MiPNet08.12 IOC22: Difference between revisions
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[[Image:MiPNet08.12.jpg|400px|centre|thumb|Figure 1. Oxygen dependence of mt-respiration and competitive inhibition by NO. The full line shows oxygen kinetics at state 3 with pyruvate and malate in the absence of NO, measured in the physiological oxygen range (from Gnaiger et al. 1998a). Dotted lines show inhibition of respiration by the indicated NO concentrations, where measurements were performed with low-resoltion respirometry and are restricted to the high oxygen range (from Koivisto et al. 1977). Extrapolations into the physiological oxygen range (shaded region) suggest sigmoidal oxygen kinetics, which requires testing by direct measurements at low oxygen (modified after Gnaiger, Kuznetsov 2002).]] | [[Image:MiPNet08.12.jpg|400px|centre|thumb|Figure 1. Oxygen dependence of mt-respiration and competitive inhibition by NO. The full line shows oxygen kinetics at state 3 with pyruvate and malate in the absence of NO, measured in the physiological oxygen range (from Gnaiger et al. 1998a). Dotted lines show inhibition of respiration by the indicated NO concentrations, where measurements were performed with low-resoltion respirometry and are restricted to the high oxygen range (from Koivisto et al. 1977). Extrapolations into the physiological oxygen range (shaded region) suggest sigmoidal oxygen kinetics, which requires testing by direct measurements at low oxygen (modified after Gnaiger, Kuznetsov 2002).]] | ||
[http://www.bioblast.at/index.php/Aguirre_2010_Biochim_Biophys_Acta Reference: Biochim Biophys Acta 1797: 557-565 (2010) | [http://www.bioblast.at/index.php/Aguirre_2010_Biochim_Biophys_Acta Reference: Biochim Biophys Acta 1797: 557-565 (2010)] | ||
See also: [http://www.oroboros.at/index.php?id=no-oxygen Combined O2 and NO measurement and oxygen kinetics] | See also: [http://www.oroboros.at/index.php?id=no-oxygen Combined O2 and NO measurement and oxygen kinetics] | ||
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|couplingstates=OXPHOS | |couplingstates=OXPHOS | ||
|instruments=Oxygraph-2k, NO, Protocol | |instruments=Oxygraph-2k, NO, Protocol | ||
|additional=O2k-Demo}} | |additional=O2k-Demo, O2k-MultiSensor}} | ||
[[Category:OroboPedia]] | [[Category:OroboPedia]] |
Revision as of 22:07, 17 May 2014
NO effect on mitochondrial oxygen kinetics at low oxygen. Oxygraph-2k workshop Report. |
ยป Open Access-MiPNet08.12.pdf; Versions
OROBOROS (2003) Mitochondr Physiol Network
Abstract: Gnaiger E, Doeller JE, Kraus D, Shiva S, Brookes PS, Darley-Usmar VM (2003) NO effect on mitochondrial oxygen kinetics at low oxygen. Oxygraph-2k workshop Report. Mitochondr Physiol Network 08.12.
A single pilot experiment was carried out during an Oxygraph-2k workshop on high-resolution respirometry (IOC22). Respiration of isolated rat liver mitochondria was inhibited by addition of NO, which increased the sensitivity to oxygen >25-fold when compared to the half-saturation oxygen pressure, p50, in the absence of NO. Oxygen kinetics followed a monophasic hyperbolic function up to 2.2 kPa with NO (p50=0.93 kPa), compared to the standard oxygen range to 1.1 kPa without NO (p50=0.035 kPa).
Reference: Biochim Biophys Acta 1797: 557-565 (2010)
See also: Combined O2 and NO measurement and oxygen kinetics
- >> Product: OROBOROS Oxygraph-2k, O2k-Catalogue
โข O2k-Network Lab: AT_Innsbruck_OROBOROS, US_AL Birmingham_Kraus DW
Labels: MiParea: Respiration, Instruments;methods
Stress:Hypoxia, RONS; Oxidative Stress"RONS; Oxidative Stress" is not in the list (Cell death, Cryopreservation, Ischemia-reperfusion, Permeability transition, Oxidative stress;RONS, Temperature, Hypoxia, Mitochondrial disease) of allowed values for the "Stress" property. Organism: Rat Tissue;cell: Liver Preparation: Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Regulation: Inhibitor, O2"O2" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property. Coupling state: OXPHOS
HRR: Oxygraph-2k, NO, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
O2k-Demo, O2k-MultiSensor