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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
MitoEAGLE data: blood and cultured cells
WG4
- MITOEAGLE data repository for blood cells and clutured cells
WG4 Description
- Biopsy sampling of human muscle and fat tissue for mitochondrial studies is an invasive approach. More recently measurement of mitochondrial function in human blood cells – as well as temporary cryopreservation (Karabatsiakis 2014 Transl Psychiatry) – has become an attractive and by far less invasive alternative for mitochondrial diagnostics. Mitochondrial function of blood cells was linked with numerous diseases and metabolic conditions such as sepsis, neurodegenerative diseases, depression, hypertension, amyotrophic lateral sclerosis, and early-stage heart failure (e.g. Sjoevall 2013 Crit Care; Karabatsiakis 2014 Transl Psychiatry; Hagl 2014 J Alzheimer Dis). The use of human blood cells will thus predictably widen the applicability of respirometry in human physiology and will provide a significant stimulus for the entire field of mitochondrial medicine.
- A key towards achieving comparability among data sets and the applicability of human blood cells for the evaluation of mitochondrial fitness in health and disease is the standardization of the procedures to separate or isolate blood cells, the experimental procedure for the evaluation of mitochondrial respiratory characteristics, and the format for reporting. A consensus on protocols should be reached in WG4, calling for a Workshop right at the start of the COST Action. Depending on the WG4 participants input, cell lines will be defined for which harmonization of datasets should be achieved (Fig. 2).
WG4 Participants
Country | City | Contact |
---|---|---|
Armenia | Yerevan | Trchounian K |
Austria | Innsbruck | Hoppel Florian |
Austria | Innsbruck | Fischer Christine |
Austria | Innsbruck | Meszaros Andras T |
Austria | Innsbruck | Gnaiger Erich |
Austria | Innsbruck | Torres-Quesada Omar |
Austria | Innsbruck | Garcia-Souza Luiz F |
Austria | Innsbruck | Doerrier Carolina |
Austria | Innsbruck | Cardoso Luiza HD |
Austria | Innsbruck | Karabatsiakis Alexander |
Belgium | Namur | Detraux Damien |
Belgium | Leuven | Bird Matthew |
Belgium | Namur | Arnould Thierry |
Belgium | Liege | Votion Dominique-Marie |
Belgium | Namur | Renard P |
Bosnia and Herzegovina | Tuzla | Hajrulahovic Anesa |
Bosnia and Herzegovina | Tuzla | Jusic Amela |
Brazil | Sao Paulo | Verdaguer Ignasi Bofill |
Brazil | Sao Paulo | Dominguez-Amorocho OA |
Brazil | Sao Paulo | Alencar Mayke Bezerra |
Brazil | São Paulo | Crispim Marcell |
Croatia | Zagreb | Rubelj Ivica |
Czech Republic | Pilsen | Kuncova J |
Czech Republic | Hradec Kralove | Vernerova Andrea |
Czech Republic | Hradec Kralove | Maseko Tumisang Edward |
Czech Republic | Prague 4 | Bajzikova Martina |
Czech Republic | Prague | Pecina Petr |
Czech Republic | Prague | Mracek Tomas |
Czech Republic | Vestec | Zobalova Renata |
Czech Republic | Prague | Hansikova Hana |
Czech Republic | Pilsen | Kripnerova M |
Czech Republic | Pilsen | Markova Michaela |
Czech Republic | Prague | Fisar Zdenek |
Czech Republic | Prague | Elkalaf Moustafa |
Czech Republic and Australia | Prague and Southport | Neuzil Jiri |
Czech rep | Hradec Kralove | Falaye T |
Denmark | Copenhagen | Rasmussen LJ |
Denmark | Copenhagen N | Mortensen OH |
Denmark | Copenhagen | Desler C |
Denmark | Hvidovre | Nehlin Jan O |
Denmark | Aarhus | Rattan Suresh IS |
Denmark | Malov | Hey-Mogensen M |
Egypt | Cairo | Abdel-Rahman Engy Ali |
Egypt | Cairo | Ali Sameh S |
Estonia | Tallinn | Truu Laura |
Estonia | Tallinn | Klepinina Lyudmila |
Estonia | Tartu | Grellier T |
Finland | Helsinki | Pirinen E |
Finland | Jyvaeskylae | Lehti Maarit |
Finland | Turku | Stier Antoine |
France | Paris | Petit Patrice X |
France | Bordeaux cedex | Mourier A |
France | Bron | Rizwan Alam M |
Germany | Ulm | Hartmann C |
Germany | Neuherberg | Borchard Sabine |
Germany | Ulm | Kolassa Iris |
Germany | Freising | Klingenspor Martin |
Germany | Leipzig | Ost Mario |
Germany | Gießen | Silaidos Carmina |
Germany | Düsseldorf | Prigione Alessandro |
Germany | Regensburg | Renner-Sattler Kathrin |
Germany | Duesseldorf | Piel Sarah |
Germany | Freising | Keppner Gloria |
Germany | Giessen | Eckert Gunter P |
Germany | Freising | Fromme Tobias |
Germany | Mainz | Vujacic-Mirski Ksenija |
Germany | Munich | Jastroch M |
Germany | Biberach an der Riß | Nold V |
Germany | Hamburg | Singer Dominique |
Germany | Mainz | Kalinovic S |
Germany | Magdeburg | Vieira Ligo Teixeira Camila |
Germany | Freising | Schoettl T |
Germany | Duesseldorf | Eckers A |
Germany | Ulm | Calzia Enrico |
Germany | Ulm | Schaefer PM |
Germany | Freising-Weihenstephan | Musiol Eva |
Greece | Athens | Trougakos Ioannis P |
Hungary | Debrecen | Verebne Tar K |
Hungary | Budapest | Tretter Laszlo |
Hungary | Budapest | Komlodi Timea |
Ireland | Dublin | Porter Richard K |
Ireland | Sligo | Murphy J |
Ireland | Dublin | Karavyraki Marilena |
Israel | Rishon Le Zion | Hachmo Yafit |
Israel | Jerusalem | Saada Reisch Ann |
Israel | Zrifin | Sova Marina |
Italy | Padova | Fernandez-Vizarra Erika |
Italy | Milano | Bottoni P |
Italy | Bolzano | Castelo Rueda Maria Paulina |
Italy | Verona | Calabria Elisa |
Italy | Bologna | Genova Maria Luisa |
Italy | Bolzano | Pichler Irene |
Italy | Bolzano/Bozen | Volani Chiara |
Italy | Bologna | Paterlini S |
Italy | Ancona | Battino Maurizio |
Italy | Bologna | Porcelli AM |
Italy | Bologna | Gasparre G |
Italy | Rome | Scatena Roberto |
Japan | Sapporo | Shirakawa R |
Latvia | Riga | Makarova Elina |
Latvia | Riga | Kidere Dita |
Malaysia | Kuala Lumpur | Abdul Karim Norwahidah |
Malta | Msida | Vella Joanna |
Netherlands | Nijmegen | Koopman Werner JH |
Netherlands | Wageningen | Keijer Jaap |
Netherlands | Groningen | Rots MG |
Norway | Oslo | Eide L |
Norway | Oslo | Lund Jenny |
Norway | Oslo | Nikolic N |
Norway | Oslo | Rustan AC |
Norway | Bergen | Tronstad Karl Johan |
Palestine | Ramallah | Stiban Johnny |
Poland | Poznan | Rybacka-Mossakowska Joanna |
Poland | Lodz | Watala Cezary |
Poland | Lodz | Labieniec-Watala Magdalena |
Poland | Lodz | Siewiera Karolina |
Poland | Krakow | Kaczara P |
Poland | Poznan | Michalak Slawomir |
Poland | Warsaw | Wasilewski M |
Poland | Poznań | Ludziejewska A |
Poland | Poznan | Swiniuch Daria |
Poland | Warsaw | Dymkowska Dorota |
Poland | Warsaw | Kampa Rafal Pawel |
Poland | Warsaw | Sek Aleksandra |
Portugal | Coimbra | Pereira Susana P |
Portugal | Porto | Bernardino RL |
Portugal | Porto | Alves Marco G |
Portugal | Cantanhede | Pinho Sonia A |
Portugal | Catanhede | Pereira da Silva Grilo da Silva Filomena |
Portugal | Coimbra | Carvalho Eugenia |
Portugal | Porto | Beleza Jorge |
Portugal | Porto | Carrageta D |
Portugal | Porto | Magalhaes Jose |
Portugal | Porto | Martins Ana Dias |
Portugal | Porto | Crisostomo Luis |
Portugal | Porto | Moreira Bruno P |
Portugal | Coimbra | Palmeira Carlos |
Portugal | Cantanhede | Bento Guida Jose Freitas |
Portugal | Porto | Oliveira Pedro Fontes |
Portugal | Covilhã | Dias Tania R |
Republic of Korea | Yuseong-gu Daejeon | Quang Don T |
Republic of Korea | Daejeon | Park SH |
Republic of Serbia | Belgrade | Pavlovic Kasja |
Republic of Serbia | Belgrade | Krako Jakovljevic Nina |
Romania | Timisoara | Lighezan R |
Romania | Timisoara | Lelcu Theia |
Romania | Timisoara | Betiu AM |
Romania | Timisoara | Avram Vlad F |
Romania | Timisoara | Muntean Danina M |
Romania | Timisoara | Danila Maria-Daniela |
Russia | Moscow | Itkis YS |
Russia | Moscow | Tsygankova P |
Russia | Moscow | Krylova T |
Saudi Arabia | Giza | Kotb IA |
Serbia | Belgrade | Vukojevic M |
Serbia | Belgrad | Markovic Ivanka |
Serbia | Belgrade | Misirlic-Dencic S |
Serbia | Belgrade | Isakovic A |
Serbia | Belgrade | Ivanovic Tijana |
Serbia | Belgrade | Jeremic M |
Slovakia | Bratislava | Sumbalova Zuzana |
Slovakia | Bratislava | Nemec Michal |
Slovakia | Košice | Cizmarova Beata |
Slovakia | Bratislava | Ukropec J |
Slovakia | Bratislava | Ukropcova B |
Slovakia | Bratislava | Maderova D |
Slovenia | Ljubljana | Pirkmajer Sergej |
Slovenia | Ljubljana | Mars T |
Slovenia | Ljubljana | Kopitar-Jerala Natasa |
Spain | Barcelona | Juarez D |
Spain | Valencia | Fuertes Agudo Marina |
Spain | Sevilla | Muntane Jordi |
Spain | Barcelona | Zorzano Antonio |
Spain | A Coruña | Mayan MD |
Spain | Madrid | De los Rios C |
Spain | Barcelona | Gama Perez Pau |
Spain | Sevilla | Bustos Matilde |
Spain | Salamanca | Almeida Angeles |
Spain | Barcelona | Tura Ceide O |
Spain | Armilla, Granada | Escames Germaine |
Spain | Barcelona | Arago Marc |
Spain | Madrid | Cano-Abad MF |
Spain | Barcelona | Hyrossova Petra |
Spain | A Coruña | Blanco FJ |
Spain | Armilla (Granada) | Fernandez-Ortiz Marisol |
Spain | Barcelona | Smolders V |
Spain | Madrid | Monsalve Maria |
Spain | Barcelona | Gil J |
Spain | Madrid | Lopez MG |
Spain | Valencia | Victor Victor Manuel |
Spain | Ourense | Reboredo-Rodriguez Patricia |
Spain | Madrid | Cadenas S |
Spain | Lleida | Gonzalo Hugo |
Spain | Barcelona | Gonzalez Casacuberta I |
Spain | Valencia | Borras Consuelo |
Spain | Lleida | Nogueras L |
Spain | Barcelona | Dahdah Norma Ramon |
Spain | Valencia | Casado Pinna Marta |
Spain | Granada | Quiles JL |
Sweden | Linköping | Fontes-Oliveira CC |
Sweden | Lund | Elmer Eskil |
Sweden | Lund | Chamkha Imen |
Sweden | Södertälje | Alfaro-Moreno E |
Sweden | Lund | Westerlund E |
Sweden | Lund | Simon Serrano Sonia |
Sweden | Lund | Leffler M |
Sweden | Lund | Karlsson M |
Sweden | Lund | Abliva |
Sweden, SE | Lund | Aasander Frostner Eleonor |
Switzerland | Geneva | Martinou JC |
Switzerland | Lausanne | Zanou Nadege |
The Netherlands | BT Amsterdam | Wuest Rob CI |
Turkey | Tekirdag | Aral Cenk |
Turkey | Izmir | Aydin HH |
Turkey | Ankara | Engin Ayse Basak |
Turkey | Ankara | Tuncay Erkan |
Turkey | Istanbul | Baykal AT |
USA | Philadelphia | Starr Jonathan |
USA | Philadelphia | Janowska Joanna |
USA | St Louis | Welch R |
USA | Iowa City | Buettner Garry R |
USA | Fayetteville | Rao RR |
USA | Philadelphia | Kilbaugh Todd |
USA | Orlando | Coen Paul M |
USA | Baton Rouge | Irving Brian A |
USA | Fayetteville | Iyer Shilpa |
United Kingdom | Brighton | Moore Anthony L |
United Kingdom | Oxford | Makrecka-Kuka Marina |
United Kingdom | Stoke-on-Trent | McStay Gavin P |
United Kingdom | Leicester | Moisoi Nicoleta |
United Kingdom | Birmingham | Thakker A |
United Kingdom | Birmingham | Joanisse S |
Uruguay | Montevideo | Radi R |
WG4 Management
Tasks
- Kick-off meeting to develop a working plan for the establishment of consensus protocols, reporting schemes, and the assignment of specific study tasks.
- Development of SOPs for cell separation.
- Development of laboratory protocols for individual blood cell types (and other cell types) for mitochondrial studies on intact and permeabilized cells.
- Application of SOPs in experimental studies during Short Term Scientific Missions and feeding of data into the MITOEAGLE data repository.
- Publication of SOPs and study results.
Milestones
- Consensus on protocols, reporting schemes and work assignments.
- Completed SOPs for cell preparation & laboratory protocols.
- Application study finished and data transmitted to MITOEAGLE data repository.
- Publication finished.
Deliverables
- SOPs for blood cell separation and respirometric characterisation open for the research community.
- MITOEAGLE data repository for comparative data evaluation, planning of future studies, data mining.
- Publication with a set of reference data.
Feedback WG4
- Dr. Ali will work within the realms of WG4 (blood and cultured cells) and potentially WG1 (protocol development) as well as WG5 (dissemination and training). He will participate in data generation, quality control, and repository efforts of protocols recommended by these working groups. Dr. Ali will contribute to training workshops and conferences that aim at defining standard procedures and terminology in the field of mitochondria physiology. - Sameh S Ali (2016).
- I am very interested in being part of the management committee either as a MC member for managment tasks or in working group 4 MITOEAGLE data repository for blood cells and cultured cells. I have experience in analyzing mitochondrial function in different human tissues as cultured cells and blood cells (also subsets of specific blood populations, see list of publications), muscle and isolated mitochondria. This together with my experience in organizing summer schools and conferences could be helpful as MC member taking care of management tasks. However, if you think my expertise is more helpful in working group 4, I am looking forward to support this working group. - Kathrin Renner-Sattler (2016).