Difference between revisions of "SUIT-003 Ce1;ce2SD;ce3U;ce4Rot;ce5Ama"
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{{MitoPedia | |||
|abbr=CCP_R 1S 2U | |||
|description=[[File:CCP_R 1S 2U.jpg|right|200px]] | |||
|info=[[Steinlechner-Maran 1997 Transplantation]] | |||
}} | |||
{{MitoPedia concepts | |||
|mitopedia concept=SUIT protocol | |||
}} | |||
::: '''[[Categories of SUIT protocols |SUIT-catg]]:''' CCP(S) | |||
::: '''[[SUIT protocol pattern]]:''' complex [[coupling control protocol]] (CCP) | |||
::: '''[[Mark names in DatLab]]:''' CCP(S)01 | |||
::: '''[[DatLab-Excel templates |DatLab-Excel template]]:''' ''in prep.'': CCP(S)01.xlsx | |||
== Coupling control protocol == | |||
{| class="wikitable" border="1" | |||
|- | |||
! Step | |||
! Respiratory state | |||
! Pathway control | |||
! Comment | |||
|- | |||
| R | |||
| [[ROUTINE]] | |||
| [[Intact cells |ce]] | |||
| Intact cells, ce, are in the ROUTINE state of respiration. [[Permeabilized tissue or cells |pce]] without substrate are in a state of ROX. | |||
|- | |||
| 1S | |||
| ''R''+S<sub>''L''</sub> | |||
| [[ROUTINE]] (''R'') + [[S]] (S<sub>''L''</sub>) | |||
| pce are in state S<sub>''L''</sub>. ADP should be added to stimulate pce to the OXPHOS state ([[CCP(S)02]]). | |||
|- | |||
| 2U | |||
| ''E''+S<sub>''E''</sub> | |||
| [[ETS]] (''E'') + [[S]] (S<sub>''E''</sub>) | |||
| ce and pce are in the ETS state. | |||
|- | |||
| 3Rot | |||
| ROX+S<sub>''E''</sub> | |||
| [[ROX]]+[[S]] (S<sub>''E''</sub>) | |||
| ce are in the ROX state, pce are in the ETS state. | |||
|- | |||
| 4Ama | |||
| ROX<sub>Ama</sub> | |||
| [[ROX]] | |||
| | |||
|} | |||
Revision as of 14:27, 3 September 2016
- high-resolution terminology - matching measurements at high-resolution
SUIT-003 Ce1;ce2SD;ce3U;ce4Rot;ce5Ama
Description
Abbreviation: CCP_R 1S 2U
Reference: Steinlechner-Maran 1997 Transplantation
MitoPedia concepts:
SUIT protocol
- SUIT-catg: CCP(S)
- SUIT protocol pattern: complex coupling control protocol (CCP)
- Mark names in DatLab: CCP(S)01
- DatLab-Excel template: in prep.: CCP(S)01.xlsx
Coupling control protocol
| Step | Respiratory state | Pathway control | Comment |
|---|---|---|---|
| R | ROUTINE | ce | Intact cells, ce, are in the ROUTINE state of respiration. pce without substrate are in a state of ROX. |
| 1S | R+SL | ROUTINE (R) + S (SL) | pce are in state SL. ADP should be added to stimulate pce to the OXPHOS state (CCP(S)02). |
| 2U | E+SE | ETS (E) + S (SE) | ce and pce are in the ETS state. |
| 3Rot | ROX+SE | ROX+S (SE) | ce are in the ROX state, pce are in the ETS state. |
| 4Ama | ROXAma | ROX |
