SUIT-001 O2 mt D001: Difference between revisions

Revision as of 09:32, 28 August 2017

high-resolution terminology - matching measurements at high-resolution

SUIT-001 O2 mt D001

Description

Abbreviation: FNSGp(PGM)

Reference: A: SUIT_RP1 - MiPNet21.06 SUIT RP

MitoPedia concepts: SUIT protocol, SUIT A

MitoPedia: SUIT - SUIT reference protocol RP1

SUIT-category: FNSGp(PGM)

SUIT protocol pattern: orthogonal

Harmonized SUIT protocols

SUIT RP1 SUIT RP2

SUIT RP1:1PM;2D:2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10Tm;11Azd

SUIT RP2:1D;1M.1;2Oct;2c;2M2;3P;4G;5S;6Gp;7U;8Rot;9Ama;10Tm;11Azd

Harmonization between RP1 and RP2

SUIT O2 DLP for RP1 (DatLab 7.1)

DL-Protocols (DLP) for substrate-uncoupler-inhibitor titrations (see MitoPedia: SUIT) guide through a sequence of coupling and pathway control states. A specific SUIT protocol can be assigned to O2k-Chamber A or B or both.

DL-Protocol

Sample

DLP File

O₂

O₂&Amp (H₂O₂ production)

DL-Protocol	Description	Link/Info	Download - Analysis .XLSX
RP1	SUIT reference protocol RP1 for permeabilized cells (pce) with digitonin	1PM;2D;3U;4G;5S;6Oct;7Rot;8Gp-
RP1	SUIT reference protocol RP1 for mt-preparations	1PM;2D;3U;4G;5S;6Oct;7Rot;8Gp-
RP1	SUIT reference protocol RP1 for permeabilized fibers	1PM;2D;3U;4G;5S;6Oct;7Rot;8Gp-
RP1 blood	SUIT reference protocol RP1 for blood samples	1ce;1Dig;1PM;2D;2c;3U;4G;5S;6Rot;7Gp;8Ama;9AsTm;10Azd	References

@@ Line 39: / Line 39: @@
 | style="text-align:center" |'''O<sub>2</sub>&Amp (H<sub>2</sub>O<sub>2</sub> production)'''
-|-
-| RP1
-| mt-preparations
-| ''In progress''
-| ''In progress''
-|-
-| RP1
-| Permeabiliced cells (pce) with digitonin
-| [[Media:RP1pce(Dig).DLP|RP1pce(Dig)]]
-| ''In progress''
-|-
-| RP1
-| Permeabilized cells (pce) without digitonin
-| ''In progress''
-| ''In progress''
-|-
-| RP1
-| Permeabilized fibers
-| ''In progress''
-| ''In progress''
-|-
-|}
-A full list of DL-Protocols (Instrumental DL-Protocols and SUIT DL-Protocols) is displayed  in [[DL-Protocols]].
-== DatLab-Analysis templates for RP1 (DatLab 7.1) ==
 {| class="wikitable" border="1"
 |-
-! SUIT Reference protocol
+! DL-Protocol
-! Sample
+! Description
-! O<sub>2</sub>
+! Download - Protocol .DLP
+! Link/Info
+! Download - Analysis .XLSX
 |-
 | RP1
-| mt-preparations
+| SUIT reference protocol RP1 for permeabilized cells (pce) with digitonin
-| [[Media:RP1mt.xlsx |»SUIT RP1mt]]
+| style="text-align:center"| [[File:Download1.png|20px|link=http://wiki.oroboros.at/images/0/05/RP1pce%28Dig%29.DLP]]
+| [[1PM;2D;3U;4G;5S;6Oct;7Rot;8Gp-|1PM;2D;3U;4G;5S;6Oct;7Rot;8Gp-]]
+| style="text-align:center"| [[File:Download2.png|20px|link=http://wiki.oroboros.at/images/0/02/RP1pce%28Dig%29.xlsx]]
 |-
 | RP1
-| Permeabilized cells (pce) with digitonin
+| SUIT reference protocol RP1 for mt-preparations
-| [[Media:RP1pce(Dig).xlsx |»SUIT RP1pce(Dig)]]
+| style="text-align:center"| [[File:Download1.png|20px|link=http://wiki.oroboros.at/images/a/a5/RP1mt.DLP]]
+| [[1PM;2D;3U;4G;5S;6Oct;7Rot;8Gp-#|1PM;2D;3U;4G;5S;6Oct;7Rot;8Gp-]]
+| style="text-align:center"| [[File:Download2.png|20px|link=http://wiki.oroboros.at/images/6/62/RP1mt.xlsx]]
 |-
 | RP1
-| Permeabilized cells (pce) without digitonin
+| SUIT reference protocol RP1 for permeabilized fibers
-| [[Media:RP1pce.xlsx |»SUIT RP1pce]]
+| style="text-align:center"| [[File:Download1.png|20px|link=http://wiki.oroboros.at/images/b/b9/RP1pfi.DLP]]
+| [[1PM;2D;3U;4G;5S;6Oct;7Rot;8Gp-#|1PM;2D;3U;4G;5S;6Oct;7Rot;8Gp-]]
+| style="text-align:center"| [[File:Download2.png|20px|link=http://wiki.oroboros.at/images/1/18/RP1pfi.xlsx]]
 |-
-| RP1
+| RP1 blood
-| Permeabilized fibers
+| SUIT reference protocol RP1 for blood samples
-| [[Media:RP1pfi.xlsx |»SUIT RP1pfi]]
+| style="text-align:center"| [[File:Download1.png|20px|link=http://wiki.oroboros.at/images/6/67/RP1pce%28Dig%29_blood.DLP]]
-|}
+| 1ce;1Dig;1PM;2D;2c;3U;4G;5S;6Rot;7Gp;8Ama;9AsTm;10Azd
+| style="text-align:center"| [[File:Download2.png|20px|link=http://wiki.oroboros.at/images/e/ec/RP1pce%28Dig%29_blood.xlsx]]

SUIT-001 O2 mt D001: Difference between revisions

Revision as of 09:32, 28 August 2017

Description

Harmonized SUIT protocols

SUIT O2 DLP for RP1 (DatLab 7.1)

References