Citrate synthase: Difference between revisions

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Citrate synthase

Description

Condensation of oxaloacetate with acetyl-CoA yields citrate as an entry into the TCA cycle. CS is located in the mt-matrix. CS activity is frequently used as a functional marker of the amount of mitochondria (mitochondrial elementary marker, mtE) for normalization of respiratory flux.

Abbreviation: CS

Reference: MiPNet17.04 CitrateSynthase

Normalization of respiratory flux for CS as a mitochondrial marker

Communicated by Gnaiger Erich last update 2020-04-19

Respiration of living or permeabilized cells, tissue homogenate or permeabilized tissue, and isolated mitochondria can be normalized for CS activity. Then marker-specific oxygen flux, J_O₂/CS [pmol∙s^-1∙IU^-1], is a quantitative measure of respiration that reflects mitochondrial quality, independent of mitochondrial density.

Several publications in peer-reviewed journals report respiration normalized for CS activity in units which do not make sense, e.g. [pmol∙s^-1∙mL^-1∙IU^-1], such that those numerical results are pointless. Clarification is obtained by strict definitions of normalization of rates [1] and dimensional analysis, summarized in MiPNet 17.04 [2].

Gnaiger E, Aasander Frostner E, Abdul Karim N, Abdel-Rahman EA, Abumrad NA, Acuna-Castroviejo D, Adiele RC, et al (2019) Mitochondrial respiratory states and rates. MitoFit Preprint Arch doi:10.26124/mitofit:190001.v6. - »Bioblast link«
Eigentler A, Draxl A, Gnaiger E (2020) Laboratory protocol: Citrate synthase a mitochondrial marker enzyme. Mitochondr Physiol Network 17.04(04):1-11. - »Bioblast link«

MitoPedia topics: Enzyme

@@ Line 1: / Line 1: @@
 {{MitoPedia
 |abbr=CS
-|description=Condensation of [[oxaloacetate]] with acetyl-CoA yields citrate as an entry into the [[TCA cycle]], with CS located in the mt-matrix. CS activity is frequently used as a mitochondrial marker for normalization of respiratory flux per mitochondrial elementary marker, ''mtE'', refering to CS activity as a mitochonddrial elementary unit [mtEU].
+|description=Condensation of [[oxaloacetate]] with acetyl-CoA yields citrate as an entry into the [[TCA cycle]]. CS is located in the mt-matrix. CS activity is frequently used as a functional marker of the amount of mitochondria (mitochondrial elementary marker, ''mtE'') for normalization of respiratory flux.
 |info=[[MiPNet17.04 CitrateSynthase]]
 }}
+== Normalization of respiratory flux for CS as a mitochondrial marker ==
+ Communicated by [[Gnaiger Erich]] last update 2020-04-19
+:::: Respiration of living or permeabilized cells, tissue homogenate or permeabilized tissue, and isolated mitochondria can be normalized for CS activity. Then marker-specific oxygen flux, ''J''<sub>O<sub>2</sub>/CS</sub> [pmol∙s<sup>-1</sup>∙IU<sup>-1</sup>], is a quantitative measure of respiration that reflects mitochondrial quality, independent of mitochondrial density.
+:::: Several publications in peer-reviewed journals report respiration normalized for CS activity in units which do not make sense, ''e.g.'' [pmol∙s<sup>-1</sup>∙mL<sup>-1</sup>∙IU<sup>-1</sup>], such that those numerical results are pointless. Clarification is obtained by strict definitions of normalization of rates [1] and dimensional analysis, summarized in MiPNet 17.04 [2].
+::::# Gnaiger E, Aasander Frostner E, Abdul Karim N, Abdel-Rahman EA, Abumrad NA, Acuna-Castroviejo D, Adiele RC, et al (2019) Mitochondrial respiratory states and rates. MitoFit Preprint Arch doi:10.26124/mitofit:190001.v6. - [[Gnaiger 2019 MitoFit Preprint Arch |»Bioblast link«]]
+::::# Eigentler A, Draxl A, Gnaiger E (2020) Laboratory protocol: Citrate synthase a mitochondrial marker enzyme. Mitochondr Physiol Network 17.04(04):1-11. - [[MiPNet17.04 CitrateSynthase |»Bioblast link«]]
 {{MitoPedia topics
 |mitopedia topic=Enzyme
 }}