Talk:WG1 MitoEAGLE protocols, terminology, documentation
Network discussion forum WG1: cytochrome c effect
- 2018-05-17 Open Call: cytochrome c effect
- I would like to ask you for your feedback related to the cytochrome c effect (test for mitochondrial outer membrane integrity) in different mitochondrial preparations (mtprep). As you know, the addition of external cytochrome c during respiratory experiments serves as a quality control for the mitochondrial preparation. Since cytochrome c release can be induced during sample preparation and reflects damage of the sample, it can be used as a criterion for the exclusion of low quality preparations. We observed different flux control factor values for cytochrome c (FCFc) in different mtprep, tissues, and species. Moreover, different substrate combinations could trigger differences in cytochrome c effect. Therefore, in the frame of MitoEAGLE, we would appreciate your contribution sending us your comments and reference values obtained in your own specific model(s) (control group).
- If you are willing to share any data, we ask you to include if it is possible and available the information of the Word document and send it to [email protected]
- If not all the information is available or you wish to share the information in a different form, any input is appreciated.
- See an example here: FCFc questionnaire in permeabilized muscle fibers (soleus) from mouse
- Cytochrome c effect:
- For harmonizinging our results, please if it is possible, provide us by email the FCFc (median and interquartile range).
- The cytochrome c control factor expresses the control of respiration by externally added cytochrome c, c, as a fractional change of flux from substrate state CHO to CHOc. In this flux control factor (FCFc), CHOc is the reference state with stimulated flux; CHO is the background state with CHO substrates, upon which c is added,
- FCFc = (JCHOc-JCHO)/JCHOc.
- FCFc calculation: = (Oxygen consumption after cytochrome c addition - Oxygen consumption before cytochrome c addition)/ Oxygen consumption after cytochrome c addition.
- Thank you very much for your contribution. We will make all contributions available to the community in this page. Please contact me if you have any comments or questions here: [email protected]
- I hope to hear from you soon. Warmest regards.
- Carolina Doerrier, COST Action 15203 MitoEAGLE WG1 TG leader