Gautam 2023 Neurobiol Dis: Difference between revisions
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{{Publication | {{Publication | ||
|title=Gautam M, Genç B, Helmold B, Ahrens A, Kuka J, Makrecka-Kuka M, Günay A, Koçak N, Aguilar-Wickings IR, Keefe D, Zheng G, Swaminathan S, Redmon M, Zariwala HA, Özdinler PH (2023) SBT-272 improves TDP-43 pathology in ALS upper motor neurons by modulating mitochondrial integrity, motility, and function. https://doi.org/10.1016/j.nbd.2023.106022 | |title=Gautam M, Genç B, Helmold B, Ahrens A, Kuka J, Makrecka-Kuka M, Günay A, Koçak N, Aguilar-Wickings IR, Keefe D, Zheng G, Swaminathan S, Redmon M, Zariwala HA, Özdinler PH (2023) SBT-272 improves TDP-43 pathology in ALS upper motor neurons by modulating mitochondrial integrity, motility, and function. https://doi.org/10.1016/j.nbd.2023.106022 | ||
|info=Neurobiol Dis | |info=Neurobiol Dis 178:106022. [https://pubmed.ncbi.nlm.nih.gov/36716828 PMID: 36716828 Open Access] | ||
|authors=Gautam | |authors=Gautam Mukesh, Genc Baris, Helmold Benjamin, Ahrens Angela, Kuka Janis, Makrecka-Kuka Marina, Guenay Aksu, Kocak Nuran, Aguilar-Wickings Izaak R, Keefe Dennis, Zheng Guozhu, Swaminathan Suchitra, Redmon Martin, Zariwala Hatim A, Oezdinler P Hande | ||
|year=2023 | |year=2023 | ||
|journal=Neurobiol Dis | |journal=Neurobiol Dis | ||
|abstract=Mitochondrial defects are one of the common underlying causes of neuronal vulnerability in motor neuron diseases, such as amyotrophic lateral sclerosis (ALS), and TDP-43 pathology is the most common proteinopathy in ALS. Disrupted inner mitochondrial membrane (IMM) reported in the upper motor neurons (UMNs) of ALS patients with TDP-43 pathology is recapitulated in the UMNs of well-characterized mutant hTDP-43 mouse model of ALS. The construct validity, such as common cellular pathology in mice and human, offers a unique opportunity to test treatment strategies that may translate. SBT-272 is a well-tolerated brain-penetrant small molecule that stabilizes cardiolipin, a phospholipid found in IMM, thereby restoring mitochondrial structure and respiratory function. We investigated whether SBT-272 can improve IMM structure and health in UMNs diseased with TDP-43 pathology in our well-characterized UMN reporter line for ALS. We found that SBT-272 significantly improved mitochondrial structural integrity and restored mitochondrial motility and function. This led to improved health of diseased UMNs in vitro. In comparison to edaravone and AMX0035, SBT-272 appeared more effective in restoring health of diseased UMNs. Chronic treatment of SBT-272 for sixty days starting at an early symptomatic stage of the disease in vivo led to a significant reduction in astrogliosis, microgliosis, and TDP-43 pathology in the ALS motor cortex. Our results underscore the therapeutic potential of SBT-272, especially within the context of TDP-43 pathology and mitochondrial dysfunction. | |abstract=Mitochondrial defects are one of the common underlying causes of neuronal vulnerability in motor neuron diseases, such as amyotrophic lateral sclerosis (ALS), and TDP-43 pathology is the most common proteinopathy in ALS. Disrupted inner mitochondrial membrane (IMM) reported in the upper motor neurons (UMNs) of ALS patients with TDP-43 pathology is recapitulated in the UMNs of well-characterized mutant hTDP-43 mouse model of ALS. The construct validity, such as common cellular pathology in mice and human, offers a unique opportunity to test treatment strategies that may translate. SBT-272 is a well-tolerated brain-penetrant small molecule that stabilizes cardiolipin, a phospholipid found in IMM, thereby restoring mitochondrial structure and respiratory function. We investigated whether SBT-272 can improve IMM structure and health in UMNs diseased with TDP-43 pathology in our well-characterized UMN reporter line for ALS. We found that SBT-272 significantly improved mitochondrial structural integrity and restored mitochondrial motility and function. This led to improved health of diseased UMNs ''in vitro''. In comparison to edaravone and AMX0035, SBT-272 appeared more effective in restoring health of diseased UMNs. Chronic treatment of SBT-272 for sixty days starting at an early symptomatic stage of the disease ''in vivo'' led to a significant reduction in astrogliosis, microgliosis, and TDP-43 pathology in the ALS motor cortex. Our results underscore the therapeutic potential of SBT-272, especially within the context of TDP-43 pathology and mitochondrial dysfunction. | ||
|keywords=ATP, Cardiolipin, Electron transport chain, Motor neuron disease, Proteinopathy, SBT-272, TDP-43 | |||
|editor=[[Plangger M]] | |editor=[[Plangger M]] | ||
}} | }} | ||
{{Template:MitoEAGLE terminology}} | |||
{{Labeling | {{Labeling | ||
|area=Respiration | |area=Respiration, Pharmacology;toxicology | ||
|instruments=Oxygraph-2k | |diseases=Neurodegenerative | ||
|additional=2023-01 | |organism=Rat | ||
|tissues=Heart, Nervous system | |||
|preparations=Permeabilized tissue, Homogenate | |||
|couplingstates=LEAK, OXPHOS | |||
|pathways=N, S, CIV, NS, ROX | |||
|instruments=Oxygraph-2k, O2k-Fluorometer | |||
|additional=2023-01, AmR | |||
}} | }} |
Latest revision as of 12:28, 27 March 2023
Gautam M, Genç B, Helmold B, Ahrens A, Kuka J, Makrecka-Kuka M, Günay A, Koçak N, Aguilar-Wickings IR, Keefe D, Zheng G, Swaminathan S, Redmon M, Zariwala HA, Özdinler PH (2023) SBT-272 improves TDP-43 pathology in ALS upper motor neurons by modulating mitochondrial integrity, motility, and function. https://doi.org/10.1016/j.nbd.2023.106022 |
» Neurobiol Dis 178:106022. PMID: 36716828 Open Access
Gautam Mukesh, Genc Baris, Helmold Benjamin, Ahrens Angela, Kuka Janis, Makrecka-Kuka Marina, Guenay Aksu, Kocak Nuran, Aguilar-Wickings Izaak R, Keefe Dennis, Zheng Guozhu, Swaminathan Suchitra, Redmon Martin, Zariwala Hatim A, Oezdinler P Hande (2023) Neurobiol Dis
Abstract: Mitochondrial defects are one of the common underlying causes of neuronal vulnerability in motor neuron diseases, such as amyotrophic lateral sclerosis (ALS), and TDP-43 pathology is the most common proteinopathy in ALS. Disrupted inner mitochondrial membrane (IMM) reported in the upper motor neurons (UMNs) of ALS patients with TDP-43 pathology is recapitulated in the UMNs of well-characterized mutant hTDP-43 mouse model of ALS. The construct validity, such as common cellular pathology in mice and human, offers a unique opportunity to test treatment strategies that may translate. SBT-272 is a well-tolerated brain-penetrant small molecule that stabilizes cardiolipin, a phospholipid found in IMM, thereby restoring mitochondrial structure and respiratory function. We investigated whether SBT-272 can improve IMM structure and health in UMNs diseased with TDP-43 pathology in our well-characterized UMN reporter line for ALS. We found that SBT-272 significantly improved mitochondrial structural integrity and restored mitochondrial motility and function. This led to improved health of diseased UMNs in vitro. In comparison to edaravone and AMX0035, SBT-272 appeared more effective in restoring health of diseased UMNs. Chronic treatment of SBT-272 for sixty days starting at an early symptomatic stage of the disease in vivo led to a significant reduction in astrogliosis, microgliosis, and TDP-43 pathology in the ALS motor cortex. Our results underscore the therapeutic potential of SBT-272, especially within the context of TDP-43 pathology and mitochondrial dysfunction. • Keywords: ATP, Cardiolipin, Electron transport chain, Motor neuron disease, Proteinopathy, SBT-272, TDP-43 • Bioblast editor: Plangger M
On terminology
- For harmonization of terminology on respiratory states and rates, see
- Gnaiger E et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v1
- For harmonization of terminology on respiratory states and rates, see
Labels:
MitoEAGLE terminology
Labels: MiParea: Respiration, Pharmacology;toxicology
Pathology: Neurodegenerative
Organism: Rat Tissue;cell: Heart, Nervous system Preparation: Permeabilized tissue, Homogenate
Coupling state: LEAK, OXPHOS
Pathway: N, S, CIV, NS, ROX
HRR: Oxygraph-2k, O2k-Fluorometer
2023-01, AmR