, Unit of Molecular Neurogenetics], IT
-::::* Dr. Dario Brunetti - [http://eng.biometra.unimi.it/ecm/home University of Milan, Department of Medical Biotechnology and Translational Medicine], IT
-::::* Dr. David Meierhofer - [https://www.molgen.mpg.de/2168/en Max Planck Institute for Molecular Genetics], DE
-::::* Prof. Dr. Thomas Klopstock - [http://mitonet.org/en/ Deutsches MITONET e.V., Friedrich-Baur-Institut], DE
-[[Image:Charite logo.png‎|90px|Charité University]]
-[[Image:Hhu logo.png‎‎|110px|Heinrich Heine University]]
-[[Image:Radboudumc logo.png|40px|Radboud University Medical Center]]
-[[Image:Logo OROBOROS INSTRUMENTS.jpg|60px|link=https://www.oroboros.at/ Oroboros]]
-[[Image:Institute besta logo.png‎‎‎|55px|Foundation IRCCS Neurological Institute C. Besta]]
-[[Image:Uni Milano logo.png‎‎‎‎|140px|University of Milan]]
-[[Image:Max Planck Institute for Molecular Genetics logo.png‎‎|50px|Max Planck Institute for Molecular Genetics]]
-[[Image:MitoNET logo.png‎‎‎‎‎‎|120px|Deutsches MITONET e.V.]]
-== Oroboros project involvement ==
+:::: '''»''' [[Oroboros Laboratories|Oroboros O2k-Laboratory]] - open for innovation and cooperation.
-:::: Oroboros is a collaborator on the project providing infrastructure and expertise required to conduct respirometric measurements for the project.
+:::: '''»''' [[Oroboros_Science_Scholarship|Oroboros Science Scholarships]] - are offered for focused research projects on mitochondrial physiology and high-resolution respirometry
-== Support ==
-The EJP RD initiative has received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement N°825575. EJP RD is coordinated by INSERM, France.
-[[File:Flag yellow low.jpg|left|100px]]
-<br/>
+[[Category:O2k-Open Support]]
-<br/>
-<br/>
-* [https://www.ejprarediseases.org/index.php/funded-projects-2020/ Funded projects]