Oroboros Laboratory: Difference between revisions
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Revision as of 16:16, 11 December 2015
Oroboros Laboratory
The MitoFit Centre of Excellence
OROBOROS INSTRUMENTS collaborates closely with research labs of the Medical University of Innsbruck, the Ludwig-Franzens University of Innsbruck, and with visiting scientists from numerous internation research institutions.
According to the concept of open innovation, the Mitochondrial Research Laboratory welcomes visiting researchers from all over the world.
MitoFit project
- Florian Hoppel, MSc. - [email protected]
- PhD student
- Institute for Sports Science and OROBOROS: O2k-MitoFit
Oncotyrol project
The Oncotyrol research project (project leader: A.Univ.Prof.Mag.Dr.Helmut Klocker) has its focus on functional differences of mitochondria in human prostate tumor versus benign tissue.
- Bernd Schoepf B, Mag. - [email protected]
- PhD student, Oncotyrol project
O2k instruments
24 O2k-Chambers (twelve Oxygraphs)
- Eight Oxygraph-2k instruments (16 chambers) equipped with automatic Titration-Injection microPumps and the newest O2k-MultiSensor Modules are available for scientific research and routine biomedical applications
- Two O2ks are used for specific testing
- Two O2ks are devoted to instrumental development
More details: OROBOROS Support
The OROBOROS Mitochondrial Research Laboratory (MitoFit Lab) is a scientific research laboratory in Innsbruck, Austria, that is probably the world-wide best equipped lab for high-resolution respirometry. The MitoFit Lab was opened during the course of the K-Regio project MitoCom Tyrol, starting in June 2013. It comprises 140 sqm which are divided into the office and research areas.
SUIT protocols are applied in tissue samples from animal models and human patients. Using cultured cells or cells extracted from blood samples, the effects of genotypic variations or of various drugs are studied on OXPHOS parameters, using
- a phosphorylation control protocol in intact cells to evaluate effects on ROUTINE respiration, biochemical coupling efficiency and extracellular ROS production under physiological conditions
- various substrate-uncoupler-inhibitor titration (SUIT) protocols in permeabilized cells for deep OXPHOS phenotyping (cell ergometry)
