Difference between revisions of "Citrate synthase"
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::::# Eigentler A, Draxl A, Gnaiger E (2020) Laboratory protocol: Citrate synthase a mitochondrial marker enzyme. Mitochondr Physiol Network 17.04(04):1-12. - [[MiPNet17.04 CitrateSynthase |Ā»Bioblast linkĀ«]] | ::::# Eigentler A, Draxl A, Gnaiger E (2020) Laboratory protocol: Citrate synthase a mitochondrial marker enzyme. Mitochondr Physiol Network 17.04(04):1-12. - [[MiPNet17.04 CitrateSynthase |Ā»Bioblast linkĀ«]] | ||
== References == | |||
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Revision as of 08:56, 23 May 2020
Description
Condensation of oxaloacetate with acetyl-CoA yields citrate as an entry into the TCA cycle. CS is located in the mt-matrix. CS activity is frequently used as a functional marker of the amount of mitochondria (mitochondrial elementary marker, mtE) for normalization of respiratory flux.
Abbreviation: CS
Reference: MiPNet17.04 CitrateSynthase
Normalization of respiratory flux for CS as a mitochondrial marker
Communicated by Gnaiger Erich last update 2020-04-19
- Respiration of living or permeabilized cells, tissue homogenate or permeabilized tissue, and isolated mitochondria can be normalized for CS activity. Then marker-specific oxygen flux, JO2/CS [pmolāsĀ-1āIUĀ-1], is a quantitative measure of respiration that reflects mitochondrial quality, independent of mitochondrial density.
- Several publications in peer-reviewed journals report respiration normalized for CS activity in units which do not make sense, e.g. [pmolās-Ā1āmLĀ-1āIUĀ-1], such that those numerical results are pointless. Clarification is obtained by strict definitions of normalization of rates [1] and dimensional analysis, summarized in MiPNet 17.04 [2].
- Gnaiger Erich et al ā MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. doi:10.26124/bec:2020-0001.v1. - Ā»Bioblast linkĀ«
- Eigentler A, Draxl A, Gnaiger E (2020) Laboratory protocol: Citrate synthase a mitochondrial marker enzyme. Mitochondr Physiol Network 17.04(04):1-12. - Ā»Bioblast linkĀ«
References
Bioblast link | Reference | Year |
---|---|---|
Gnaiger 2020 BEC MitoPathways | Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002 | 2020 |
BEC 2020.1 doi10.26124bec2020-0001.v1 | Gnaiger E et al ā MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v1 | 2020 |
MiPNet17.04 CitrateSynthase | Laboratory protocol: Citrate synthase a mitochondrial marker enzyme. | 2020-04-18 |
- Bioblast links: Normalization - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Rate
- Ā» Normalization of rate
- Ā» Flow
- Ā» Oxygen flow
- Ā» Flux
- Ā» Oxygen flux
- Ā» Flux control ratio
- Ā» Coupling-control ratio
- Ā» Pathway control ratio
- Ā» Flux control efficiency
- Rate
- Quantities for normalization
- Ā» Count in contrast to Number
- Ā» Mitochondrial marker
- Ā» O2k-Protocols: mitochondrial and marker-enzymes
- Ā» Citrate synthase activity
- Quantities for normalization
- General
- Ā» Extensive quantity
- Ā» Specific quantity
- Ā» Advancement
- Ā» Motive unit
- Ā» Iconic symbols
- General
- Related keyword lists
MitoPedia topics: Enzyme