Coenzyme Q: Difference between revisions
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== Application in [[HRR]] == | |||
:::: '''Q2 : Coenzmye Q2''' (Sigma Aldrich,C8081, MW: 318.41; 2,3-Dimethoxy-5-methyl-6-geranyl-1,4-benzoquinone, Ubiquinone-2, oxidized form) | |||
:::: <span style="color:#8B008B"> '''Caution:''' Light sensitive (store solution in a dark glass vial).</span>. | |||
::: '''Preparation of 10 mM stock solution''' (dissolved in ethanol) (to run cyclic voltammetry) | |||
::::# Dissolve one commercial vial of Q2 (2 mg) Q2 in 628 µL 99.9% ethanol. | |||
::::# Divide into 50 µL aliquots in dark glass vials. | |||
::::# Store frozen at -20 °C. | |||
::: '''Preparation of 1 mM stock solution''' (to measure the Q redox ratio) | |||
::::# Dilute 50 µL of the 10 mM stock with 450 µL 99.9% ethanol. | |||
::::# Divide into 50 µL aliquots in dark glass vials. | |||
::::# Store frozen at -20 °C. | |||
::: '''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | |||
:::: '''Q redox ratio''' | |||
::::* Titration volume: 2 µL of the 1 mM stock solution using a 10 µL syringe (2 mL O2k-Chamber). | |||
::::* Final concentration: 1 µM | |||
:::: '''Cyclic voltammetry''' | |||
::::* Titration volume: 6 µL of the 10 mM stock solution using a 10 µL syringe (2 mL O2k-Chamber). | |||
::::* Final concentration: 30 µM | |||
{{MitoPedia concepts}} | {{MitoPedia concepts}} | ||
{{MitoPedia methods}} | {{MitoPedia methods}} | ||
Revision as of 11:19, 3 September 2019
- high-resolution terminology - matching measurements at high-resolution
Coenzyme Q
Description
Coenzyme Q, redox system (oxidized ubiquinone, partially reduced semiquinone, fully reduced ubiquinol) of the ET-pathway.
More details » Q-junction.
Abbreviation: Q, CoQ
Reference: Gnaiger 2019 MitoPathways
Application in HRR
- Q2 : Coenzmye Q2 (Sigma Aldrich,C8081, MW: 318.41; 2,3-Dimethoxy-5-methyl-6-geranyl-1,4-benzoquinone, Ubiquinone-2, oxidized form)
- Caution: Light sensitive (store solution in a dark glass vial)..
- Preparation of 10 mM stock solution (dissolved in ethanol) (to run cyclic voltammetry)
- Dissolve one commercial vial of Q2 (2 mg) Q2 in 628 µL 99.9% ethanol.
- Divide into 50 µL aliquots in dark glass vials.
- Store frozen at -20 °C.
- Preparation of 1 mM stock solution (to measure the Q redox ratio)
- Dilute 50 µL of the 10 mM stock with 450 µL 99.9% ethanol.
- Divide into 50 µL aliquots in dark glass vials.
- Store frozen at -20 °C.
- O2k manual titrations MiPNet09.12 O2k-Titrations
- Q redox ratio
- Titration volume: 2 µL of the 1 mM stock solution using a 10 µL syringe (2 mL O2k-Chamber).
- Final concentration: 1 µM
- Cyclic voltammetry
- Titration volume: 6 µL of the 10 mM stock solution using a 10 µL syringe (2 mL O2k-Chamber).
- Final concentration: 30 µM
MitoPedia topics: Enzyme, Substrate and metabolite
