Tissue homogenate: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr= | |abbr=Thom | ||
|description=A '''tissue homogenate''' is obtained through mechanical micro-disruption of fresh tissue and the cell membranes are mechanically permeabilized. | |description=A '''tissue homogenate''' (Thom) is obtained through mechanical micro-disruption of fresh tissue and the cell membranes are mechanically permeabilized. | ||
|info=[[MiPNet17.02 PBI-Shredder-Manual]] | |info=[[MiPNet17.02 PBI-Shredder-Manual]] | ||
|type=Method | |type=Method |
Revision as of 07:32, 2 October 2013
Description
A tissue homogenate (Thom) is obtained through mechanical micro-disruption of fresh tissue and the cell membranes are mechanically permeabilized.
Abbreviation: Thom
Reference: MiPNet17.02 PBI-Shredder-Manual
MitoPedia methods:
Respirometry
Homogenized tissue provides different advantages: the preparation is faster, no detergents (saponin) are required, tissue heterogeneity may entail a statistical problem in application of fibres which is averaged in the homogenate preparation, oxygen limitation is reduced, smaller amounts of tissue are needed compared to isolated mitochondria. Tissue homogenates may be well suited for the study of mitochondrial respiration (Pecinova 2011 Mitochondrion).