Sobotka Ondrej

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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping

Sobotka Ondrej

MitoPedia topics: EAGLE

COST: Member COST WG1: WG1

COST WG3: WG3

Name	Sobotka Ondrej, MUDr., Ph.D.
Institution	Director of the MitoAnalytics Lab Oroboros Instruments Ondra joined Oroboros in January 2024. Ondrej Sobotka was a visiting scientist at the Medical University of Innsbruck from May 01 to August 31 2019 within the framework of Internalisation of research directions of University hospital in Hradec Kralove, Czech republic. Project registration number: CZ.02.2.69/0.0/0.0/16_027/0008513
Address	Schoepfstrasse 18, A-6020
City	Innsbruck
State/Province
Country	Austria
Email	[email protected]
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O2k-Network Lab	AT Innsbruck Oroboros, CZ Hradec Kralove Cervinkova Z

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Publications

	Published	Reference
Vernerova 2021 Biomedicines	2021	Vernerova A, Garcia-Souza LF, Soucek O, Kostal M, Rehacek V, Krcmova LK, Gnaiger E, Sobotka O (2021) Mitochondrial respiration of platelets: comparison of isolation methods. https://doi.org/10.3390/biomedicines9121859
Komlodi 2021 BEC AmR-O2	2021	Komlódi T, Sobotka O, Gnaiger E (2021) Facts and artefacts on the oxygen dependence of hydrogen peroxide production using Amplex UltraRed. Bioenerg Commun 2021.4. https://doi.org/10.26124/bec:2021-0004
MitoEAGLE blood cells 1	2020	Åsander Frostner Eleonor, Aburel Oana M, Avram Vlad F, Calabria Elisa, Castelo Rueda Maria Paulina, Chamkha Imen, Čižmárová Beata, Danila Maria-Daniela, Doerrier Carolina, Eckert Gunter P, Ehinger Johannes K, Elmer Eskil, Garcia-Souza Luiz F, Gnaiger Erich, Hoppel Florian, Karabatsiakis Alexander, Keppner Gloria, Kidere Dita, Krako Jakovljević Nina, Labieniec-Watala Magdalena, Lelcu Theia, Micankova Petra, Michalak Slawomir, Molina Anthony JA, Pavlovic Kasja, Pichler Irene, Piel Sarah, Rousar Tomas, Rybacka-Mossakowska Joanna, Schartner Melanie, Siewiera Karolina, Silaidos Carmina, Sjövall Fredrik, Sobotka Ondrej, Sumbalova Zuzana, Swiniuch Daria, Vernerova Andrea, Volani Chiara, Vujacic-Mirski Ksenija, Watala Cezary (2020) Interlaboratory guide to mitochondrial respiratory studies with peripheral blood mononuclear cells and platelets. - Updated: 2020-03-06 - *Confirmed
BEC 2020.1 doi10.26124bec2020-0001.v1	2020	Gnaiger E et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v1
Komlodi 2018 Methods Mol Biol	2018	Komlodi T, Sobotka O, Krumschnabel G, Bezuidenhout N, Hiller E, Doerrier C, Gnaiger E (2018) Comparison of mitochondrial incubation media for measurement of respiration and hydrogen peroxide production. Methods Mol Biol 1782:137-55.
Sobotka 2017 Thesis	2017	Sobotka O (2017) Pharmacological and metabolic influence on liver mitochondrial functions. PhD Thesis p127.
Sobotka 2016 J Bioenerg Biomembr	2016	Sobotka O, Endlicher R, Drahota Z, Kučera O, Rychtrmoc D, Raad M, Hakeem K, Červinková Z (2016) Impaired mitochondrial functions contribute to 3-bromopyruvate toxicity in primary rat and mouse hepatocytes. J Bioenerg Biomembr 48:363-73.
Mezera 2016 Oxid Med Cell Longev	2016	Mezera V, Endlicher R, Kucera O, Sobotka O, Drahota Z, Cervinkova Z (2016) Effects of epigallocatechin gallate on tert-butyl hydroperoxide-induced mitochondrial dysfunction in rat liver mitochondria and hepatocytes. Oxid Med Cell Longev 7573131:8pp.
Kucera 2016 Drug Chem Toxicol	2016	Kučera O, Endlicher R, Rychtrmoc D, Lotková H, Sobotka O, Červinková Z (2016) Acetaminophen toxicity in rat and mouse hepatocytes in vitro. Drug Chem Toxicol 40:448-56.
Sobotka 2015 Physiol Res	2015	Sobotka O, Drahota Z, Kučera O, Endlicher R, Rauchová H, Červinková Z (2015) The effect of α-tocopheryl succinate on succinate respiration in rat liver mitochondria. Physiol Res 64 Suppl 5:S609-15.

Abstracts

	Published	Reference
Sobotka 2024 EBEC	2024	Sobotka O, Cardoso L, Garcia-Souza L, Sobotka L, Gnaiger E (2024) The effect of glucose on mitochondrial respiration in hepatocellular carcinoma cells. EBEC 2024.
Sobotka 2022 Abstract Bioblast	2022	6.1. «10+5» Sobotka Lubos, Sobotka O (2022) The Crabtree effect and clinical nutrition. Bioblast 2022: BEC Inaugural Conference. In: https://doi.org/10.26124/bec:2022-0001
Sobotka 2022 Bioblast2022	2022	Bioblast 2022 participant.
Cardoso 2019 MiP2019	2019	Effects of glucose concentration in cell culture media on hepatocellular carcinoma HuH7 cells evaluated by high-resolution respirometry.
Sobotka 2018 MiP2018	2018	Measurement of ROS production under hypoxia and unexpected methodological pitfalls of Amplex UltraRed assay.
Gnaiger 2018 AussieMit	2018	Komlodi Timea, Sobotka Ondrej, Doerrier Carolina, Gnaiger Erich (2018) Mitochondrial H₂O₂ production is low under tissue normoxia but high at in-vitro air-level oxygen pressure - comparison of LEAK and OXPHOS states. AussieMit 2018 Melbourne AU.
Rodriguez-Hernandez 2018 Free Radic Biol Med	2018	Rodriguez-Hernández A, Staňková P, González R, De la Rosa AJ, Álamo-Martinez JM, Marin-Gómez LM, Sobotka O, Kučera O, Padillo FJ, Červinková Z, Muntané J (2018) The alteration of mitochondrial function is associated with the induction of endoplasmic reticulum stress autophagy and apoptosis by Sorafenib in liver cancer cells. Free Radic Biol Med.
Sobotka 2017 Abstract MITOEAGLE Barcelona	2017	The effect of 24 weeks of high-fat and high-cholesterol diet on rat liver mitochondria. Sobotka_Presentation
Sobotka 2017 MiPschool Obergurgl	2017	Reference protocols for ROS production measurements.
Nozickova 2017 MiPschool Obergurgl	2017	Sulfite oxidase.
Komlodi 2017 MiP2017	2017	H₂O₂ production under hypoxia in brain and heart mitochondria: does O₂ concentration matter?
Nozickova 2017 MiP2017	2017	The early adaptation of rat liver mitochondria to high fat diet.
Sobotka 2017 MiP2017	2017	Metabolic changes in rat liver mitochondria during the development of liver steatosis.
Gnaiger 2016 Abstract SFRR Australasia 2016	2016	Facts and artefacts in measurements of H₂O₂ production under hypoxia.
Sobotka 2016 Abstract IOC116	2016	Sobotka O, Kucera Otto, Stankova P, Endlicher R, Nozickova K, Banni A, Cervinkova Z (2016) Mitochondrial respiration in fatty liver. Mitochondr Physiol Network 21.11
Sobotka 2014 Abstract IOC95	2014	Sobotka O (2014) Effect of α-Tocopheryl succinate on Complex II. Mitochondr Physiol Network 19.10

MitoEAGLE Inclusiveness Target Countries - Conference Grant

- - - Conference Grant Grand Period 3

Scientific report - MiP2018

Participated at

Visiting scientist in the Oroboros O2k-Laboratory

Ondrej Sobotka : Visiting scientist at the Oroboros O2k-Laboratory

November 01 to December 23 2018 & May 01 to August 31 2019 within the framework of Internalisation of research directions of University hospital in Hradec Kralove, Czech republic. Project registration number: CZ.02.2.69/0.0/0.0/16_027/0008513
March 01 to April 25 2017 within the framework of the Postdoc Scholarship AKTION Czech Republic-Austria
March 01 to June 10 2016 within the framework of the Mobility fund of the Charles University