MiR05: Difference between revisions
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|description='''Mitochondrial respiration medium, MiR05''', developed for oxygraph incubations of [[mitochondrial preparations]]. Respiration of [[living cells]] may be assessed in MiR05 by adding pyruvate (P) as an external source.ย [[MiR06]] = MiR05 + catalase. | |description='''Mitochondrial respiration medium, MiR05''', developed for oxygraph incubations of [[mitochondrial preparations]]. Respiration of [[living cells]] may be assessed in MiR05 by adding pyruvate (P) as an external source.ย [[MiR06]] = MiR05 + catalase. | ||
[[MiR05Cr]] = [[MiR05]] + creatine. | [[MiR05Cr]] = [[MiR05]] + creatine. | ||
|info=[ | |info=[https://www.oroboros.at/index.php/product/mir05-kit/ MiR05-Kit], [[Gnaiger 2000 Life in the Cold]] | ||
}} | }} | ||
== Comparison of respiration media == | == Comparison of respiration media == | ||
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== MiR05-Kit in HRR == | == MiR05-Kit in HRR == | ||
::::* For preparation of MiR05, see [[MiR05- | ::::* For preparation of MiR05, see [[MiPNet22.10 MiR05-kit]]. | ||
::::* The '''[[oxygen solubility factor]]''' of MiR05 is '''0.92'''. | ::::* The '''[[oxygen solubility factor]]''' of MiR05 is '''0.92'''. | ||
Revision as of 12:07, 11 August 2020
Description
Mitochondrial respiration medium, MiR05, developed for oxygraph incubations of mitochondrial preparations. Respiration of living cells may be assessed in MiR05 by adding pyruvate (P) as an external source. MiR06 = MiR05 + catalase. MiR05Cr = MiR05 + creatine.
Abbreviation: MiR05
Reference: MiR05-Kit, Gnaiger 2000 Life in the Cold
Comparison of respiration media
- Gnaiger E, Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Steurer W, Margreiter R (2000) Mitochondria in the cold. In: Life in the Cold (Heldmaier G, Klingenspor M, eds) Springer, Heidelberg, Berlin, New York:431-42. - ยปBioblast linkยซ - The original article describing MiR05 in comparison with the composition of mitochondrial and organ preservation solutions.
- Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61. - ยปBioblast linkยซ
- Wollenman LC, Vander Ploeg MR, Miller ML, Zhang Y, Bazil JN (2017) The effect of respiration buffer composition on mitochondrial metabolism and function. PLOS ONE 12:e0187523. - ยปBioblast linkยซ - "when the goal is to maximize ADP-stimulated respiration, buffers containing K-lactobionate or K-gluconate are superior choices compared to the standard KCl-based buffers".
- Komlรณdi T, Sobotka O, Krumschnabel G, Bezuidenhoutz N, Hiller E, Doerrier C, Gnaiger E (2018) Comparison of mitochondrial incubation media for measurement of respiration and hydrogen peroxide production. Methods Mol Biol 1782:137-55. - ยปBioblast linkยซ
- Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mรฉszรกros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Methods Mol Biol 1782:31-70. - ยปBioblast linkยซ
MiR05-Kit in HRR
- For preparation of MiR05, see MiPNet22.10 MiR05-kit.
- The oxygen solubility factor of MiR05 is 0.92.
MitoPedia topics:
Media for respirometry