Difference between revisions of "SUIT-018"
From Bioblast
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{{MitoPedia | {{MitoPedia | ||
|abbr=NS(GM) | |abbr=NS(GM) | ||
|description= | |description=[[File:SUIT-018 AmR mt D031.png|200px|SUIT-018]] | ||
|info='''A''' [[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/6/64/SUIT-018.pdf|Bioblast pdf]] ยป[http://wiki.oroboros.at/index.php/File:SUIT-018.pdf Versions] | |info='''A''' [[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/6/64/SUIT-018.pdf|Bioblast pdf]] ยป[http://wiki.oroboros.at/index.php/File:SUIT-018.pdf Versions] | ||
}} | }} | ||
::: '''[[Categories of SUIT protocols |SUIT-category]]:'''NS(GM) | ::: '''[[Categories of SUIT protocols |SUIT-category]]:'''NS(GM) | ||
::: '''[[SUIT protocol pattern]]:''' linear 1GMS;2D; | ::: '''[[SUIT protocol pattern]]:''' linear 1GMS;2D;- | ||
__TOC__ | __TOC__ | ||
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== References == | == References == | ||
ย | {{#ask:[[Category:Publications]] [[Additional label::SUIT-018]] [[Additional label::SUIT-018 AmR mt D031]]ย [[Instrument and method::O2k-Protocol]] | ||
| ?Was published in year=Year | |||
| ?Has title=Reference | |||
| ?Mammal and model=Organism | |||
| ?Tissue and cell=Tissue;cell | |||
| format=broadtable | |||
| limit=5000 | |||
| offset=0 | |||
| sort=Was published in year | |||
| order=descending | |||
}} | |||
{{Template:SUIT-018}} | {{Template:SUIT-018}} | ||
== Strengths and limitations == | == Strengths and limitations == | ||
ย | :::+ Reasonable duration of the experiment. | ||
:::+ Simple protocol to evaluate the oxygen dependence of H<sub>2</sub>O<sub>2</sub> production. | |||
:::- Measurements with Amplex UltraRed assay cannot be carried out with liver homogenate. | |||
:::- H<sub>2</sub>O<sub>2</sub> production can be studied in two different states, LEAK and OXPHOS. | |||
:::- Reoxygenation and nitrogen injection could lead to bubble formation in the O2k-Chamber | |||
Revision as of 18:13, 24 January 2019
Description
Abbreviation: NS(GM)
Reference: A ยปVersions
- SUIT-category:NS(GM)
- SUIT protocol pattern: linear 1GMS;2D;-
DLP applications
- SUIT-018 AmR mt D031 simultaneous determination of O2 and H2O2 flux on isolated mitochondria and tissue homogenate.
References
Year | Reference | Organism | Tissue;cell | |
---|---|---|---|---|
MiPNet24.10 H2O2 flux analysis | 2021-10-22 | Hydrogen peroxide flux analysis using Amplex UltraRed assay in MiR05-Kit with DatLab 7.4 | ||
Komlodi 2021 BEC AmR-O2 | 2021 | Komlรณdi T, Sobotka O, Gnaiger E (2021) Facts and artefacts on the oxygen dependence of hydrogen peroxide production using Amplex UltraRed. Bioenerg Commun 2021.4. https://doi.org/10.26124/bec:2021-0004 | Saccharomyces cerevisiae | Other cell lines |
MiPNet20.14 AmplexRed H2O2-production | 2019-06-24 | O2k-FluoRespirometry: HRR and simultaneous determination of H2O2 production with Amplex UltraRed. | Mouse | Heart |
Komlodi 2018 Methods Mol Biol | 2018 | Komlodi T, Sobotka O, Krumschnabel G, Bezuidenhout N, Hiller E, Doerrier C, Gnaiger E (2018) Comparison of mitochondrial incubation media for measurement of respiration and hydrogen peroxide production. Methods Mol Biol 1782:137-55. | Human Mouse | Skeletal muscle HEK |
Makrecka-Kuka 2015 Biomolecules | 2015 | Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. https://doi.org/10.3390/biom5031319 | Human Mouse | Heart Nervous system HEK |
Krumschnabel 2015 Methods Mol Biol | 2015 | Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61. | Mouse | Nervous system |
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
0DTPA |
| |||
0SOD |
| |||
0HRP |
| |||
0AmR |
|
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
1GMS | GMSL(n) | NS | CI&CII | 1GMS
|
2D | GMSP | NS | CI&CII | 1GMS;2D
|
3Ama | ROX | 1GMS;2D;3Ama
|
Strengths and limitations
- + Reasonable duration of the experiment.
- + Simple protocol to evaluate the oxygen dependence of H2O2 production.
- - Measurements with Amplex UltraRed assay cannot be carried out with liver homogenate.
- - H2O2 production can be studied in two different states, LEAK and OXPHOS.
- - Reoxygenation and nitrogen injection could lead to bubble formation in the O2k-Chamber
Compare SUIT protocols
MitoPedia concepts: MiP concept, SUIT protocol, Recommended
MitoPedia methods:
Respirometry