NADH electron transfer-pathway state: Difference between revisions
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[[NADH]]-linked substrates (CI-linked) | [[NADH]]-linked substrates (CI-linked) are [[substrate types |type N substrates]] of ETS-level 4, feeding electrons into the [[N-junction]] catalyzed by various mt-dehydrogenases. N-supported flux is induced in mt-preparations by addition of NADH-generating substrates individually or in combination: [[pyruvate]], [[glutamate]], [[malate]], [[oxoglutarate]], [[citrate]], [[hydroxybutyrate]]. These type N substrates are (indirectly) linked to [[Complex I]] by the corresponding dehydrogenase-catalyzed reactions reducing NAD<sup>+</sup> to NADH+H<sup>+</sup>. In mt-preparations, succinate dehydrogenase (SDH; [[CII]]) is largely substrate-limited in N-linked respiration, due to metabolite depletion into the incubation medium. The residual involvement of S-linked respiration in the pesence of type N substrates can be further suppressed by the CII-inhibitor [[malonic acid]]). | ||
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= List of publications: N-linked or CI-linked substrate state = | |||
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Revision as of 14:01, 23 March 2016
- high-resolution terminology - matching measurements at high-resolution
NADH electron transfer-pathway state
Description
NADH-linked substrates (CI-linked) are type N substrates of ETS-level 4, feeding electrons into the N-junction catalyzed by various mt-dehydrogenases. N-supported flux is induced in mt-preparations by addition of NADH-generating substrates individually or in combination: pyruvate, glutamate, malate, oxoglutarate, citrate, hydroxybutyrate. These type N substrates are (indirectly) linked to Complex I by the corresponding dehydrogenase-catalyzed reactions reducing NAD+ to NADH+H+. In mt-preparations, succinate dehydrogenase (SDH; CII) is largely substrate-limited in N-linked respiration, due to metabolite depletion into the incubation medium. The residual involvement of S-linked respiration in the pesence of type N substrates can be further suppressed by the CII-inhibitor malonic acid).
Abbreviation: N
Reference: N-junction
MitoPedia concepts:
Respiratory state
MitoPedia O2k and high-resolution respirometry: "SUIT state" is not in the list (O2k hardware, DatLab, Oroboros QM, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry) of allowed values for the "MitoPedia O2k and high-resolution respirometry" property. SUIT state"SUIT state" is not in the list (O2k hardware, DatLab, Oroboros QM, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry) of allowed values for the "MitoPedia O2k and high-resolution respirometry" property.
