Talk:WG1 MitoEAGLE protocols, terminology, documentation
Network discussion forum
- 2018-05-17 Open Call: cytochrome c effect.
I would like to ask you for your feedback related to the cytochrome c effect (test for mtOM integrity) in different mtprep. As you will know, the addition of external cytochrome c during respiratory experiments serves as a quality control for the mitochondrial preparation. Since cytochrome c release can be induced during sample preparation and reflects damage of the sample, it can be used as a criterion for the exclusion of low quality preparations. We observed different flux control factor (FCFc) values for cytochrome c in different mtprep, tissues, and species. Moreover, different substrate combinations could trigger differences in cytochrome c effect. Therefore, in the frame of MitoEAGLE, we would appreciate your contribution sending us your comments and reference values obtained in your own specific model(s) (control group). If you are willing to share any data, we ask you to include if it is possible and available the information of the Word document and send it to [email protected] If not all the information is available or you wish to share the information in a different form, any input is appreciated. Thank you very much for your contribution. We will make all contributions available to the community in this page. Please contact me if you have any comments or questions here: [email protected]ros.at. I hope to hear from you soon. Warmest regards. Carolina Doerrier