Fischer 2022 MitoFit Fe

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Fischer 2022 MitoFit Fe

Fischer C, Valente de Souza L, Komlódi T, Garcia-Souza LF, Volani C, Tymoszuk P, Demetz E, Seifert M, Auer K, Hilbe R, Brigo N, Petzer V, Asshoff M, Gnaiger E, Weiss G (2022) Mitochondrial respiration in response to iron deficiency anemia. Comparison of peripheral blood mononuclear cells and liver. MitoFit Preprints 2022.2. https://doi.org/10.26124/mitofit:2022-0002 - 2022-03-21 Published in »Metabolites«

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Mitochondrial respiration in response to iron deficiency anemia. Comparison of peripheral blood mononuclear cells and liver

MitoFit Preprints (2022-03-04) MitoFit Prep

Abstract: Journal publication 2022-03-21 in »Metabolites«

Iron is an essential component for metabolic processes including oxygen transport within hemoglobin, tricarboxylic acid (TCA) cycle activity and mitochondrial energy transformation. Iron deficiency can thus lead to metabolic dysfunction and eventually result in iron deficiency anemia (IDA) which affects approximately 1.5 billion people worldwide. Using a rat model of IDA induced by phlebotomy, we studied the effects of IDA on mitochondrial respiration in peripheral blood mononuclear cells (PBMCs) and liver. Furthermore, we evaluated whether mitochondrial function evaluated by high-resolution respirometry in PBMCs reflects corresponding alterations in the liver. Surprisingly, mitochondrial respiratory capacity was increased in PBMCs from rats with IDA compared to controls. In contrast, mitochondrial respiration remained unaffected in livers from IDA rats. Of note, citrate synthase activity indicated an increased mitochondrial density in PBMCs, whereas it remained unchanged in the liver, partly explaining the different responses of mitochondrial respiration in PBMCs and liver. Taken together, these results indicate that mitochondrial function determined in PBMCs cannot serve as a valid surrogate for respiration in the liver. Metabolic adaptions to iron deficiency resulted in different metabolic reprogramming in the blood cells and liver tissue.

• Keywords: anemia, iron deficiency, peripheral blood mononuclear cells, liver, mitochondrial function, OXPHOS, mitochondrial respiration, surrogate • Bioblast editor: Doerrier C • O2k-Network Lab: AT Innsbruck Oroboros

ORCID: Fischer Christine, Komlódi Timea, Garcia-Souza Luiz F, Volani Chiara, Tymoszuk Piotr, Demetz Egon, Gnaiger Erich, Weiss Guenter

Data availability

Original files not provided by the authors.