Komlodi 2022 Abstract Bioblast: Difference between revisions
No edit summary |
No edit summary |
||
Line 1: | Line 1: | ||
[[File:Bioblast2022 banner.jpg|link=Bioblast_2022]] | [[File:Bioblast2022 banner.jpg|link=Bioblast_2022]] | ||
{{Abstract | {{Abstract | ||
|title=[[File:KomlodiT.JPG|left|100px|Komlodi Timea]] <u>Komlรณdi Timea</u>, Tretter L (2022) The protonmotive force โ not merely membrane potential. Bioblast 2022: BEC Inaugural Conference. <br>[[Komlodi 2022 MitoFit|ยป''MitoFit Preprint''ยซ]] ย | |title=[[File:KomlodiT.JPG|left|100px|Komlodi Timea]] <u>Komlรณdi Timea</u>, Tretter L (2022) The protonmotive force โ not merely membrane potential. Bioblast 2022: BEC Inaugural Conference. In: https://doi.org/10.26124/bec:2022-0001 <br>[[Komlodi 2022 MitoFit|ยป''MitoFit Preprint''ยซ]] ย | ||
|info=[https://wiki.oroboros.at/index.php/Bioblast_2022#Submitted_abstracts Bioblast 2022: BEC Inaugural Conference] | |info=[https://wiki.oroboros.at/index.php/Bioblast_2022#Submitted_abstracts Bioblast 2022: BEC Inaugural Conference] | ||
|authors= | |authors= |
Revision as of 09:37, 16 June 2022
Komlรณdi Timea, Tretter L (2022) The protonmotive force โ not merely membrane potential. Bioblast 2022: BEC Inaugural Conference. In: https://doi.org/10.26124/bec:2022-0001 ยปMitoFit Preprintยซ |
Link: Bioblast 2022: BEC Inaugural Conference
Komlodi Timea, Tretter Laszlo (2022)
Event: Bioblast 2022
The protonmotive force pmF establishes the link between electrical and chemical components of energy transformation and coupling in oxidative phosphorylation in the mitochondrial electron transfer system. The electrical part is corresponding to the mitochondrial membrane potential ฮฮจmt and the chemical part is related to the transmembrane pH gradient ฮpH. Although the contribution of ฮpH to pmF is smaller than that of ฮฮจmt, ฮpH plays an important role in mitochondrial transport processes and regulation of reactive oxygen species production. Separate measurement of ฮฮจmt and ฮpH allows for calculation of pmF. Methods for monitoring ฮฮจmt such as fluorescence dyes are generally available, while determination of ฮpH is more challenging.
In this review, we focus on the application of the fluorescence ratiometric method using the acetoxymethyl ester form of 2,7-biscarboxyethyl-5(6)-carboxyfluorescein (BCECF/AM) for real-time monitoring of the intramitochondrial pH in isolated mitochondria. Knowing the intra- and extramitochondrial pH allows for calculating the ฮpH. Application of specific ionophores such as nigericin or valinomycin, exerts the possibility to dissect the two components of the pmF in different directions. Furthermore, we tried to summarize those mitochondrial processes, such as production of reactive oxygen species, where the ฮpH has an important role.
โข Keywords: BCECF; intramitochondrial pH; matrix pH; ฮpH; mitochondria, mitochondrial membrane potential, ฮฮจmt; nigercin; protonmotive force, pmF; reverse electron transfer, RET; safranin; triphenylphosphonium, TPP+; valinomycin
โข O2k-Network Lab: HU Budapest Tretter L
Affiliations
- Department of Biochemistry, Semmelweis University, Budapest, Hungary
List of abbreviations, terms and definitions - MitoPedia
Labels: MiParea: Instruments;methods, mt-Membrane
Preparation: Isolated mitochondria
Enzyme: Complex V;ATP synthase
Regulation: ATP production, mt-Membrane potential, pH
Coupling state: LEAK, OXPHOS
HRR: Oxygraph-2k, O2k-Fluorometer, TPP