Startup O2k-Respirometer: Difference between revisions

Latest revision as of 11:48, 17 August 2022

Startup O2k-Respirometer

MitoPedia O2k and high-resolution respirometry: O2k-Open Support

Description

Startup O2k-Respirometer - the experimental system complete for basic high-resolution respirometry (HRR). The O2k-Respirometer includes the O2k-Main Unit with stainless steel housing, O2k-Assembly Kit, two OroboPOS (polarographic oxygen sensors) and OroboPOS-Service Kit, DatLab software, the ISS-Integrated Suction System, the O2k-Titration Set, and for performing high-resolution respirometry with reduced amounts of biological sample the O2k-sV-Module.

The O2k is a sole source apparatus with no other instruments meeting its specifications.

Reference: Product details and purchase information

Additional resources

» Contents: O2k Manual DatLab 7

» Gnaiger_2008_POS

Chapter	View	Section	Last update
Gnaiger 2008 POS		Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial dysfunction in drug-induced toxicity (Dykens JA, Will Y, eds) John Wiley & Sons, Inc, Hoboken, NJ:327-52.	2008
Gnaiger 2020 BEC MitoPathways	published online 2020-12-30	Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5^th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002	2020
MiPNet04.05 Titration-Injection		From step titration to ramp injection: Uncoupling by FCCP with TIP.	1999-01
MiPNet07.08 User information	» Versions	O2k-Manual: user information. PLEASE STUDY THIS MANUAL.	2017-11-03
MiPNet08.09 CellRespiration	»Versions	High-resolution respirometry and coupling-control protocol with living cells: ROUTINE, LEAK, ET-pathway, ROX.	2016-08-11
MiPNet08.12 IOC22		NO effect on mitochondrial oxygen kinetics at low oxygen. O2k workshop Report.	2011-12-11
MiPNet12.01 Suppl T-issue	»Versions	MitoPathways at the Q-junction: mouse skeletal muscle fibers.	2018-11-18
MiPNet12.11 MitoRespiration	»Versions	Flux control ratios in isolated mitochondria. OXPHOS capacity and respiratory control.	2014-02-20
MiPNet17.03 Shredder vs Fibers	»Versions	Mitochondrial respiration in permeabilized fibers versus homogenate from fish liver and heart. An application study with the PBI-Shredder.	2015-06-18
MiPNet19.18 O2k-Series G Core manual	»Versions	O2k-Core manual contents.	2016-08-08
MiPNet19.18B POS-service	»Versions	Service of the polarographic oxygen sensor OroboPOS.	2021-06-23
MiPNet19.18C DatLab 6: Guide	»Versions	DatLab-guide through the menus.	2016-08-24
MiPNet19.18D O2k-Series G and DatLab 6: Calibration	»Versions	O2k-calibration by DatLab 6.	2016-08-24
MiPNet19.18E O2 flux analysis - DatLab 6	»Versions	Oxygen flux analysis: DatLab real-time.	2016-08-08
MiPNet21.14 Reference sample HRR	»Versions	Development of a reference sample for HRR.	2016-10-19
MiPNet21.16 DatLab 7 Innovations		DatLab 7: innovations.	2019-09-23
MiPNet22.16 DatLab 7 Innovations: DL-Protocols	» Versions	DatLab 7.4 Innovations: DL-Protocols	2019-09-13
MiPNet23.12 DL-Protocols help	» Versions	DatLab Protocols: Help DatLab 7.	2019-09-13
MiPNet24.06 Oxygen flux analysis - DatLab 7.4	» Versions	Oxygen flux analysis with DatLab 7.4.	2020-04-16
MiPNet24.08 Safranin Analysis Template	» Versions	Excel template for safranin data analysis.	2020-05-13
MiPNet24.09 Data analysis of mt-membrane potential	» Versions	Data analysis of mt-membrane potential.	2021-09-16
MiPNet25.14 TPP Analysis Template	» [https:.pdf Versions]	Excel template for TPP data analysis.	2020-##-##
MiPNet26.06 DatLab 7: Guide	»Versions	DatLab-guide through the menus.	2021-06-02
MiPNet28.10 SmartPOS-service	» Versions	SmartPOS: Service	2023-09-29
Pesta 2012 Methods Mol Biol	PMID: 22057559	Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscle. Methods Mol Biol 810:25-58. https://doi.org/10.1007/978-1-61779-382-0_3	2012

MitoPedia methods: Respirometry

MitoPedia O2k and high-resolution respirometry: O2k hardware, O2k-Open Support, O2k-Respirometry

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+{{Technical support}}
+__TOC__
+{{MitoPedia without banner
+|abbr=
+|description=[[Image:Startup O2k-Respirometer notitle.png|200px|right]]'''Startup O2k-Respirometer''' - the experimental system complete for basic [[high-resolution respirometry]] (HRR). The O2k-Respirometer includes the [[O2k-Main Unit]] with stainless steel housing, [[O2k-Assembly Kit]], two [[OroboPOS]] (polarographic oxygen sensors) and [[OroboPOS-Service Kit]], [[DatLab]] software, the [[ISS-Integrated Suction System]], the [[O2k-Titration Set]], and for performing high-resolution respirometry with reduced amounts of biological sample the [[O2k-sV-Module]].
-{{Product
-|description='''Startup O2k-Respirometer''' - the experimental system complete for basic [[High-Resolution Respirometry|high-resolution respirometry]] (HRR). The O2k-Respirometer includes the [[O2k-Main Unit]] with stainless steel housing, [[O2k-Assembly Kit]], two [[OroboPOS]] (polarographic oxygen sensors) and [[OroboPOS-Service Kit]], [[DatLab]] software, the [[ISS-Integrated Suction System]] and the [[O2k-Titration Set]]. The O2k-Respirometer supports all add-on O2k-Modules of the [[Oroboros O2k]], and is a component of the [[O2k-FluoRespirometer]].
-On-line display of oxygen flux (rate of respiration) is provided in addition to the conventional 'oxygraphic' plot of oxygen concentration over time. Highest signal stability minimizes the required amounts of biological sample, and provides the basis for resolution in the extreme low-oxygen range. Peltier temperature control provides a thermal stability at ±0.002 °C in the range of 4 °C to 47 °C at typical constant room temperature. Electronically controlled PVDF or PEEK stirrers are integrated in the two-chamber design of the O2k, and a barometric pressure transducer enables automatic oxygen calibrations implemented in the DatLab software.
+The O2k is a sole source apparatus with no other instruments meeting its [[MiPNet06.05 Test Experiments on O2k-Specifications | specifications]].
+|info= [https://www.oroboros.at/index.php/product/startup-o2k-respirometer/ '''Product details and purchase information''']
-The O2k is a sole source apparatus with no other instruments meeting its [[MiPNet06.05 Specifications | specifications]].
-|product id=10022-02
-|product type=O2k
-|info=[[Gnaiger_2008_POS]]
-|product image=[[Image:Startup O2k-Respirometer notitle.png|200px|right]]
 }}
-&nbsp;[[File:Startup O2k-Respirometer.png|right|600px|O2k-FluoRespirometer]]
+[[File:Startup O2k-Respirometer.png|right|400px|O2k-FluoRespirometer]]
-:
-::
-:::
-::::
-::::*The '''O2k-Core''' does not include, but fully supports and is required for the add-on Modules of the [[Oroboros_O2k|Oroboros O2k]], such as the [[Titration-Injection_microPump|TIP2k]], [[O2k-Fluo_LED2-Module|O2k-Fluo LED2-Module]], [[O2k-TPP+_ISE-Module|O2k-TPP+ ISE-Module]], [[O2k-pH_ISE-Module|O2k-pH ISE-Module]], [[O2k-NO_Amp-Module|O2k-NO Amp-Module]].
-:
-::
-:::
-::::
-::::*A PC is required (not included), connecting DatLab to the O2k via a USB port. For PC requirements, see [[DatLab#PC_requirements|here]].
-[[File:Questions.jpg|left|40px|Questions.jpg]]
-<div class="toccolours mw-collapsible mw-collapsed">
-:
-::
-:::<span style="font-size:105%;  color:##424242">'''» Keywords'''</span>
-<div class="mw-collapsible-content">
-:
-::
-:::'''Specific'''
-::::» [[DatLab|DatLab]]
-::::» [[DatLab#PC_requirements|DatLab PC requirements]]
-::::» [[ISS-Integrated_Suction_System|ISS-Integrated Suction System]]
-::::» [[Oroboros_O2k|Oroboros O2k]]
-::::» [[O2k-Assembly_Kit|O2k-Assembly Kit]]
-::::» [[O2k-Main_Unit|O2k-Main Unit]]
-::::» [[O2k-Titration_Set|O2k-Titration Set]]
-::::» [[OroboPOS|OroboPOS]]
-::::» [[OroboPOS-Service_Kit|OroboPOS-Service Kit]]
-::::» [[MiPNet06.05_Specifications|Oxygraph specifications]]
-:
-::
-:::'''General'''
-::::» [[O2k-FluoRespirometer|O2k-FluoRespirometer]]
-::::» [[O2k-Fluo_LED2-Module|O2k-Fluo LED2-Module]]
-::::» [[High-Resolution FluoRespirometry|O2k high-resolution respirometry]]
-::::» [[O2k-NO_Amp-Module|O2k-NO Amp-Module]]
-::::» [[O2k-pH_ISE-Module|O2k-pH ISE-Module]]
-::::» [[O2k-TPP+_ISE-Module|O2k-TPP+ ISE-Module]]
-::::» [[Titration-Injection_microPump|TIP2k]]
-</div> </div>
-&nbsp;
-== The '''Startup O2k-Respirometer''' ''consists of'' ==
+== Additional resources ==
+:::: » [[O2k-Manual#O2k-FluoRespirometer_.28Series_H.29_and_DatLab_7|Contents: O2k Manual DatLab 7]] <br>
+:::: » [[Gnaiger_2008_POS]]
-{{#ask:[[Category:Products]] [[Product type::Startup O2k-Respirometer]]
-| ?description
-| ?product id
-| ?product image
-|sort=product id
-|order=ascending
-|mainlabel=Title
-}}
-== O2k-Core manual and selected protocols ==
-:
-::
-:::
-::::» <big>'''[[O2k-Manual#O2k-FluoRespirometer_.28Series_H.29_and_DatLab_7|Contents: O2k Manual DatLab 7]]'''</big>
 {{#ask:[[Category:Publications]] [[Additional label::O2k-Core]]
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+{{MitoPedia methods
+|mitopedia method=Respirometry
+}}
 {{MitoPedia O2k and high-resolution respirometry
-|mitopedia O2k and high-resolution respirometry=O2k hardware
+|mitopedia O2k and high-resolution respirometry=O2k hardware, O2k-Open Support, O2k-Respirometry
 }}