Difference between revisions of "NextGen-O2k Scientific developments"
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== Progress and next steps == | == Progress and next steps == | ||
:::: '''2020-05-25:''' Project partner WGT begins designing a new type of sensor tip that protects the LEDs and helps with the maintenance of the PB-Sensors | |||
:::: '''2020-04-24:''' First tests with pH electrodes | |||
:::: '''2020-03-11:''' First tests with [[Calcium Green]] | |||
:::: '''2020-02-20:''' Started development of protocols (and DL-Protocols) for the PB-Module, including new [[SUIT]] protocols for photosynthesis measurements. | |||
:::: '''2020-02-11:''' First prototype of NextGen-O2k (Q3-001) with integrated PB-Module received for in-house testing | |||
:::: '''2020-02-11:''' First prototypes of the PB-Sensors received | |||
:::: '''2019-12-31:''' Determination of optimal Q-sensor measurement conditions for different biological samples (brain and heart isolated mitochondria from mouse, yeast) | |||
:::: '''2019-12-06:''' First version of the Cyclic Voltammetry Manual released: [[MiPNet24.16 DatLab8.0: CV-Module |CV-Module]] | |||
:::: '''2019-12-02:''' Optimization of O2k-Procedures for isolated mitochondria preparations | |||
:::: '''2019-11-22:''' First version of NextGen-O2k Manual/SOP finalized: [[MiPNet24.12 NextGen-O2k: Q-Module |NextGen-O2k: Q-Module]] | |||
:::: '''2019-11-21:''' Updated beta version of cyclic voltammetry provided to the Key Opinion Leaders for testing | |||
:::: '''2019-11-18:''' Application of Q-redox state measurements in scientific projects | |||
:::: '''2019-10-22:''' 66 [[SUIT]] protocols for different O2k-Applications (i.e. oxygen consumption, hydrogen peroxide production, mitochondrial membrane potential, ATP production) which can be measured in living cells, permeabilized cells, isolated mitochondria, tissue homogenate and permeabilized fibers optimized and released with DatLab 7.4, see [[MitoPedia:_SUIT]] | |||
:::: '''2019-10-21:''' Four new videos for DatLab 7.4 software released - see: [https://wiki.oroboros.at/index.php/O2k-Videosupport#DatLab_7.4 O2k-Videosupport DatLab 7.4] | |||
:::: '''2019-09-24:''' Two new videos for mitochondrial preparations released - see: [https://wiki.oroboros.at/index.php/O2k-Videosupport#O2k-Mitochondrial_preparations O2k-Videosupport Mitochondrial preparations] | |||
:::: '''2019-09-23:''' First beta version of the cyclic voltammetry used by the Key Opinion Leaders during 1<sup>st </sup>Q-Module workshop at the [[MiPNet24.02 IOC141 Schroecken AT |Oroboros O2k-Workshop IOC141]] | |||
:::: '''2019-09-20:''' First six videos for the Q-Module released - see: [https://wiki.oroboros.at/index.php/O2k-Videosupport#Q-Module_.28NextGen-O2k_-_DatLab_7.29 O2k-Videosupport Q-Module] | |||
:::: '''2019-09-06:''' Development of two harmonized [[SUIT]] protocols (and DL-Protocols) for the Q-Module, both provided to the Key Opinion Leaders | |||
:::: '''2019-07-17:''' First tests of the new [[MiPNet24.06 Oxygen flux analysis - DatLab 7.4| O2 analysis template DL7.4]] | |||
:::: '''2019-07:''' Started development of [[SUIT]] protocols for isolated mitochondria and permeabilzied cells | |||
:::: '''2019-06-18:''' Testing of the [[SUITbrowser]] by the participants at the Oroboros O2k-Workshop on high-resolution respirometry (HRR), [[MiPNet24.01 IOC139 Schroecken AT| IOC139]] | |||
:::: '''2019-06:''' Integration of cyclic voltammetry as a quality control step into the Q-Module | |||
:::: '''2019-06:''' Final version of the specific Q-Stopper (with integrated electronics) | |||
:::: '''2019-06:''' Testing of the [[SUITbrowser]] | |||
== Links and references == | == Links and references == | ||
Revision as of 14:31, 16 June 2020
Template:MitoFit highlights page name
The revolutionary all-in-one instrument to conquer mitochondrial disease.
WP2: Scientific developments
Abstract
- The aim of this workpackage is to test measurement conditions for the newly developed modules and the development of specific applications.
- Determination of optimal Q-sensor measurement conditions
- Determination of optimal measurement conditions for the PB-Module
- Development of protocols for scientific, pre-clinical and algal biotechnology applications
- Data handling and quality control
- The aim of this workpackage is to test measurement conditions for the newly developed modules and the development of specific applications.
Progress and next steps
- 2020-05-25: Project partner WGT begins designing a new type of sensor tip that protects the LEDs and helps with the maintenance of the PB-Sensors
- 2020-04-24: First tests with pH electrodes
- 2020-03-11: First tests with Calcium Green
- 2020-02-20: Started development of protocols (and DL-Protocols) for the PB-Module, including new SUIT protocols for photosynthesis measurements.
- 2020-02-11: First prototype of NextGen-O2k (Q3-001) with integrated PB-Module received for in-house testing
- 2020-02-11: First prototypes of the PB-Sensors received
- 2019-12-31: Determination of optimal Q-sensor measurement conditions for different biological samples (brain and heart isolated mitochondria from mouse, yeast)
- 2019-12-06: First version of the Cyclic Voltammetry Manual released: CV-Module
- 2019-12-02: Optimization of O2k-Procedures for isolated mitochondria preparations
- 2019-11-22: First version of NextGen-O2k Manual/SOP finalized: NextGen-O2k: Q-Module
- 2019-11-21: Updated beta version of cyclic voltammetry provided to the Key Opinion Leaders for testing
- 2019-11-18: Application of Q-redox state measurements in scientific projects
- 2019-10-22: 66 SUIT protocols for different O2k-Applications (i.e. oxygen consumption, hydrogen peroxide production, mitochondrial membrane potential, ATP production) which can be measured in living cells, permeabilized cells, isolated mitochondria, tissue homogenate and permeabilized fibers optimized and released with DatLab 7.4, see MitoPedia:_SUIT
- 2019-10-21: Four new videos for DatLab 7.4 software released - see: O2k-Videosupport DatLab 7.4
- 2019-09-24: Two new videos for mitochondrial preparations released - see: O2k-Videosupport Mitochondrial preparations
- 2019-09-23: First beta version of the cyclic voltammetry used by the Key Opinion Leaders during 1st Q-Module workshop at the Oroboros O2k-Workshop IOC141
- 2019-09-20: First six videos for the Q-Module released - see: O2k-Videosupport Q-Module
- 2019-09-06: Development of two harmonized SUIT protocols (and DL-Protocols) for the Q-Module, both provided to the Key Opinion Leaders
- 2019-07-17: First tests of the new O2 analysis template DL7.4
- 2019-07: Started development of SUIT protocols for isolated mitochondria and permeabilzied cells
- 2019-06-18: Testing of the SUITbrowser by the participants at the Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC139
- 2019-06: Integration of cyclic voltammetry as a quality control step into the Q-Module
- 2019-06: Final version of the specific Q-Stopper (with integrated electronics)
- 2019-06: Testing of the SUITbrowser
