MiPMap

high-resolution terminology - matching measurements at high-resolution

MiPMap

Description

The project Mitochondrial Physiology Map (MiPMap) is initiated to create a list of mitochondrial properties in cell types, tissues and species. As part of MitoPedia, MiPMap will provide an information synthase for Comparative Mitochondrial Physiology. This project will require global input to establish a comprehensive database.

A comparative database of mitochondrial physiology may provide the key for understanding the functional implications of mitochondrial diversity from mouse to man, and evaluation of altered mitochondrial respiratory control patterns in health and disease (Gnaiger 2009).

Abbreviation: MiPMap

Reference: Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle. New perspectives of mitochondrial physiology. Int J Biochem Cell Biol 41: 1837-1845.

MitoPedia methods: Respirometry, Fluorometry, Spectrophotometry 

MitoPedia topics: "Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property. Respiratory state"Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property., Enzyme, Media for respirometry, Inhibitor, Substrate and metabolite, Uncoupler, Permeabilization agent 

MiPMap list of mitochondrial properties in organisms (species), and cell types and tissues

1. Mammals and model organisms, Non-mammals

Work in progress

As a test case, O2k-Publications are listed according to organisms:

>> Organisms

For example, in MiPMap you would find information on mouse cell lines and tissues, e.g. mouse kidney mitochondria:

* Is malic enzyme present?

* Is the P/E<1.0 like in mouse brain but different from mouse skeletal muscle?

* Which UCPs are present?

* Preparation of isolated mitochondria, permeabilized tissue, homogenate.

* Which respiration medium is recommended? ->> Do not use media with high K⁺, which inhibits kidney mitochondrial respiration.

>> Is this a general issue for kidney, or is it in addition also a species issue? (according to Calzia E MiR05 works fine with kidney tissue from pig, but not in mouse). >> Limitations of using MiR05 to MiR06Cr

2. Tissues, cell types and cell lines

Or you may find Skeletal muscle mitochondria studied across species:

* Skeletal muscle - Human

->> O2k-Publications: Human_-_Skeletal_muscle

3. Stress conditions and pathological states

Work in progress

Medical and Pharmacological

Initiation

• Presented at Bioblast 2012.
• Focus for MiPconference on Comparative Mitochondrial Physiology.
• Link to Mitochondrial Global Network