Difference between revisions of "MiPNet23.04 IOC133 Innsbruck AT"
Beno Marija (talk | contribs) |
Beno Marija (talk | contribs) |
||
(20 intermediate revisions by 5 users not shown) | |||
Line 1: | Line 1: | ||
{{OROBOROS header page name}} | {{OROBOROS header page name}} | ||
{{Publication | {{Publication | ||
|title= [[Image:OROBOROS-solo.png|right|60px|link= | |title= [[Image:OROBOROS-solo.png|right|60px|link=Oroboros Instruments]] | ||
'''Innsbruck AT''', 2018 Sep 05-07. MitoFit Coaching Days - Basic '''IOC'''. | '''Innsbruck AT''', 2018 Sep 05-07. MitoFit Coaching Days - Basic '''IOC 133'''. | ||
|info= | |info= | ||
|authors=Oroboros | |authors=Oroboros | ||
Line 9: | Line 9: | ||
|abstract=[[Image:O2k-Workshops.png|left|130px|link=O2k-Workshops]] | |abstract=[[Image:O2k-Workshops.png|left|130px|link=O2k-Workshops]] | ||
'''MitoFit Coaching Days - Basic'''. Innsbruck, Austria; 2018 Sep 05-07. | '''MitoFit Coaching Days - Basic'''. Innsbruck, Austria; 2018 Sep 05-07. | ||
|mipnetlab= | |mipnetlab=AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit | ||
}} | }} | ||
Line 19: | Line 19: | ||
== General information == | == General information == | ||
::::MitoFit Coaching Days - Basic provide a fundamental introduction to | ::::MitoFit Coaching Days - Basic provide a fundamental introduction to high-resolution respirometry with the Oroboros O2k. Hands-on sessions range from OroboPOS technical service to respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols. | ||
== Venue == | == Venue == | ||
Line 47: | Line 47: | ||
|- | |- | ||
|17:00 - 18:00 | |17:00 - 18:00 | ||
|Welcome - | |Welcome - Get-together: Introduction of participants and their research interests | ||
|MiP''Art'' | |MiP''Art'' | ||
|- | |- | ||
Line 59: | Line 59: | ||
|- | |- | ||
|09:00 | |09:00 | ||
| | |OroboPOS service and O2k instrumental setup - overview with videoclips | ||
| | |ORO-MitoFit Lab | ||
|- | |||
|09:30 | |||
|'''Hands-on''': OroboPOS service and O2k instrumental setup | |||
|ORO-MitoFit Lab | |||
|- | |- | ||
|10: | |10:10 | ||
| | |'''Hands-on''': DatLab 7 | ||
|ORO-MitoFit Lab | |||
|- | |- | ||
|10:30 | |10:30 | ||
|'''Hands-on''': | |''Coffee'' | ||
| | | | ||
|- | |||
|11:00 | |||
|Instrumental quality control 1: oxygen calibration | |||
|ORO-MitoFit Lab | |||
|- | |||
|11:30 | |||
|'''Hands-on''': Instrumental quality control 1: oxygen calibration | |||
DL-Protocol: O2k-cleaning before use | |||
DL-Protocol: O2 calibration air | |||
|ORO-MitoFit Lab | |||
|- | |- | ||
|12:00 | |12:00 | ||
Line 75: | Line 91: | ||
|- | |- | ||
|13:00 | |13:00 | ||
|Instrumental quality control 1: oxygen calibration | |'''Hands-on''':Instrumental quality control 1: oxygen calibration (continuation) | ||
| | DL-Protocol: O2 calibration air | ||
|ORO-MitoFit Lab | |||
|- | |||
|13:30 | |||
|Instrumental quality control 2: Instrumental O2 background – overview with videoclips | |||
|ORO-MitoFit Lab | |||
|- | |- | ||
|14:00 | |14:00 | ||
|Instrumental quality control 2: Instrumental O2 background | |'''Hands-on''':Instrumental quality control 2: Instrumental O2 background | ||
| | DL-Protocol: Instrumental O2 background TiP2k | ||
|ORO-MitoFit Lab | |||
|- | |- | ||
|15:00 | |15:00 | ||
Line 99: | Line 121: | ||
|- | |- | ||
|09:00 | |09:00 | ||
|Introduction to SUIT protocols RP1&RP2 | |Hands-on: Instrumental quality control 1: oxygen calibration | ||
| | |||
DL-Protocol: O2k-cleaning before use | |||
DL-Protocol: O2 calibration air | |||
|ORO-MitoFit Lab | |||
|- | |||
|09:15 | |||
|Application of the O2k Series H | |||
|ORO-MitoFit Lab | |||
|- | |||
|09:30 | |||
|Introduction to substrate-uncoupler-inhibitor titration (SUIT) protocols – fundamental principles. SUIT reference protocol: RP1&RP2 | |||
|ORO-MitoFit Lab | |||
|- | |- | ||
|10:00 | |10:00 | ||
Line 107: | Line 141: | ||
|- | |- | ||
|10:30 | |10:30 | ||
|O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2. | |'''Hands-on''': O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2. | ||
| | |||
DL-Protocol: SUIT-1_O2_pce_D03.DLP | |||
DL-Protocol: SUIT-2 O2 pce D07.DLP | |||
|ORO-MitoFit Lab | |||
|- | |||
|11:45 | |||
|'''Hands-on''': O2k-cleaning after use | |||
DL-Protocol: O2k-cleaning after use | |||
|ORO-MitoFit Lab | |||
|- | |- | ||
|12:00 | |12:00 | ||
Line 115: | Line 160: | ||
|- | |- | ||
|13:00 | |13:00 | ||
|'''Hands-on''': O2k-cleaning after use | |||
DL-Protocol: O2k-cleaning after use | |||
|ORO-MitoFit Lab | |||
|- | |||
|13:30 | |||
|Introduction to DatLab analysis | |Introduction to DatLab analysis | ||
| | |ORO-MitoFit Lab | ||
|- | |- | ||
|15:00 | |15:00 | ||
Line 135: | Line 186: | ||
|- | |- | ||
|09:00 | |09:00 | ||
| | |'''MitoFit proficiency test:''' | ||
'''Hands-on''': Instrumental quality control 1: oxygen calibration | |||
DL-Protocol: O2k-cleaning before use | |||
DL-Protocol: O2 calibration air | |||
|ORO-MitoFit Lab | |||
|- | |- | ||
|10: | |10:00 | ||
|''Coffee'' | |''Coffee'' | ||
| | | | ||
|- | |- | ||
| | |10:30 | ||
| | |'''MitoFit proficiency test:''' | ||
| | |||
'''Hands-on''': O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2. | |||
DL-Protocol: SUIT-1_O2_pce_D03.DLP | |||
DL-Protocol: SUIT-2 O2 pce D07.DLP | |||
|ORO-MitoFit Lab | |||
|- | |||
|11:45 | |||
|'''Hands-on''': O2k-cleaning after use | |||
DL-Protocol: O2k-cleaning after use | |||
|ORO-MitoFit Lab | |||
|- | |- | ||
|12:00 | |12:00 | ||
Line 151: | Line 222: | ||
|- | |- | ||
|13:00 | |13:00 | ||
|O2k- | |'''Hands-on''': O2k-cleaning after use | ||
| | |||
DL-Protocol: O2k-cleaning after use | |||
|ORO-MitoFit Lab | |||
|- | |||
|13:15 | |||
|DatLab analysis | |||
|ORO-MitoFit Lab | |||
|- | |- | ||
|14:00 | |14:00 | ||
|DatLab analysis | |DatLab analysis summary | ||
|ORO-MitoFit Lab | |ORO-MitoFit Lab | ||
|- | |- | ||
Line 163: | Line 240: | ||
|- | |- | ||
|15:30 | |15:30 | ||
| | |The Bioblast wiki, O2k-Network and MITOEAGLE | ||
| | |MiP''Art'' | ||
|- | |- | ||
|16:15 | |16:15 | ||
| | |Feedback & conclusions | ||
|MiP''Art'' | |MiP''Art'' | ||
|- | |- | ||
|17: | |17:00 | ||
|Farewell activity | |Farewell activity | ||
|MiP''Art'' | |MiP''Art'' | ||
Line 189: | Line 266: | ||
| '''AT_Innsbruck_Schwarzer C''' - Medical University, Innsbruck (AT) | | '''AT_Innsbruck_Schwarzer C''' - Medical University, Innsbruck (AT) | ||
|- | |- | ||
| [[File: | | [[File:CardosoLHD.JPG|70px|Participant]] | ||
| [[Cardoso LHD|Daltro Cardoso Luiza Helena]] | | [[Cardoso LHD|Daltro Cardoso Luiza Helena]] | ||
| '''[[AT Innsbruck Oroboros]]''' - Oroboros Instruments, Innsbruck (AT) | | '''[[AT Innsbruck Oroboros]]''' - Oroboros Instruments, Innsbruck (AT) | ||
|- | |- | ||
| [[File:Profile-icon-9.png|70px|Participant]] | | [[File:Profile-icon-9.png|70px|Participant]] | ||
| [[Chowdhury SK|Chowdhury Subir]] | | [[Chowdhury SK|Roy Chowdhury Subir]] | ||
| '''CA Winnipeg Banerji V''' - University of Manitoba, Winnipeg (CA) | | '''[[CA Winnipeg Banerji V]]''' - University of Manitoba, Winnipeg (CA) | ||
|- | |- | ||
| [[File: | | [[File:GalleeL.JPG|70px|Participant]] | ||
| [[Gallee Leon]] | |||
| '''[[AT Innsbruck Oroboros]]''' - Oroboros Instruments, Innsbruck (AT) | |||
|- | |||
| | |||
| [[Garipi Enis]] | | [[Garipi Enis]] | ||
| '''[[AT Innsbruck Oroboros]]''' - Oroboros Instruments, Innsbruck (AT) | | '''[[AT Innsbruck Oroboros]]''' - Oroboros Instruments, Innsbruck (AT) | ||
|- | |- | ||
| [[File: | | [[File:Huete-OrtegaM.JPG|70px|Participant]] | ||
| [[Huete-Ortega M|Huete-Ortega Maria]] | | [[Huete-Ortega M|Huete-Ortega Maria]] | ||
| '''[[AT Innsbruck Oroboros]]''' - Oroboros Instruments, Innsbruck (AT) | | '''[[AT Innsbruck Oroboros]]''' - Oroboros Instruments, Innsbruck (AT) | ||
Line 222: | Line 303: | ||
:::: Oroboros Instruments | :::: Oroboros Instruments | ||
:::: High- | :::: High-resolution respirometry | ||
:::: Schöpfstrasse 18 | :::: Schöpfstrasse 18 | ||
:::: A-6020 Innsbruck, Austria | :::: A-6020 Innsbruck, Austria | ||
Line 237: | Line 318: | ||
::::» '''[[IOC recommended reading]]''' | ::::» '''[[IOC recommended reading]]''' | ||
::::» '''[[MitoPedia: Respiratory states]]''' | ::::» '''[[MitoPedia: Respiratory states]]''' | ||
:::: [[Image:P.jpg|link=OXPHOS | :::: [[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] [[Image:R.jpg|link=ROUTINE respiration|ROUTINE]] [[Image:E.jpg|link=ET capacity|ETS]] [[Image:L.jpg|link=LEAK respiration|LEAK]] [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]] | ||
Line 246: | Line 327: | ||
{{Labeling | {{Labeling | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional=ORO, IOC, MitoFit, SPIN, 2018 | |additional=ORO, IOC, MitoFit, SPIN, 2018, MitoGlobal | ||
}} | }} | ||
[[Category:O2k-Workshops]] | [[Category:O2k-Workshops]] |
Latest revision as of 15:45, 8 June 2020
MiPNet23.04 IOC133 Innsbruck AT
Innsbruck AT, 2018 Sep 05-07. MitoFit Coaching Days - Basic IOC 133. |
Oroboros (2018-09-05) Mitochondr Physiol Network
Abstract:
MitoFit Coaching Days - Basic. Innsbruck, Austria; 2018 Sep 05-07.
• O2k-Network Lab: AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit
General information
- MitoFit Coaching Days - Basic provide a fundamental introduction to high-resolution respirometry with the Oroboros O2k. Hands-on sessions range from OroboPOS technical service to respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols.
Venue
- » Information on travel and venue: IOC Innsbruck.
- » Hotel suggestion: Hotel Weisses Kreuz
Registration
- closed
Tutors, lecturers, and managers
Carolina Doerrier, PhD., Oroboros Instruments - Chief scientific officer
Timea Komlòdi, PhD., Oroboros Instruments - Scientific researcher
Manuela Passrugger, BSc., Oroboros Instruments - Biomedical Assistant
Marco di Marcello, BSc., Medical University of Innsbruck, MitoFit project - Assistant
Erich Gnaiger, PhD., Oroboros Instruments, CEO
Programme
Tuesday 4th Topic Room 17:00 - 18:00 Welcome - Get-together: Introduction of participants and their research interests MiPArt
Wednesday 5th Topic Room 09:00 OroboPOS service and O2k instrumental setup - overview with videoclips ORO-MitoFit Lab 09:30 Hands-on: OroboPOS service and O2k instrumental setup ORO-MitoFit Lab 10:10 Hands-on: DatLab 7 ORO-MitoFit Lab 10:30 Coffee 11:00 Instrumental quality control 1: oxygen calibration ORO-MitoFit Lab 11:30 Hands-on: Instrumental quality control 1: oxygen calibration DL-Protocol: O2k-cleaning before use
DL-Protocol: O2 calibration air
ORO-MitoFit Lab 12:00 Lunch break 13:00 Hands-on:Instrumental quality control 1: oxygen calibration (continuation) DL-Protocol: O2 calibration air
ORO-MitoFit Lab 13:30 Instrumental quality control 2: Instrumental O2 background – overview with videoclips ORO-MitoFit Lab 14:00 Hands-on:Instrumental quality control 2: Instrumental O2 background DL-Protocol: Instrumental O2 background TiP2k
ORO-MitoFit Lab 15:00 Coffee 15:30-18:00 Hands-on: Instrumental quality control 1 and 2. DatLab analysis. ORO-MitoFit Lab
Thursday 6th Topic Room 09:00 Hands-on: Instrumental quality control 1: oxygen calibration DL-Protocol: O2k-cleaning before use
DL-Protocol: O2 calibration air
ORO-MitoFit Lab 09:15 Application of the O2k Series H ORO-MitoFit Lab 09:30 Introduction to substrate-uncoupler-inhibitor titration (SUIT) protocols – fundamental principles. SUIT reference protocol: RP1&RP2 ORO-MitoFit Lab 10:00 Coffee 10:30 Hands-on: O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2. DL-Protocol: SUIT-1_O2_pce_D03.DLP
DL-Protocol: SUIT-2 O2 pce D07.DLP
ORO-MitoFit Lab 11:45 Hands-on: O2k-cleaning after use DL-Protocol: O2k-cleaning after use
ORO-MitoFit Lab 12:00 Lunch break 13:00 Hands-on: O2k-cleaning after use DL-Protocol: O2k-cleaning after use
ORO-MitoFit Lab 13:30 Introduction to DatLab analysis ORO-MitoFit Lab 15:00 Coffee 15:30-18:00 Individual DatLab analysis + summary ORO-MitoFit Lab
Friday 7th Topic Room 09:00 MitoFit proficiency test: Hands-on: Instrumental quality control 1: oxygen calibration
DL-Protocol: O2k-cleaning before use
DL-Protocol: O2 calibration air
ORO-MitoFit Lab 10:00 Coffee 10:30 MitoFit proficiency test: Hands-on: O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2.
DL-Protocol: SUIT-1_O2_pce_D03.DLP
DL-Protocol: SUIT-2 O2 pce D07.DLP
ORO-MitoFit Lab 11:45 Hands-on: O2k-cleaning after use DL-Protocol: O2k-cleaning after use
ORO-MitoFit Lab 12:00 Lunch break 13:00 Hands-on: O2k-cleaning after use DL-Protocol: O2k-cleaning after use
ORO-MitoFit Lab 13:15 DatLab analysis ORO-MitoFit Lab 14:00 DatLab analysis summary ORO-MitoFit Lab 15:00 Coffee 15:30 The Bioblast wiki, O2k-Network and MITOEAGLE MiPArt 16:15 Feedback & conclusions MiPArt 17:00 Farewell activity MiPArt
Participants
Participant | Institution | |
---|---|---|
Bergmeister Lisa | AT_Innsbruck_Schwarzer C - Medical University, Innsbruck (AT) | |
Daltro Cardoso Luiza Helena | AT Innsbruck Oroboros - Oroboros Instruments, Innsbruck (AT) | |
Roy Chowdhury Subir | CA Winnipeg Banerji V - University of Manitoba, Winnipeg (CA) | |
Gallee Leon | AT Innsbruck Oroboros - Oroboros Instruments, Innsbruck (AT) | |
Garipi Enis | AT Innsbruck Oroboros - Oroboros Instruments, Innsbruck (AT) | |
Participant | Huete-Ortega Maria | AT Innsbruck Oroboros - Oroboros Instruments, Innsbruck (AT) |
Neufang Nora Sophie | DE Hamburg Neufang NS - Universitätsklinikum Eppendorf, Hamburg (DE) | |
Perez Valencia Juan Alberto | AT Innsbruck Oroboros - Leopold-Franzens-University, Innsbruck (AT) |
Contact
- Oroboros Instruments
- High-resolution respirometry
- Schöpfstrasse 18
- A-6020 Innsbruck, Austria
- Tel: +43 512 566796
- Fax: +43 512 566796 20
- Mitochondria and cell research
Project support
IOC recommended reading
O2k-Workshops and MitoFit Coaching Days are listed as MitoGlobal Events.
Labels:
HRR: Oxygraph-2k
ORO, IOC, MitoFit, SPIN, 2018, MitoGlobal