Fluorescence-Control Unit: Difference between revisions
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In this configuration the O2k can be used for high-resolution respirometry and fluorometry. It is not necessary to dismount the Fluorescence-Control Unit for basic HRR when a fluorescence singal is not recorded. | In this configuration the O2k can be used for high-resolution respirometry and fluorometry. It is not necessary to dismount the Fluorescence-Control Unit for basic HRR when a fluorescence singal is not recorded. | ||
'''Next step in setup''': [[Filter-Cap#Mounting_a_Filter-Cap|Mounting_a_Filter-Cap]] | |||
=== Current-Control === | === Current-Control === | ||
Revision as of 18:56, 11 March 2012
Fluorescence-Control Unit
| Description | Fluorescence-Control Unit with O2k-Front Fixation, Current-Control (O2k-Chamber A and B) for regulation of light intensity of the photodiodes in the fluorescence sensors. A component of the O2k-Fluorescence LED2-Module. |
|---|---|
| Product ID | 44100-01 |
| Type | O2k, MultiSensor, O2k-Fluorescence LED2-Module, O2k-Manual"O2k-Manual" is not in the list (O2k, O2k-Module, O2k-Core, O2k-Fluorometer, O2k-Main Unit, O2k-Assembly Kit, OroboPOS, OroboPOS-Service Kit, ISS, TIP2k, ...) of allowed values for the "Product type" property. |
| Link | O2k-Fluorescence LED2-Module |
| Image |
O2k-Manual
Setup of the O2k-Fluorescence LED2-Module
- Switch off the O2k with the power switch on the rear of the O2k-Main Unit.
- Remove both blue O2k-Window Frames. Insert the O2k-Window Tool around the outer rim of the window frame and unscrew counter clockwise.
- Remove the Sensor-Guide (‘nose’) from the O2k-Front Fixation of the Fluorescence-Control Unit. Loosen the small fixation screw and pull out the Sensor-Guide.
- Place the Fluorescence-Control Unit below the O2k-Chamber Block. Align the windows of the O2k-Front-Fixation with the windows of the O2k-Chamber Block, re-insert the O2k-Window Frames, and screw them finger-tight onto the O2k-Main Unit.
- Re-attach the Sensor-Guide to the O2k-Front-Fixation. Fasten the fixation screw finger-tight.
- Place the power-cables from the rear of the Fluorescence-Control Unit in the middle below the O2k-Main Unit from front to rear. Unplug the mains power cable of the O2k and plug it into the female plug of the Fluorescence-Control Unit. Insert the male plug of the Fluorescence-Control Unit into the mains socket at the rear of the O2k.
- Connect the amperometric cables attached to the side of the Fluorescence-Control Unit to the ‘Amp’ plugs (labelled "NO" in Series D-E) on the O2k-Main Unit.
In this configuration the O2k can be used for high-resolution respirometry and fluorometry. It is not necessary to dismount the Fluorescence-Control Unit for basic HRR when a fluorescence singal is not recorded.
Next step in setup: Mounting_a_Filter-Cap
Current-Control
The light intensity of the LEDs is controlled by changing the current supply independently for each fluorescence sensor (O2k-Chamber A and B).
- Position 0-8: off, 0.02, 0.5, 1, 2, 5, 10, 20, 30 mA. The polarization voltage of the Amp-Channel (NO-Channel) is set to zero in DatLab [Oxygraph]/[O2k-Control].
- Position 9: variable, controlled by the polarization voltage setting in DatLab [Oxygraph]/[O2k-Control].
