Difference between revisions of "MiPNet22.04 IOC120 Barcelona ES"

Latest revision as of 11:59, 27 May 2019

MiPNet22.04 IOC120 Barcelona ES

Barcelona ES, 2017 Mar 20. O2k-Workshop on high-resolution respirometry (HRR) IOC120. »ES Barcelona Zorzano A

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Oroboros (2017-03-20) Mitochondr Physiol Network

Abstract:

O2k-Workshop on high-resolution respirometry - satellite to MitoEAGLE Barcelona 2017. Barcelona, Spain; 2017 Mar 20.

• O2k-Network Lab: AT_Innsbruck_Oroboros, ES Barcelona Zorzano A

General information

O2k-Fluorometer demo experiment: high-resolution respirometry and H₂O₂ production (Amplex red)
Followed by the Barcelona MitoEAGLE Conference, MG and WC Meeting.

Programme

Monday 20th	Topic	Weblink
08:45	Registration
09:00-09:15	Welcome by Prof. Antonio Zorzano.
09:15-09:30	Introduction of participants and their research interests.	IOC120
09:30-10:00	Get started with the O2k: Overview with video clips.	O2k-Manual
10:00-11:30	Comprehensive OXPHOS analysis: substrate-uncoupler-inhibitor titration (SUIT) protocols for respiratory control by coupling and mitochondrial pathways, SUIT reference assay.	The Blue Book - SUIT reference protocol
11:30-12:00	Coffee/tea break
12:00-13:00	Principles of high-resolution respirometry: From switching on the O2k to the experimental result – oxygen sensor calibration and quality control.	POS-calibration-SOP
13:00-14:00	O2k-Fluorometry and OXPHOS analysis: Amplex red assay of H2O2 production and design of experimental protocol.	O2k-Fluorometry
14:00-15:00	Lunch
15:00-17:30	Demo-Experiment: HRR and O2k-Fluorometry with liver mitochondria – respiration and H2O2 production applying the SUIT reference assay: Protocol driver for RP1&RP2.	RP1 - RP2
17:30-18:30	Protocol driver for Marks and data analysis.
18:30-20:00	The Bioblast wiki, O2k-Network and feedback discussion.

Venue

Barcelona Science Park

Room 1 Tower D

R+D+I Towers building

C/ Baldiri Reixac 4-8

08028 Barcelona

To get to the workhsop location following routes are possible:

Start in Barcelona centrum: easiest way is to take the Metro Line 3 (L3) to Palau Reial and walk approximately 5 min to the final destination

Another option is to take a bus(see picture below with bus plan)

Start airport: take the Metro Line 9 (L9) to Zona Universitaria, which will take around 30 min

Taxi will take about 20 min and the costs are approximately 20-25 €
Another option is the train (Renfe to Sants Station) and then there the Metro L3 to Palau Reial

Registration

Please register here for the O2k-Workshop.
Registration deadline: March 13

Lecturers and tutors

List of participants

	Participant	Institution
	Barros Susana	ES_Barcelona_Zorzano A: IRB Barcelona (ES)
	Martín Cabezuelo Rubén	Universitat Politècnica de València (UPV)
	Gama Perez Pau	ES Barcelona Garcia-Roves PM : Physiological Science Department, University of Barcelona (ES)
	Garten Antje	UK_Birmingham_Lavery GG: Institute of Metabolism and Systems Research, University of Birmingham (UK)
	Giovarelli Matteo	IT_Milan_Clementi_E: Department of Biomedical and Clinical Sciences University of Milan, University of Milan (IT)
	Gonzalo Hugo	ES Lleida Boada J: IRB Lleida (ES)
	Granado Serrano Ana Belén	ES Lleida Boada J: IRB Barcelona (ES)
	Herrero Laura	Dpt. Biochemistry. School of Pharmacy. Univ. Barcelona, University of Barcelona (ES)
Javier Iglesias-Gonzalez	Iglesias-Gonzalez Javier	UK_Manchester_Galli GL: Division of Cell Matrix Biology and Regenerative Medicine, University of Manchester (UK)
	Ingram Thomas	University of Nottingham (UK)
	Koenig Magdalena	Goethe University, Frankfurt (DE)
	Lyakhovich Alexander	Research Institute VHIR, Barcelona (ES)
	Nemec Michal	Institute of Experimental Endocrinology, Bratislava (SK)
	Nogueras Lara	ES Lleida Boada J: IRB Lleida (ES)
	Ost Mario	DE Nuthetal Klaus S: Department of Pharmacology, German Institute of Human Nutrition (DE)
	Rivera-Ingraham Georgina	Université de Montpellier (FR)
	Sabate Alba	ES Barcelona Zorzano A: IRB Barcelona (ES)
	Sánchez Feutrie Manuela	ES Barcelona Zorzano A: Department of Biochemistry and Molecular Biology, University of Barceona (ES)
	Sebastián David	ES_Barcelona_Zorzano A: IRB Barcelona (ES)
	Soler Vázquez Maria Del Carmen	Department of Biochemistry and Physiology, University of Barcelona (ES)
	Thinnes Anna	Goethe University, Frankfurt (DE)
	Zagmutt Caroca Sebastián	University of Barcelona (ES)

Contact

[email protected]

Oroboros Instruments

high-resolution respirometry

Schöpfstrasse 18

A-6020 Innsbruck, Austria

Tel: +43 512 566796

Fax: +43 512 566796 20

Mitochondria and cell research

Project support

Contribution to K-Regio project MitoFit, funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.

IOC recommended reading

» IOC recommended reading

O2k-Workshops and MitoFit Coaching Days are listed as MitoGlobal Events.

Labels:

HRR: Oxygraph-2k

ORO, 2017, IOC, O2k-Network

@@ Line 2: / Line 2: @@
 {{Publication
 |title= [[Image:O2k-Network.png|right|60px|link=O2k-Network|O2k-Network]]
-'''Barcelona ES''', 2017 Mar 20. O2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry '''IOC120'''. »[[ES Barcelona Zorzano A]]
+'''Barcelona ES''', 2017 Mar 20. O2k-Workshop on high-resolution respirometry (HRR) '''IOC120'''. »[[ES Barcelona Zorzano A]]
-|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/0/0e/MiPNet22.04_IOC120_Barcelona_ES.pdf |Bioblast pdf]]
+|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/0/0e/MiPNet22.04_IOC120_Barcelona_ES.pdf|Bioblast pdf]]
-|authors=OROBOROS
+|authors=Oroboros
 |year=2017-03-20
 |journal=Mitochondr Physiol Network
 |abstract=[[Image:O2k-Workshops.png|left|130px|link=O2k-Workshops]]
-'''O2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry''' - satellite to [[MITOEAGLE Barcelona 2017]]. Barcelona, Spain; 2017 Mar 20.
+'''O2k-Workshop on high-resolution respirometry ''' - satellite to [[MitoEAGLE Barcelona 2017]]. Barcelona, Spain; 2017 Mar 20.
-|mipnetlab=AT_Innsbruck_OROBOROS, ES Barcelona Zorzano A
+|mipnetlab=AT_Innsbruck_Oroboros, ES Barcelona Zorzano A
 }}
 __TOC__
 == General information ==
 ::::* O2k-Fluorometer demo experiment: high-resolution respirometry and H<sub>2</sub>O<sub>2</sub> production (Amplex red)
-::::* Followed by the [[MITOEAGLE Barcelona 2017|Barcelona MITOEAGLE Conference, MG and WC Meeting.]]
+::::* Followed by the [[MitoEAGLE Barcelona 2017|Barcelona MitoEAGLE Conference, MG and WC Meeting.]]
 == Programme ==
@@ Line 63: / Line 64: @@
 |15:00-17:30
 |'''Demo-Experiment''': HRR and O2k-Fluorometry with liver mitochondria – respiration and H2O2 production applying the SUIT reference assay: Protocol driver for RP1&RP2.
-|[[SUIT FNSGp(PGM)01|RP1]] - [[SUIT FNSGp(PGM)02|RP2]]
+|[[SUIT-001|RP1]] - [[SUIT FNSGp(PGM)02|RP2]]
 |-
 |17:30-18:30
@@ Line 110: / Line 111: @@
 </gallery>
-== Preliminary list of participants ==
+== List of participants ==
 ::::{| class="wikitable"
@@ Line 117: / Line 118: @@
 ! Participant
 ! Institution
+|-
+|[[File:Barros Susana.jpg|right|80px|Susana Barros]]
+|[[Barros Susana]]
+|'''[[ES_Barcelona_Zorzano A]]:''' IRB Barcelona (ES)
 |-
 |
-| [[Garten A| Antje Garten]]
+| [[Martin Cabezuelo R|Martín Cabezuelo Rubén]]
-| '''[[UK_Birmingham_Manjeri_G]]:''' Institute of Metabolism and Systems Research, University of Birmingham (UK)
+| Universitat Politècnica de València (UPV)
-|-
-| [[File:Herrero_Laura.jpg|right|80px|Laura Herrero]]
-| [[Herrero L| Laura Herrero]]
-| Dpt. Biochemistry. School of Pharmacy. Univ. Barcelona, University of Barcelona (ES)
 |-
 |
-| [[Gama Perez P| Pau Gama]]
+| [[Gama Perez Pau]]
 | '''[[ES Barcelona Garcia-Roves PM ]]:''' Physiological Science Department, University of Barcelona (ES)
 |-
 |
-| [[Lyakhovich A| Alexander Lyakhovich]]
+| [[Garten Antje]]
-| Research Institute VHIR, Barcelona (ES)
+| '''[[UK_Birmingham_Lavery GG]]:''' Institute of Metabolism and Systems Research, University of Birmingham (UK)
 |-
-| [[File:Zorzano_sebastian.jpg|right|80px|David Sebastián]]
+|
-| [[Sebastian D| David Sebastián]]
+| [[Giovarelli Matteo]]
-| '''[[ES_Barcelona_Zorzano A]]:''' IRB Barcelona (ES)
+| '''[[IT_Milan_Clementi_E]]:''' Department of Biomedical and Clinical Sciences University of Milan, University of Milan (IT)
 |-
 |
-| [[Zagmutt Caroca S|Sebastián Zagmutt Caroca]]
+|[[Gonzalo Hugo]]
-| University of Barcelona (ES)
+| '''[[ES Lleida Boada J]]:''' IRB Lleida (ES)
+|-
+|
+|[[Granado Serrano Ana Belen|Granado Serrano Ana Belén]]
+|'''[[ES Lleida Boada J]]:''' IRB Barcelona (ES)
+|-
+| [[File:Herrero_Laura.jpg|right|80px|Laura Herrero]]
+| [[Herrero Laura]]
+| Dpt. Biochemistry. School of Pharmacy. Univ. Barcelona, University of Barcelona (ES)
+|-
+|[[File:Iglesias-Gonzalez Javier.JPG|right|80px|Javier Iglesias-Gonzalez]]
+|[[Iglesias-Gonzalez Javier]]
+|'''[[UK_Manchester_Galli GL]]:''' Division of Cell Matrix Biology and Regenerative Medicine, University of Manchester (UK)
+|-
+|[[File:Ingram Thomas.JPG|right|80px|Thomas Ingram]]
+|[[Ingram Thomas]]
+|University of Nottingham (UK)
+|-
+|[[File:Koenig Magdalena.jpg|right|80px|Magdalena König]]
+|[[Koenig Magdalena]]
+|Goethe University, Frankfurt (DE)
 |-
 |
-| [[Giovarelli M| Matteo Giovarelli]]
+| [[Lyakhovich Alexander]]
-| '''[[IT_Milan_Clementi_E]]:''' Department of Biomedical and Clinical Sciences University of Milan, University of Milan (IT)
+| Research Institute VHIR, Barcelona (ES)
 |-
-|
+|[[File:Nemec Michal.jpg|right|80px|Michal Nemec]]
-| [[Martin Cabezuelo R| Rubén Martín Cabezuelo]]
+|[[Nemec Michal]]
-| Universitat Politècnica de València (UPV)
+|Institute of Experimental Endocrinology, Bratislava (SK)
 |-
 |
-|[[Nogueras L| Lara Nogueras]]
+|[[Nogueras Lara]]
 | '''[[ES Lleida Boada J]]:''' IRB Lleida (ES)
 |-
-|
+|[[File:Ost M.jpg|right|80px|Mario Ost]]
-|[[Gonzalo H| Hugo Gonzalo]]
+|[[Ost Mario]]
-| '''[[ES Lleida Boada J]]:''' IRB Lleida (ES)
+|'''[[DE Nuthetal Klaus S]]:''' Department of Pharmacology, German Institute of Human Nutrition (DE)
 |-
 |
-|[[Iglesias-Gonzalez J| Javier Iglesias-Gonzalez]]
+|[[Rivera-Ingraham Georgina]]
-|'''[[UK_Manchester_Galli GL]]:''' Division of Cell Matrix Biology and Regenerative Medicine, University of Manchester (UK)
+|Université de Montpellier (FR)
 |-
 |
-|[[Sabate A|Alba Sabaté]]
+|[[Sabate Alba]]
 |'''[[ES Barcelona Zorzano A]]:''' IRB Barcelona (ES)
-|-
-|[[File:Barros Susana.jpg|right|80px|Susana Barros]]
-|[[Barros S|Susana Barros]]
-|'''[[ES_Barcelona_Zorzano A]]:''' IRB Barcelona (ES)
 |-
 |
-|[[Granado Serrano AB|Ana Belén Granado-Serrano]]
+|[[Sánchez Feutrie Manuela]]
-|'''[[ES_Barcelona_Zorzano A]]:''' IRB Barcelona (ES)
+|'''[[ES Barcelona Zorzano A]]:''' Department of Biochemistry and Molecular Biology, University of Barceona (ES)
 |-
-|[[File:Tei Bismark-Newton.jpg|right|80px|Bismark Newton Tei]]
+| [[File:Zorzano_sebastian.jpg|right|80px|David Sebastián]]
-|[[Tei BN|Bismark Newton Tei]]
+| [[Sebastián David]]
-|Department of Chemistry, University of Cape Coast (GH)
+| '''[[ES_Barcelona_Zorzano A]]:''' IRB Barcelona (ES)
 |-
-|[[File:Nemec Michal.jpg|right|80px|Michal Nemec]]
+|
-|[[Nemec M|Michal Nemec]]
+|[[Soler Vázquez Maria Del Carmen]]
-|Institute of Experimental Endocrinology, Bratislava (SK)
+|Department of Biochemistry and Physiology, University of Barcelona (ES)
 |-
-|
+|[[File:Thinnes Anna.JPG|right|80px|Anna Thinnes]]
-|[[Koenig M|Magdalena König]]
+|[[Thinnes Anna]]
 |Goethe University, Frankfurt (DE)
 |-
 |
-|[[Thinnes A|Anna Thinnes]]
+| [[Zagmutt Caroca Sebastián]]
-|Goethe University, Frankfurt (DE)
+| University of Barcelona (ES)
 |}
@@ Line 197: / Line 214: @@
-:::: OROBOROS INSTRUMENTS
+:::: Oroboros Instruments
 :::: high-resolution respirometry
 :::: Schöpfstrasse 18
@@ Line 221: / Line 238: @@
 {{Labeling
 |instruments=Oxygraph-2k
-|additional=ORO, IOC, 2017, Next, O2k-Network
+|additional=ORO, 2017, IOC, O2k-Network
 }}
 [[Category:O2k-Workshops]]