Oroboros Laboratory: Difference between revisions

Revision as of 16:06, 11 December 2015

Oroboros Laboratory

The MitoFit Centre of Excellence

OROBOROS INSTRUMENTS collaborates closely with research labs of the Medical University of Innsbruck, the Ludwig-Franzens University of Innsbruck, and with visiting scientists from numerous internation research institutions.

According to the concept of open innovation, the Mitochondrial Research Laboratory welcomes visiting researchers from all over the world.

Visiting scientists

MitoFit project

Florian Hoppel, MSc. - [email protected]

PhD student

Institute for Sports Science and OROBOROS: O2k-MitoFit

Oncotyrol project

The Oncotyrol research project (project leader: A.Univ.Prof.Mag.Dr.Helmut Klocker) has its focus on functional differences of mitochondria in human prostate tumor versus benign tissue.

Bernd Schoepf B, Mag. - [email protected]

PhD student, Oncotyrol project

O2k instruments

24 O2k-Chambers (twelve Oxygraphs)

Eight Oxygraph-2k instruments (16 chambers) equipped with automatic Titration-Injection microPumps and the newest O2k-MultiSensor Modules are available for scientific research and routine biomedical applications

Two O2ks are used for specific testing

Two O2ks are devoted to instrumental development

More details: OROBOROS Support

The OROBOROS Mitochondrial Research Laboratory (MitoFit Lab) is a scientific research laboratory in Innsbruck, Austria, that is probably the world-wide best equipped lab for high-resolution respirometry. The MitoFit Lab was opened during the course of the K-Regio project MitoCom Tyrol, starting in June 2013. It comprises 140 sqm which are divided into the office and research areas.

SUIT protocols are applied in tissue samples from animal models and human patients. Using cultured cells or cells extracted from blood samples, the effects of genotypic variations or of various drugs are studied on OXPHOS parameters, using

a phosphorylation control protocol in intact cells to evaluate effects on ROUTINE respiration, biochemical coupling efficiency and extracellular ROS production under physiological conditions

various substrate-uncoupler-inhibitor titration (SUIT) protocols in permeabilized cells for deep OXPHOS phenotyping (cell ergometry)

@@ Line 1: / Line 1: @@
 {{Template:OROBOROS header page name}}
+== The MitoFit Centre of Excellence ==
-The OROBOROS MitoLab is a scientific research laboratory in Innsbruck, Austria, that is probably the world-wide best equipped lab for high-resolution respirometry. The OROBOROS MitoLab was started in June 2013 and comprises 140 sqm, which are divided into an office area and the state of the art research area with 24 O2k-Chambers (twelve Oxygraphs):
+OROBOROS INSTRUMENTS collaborates closely with research labs of the Medical University of Innsbruck, the Ludwig-Franzens University of Innsbruck, and with visiting scientists from numerous internation research institutions.
-* Eight Oxygraph-2k instruments (16 chambers) equipped with automatic Titration-Injection microPumps and the newest O2k-MultiSensor Modules are available for scientific research and routine biomedical applications
+According to the concept of open innovation, the Mitochondrial Research Laboratory welcomes visiting researchers from all over the world.
+[[OROBOROS_MitFit_Lab:_visiting_scientists| Visiting scientists]]
-* Two O2ks are used for specific testing
+[[File:ORO Lab 1.jpg|250px]] [[File:ORO Lab 2.jpg|250px]] [[File:ORO Lab 3.jpg|250px]] [[File:ORO Lab 4.jpg|250px]]
-* Two O2ks are devoted to instrumental development
+=== MitoFit project ===
+[[Image:HoppelF.jpg|left|90px]]
-For further information please click here: [[O2k-technical_support_and_open_innovation|OROBOROS Support]]
+:::: '''[[Hoppel F|Florian Hoppel]]''', MSc. - [mailto:[email protected] [email protected]]
+:::: PhD student
+:::: Institute for Sports Science and OROBOROS: [[O2k-MitoFit]]
+<br /> <br /> <br /> <br />
+=== Oncotyrol project ===
+The [[Oncotyrol| Oncotyrol]] research project (project leader: A.Univ.Prof.Mag.Dr.Helmut Klocker) has its focus on functional differences of mitochondria in human prostate tumor versus benign tissue.
+[[Image:SchoepfB.jpg|left|90px]]
-The OROBOROS Mitochondrial Research Laboratory was opened during the course of the K-Regio project [[MitoCom_O2k-Fluorometer| MitoCom Tyrol]]. Currently, the laboratory accommodates an [[Oncotyrol| Oncotyrol]] research project (project leader: A.Univ.Prof.Mag.Dr.Helmut Klocker) with focus on functional differences of mitochondria in human prostate tumor versus benign tissue.
+:::: '''[[Schoepf B|Bernd Schoepf B]]''', Mag. - [mailto:bernd.schoepf@student.i-med.ac.at [email protected].at]
+:::: PhD student, [http://oroboros.at/index.php?id=oncotyrol Oncotyrol] project
+== O2k instruments ==
+'''24 O2k-Chambers (twelve Oxygraphs)'''
-OROBOROS INSTRUMENTS collaborates closely with research labs of the Medical University of Innsbruck. SUIT protocols are applied in tissue samples from animal models and human patients. Using cultured cells or cells extracted from blood samples, the effects of genotypic variations or of various drugs are studied on OXPHOS parameters, using
+* Eight Oxygraph-2k instruments (16 chambers) equipped with automatic Titration-Injection microPumps and the newest O2k-MultiSensor Modules are available for scientific research and routine biomedical applications
+* Two O2ks are used for specific testing
+* Two O2ks are devoted to instrumental development
-* a phosphorylation control protocol in intact cells to evaluate effects on ROUTINE respiration, biochemical coupling efficiency and extracellular ROS production under physiological conditions
-* various substrate-uncoupler-inhibitor titration (SUIT) protocols in permeabilized cells for deep OXPHOS phenotyping ([[Cell_ergometry|cell ergometry]])
+More details: [[O2k-technical_support_and_open_innovation|OROBOROS Support]]
-According to the concept of open innovation, the Mitochondrial Research Laboratory welcomes visiting researchers from all over the world.
+The OROBOROS Mitochondrial Research Laboratory (MitoFit Lab) is a scientific research laboratory in Innsbruck, Austria, that is probably the world-wide best equipped lab for high-resolution respirometry. The MitoFit Lab was opened during the course of the K-Regio project [[MitoCom_O2k-Fluorometer| MitoCom Tyrol]], starting in June 2013. It comprises 140 sqm which are divided into the office and research areas.
+SUIT protocols are applied in tissue samples from animal models and human patients. Using cultured cells or cells extracted from blood samples, the effects of genotypic variations or of various drugs are studied on OXPHOS parameters, using
-[[OROBOROS_MitoLab:_visiting_scientists| Visiting scientists]]
+* a phosphorylation control protocol in intact cells to evaluate effects on ROUTINE respiration, biochemical coupling efficiency and extracellular ROS production under physiological conditions
-[[File:ORO Lab 1.jpg|250px]] [[File:ORO Lab 2.jpg|250px]] [[File:ORO Lab 3.jpg|250px]] [[File:ORO Lab 4.jpg|250px]]
+* various substrate-uncoupler-inhibitor titration (SUIT) protocols in permeabilized cells for deep OXPHOS phenotyping ([[Cell_ergometry|cell ergometry]])