Difference between revisions of "Bioblast"

Revision as of 17:40, 7 August 2013

Bioblast alert 2013(02)

MitoPedia news: The uncoupler CCCP - Carbonyl cyanide m-chloro phenyl hydrazone: In our on-going experiments with various sources of mitochondria and different mt-preparations, we observed that results on respiration and mt-membrane potential were fully comparable using CCCP or FCCP as uncouplers (protonophores) in parallel experiments. Therefore, from now on our lab will replace FCCP by CCCP. Optimum concentrations for the estimation of ETS capacity are slightly higher for CCCP compared to FCCP. Importantly, CCCP is far less expensive than FCCP (protocol development supported by MitoCom).
Selection of appropriate methodologies: A recently published review by Perry and colleagues provides a critical assessment of currently used methodologies and instruments used in respirometry – excellent reading. Perry CG, Kane DA, Lanza IR, Neufer PD (2013) Methods for assessing mitochondrial function in diabetes. Diabetes 62: 1041-1053.
MitoPedia Glossary - new section: Respiratory control ratios.

 Bioblast alert: for information purposes, not intended to be an advertisement. Contributions are welcome.

Power-O2k

Multiple O2k-units are offered as a ‘Power-O2k System’ of high-resolution respirometry for high-throughput with high output. Many MiPNet Laboratories have increased their high-resolution throughput by multiple O2k-units: Power-O2k

 The Bioblast Wiki start page has been accessed >180,000 times (2013-08-06) - What is BIOBLAST?

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-=== [[Bioblast alert|Bioblast alert]] 2013(01) ===
+=== [[Bioblast alert|Bioblast alert]] 2013(02) ===
-* MitoPedia glossary: '''[[Basal respiration]]''' in physiology, cellular bioenergetics and mitochondrial physiology - an updated article, introducing '''[[Bioblast-MitoPedia]]'''.
+* '''MitoPedia news''': The [[uncoupler]] '''[[CCCP]]''' - Carbonyl cyanide m-chloro phenyl hydrazone: In our on-going experiments with various sources of mitochondria and different mt-preparations, we observed that results on respiration and mt-membrane potential were fully comparable using CCCP or [[FCCP]] as uncouplers (protonophores) in parallel experiments. Therefore, from now on our lab will replace FCCP by CCCP. Optimum concentrations for the estimation of [[ETS capacity]] are slightly higher for CCCP compared to FCCP. Importantly, '''CCCP is far less expensive''' than FCCP (protocol development supported by ''[[MitoCom_Diagnostic_Concept|MitoCom]]'').
-* MitoGlobal project: The '''[[Mitochondrial Global Network]] (MitoGlobal)''' initiates a joint publication to review the state-of-the-art terminology in the rapidly expanding field of mitochondrial respiratory physiology. From the [[MitoPedia Glossary: Respiratory states|present start]] there is a long way to go, and we invite experts in the field to a joint walk, asking for your suggestions - [mailto:[email protected] Email to Erich Gnaiger].
+* '''Selection of appropriate methodologies''': A recently published review by Perry and colleagues provides a critical assessment of currently used methodologies and instruments used in respirometry – excellent reading. [[Perry_2013_Diabetes|Perry CG, Kane DA, Lanza IR, Neufer PD (2013) Methods for assessing mitochondrial function in diabetes. Diabetes 62: 1041-1053.]]
-* '''MiP''summer'' School 2013''': [http://www.mitophysiology.org/?MiPschool2013 Mitochondrial Physiology - "Theory & Praxis"] - the annual highlight that students of cellular bioenergetics and mitochondrial physiology cannot afford to miss! August 26-30, 2013, Copenhagen, Denmark.
+* '''MitoPedia Glossary''' - ''new section'': [[MitoPedia Glossary: Respiratory control ratios|Respiratory control ratios]].
-* '''MiP''conference'' 2013 - Comparative Mitochondrial Physiology''' [http://www.mitophysiology.org/?MiP2013 MiP2013], September 23-27, 2013, Obergurgl, Austria. ''Due to some cancellations, a limited number of late registrations can still be accepted.'' >>[[MiP2013|MiP2013 abstracts online]]