Flux baseline correction: Difference between revisions
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ย ''J<sub>V</sub>''(bc) = ''J<sub>V</sub>'' - ''J<sub>V</sub>''<sub>0</sub> | ย ''J<sub>V</sub>''(bc) = ''J<sub>V</sub>'' - ''J<sub>V</sub>''<sub>0</sub> | ||
ย ''J<sub>V</sub>'' = (d''c''/d''t'') ยท ''ฮฝ''<sup>-1</sup> ยท ''SF'' - ''Jยฐ<sub>V</sub>'' | ย ''J<sub>V</sub>'' = (d''c''/d''t'') ยท ''ฮฝ''<sup>-1</sup> ยท ''SF'' - ''Jยฐ<sub>V</sub>'' | ||
For the oxygen channel, ''J<sub>V</sub>'' is O2 flux per volume [pmol/(sยทml)] (or volume-specific O<sub>2</sub> flux), ''c'' is the oxygen conentration [nmol/ml = ยตmol/l = ยตM], d''c''/d''t'' is the (positive) slope of oxygen concentration over time [nmol/(s ยท ml)], ''ฮฝ''<sup>-1</sup> = -1 is the stoichiometric coefficient for the reaction of oxygen consumption (oxygen is removed in the chemical reaction, thus the stoichiometric coefficient is negative, expressing oxygen flux as the negative slope), ''SF''=1,000 is the scaling factor (converting units for the amount of oxygen from nmol to pmol), and ''Jยฐ<sub>V</sub>'' is the volume-specific background oxygen flux ([[background correction]]. | For the oxygen channel, ''J<sub>V</sub>'' is O2 flux per volume [pmol/(sยทml)] (or volume-specific O<sub>2</sub> flux), ''c'' is the oxygen conentration [nmol/ml = ยตmol/l = ยตM], d''c''/d''t'' is the (positive) slope of oxygen concentration over time [nmol/(s ยท ml)], ''ฮฝ''<sup>-1</sup> = -1 is the stoichiometric coefficient for the reaction of oxygen consumption (oxygen is removed in the chemical reaction, thus the stoichiometric coefficient is negative, expressing oxygen flux as the negative slope), ''SF''=1,000 is the scaling factor (converting units for the amount of oxygen from nmol to pmol), and ''Jยฐ<sub>V</sub>'' is the volume-specific background oxygen flux ([[background correction]]). | ||
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{{MitoPedia O2k and high-resolution respirometry | {{MitoPedia O2k and high-resolution respirometry | ||
|mitopedia O2k and high-resolution respirometry=DatLab | |mitopedia O2k and high-resolution respirometry=DatLab | ||
}} | }} | ||
Revision as of 06:03, 16 March 2016
- high-resolution terminology - matching measurements at high-resolution
Flux baseline correction
Description
Flux baseline correction provides the option to display the plot and all values of the flux (or flow, or flux control ratio) as the total flux, J, minus a baseline flux, J0.
JV(bc) = JV - JV0 JV = (dc/dt) ยท ฮฝ-1 ยท SF - JยฐV
For the oxygen channel, JV is O2 flux per volume [pmol/(sยทml)] (or volume-specific O2 flux), c is the oxygen conentration [nmol/ml = ยตmol/l = ยตM], dc/dt is the (positive) slope of oxygen concentration over time [nmol/(s ยท ml)], ฮฝ-1 = -1 is the stoichiometric coefficient for the reaction of oxygen consumption (oxygen is removed in the chemical reaction, thus the stoichiometric coefficient is negative, expressing oxygen flux as the negative slope), SF=1,000 is the scaling factor (converting units for the amount of oxygen from nmol to pmol), and JยฐV is the volume-specific background oxygen flux (background correction).
Abbreviation: bc
MitoPedia O2k and high-resolution respirometry:
DatLab
