Rodriguez-Juarez 2007 Biochem J: Difference between revisions
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{{Publication | {{Publication | ||
|title=Rodriguez-Juarez F, Aguirre E, Cadenas S (2007) Relative sensitivity of soluble guanylate cyclase and mitochondrial respiration to endogenous nitric oxide at physiological oxygen concentration. Biochem J 405: 223- | |title=Rodriguez-Juarez F, Aguirre E, Cadenas S (2007) Relative sensitivity of soluble guanylate cyclase and mitochondrial respiration to endogenous nitric oxide at physiological oxygen concentration. Biochem J 405:223-31. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/17441787 PMID: 17441787] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/17441787 PMID: 17441787] | ||
|authors=Rodriguez-Juarez F, Aguirre E, Cadenas S | |authors=Rodriguez-Juarez F, Aguirre E, Cadenas S | ||
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|model cell lines=Fibroblast | |model cell lines=Fibroblast | ||
|preparations=Intact cells | |preparations=Intact cells | ||
|injuries=RONS | |injuries=Oxidative stress;RONS | ||
|topics=Inhibitor, O2 | |topics=Inhibitor, O2 | ||
|couplingstates=ROUTINE | |couplingstates=ROUTINE |
Revision as of 16:47, 23 February 2015
Rodriguez-Juarez F, Aguirre E, Cadenas S (2007) Relative sensitivity of soluble guanylate cyclase and mitochondrial respiration to endogenous nitric oxide at physiological oxygen concentration. Biochem J 405:223-31. |
Rodriguez-Juarez F, Aguirre E, Cadenas S (2007) Biochem J
Abstract: Disposition of the second messenger nitric oxide (NO) in mammalian tissues occurs through multiple pathways including dioxygenation by erythrocyte hemoglobin and red muscle myoglobin. Metabolism by a putative NO dioxygenase activity in non-striated tissues has also been postulated, but the exact nature of this activity is unknown. In the present study, we tested the hypothesis that cytoglobin, a newly discovered hexacoordinated globin, participates in cell-mediated NO consumption. Stable expression of small hairpin RNA targeting cytoglobin in fibroblasts resulted in decreased NO consumption and intracellular nitrate production. These cells were more sensitive to NO-induced inhibition of cell respiration and proliferation, which could be restored by re-expression of human cytoglobin. We also demonstrated cytoglobin expression in adventitial fibroblasts as well as vascular smooth muscle cells from various species including human and found that cytoglobin was expressed in the adventitia and media of intact rat aorta. These results indicate that cytoglobin contributes to cell-mediated NO dioxygenation and represents an important NO sink in the vascular wall.
โข O2k-Network Lab: ES Madrid Cadenas S
Labels: MiParea: Respiration
Stress:Oxidative stress;RONS Organism: Rat
Preparation: Intact cells
Regulation: Inhibitor, O2"O2" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property. Coupling state: ROUTINE
HRR: Oxygraph-2k