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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
MitoEAGLE data: blood and cultured cells
WG4
- MITOEAGLE data repository for blood cells and clutured cells
WG4 Description
- Biopsy sampling of human muscle and fat tissue for mitochondrial studies is an invasive approach. More recently measurement of mitochondrial function in human blood cells – as well as temporary cryopreservation (Karabatsiakis 2014 Transl Psychiatry) – has become an attractive and by far less invasive alternative for mitochondrial diagnostics. Mitochondrial function of blood cells was linked with numerous diseases and metabolic conditions such as sepsis, neurodegenerative diseases, depression, hypertension, amyotrophic lateral sclerosis, and early-stage heart failure (e.g. Sjoevall 2013 Crit Care; Karabatsiakis 2014 Transl Psychiatry; Hagl 2014 J Alzheimer Dis). The use of human blood cells will thus predictably widen the applicability of respirometry in human physiology and will provide a significant stimulus for the entire field of mitochondrial medicine.
- A key towards achieving comparability among data sets and the applicability of human blood cells for the evaluation of mitochondrial fitness in health and disease is the standardization of the procedures to separate or isolate blood cells, the experimental procedure for the evaluation of mitochondrial respiratory characteristics, and the format for reporting. A consensus on protocols should be reached in WG4, calling for a Workshop right at the start of the COST Action. Depending on the WG4 participants input, cell lines will be defined for which harmonization of datasets should be achieved (Fig. 2).
WG4 Participants
Country | City | Contact |
---|---|---|
Armenia | Yerevan | Trchounian K |
Austria | Innsbruck | Doerrier Carolina |
Austria | Innsbruck | Torres-Quesada Omar |
Austria | Innsbruck | Karabatsiakis Alexander |
Austria | Innsbruck | Hoppel Florian |
Austria | Innsbruck | Cardoso Luiza HD |
Austria | Innsbruck | Gnaiger Erich |
Austria | Innsbruck | Fischer Christine |
Austria | Innsbruck | Garcia-Souza Luiz F |
Austria | Innsbruck | Meszaros Andras T |
Belgium | Namur | Renard P |
Belgium | Liege | Votion Dominique-Marie |
Belgium | Namur | Detraux Damien |
Belgium | Namur | Arnould Thierry |
Belgium | Leuven | Bird Matthew |
Bosnia and Herzegovina | Tuzla | Jusic Amela |
Bosnia and Herzegovina | Tuzla | Hajrulahovic Anesa |
Brazil | Sao Paulo | Dominguez-Amorocho OA |
Brazil | Sao Paulo | Alencar Mayke Bezerra |
Brazil | São Paulo | Crispim Marcell |
Brazil | Sao Paulo | Verdaguer Ignasi Bofill |
Croatia | Zagreb | Rubelj Ivica |
Czech Republic | Prague | Hansikova Hana |
Czech Republic | Prague | Fisar Zdenek |
Czech Republic | Pilsen | Kuncova J |
Czech Republic | Pilsen | Kripnerova M |
Czech Republic | Prague | Pecina Petr |
Czech Republic | Prague 4 | Bajzikova Martina |
Czech Republic | Prague | Mracek Tomas |
Czech Republic | Prague | Elkalaf Moustafa |
Czech Republic | Hradec Kralove | Vernerova Andrea |
Czech Republic | Hradec Kralove | Maseko Tumisang Edward |
Czech Republic | Vestec | Zobalova Renata |
Czech Republic | Pilsen | Markova Michaela |
Czech Republic and Australia | Prague and Southport | Neuzil Jiri |
Czech rep | Hradec Kralove | Falaye T |
Denmark | Malov | Hey-Mogensen M |
Denmark | Copenhagen N | Mortensen OH |
Denmark | Hvidovre | Nehlin Jan O |
Denmark | Copenhagen | Rasmussen LJ |
Denmark | Aarhus | Rattan Suresh IS |
Denmark | Copenhagen | Desler C |
Egypt | Cairo | Abdel-Rahman Engy Ali |
Egypt | Cairo | Ali Sameh S |
Estonia | Tartu | Grellier T |
Estonia | Tallinn | Klepinina Lyudmila |
Estonia | Tallinn | Truu Laura |
Finland | Turku | Stier Antoine |
Finland | Helsinki | Pirinen E |
Finland | Jyvaeskylae | Lehti Maarit |
France | Bordeaux cedex | Mourier A |
France | Paris | Petit Patrice X |
France | Bron | Rizwan Alam M |
Germany | Gießen | Silaidos Carmina |
Germany | Düsseldorf | Prigione Alessandro |
Germany | Freising | Schoettl T |
Germany | Biberach an der Riß | Nold V |
Germany | Ulm | Calzia Enrico |
Germany | Freising | Fromme Tobias |
Germany | Duesseldorf | Eckers A |
Germany | Mainz | Vujacic-Mirski Ksenija |
Germany | Ulm | Schaefer PM |
Germany | Mainz | Kalinovic S |
Germany | Magdeburg | Vieira Ligo Teixeira Camila |
Germany | Freising | Klingenspor Martin |
Germany | Leipzig | Ost Mario |
Germany | Regensburg | Renner-Sattler Kathrin |
Germany | Duesseldorf | Piel Sarah |
Germany | Freising-Weihenstephan | Musiol Eva |
Germany | Ulm | Hartmann C |
Germany | Freising | Keppner Gloria |
Germany | Giessen | Eckert Gunter P |
Germany | Neuherberg | Borchard Sabine |
Germany | Munich | Jastroch M |
Germany | Hamburg | Singer Dominique |
Germany | Ulm | Kolassa Iris |
Greece | Athens | Trougakos Ioannis P |
Hungary | Budapest | Komlodi Timea |
Hungary | Debrecen | Verebne Tar K |
Hungary | Budapest | Tretter Laszlo |
Ireland | Dublin | Karavyraki Marilena |
Ireland | Dublin | Porter Richard K |
Ireland | Sligo | Murphy J |
Israel | Rishon Le Zion | Hachmo Yafit |
Israel | Zrifin | Sova Marina |
Israel | Jerusalem | Saada Reisch Ann |
Italy | Bari | Agrimi G |
Italy | Bologna | Porcelli AM |
Italy | Ancona | Battino Maurizio |
Italy | Milano | Bottoni P |
Italy | Bologna | Gasparre G |
Italy | Rome | Scatena Roberto |
Italy | Verona | Calabria Elisa |
Italy | Bologna | Genova Maria Luisa |
Italy | Bolzano | Pichler Irene |
Italy | Padova | Fernandez-Vizarra Erika |
Italy | Bolzano | Castelo Rueda Maria Paulina |
Italy | Bolzano/Bozen | Volani Chiara |
Italy | Bologna | Paterlini S |
Japan | Sapporo | Shirakawa R |
Latvia | Riga | Makarova Elina |
Latvia | Riga | Kidere Dita |
Malaysia | Kuala Lumpur | Abdul Karim Norwahidah |
Malta | Msida | Vella Joanna |
Netherlands | Groningen | Rots MG |
Netherlands | Wageningen | Keijer Jaap |
Netherlands | Nijmegen | Koopman Werner JH |
Norway | Oslo | Nikolic N |
Norway | Oslo | Rustan AC |
Norway | Oslo | Lund Jenny |
Norway | Bergen | Tronstad Karl Johan |
Norway | Oslo | Eide L |
Palestine | Ramallah | Stiban Johnny |
Poland | Poznań | Ludziejewska A |
Poland | Lodz | Watala Cezary |
Poland | Poznan | Swiniuch Daria |
Poland | Lodz | Labieniec-Watala Magdalena |
Poland | Warsaw | Kampa Rafal Pawel |
Poland | Warsaw | Sek Aleksandra |
Poland | Lodz | Siewiera Karolina |
Poland | Poznan | Rybacka-Mossakowska Joanna |
Poland | Krakow | Kaczara P |
Poland | Poznan | Michalak Slawomir |
Poland | Warsaw | Wasilewski M |
Poland | Warsaw | Dymkowska Dorota |
Portugal | Porto | Magalhaes Jose |
Portugal | Coimbra | Palmeira Carlos |
Portugal | Porto | Carrageta D |
Portugal | Porto | Martins Ana Dias |
Portugal | Porto | Crisostomo Luis |
Portugal | Porto | Moreira Bruno P |
Portugal | Cantanhede | Bento Guida Jose Freitas |
Portugal | Porto | Oliveira Pedro Fontes |
Portugal | Covilhã | Dias Tania R |
Portugal | Coimbra | Carvalho Eugenia |
Portugal | Porto | Bernardino RL |
Portugal | Porto | Alves Marco G |
Portugal | Coimbra | Pereira Susana P |
Portugal | Cantanhede | Pinho Sonia A |
Portugal | Porto | Beleza Jorge |
Portugal | Catanhede | Pereira da Silva Grilo da Silva Filomena |
Republic of Korea | Daejeon | Park SH |
Republic of Korea | Yuseong-gu Daejeon | Quang Don T |
Republic of Serbia | Belgrade | Krako Jakovljevic Nina |
Republic of Serbia | Belgrade | Pavlovic Kasja |
Romania | Timisoara | Muntean Danina M |
Romania | Timisoara | Betiu AM |
Romania | Timisoara | Avram Vlad F |
Romania | Timisoara | Lighezan R |
Romania | Timisoara | Danila Maria-Daniela |
Romania | Timisoara | Lelcu Theia |
Russia | Moscow | Itkis YS |
Russia | Moscow | Tsygankova P |
Russia | Moscow | Krylova T |
Saudi Arabia | Giza | Kotb IA |
Serbia | Belgrad | Markovic Ivanka |
Serbia | Belgrade | Misirlic-Dencic S |
Serbia | Belgrade | Jeremic M |
Serbia | Belgrade | Isakovic A |
Serbia | Belgrade | Ivanovic Tijana |
Serbia | Belgrade | Vukojevic M |
Slovakia | Bratislava | Ukropec J |
Slovakia | Košice | Cizmarova Beata |
Slovakia | Bratislava | Sumbalova Zuzana |
Slovakia | Bratislava | Ukropcova B |
Slovakia | Bratislava | Maderova D |
Slovakia | Bratislava | Nemec Michal |
Slovenia | Ljubljana | Pirkmajer Sergej |
Slovenia | Ljubljana | Mars T |
Slovenia | Ljubljana | Kopitar-Jerala Natasa |
Spain | Barcelona | Smolders V |
Spain | Armilla (Granada) | Fernandez-Ortiz Marisol |
Spain | Barcelona | Gil J |
Spain | Valencia | Victor Victor Manuel |
Spain | Barcelona | Gonzalez Casacuberta I |
Spain | Ourense | Reboredo-Rodriguez Patricia |
Spain | Valencia | Casado Pinna Marta |
Spain | Lleida | Gonzalo Hugo |
Spain | Barcelona | Zorzano Antonio |
Spain | Granada | Quiles JL |
Spain | Barcelona | Juarez D |
Spain | Lleida | Nogueras L |
Spain | Armilla, Granada | Escames Germaine |
Spain | A Coruña | Mayan MD |
Spain | Valencia | Borras Consuelo |
Spain | Barcelona | Dahdah Norma Ramon |
Spain | Madrid | De los Rios C |
Spain | A Coruña | Blanco FJ |
Spain | Barcelona | Gama Perez Pau |
Spain | Valencia | Fuertes Agudo Marina |
Spain | Sevilla | Muntane Jordi |
Spain | Madrid | Cano-Abad MF |
Spain | Salamanca | Almeida Angeles |
Spain | Madrid | Cadenas S |
Spain | Barcelona | Tura Ceide O |
Spain | Madrid | Monsalve Maria |
Spain | Sevilla | Bustos Matilde |
Spain | Madrid | Lopez MG |
Spain | Barcelona | Arago Marc |
Spain | Barcelona | Hyrossova Petra |
Sweden | Södertälje | Alfaro-Moreno E |
Sweden | Lund | Karlsson M |
Sweden | Lund | Westerlund E |
Sweden | Lund | Elmer Eskil |
Sweden | Lund | Simon Serrano Sonia |
Sweden | Linköping | Fontes-Oliveira CC |
Sweden | Lund | Leffler M |
Sweden | Lund | Abliva |
Sweden | Lund | Chamkha Imen |
Sweden, SE | Lund | Aasander Frostner Eleonor |
Switzerland | Lausanne | Zanou Nadege |
Switzerland | Geneva | Martinou JC |
The Netherlands | BT Amsterdam | Wuest Rob CI |
Turkey | Ankara | Tuncay Erkan |
Turkey | Istanbul | Baykal AT |
Turkey | Tekirdag | Aral Cenk |
Turkey | Izmir | Aydin HH |
Turkey | Ankara | Engin Ayse Basak |
USA | Iowa City | Buettner Garry R |
USA | Fayetteville | Iyer Shilpa |
USA | Philadelphia | Starr Jonathan |
USA | Fayetteville | Rao RR |
USA | St Louis | Welch R |
USA | Philadelphia | Janowska Joanna |
USA | Philadelphia | Kilbaugh Todd |
USA | Orlando | Coen Paul M |
USA | Baton Rouge | Irving Brian A |
United Kingdom | Birmingham | Thakker A |
United Kingdom | Birmingham | Joanisse S |
United Kingdom | Stoke-on-Trent | McStay Gavin P |
United Kingdom | Oxford | Makrecka-Kuka Marina |
United Kingdom | Leicester | Moisoi Nicoleta |
United Kingdom | Brighton | Moore Anthony L |
Uruguay | Montevideo | Radi R |
WG4 Management
Tasks
- Kick-off meeting to develop a working plan for the establishment of consensus protocols, reporting schemes, and the assignment of specific study tasks.
- Development of SOPs for cell separation.
- Development of laboratory protocols for individual blood cell types (and other cell types) for mitochondrial studies on intact and permeabilized cells.
- Application of SOPs in experimental studies during Short Term Scientific Missions and feeding of data into the MITOEAGLE data repository.
- Publication of SOPs and study results.
Milestones
- Consensus on protocols, reporting schemes and work assignments.
- Completed SOPs for cell preparation & laboratory protocols.
- Application study finished and data transmitted to MITOEAGLE data repository.
- Publication finished.
Deliverables
- SOPs for blood cell separation and respirometric characterisation open for the research community.
- MITOEAGLE data repository for comparative data evaluation, planning of future studies, data mining.
- Publication with a set of reference data.