News and Events | Working Groups | Short-Term Scientific Missions | Management Committee | Members |
COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
MitoEAGLE protocols, terminology, documentation
WG1
- Standard operating procedures and user requirement document: Protocols, terminology, documentation
WG1 Description
- The quality management system, scope of SOPs to be elaborated and the outline of the data repositories will be defined in a user requirement document.
Respirometric reference protocols
- A ‘library of protocols’ applied in mitochondrial respiratory physiology will be collected in a standard format, delineating the diversity of experimental approaches to assess bioenergetic function. A set of reference protocols will rigorously document (Maelstrom Research program) the state-of-the-art standards in designing, conducting, reporting, interpreting, and validating such bioenergetic tests (see also Fig. 3).
Interlaboratory proficiency test
- Inter-laboratory ring tests are a basic requirement for quality management. Such proficiency tests require a reference sample which must be homogenous, stable, representative of the diagnostic target, amenable for distribution, and economical for large-scale production. For respiratory OXPHOS analysis a reference sample of structurally and functionally intact mitochondria is not yet available to perform corresponding ring tests (in contrast to enzymatic OXPHOS ring tests). Recently, the widely applied human cell line HEK 293T was found to be potentially suitable for cryopreservation. Upon thawing the cells can be used immediately for respirometry of both intact and permeabilized cells. Thus interlaboratory proficiency testing may now be feasible as a world-wide innovation in the field of mitochondrial respiratory physiology. Participating labs may implement the test for intra- and interlaboratory validation and longitudinal performance monitoring, using a reference OXPHOS protocol (Fig. 3). The organization of the proficiency test should follow the requirements of the ISO 17043 and 13528 standards. A corresponding MITOEAGLE proficiency training shall be developed and implemented. Feedback from the participants will lead to a final adjustment of the SOPs in the proficiency test, which will be made generally available.
Instrumental platforms
- Comparison of results between and within instrumental platforms: The reference protocols and proficiency test will not define the instrumental platform, but will allow a quantitative comparison of results obtained with specific equipment available in the participating laboratories.
Nomenclature
- Harmonization of nomenclature on mitochondrial respiratory states and control parameters: The logistics of development of a database requires application of strictly defined terms for all included variables. There is no general reference available upon which a consistent terminology on mitochondrial physiology and bioenergetics can be based. The MITOEAGLE consortium, therefore, has to accomplish the ambitious goal to unify and simplify the terminology in the field for the purpose of the QMS, which will lead to the development of recommendations for the use of a common terminology in mitochondrial physiological research. A publication will be prepared as an Open Access article which will be a milestone towards a unification of concepts and nomenclature.
Knowledge management system
- With an increase in data volume and complexity comes along the requirement for standardized report formats and digital accessibility of the data. Since such a database is still missing for mitochondrial physiology, the goal of WG1 is to establish an integrated and analytic environment linking mitochondrial respiration with external data (cell physiology, anthropometry, spiroergometry, lifestyle, genomics) in the context of physical fitness evaluation and clinical diagnostics. Databases will be established combining biological and clinical data comprising proprietary and publicly available datasets into one integrated solution. Data import and export functionalities will allow data upload and mapping to corresponding objects in the database, in this way generating valuable data and allowing for project-specific as well as cross-project data mining.
- The ability to formulate meta-studies in our approach is an important success factor: The platform can aggregate the project’s complete knowledge and provides the ability to make diagnostic profiling associated with diseases. In addition to functional data, other data types such as nutritional or environmental factors associated with disease prevention can be identified and aggregated into the MITOEAGLE Knowledge Management System.
- Querying the heterogeneous data types and result representation are the most important requirements of the knowledge platform besides to data integration and modelling. The application will feature different types of visualization techniques for all integrated data types. The database will be developed using the concept of multi-tier client-server architecture. Individual components are separated corresponding to their functionality. An upstream web server handles user requests, an application server performing business logic, and a back-end database system guaranties persistent data storage. All components are additionally separated by means of network security incorporating firewalls and are hosted in a secure environment. The platform administration ensures privacy regarding proprietary data by user rights policies.
- It will be the task of WG1 to continuously collect the input from all other WGs, compare, discuss and align SOPs as far as possible, and to finally develop recommendations for quality control, data reporting and data sharing beyond the published record. In addition, WG1 will develop concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation. Finally, it will provide a summary on strategic dissemination and an education programme for MITOEAGLE. The education programme will ensure that not only presently participating groups will adhere to the set standards but that also groups joining in at later points may smoothly fit into the community and help to further broaden and deepen our understanding on mitochondrial physiology in health and disease.
WG1 Participants
Country | City | Contact |
---|---|---|
Armenia | Yerevan | Trchounian K |
Australia | Melbourne | Saner Nicholas |
Australia | Syndey | Ton Riccardo |
Australia | Victoria | Winwood-Smith Hugh |
Australia | Melbourne | Hewakapuge Sudinna |
Australia | Melbourne | Lionett S |
Australia | Clayton Victoria | Alton Lesley |
Australia | Geelong, Victoria | McKenzie Matthew |
Australia | Melbourne | Cassar Samantha |
Australia | Melbourne | Stepto Nigel K |
Australia | Melbourne | Coughlan Melinda T |
Australia | Newtown, Sydney | Stocker Roland |
Australia | Sydney | Hoehn Kyle L |
Australia | Queensland | Radenkovic Filip |
Australia | Melbourne | Genders Amanda J |
Australia | Sydney | Krycer James R |
Australia | Clayton | Wolff Jonci Nikolai |
Australia | Queensland | Dong Lan-Feng |
Australia | Victoria | Van Bergen Nicole J |
Australia | Queensland | Fisher Joshua J |
Australia | Melbourne | Ferri Alessandra |
Australia | Queensland | Holland Olivia J |
Australia | Nedlands | Perks Kara L |
Australia | Melobourne | Botella Javier |
Australia | Southport | Vidimce Josif |
Australia | Melbourne | Kuang Jujiao |
Australia | Melbourne | Whitfield Jamie |
Australia | Burwood | Trewin Adam J |
Australia | Parkville | Hardee Justin P |
Australia | Melbourne, VIC | Muccini Anna Maria |
Australia | Nedlands | Filipovska Aleksandra |
Austria | Innsbruck | Cardoso Luiza HD |
Austria | Innsbruck | Perez Valencia Juan Alberto |
Austria | Innsbruck | Klocker Helmut |
Austria | Innsbruck | Gallee Leon |
Austria | Aldrans | Haider Markus |
Austria | Innsbruck | Schwarzer Christoph |
Austria | Innsbruck | Sobotka Ondrej |
Austria | Innsbruck | Karabatsiakis Alexander |
Austria | Innsbruck | Schmitt Sabine |
Austria | Klosterneuburg | Sazanov Leonid A |
Austria | Innsbruck | Nindl Veronika |
Austria | Innsbruck | Cecatto Cristiane |
Austria | Innsbruck | Garcia-Souza Luiz F |
Austria | Innsbruck | Laner Verena |
Austria | Innsbruck | Gnaiger Erich |
Austria | Innsbruck | Passrugger Manuela |
Austria | Innsbruck | Keller Markus A |
Austria | Salzburg | Kofler B |
Austria | Innsbruck | Meszaros Andras T |
Austria | Innsbruck | Thapa Maheshwor |
Austria | Vienna | Kozlov Andrey V |
Austria | Innsbruck | Plangger Mario |
Austria | Innsbruck | Schartner Melanie |
Austria | Innsbruck | Burtscher Martin |
Austria | Innsbruck | Goebel G |
Austria | Innsbruck | Di Marcello Marco |
Austria | Innsbruck | Bergmeister Lisa |
Austria | Innsbruck | Hoppel Florian |
Austria | Kapferer Werner | |
Austria | Innsbruck | Antunes Diana |
Austria | Innsbruck | Doerrier Carolina |
Belgium | Namur | Arnould Thierry |
Belgium | Leuven, Flanders | Baekelandt V |
Belgium | Brussels | Sonveaux P |
Belgium | Liege | Votion Dominique-Marie |
Belguim | Sart Tilman (Liège) | Kruse C |
Brasil | Porto Alegre | Haas Clarissa |
Brasilia | Limeira | Ropelle Eduardo R |
Brazil | Rio de Janeiro | Galina Antonio |
Brazil | Santa Maria - Rio Grande do Sul | Soares Felix Alexandre Antunes |
Brazil | São Paulo | Kowaltowski Alicia J |
Brazil | Sao Paulo | Silber Ariel Mariano |
Brazil | Jaboticabal | Oliveira Marcos Tulio |
Brazil | Rio de Janeiro | Oliveira Marcus F |
Brazil | São Paulo | Crispim Marcell |
Brazil | Rio de Janeiro | Vieyra Adalberto |
Brazil | Sao Paulo | Vercesi Anibal E |
Brazil | Santa Maria - RS | Goncalves Debora Farina |
Canada | Rimouski | Blier Pierre U |
Canada | Edmonton (Alberta) | Lemieux Helene |
Canada | Edmonton | Zaugg Michael |
Canada | Rimouski | Munro Daniel |
Canada | Antigonish | Weir M |
Canada | Edmonton | Lucchinetti Eliana |
Canada | Vancouver | Bovard Josh |
Canada | Hamilton | Tarnopolsky Mark A |
Canada | Guelph | Holloway Graham P |
Canada | Montréal | Breton Sophie |
Canada | Hamilton | Scott Graham R |
Canada | Ottawa | Darveau Charles-A |
Canada | Ottawa | Harper Mary-Ellen |
Canada | Montreal, Quebec | Bergdahl Andreas |
Canada | Vancouver, BC | Tausan Daniel |
Canada | Edmonton | Han Woo Hyun |
Canada | Winnipeg | Chowdhury Subir Roy |
Canada | Quebec | Joseph Vincent |
Canada | Saint John | Pulinilkunnil Thomas |
Canada | Charlottetown | Adiele Reginald C |
Canada | Moncton, NB | Pichaud Nicolas |
Chile | Coyhaique | Jana Prado Fabian |
China | Fuzhou, Fujian Province | Chen Qi |
China | Xi`an | Liu Jiankang |
China | Tianjin | Zhang Yong |
China | Pukou, Nanjing, | Gan Zhenji |
Croatia | Zagreb | Saric A |
Croatia | Zagreb | Curik I |
Croatia | Zagreb | Rubelj Ivica |
Croatia | Split | Ljubkovic M |
Czech Republic | Vestec | Rohlena Jakub |
Czech Republic | Vestec | Davidova Eliska |
Czech Republic | Prague 10 | Urban Tomas |
Czech Republic | Hradec Kralove | Stankova Pavla |
Czech Republic | Hradec Kralove | Cervinkova Zuzana |
Czech Republic | Pardubice | Rousar Tomas |
Czech Republic | Plzen -Severni predmesti | Jedlicka Jan |
Czech Republic | Prague | Drahota Zdenek |
Czech Republic | Pardubice | Majtnerova Pavlina |
Czech Republic | Prague | Krizova J |
Czech Republic | Ceske Budejovice | Zikova Alena |
Czech Republic | Prague | Houstek Josef |
Czech Republic | Hradec Kralove | Endlicher Rene |
Czech Republic | Pardubice | Handl Jiri |
Czech Republic | Praha 2 | Danhelovska Tereza |
Czech Republic | Hradec Kralove | Kucera Otto |
Czech Republic | Vestec | Ezrova Zuzana |
Czech Republic | Praha 2 | Zdrazilova Lucie |
Czech rep | Hradec Kralove | Falaye T |
Denmark | Copenhagen | Soendergaard SD |
Denmark | Copenhagen N | Maise Chroeis K |
Denmark | Aarhus N | Jespersen Nichlas Riise |
Denmark | Roskilde | Dalgaard Louise T |
Denmark | Copenhagen | Gonzalez-Franquesa Alba |
Denmark | Copenhagen | Kraunsoee R |
Denmark | Copenhagen | Dela Flemming |
Denmark | Hvidovre | Nehlin Jan O |
Denmark | Copenhagen N | Scheibye-Knudsen Morten |
Denmark | Aarhus N | Boetker Hans Erik |
Denmark | Copenhagen | Nielsen B |
Denmark | Copenhagen | Larsen Steen |
Denmark | Copenhagen N | Mortensen OH |
Egypt | Cairo | Abdel-Rahman Engy Ali |
Egypt | Cairo | Ali Sameh S |
Estonia | Tallinn | Shevchuk Igor |
Estonia | Tallinn | Truu Laura |
Estonia | Tallinn | Klepinina Lyudmila |
Estonia | Tallinn | Kaambre Tuuli |
Estonia | Tallinn | Vendelin Marko |
Estonia | Tartu | Paju Kalju |
Estonia | Tallinn | Tepp Kersti |
Estonia | Tallinn | Puurand Marju |
Estonia | Tallinn | Laasmaa Martin |
Estonia | Tallinn | Klepinin Aleksandr |
Estonia | Tallinn | Karro N |
Finland | Jyvaeskylae | Kainulainen Heikki |
Finland | Helsinki | Suomalainen Wartiovaara Anu |
Finland | Turku | Stier Antoine |
Finland | Helsinki | Jackson Christopher Benjamin |
France | Maisons-Alfort Cedex | Prola Alexandre |
France | Tours | Dumas Jean-Francois |
France | Nouzilly | Collin-Chenot Anne |
France | Plouzané | Salin Karine |
France | Paris | Bouillaud Frederic |
France | Lille | Montaigne David |
France | Angers | Spinazzi Marco |
France | Montpellier | Wrutniak-Cabello Chantal |
France | Martinique | Cano Sanchez Maria Consolacion |
France | Bordeaux | Amoedo Nivea Dias |
France | Bordeaux | Sarlak Saharnaz |
France | Gueguen Naig | |
France | Bordeaux cedex | Mourier A |
France | Bordeaux cedex | Rossignol Rodrigue |
France | Angers | Procaccio Vincent |
France | Paris | Armand Anne-Sophie |
France | Martinique | Neviere Remi |
Germany | Magdeburg | Schoenfeld Peter |
Germany | Marburg | Vogt Sebastian |
Germany | Wickert Anika | |
Germany | Giessen | Sommer Natascha |
Germany | Wilhelmshaven | Salmon Pablo |
Germany | Cologne | Wiesner Rudolf J |
Germany | Gießen | Silaidos Carmina |
Germany | Duesseldorf | Jelenik T |
Germany | Cologne | Trifunovic Aleksandra |
Germany | Marburg | Ramzan Rabia |
Germany | Freiburg | Schuele R |
Germany | Doermann Niklas | |
Germany | Frankfurt am Main | Viel Christian |
Germany | Duesseldorf | Haendeler Judith |
Germany | Cologne | Pesta Dominik |
Germany | Rostock | Sokolova Inna |
Germany | Mainz | Maull Felicia |
Germany | Duesseldorf | Piel Sarah |
Germany | Frankfurt | Osiewacz Heinz D |
Germany | Regensburg | Renner-Sattler Kathrin |
Germany | Neuherberg | Einer C |
Germany | Biberach an der Riß | Nold V |
Germany | Frankfurt | Warnsmann Verena |
Germany | Cologne | Ho Dieu Hien |
Germany | Giessen | Schulz Rainer |
Germany | Tuebingen | Kappler Lisa |
Germany | Ulm | Schaefer PM |
Germany | Jena | Szibor Marten |
Germany | Bremerhaven | Mark Felix Christopher |
Germany | Cologne | Maciej Sarah |
Germany | Hannover | Das Anibh Martin |
Germany | Düsseldorf | Goy C |
Germany | Mainz | Methner Axel |
Germany | Duesseldorf | Roden Michael |
Germany | Gießen | Grewal Rekha |
Germany | Munich | Zischka Hans |
Germany | Frankfurt | Hamann Andrea |
Greece | Thessaloniki | Lazou Antigone |
Greece | Athens | Trougakos Ioannis P |
Greece | Heraklion | Tavernarakis Nektarios |
Greece | Athens | Gumeni Sentiljana |
Greece | 15771 | Andreadou Ioanna |
Hungary | Budapest | Chinopoulos Christos |
Hungary | Szeged | Boros Mihaly |
Hungary | Budapest | Tretter Laszlo |
Hungary | Debrecen | Verebne Tar K |
Hungary | Budapest | Horvath G |
Hungary | Budapest | Komlodi Timea |
Hungary | Szeged | Ferdinandy Peter |
India | Varanasi | Sonkar Vijay K |
India | New Delhi | Thakkar Himani |
India | New Delhi | Vincent Vinnyfred |
India | Lucknow | Gayen Jiaur |
India | Kollam | Suravajhala Prashanth |
India | Haryana | Chakrabarti Sasanka |
India | Hyderabad | Jha Rajan Kumar |
Iran | Tehran | Safaei Zahra |
Iran | Tehran | Shirazi Reza |
Ireland | Dublin | O'Gorman Donal |
Ireland | Dublin | Porter Richard K |
Israel | Jerusalem | Saada Reisch Ann |
Israel | Rishon Le Zion | Hachmo Yafit |
Israel | Zrifin | Sova Marina |
Israel | Beer Sheva | Mishmar Dan |
Italia | Udine | Salvadego Desy |
Italien | Padova PD | Szabo Ildiko |
Italy | Catania | Messina Angela |
Italy | Padova | Bernardi Paolo |
Italy | St. Lorenzen | Harrison David K |
Italy | Bolzano | Zanon Alessandra |
Italy | Catania | De Pinto Vito |
Italy | Padova | Fernandez-Vizarra Erika |
Italy | Bologna | Gasparre G |
Italy | Udine | Grassi B |
Italy | Monserrato | Isola Raffaella |
Italy | Bolzano/Bozen | Volani Chiara |
Italy | Catania | Magri Andrea |
Italy | Bari | Attimonelli M |
Italy | Milan | Clementi Emilio |
Italy | Campobasso | Pallotta Maria Luigia |
Italy | Bologna | Genova Maria Luisa |
Italy | Rome | Scatena Roberto |
Italy | Ancona | Battino Maurizio |
Italy | Milano | Bottoni P |
Italy | Bologna | Paterlini S |
Italy | Bologna | Porcelli AM |
Italy | Padova | Viscomi Carlo |
Japan | Sapporo | Matsumoto J |
Japan | Itabashi-ku Tokyo | Tanaka Masashi |
Kitzbühel | Innsbruck | Fischer Michael J |
Korea | Seoul | Pak Youngmi Kim |
Korea | Incheon | Kwak Hyo Bum |
Korea (South) | Seoul | Lee Hong Kyu |
Latvia | Riga | Liepins Edgars |
Latvia | Riga | Volska Kristine |
Latvia | Riga | Inashkina I |
Latvia | Riga | Jansone Baiba |
Latvia | Riga | Dambrova Maija |
Latvia | Riga | Vilks Karlis |
Latvia | Riga | Svalbe Baiba |
Latvia | Riga | Zvejniece Liga |
Latvia | Riga | Stelfa Gundega |
Luxembourg | Strassen | Devaux Yvan |
Malaysia | Kuala Lumpur | Hassan Hazirah |
Malta | Msida | Vella Joanna |
Mexico | Tlalpan | Rodriguez-Enriquez Sara |
Mexico | Mexico City | Aparicio Trejo OE |
México | San Pedro Garza García, Nuevo Leon | Garcia-Rivas Gerardo |
Netherlands | GA Wageningen | Grefte Sander |
Netherlands | Maastricht | Grevendonk L |
New Zealand | Auckland | Hickey Anthony J |
New Zealand | Auckland | Ward Marie Louise |
New Zealand | Wellington | Berridge Michael V |
New Zealand | Auckland | Kaur Sarbjot |
Norway | Tromsoe | Boardman Neoma T |
Norway | Bergen | Tronstad Karl Johan |
Norway | Bergen | Blindheim Dan Filip |
Norway | Trondheim | Grill V |
Norway | Bergen | Dyrstad Sissel E |
Norway | Trondheim | Lerfall Joergen |
Norway | Bergen | Berge Rolf K |
Norway | Bergen | Roesland Gro Vatne |
Norway | Trondheim | Smenes Benedikte Therese |
Norway | Oslo | Eide L |
Norway | Bergen | Haavik Jan |
Norway | Bergen | Hoel Fredrik |
Norway | Trondheim | Rognmo O |
Norway | Bergen | Pettersen Nitschke Ina Katrine |
Poland | Lodz | Labieniec-Watala Magdalena |
Poland | Warsaw | Wieckowski Mariusz R |
Poland | Warsaw | Szewczyk Adam |
Poland | Warsaw | Kampa Rafal Pawel |
Poland | Warszawa | Jaskiewicz Anna |
Poland | Warsaw | Bednarczyk Piotr |
Poland | Krakow | Dembinska-Kiec Aldona |
Poland | Poznan | Jarmuszkiewicz Wieslawa |
Poland | Gdańsk | Kaczor Jan Jacek |
Poland | Warsaw | Zablocki Krzysztof |
Poland | Warsaw | Karkucinska-Wieckowska Agnieszka |
Poland | Lodz | Watala Cezary |
Poland | Warsaw | Sek Aleksandra |
Poland | Warsaw | Dymkowska Dorota |
Poland | Warsaw | Wasilewski M |
Portugal | Coimbra | Oliveira Paulo J |
Portugal | Coimbra | Grilo Luis |
Portugal | Coimbra | Minuzzi Luciele M |
Portugal | Lisboa | Macedo Maria Paula |
Portugal | Aveiro | Ferreira Rita Maria P |
Portugal | Porto | Oliveira Jorge |
Portugal | Porto | Oliveira Pedro Fontes |
Portugal | Cantanhede | Teodoro Joao Soeiro |
Portugal | Coimbra | Palmeira Carlos |
Portugal | Cantanhede | Rodrigues Ana Sofia |
Portugal | Porto | Crisostomo Luis |
Portugal | Porto | Moreira Bruno P |
Portugal | Coimbra | Santos Diana |
Portugal | Porto | Silva Ana Maria |
Portugal | Cantanhede | Amorim Ricardo |
Portugal | Porto | Beleza Jorge |
Portugal | Aveiro | Vitorino Rui Miguel Pinheiro |
Portugal | Coimbra | Dias Candida |
Portugal | Porto | Alves Marco G |
Portugal | Coimbra | Duarte Filipe Valente |
Portugal | Coimbra | Rolo Anabela Pinto |
Qatar | Doha | Guarch Meritxell Espino |
Republic of Ghana | Cape Coast | Tei BN |
Republic of Korea | Daejeon | Park SH |
Republic of Korea | Busan | Kim Hyoung Kyu |
Republic of Korea | Yuseong-gu Daejeon | Quang Don T |
Republic of Korea | Busan | Han Jin |
Republic of Serbia | Belgrade | Krako Jakovljevic Nina |
Republic of Serbia | Belgrade | Lalic Nebojsa M |
Republic of Serbia | Novi Sad | Andric Silvana |
Russia | Moscow | Pavlova Nadia |
Russia | Yoshkar-Ola | Dubinin M |
Russia | Moscow | Vinogradov Andrey D |
Serbia | Belgrade | Mandic M |
Serbia | Belgrade | Djordjevic M |
Serbia | Belgrade | Savkovic U |
Serbia | Belgrade | Misirkic Marjanovic Maja |
Singapore | Singapore | Singh Brijesh Kumar |
Singapore | Singapore | Hausenloy Derek J |
Slovakia | Bratislava 45 | Ferko Miroslav |
Slovakia | Košice | Cizmarova Beata |
Slovakia | Bratislava | Sumbalova Zuzana |
Spain | Sevilla | Bustos Matilde |
Spain | Barcelona | Garcia-Roves Pablo Miguel |
Spain | Madrid | Hernansanz-Agustin Pablo |
Spain | A Coruña | Mayan MD |
Spain | Barcelona | De la Torre Lara J |
Spain | Salamanca | Almeida Angeles |
Spain | Armilla (Granada) | Fernandez-Ortiz Marisol |
Spain | Las Palmas de Gran Canaria | Calbet Jose AL |
Spain | Madrid | Rial Eduardo |
Spain | Madrid | Aragones Lopez J |
Spain | Barcelona | Perales Jose Carles |
Spain | Barcelona | Goncalo Teixeira da Silva Rui |
Spain | Barcelona | Bravo-Sagua Roberto |
Spain | Barcelona | Zorzano Antonio |
Spain | A Coruña | Blanco FJ |
Spain | Granada | Hidalgo-Gutierrez A |
Spain | Madrid | Moran M |
Spain | Barcelona | Gil J |
Spain | Valencia | Casado Pinna Marta |
Spain | Barcelona | Gama Perez Pau |
Spain | Granada | Quiles JL |
Sweden | Stockholm | Nedergaard J |
Sweden | Lund | Ehinger Johannes K |
Sweden | Stockholm | Shabalina Irina G |
Sweden | Gothenburg | Bhuvanachandran NS |
Sweden | Stockholm | Larsen Filip J |
Sweden | Stockholm | Cardinale Daniele A |
Switzerland | Lausanne | Donnelly Chris |
Switzerland | Zurich | Gorr Thomas A |
Switzerland | Lausanne | Morato Fornaguera L |
Switzerland | Zürich | Zaugg Kathrin |
Switzerland | Basel | Bouitbir Jamal |
Switzerland | Lausanne | Weger M |
Switzerland | Lausanne | Zanou Nadege |
Switzerland | Lausanne | Amati Francesca |
Switzerland | Lausanne | Zalachoras I |
Switzerland | Lausanne | Canto Alvarez Carles |
Switzerland | Lausanne | Gebara E |
Switzerland | Lausanne | Sandi Carmen |
Switzerland | Lausanne | Burtscher Johannes |
Switzerland | Bern | Wyss RK |
Switzerland | Lausanne | Lagarrigue Sylviane |
Switzerland | Basel | Fornaro Mara |
Switzerland | Bern | Nuoffer Jean-Marc |
Switzerland | Lausanne | Place Nicolas |
Taiwan | Changhua City | Wei Yau-Huei |
The Netherlands | Maastricht | Nabben Miranda |
The Netherlands | BT Amsterdam | Wuest Rob CI |
The Netherlands | Delft | McMillan Duncan GG |
The Netherlands | Groningen | Bakker Barbara M |
The Netherlands | Maastricht | Schrauwen Patrick |
UK | London | Lane Nick |
UK | Canterbury, Kent | Gourlay Campbell W |
UK | London | Bettinazzi Stefano |
UK | London | Rodriguez Enrique |
UK | Cambridge | Brown Guy C |
USA | Cambridge | Wohlwend Martin |
USA | Los Angeles | Kwast Kurt E |
USA | Needham | Brown David A |
USA | Provo | Hancock Chad R |
USA | San Diego; La Jolla | Molina Anthony JA |
USA | Aurora | Ford Ellen |
USA | Boston | Kristal Bruce S |
USA | Ann Arbor | Tyrrell Daniel J |
USA | Corvallis | Newsom Sean A |
USA | St. Louis | Pietka Terri A |
USA | Columbus | Sims Carrie A |
USA | Philadelphia | Jang David H |
USA | Pittsburgh | Prochownik Edward V |
USA | Galveston | Durham William J |
USA | Davis | Adams Sean H |
USA | Miami | Fontanesi F |
USA | San Diego | Cannon Daniel T |
USA | Omaha | Kim Julian KS |
USA | Milwaukee | Dash Ranjan K |
USA | Torrance | Rossiter Harry B |
USA | Cleveland | Hoppel Charles L |
USA | Puskarich Michael A | |
USA | Bethesda | Sharma Pushpa |
USA | Cleveland | Tandler Bernard |
USA | Fort Collins | Chicco Adam J |
USA | Portland | Garlid Keith D |
USA | Rochester | Nair K Sreekumaran |
USA | Denton | Dzialowski Edward M |
USA | Orlando | Coen Paul M |
USA | Berkeley | Williams Caroline M |
USA | Orlando | Sparks Lauren M |
USA | Orlando | Goodpaster Bret H |
USA | Gainesville | Leeuwenburgh Christiaan |
USA | Denver | Van Hove Johan |
USA | Stanford | Stary Creed |
USA | Durham | Li Pingan Andy |
USA | La Jolla | Valentine Joseph Marco |
USA | Philadelphia | Towheed Atif |
USA | Norfolk | Lai Nicola |
USA | Corvallis | Robinson Matthew M |
USA | Seattle | Marcinek DJ |
USA | Aurora | Sparagna Genevieve C |
USA | Bethesda | Fessel Joshua Patrick |
USA | El Paso | Bajpeyi Sudip |
USA | Rochester | Lanza Ian R |
USA | Louisville | Skolik Robert A |
USA | Gainesville | Neyroud D |
USA | Fairbanks | Coker Robert H |
USA | Kansas City | Swerdlow Russell H |
USA | Fort Collins | Li Puma Lance C |
USA | Little Rock | MacMillan-Crow Lee Ann |
USA | Milwaukee | Kandel Sunil Mani |
USA | Colorado Springs | Jacobs RA |
USA | Orlando | Pino Maria F |
USA | Wakefield | Varricchio Frederick |
USA | Little Rock | Borsheim Elisabet |
USA | Aurora | Moreau Kerrie |
USA | Fayetteville | Iyer Shilpa |
USA | New Haven | Cavalcanti-de-Albuquerque Joao Paulo |
USA | Winston-Salem | Tomar Dhanendra |
USA | La Jolla | Schilling Jan M |
USA | East Lansing | Bazil Jason N |
USA | Chicago | Dayanidhi Sudarshan |
USA | Kansas City | Thyfault John P |
USA | Baton Rouge | Irving Brian A |
USA | Baton Rouge | Hand Steven C |
USA | Little Rock | Parajuli Nirmala |
USA | Winston-Salem | Gonzalez-Armenta Jenny L |
USA | Fayetteville | Rao RR |
USA | Corvallis | Batterson Philip M |
USA | Philadelphia | Falk Marni J |
USA | New York | Galkin Alexander |
USA | Nashville | Wasserman David H |
USA | Iowa City | Chaurasia Bhagirath |
USA | Indianapolis | Brozinick Joseph T |
USA | Fairbanks | O'Brien Kristin |
USA | San Diego | Patel Hemal H |
USA | Greenville | Neufer P Darrell |
USA | Atlanta | Jang Young Charles |
USA | Baltimore | Gonzalez-Freire Marta |
USA | Louisville | Menze Michael A |
USA | St. Louis | Abumrad Nada A |
USA | Iowa City | Wagner Brett A |
USA | Winston-Salem | Ahn Bumsoo |
... further results |
WG1 Management
Tasks
- Opening of the database to the research public for data search and moderated entry of new data sets.
- Development of interlaboratory proficiency ring test, of recommendation/certification reports and of a MITOEAGLE proficiency training module (with WG5).
- Sample distribution, ring test experiments (MITOEAGLE-proficiency training on two different points of time, 6 months apart), data analysis by experimenters and MITOEAGLE-proficiency test manager.
- Reports and publication: Reports with specific results in comparison with an anonymous data summary, and joint publication. Individual reports may be complemented by recommendations, and are summarized in an informal certification report (move to WG5).
- Instrumental platform comparison with reference sample applying comparable measurements.
- Draft, dissemination, collection and final formulation of a manuscript on concepts and terminology of mitochondrial physiology (with WG5).
- Collect and discuss procedures and experimental protocols for the evaluation of mitochondrial capacities and create a library of protocols: A database comprising reference SUIT protocols, standard experimental media, and detailed instructions for OXPHOS analysis is assembled.
- MITOEAGLE data management system (DMS) development: The DMS for further data preprocessing. The DMS will enable management and analysis of instrument-specific and project-specific data.
- MITOEAGLE database (DB) development: DB will facilitate high-level project specific analysis as well as meta-analyses across projects, visualization of project specific data as well as integrative analyses of datasets for various proprietary and/or publicly available datasets.
- Testing of developed software components regarding functionality and usability, modification and implementation. Deployment for routine testing in the labs of our strategic partners.
- Data input: Upload of datasets generated through WG 2-4 in internal lab developments, results of the proficiency ring test, and during application module developments. In addition, the database will be populated with available proprietary and public datasets.
- Develop recommendations for quality control, data reporting and data sharing beyond the published record. Develop concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation (move to WG5).
- Provide a summary on strategic dissemination and an education programme for MITOEAGLE (move to WG5).
Milestones
- Data sets entered in standardized format and database opened for the public
- Protocols for ring-tests and standard analysis and report scheme defined
- MITOEAGLE proficiency training workshop finished (move to WG5)
- Analysis is completed and joint publication submitted (with WG5)
- Publication of paper on concepts and terminology (with WG5)
- Library of protocols online
- Publication of recommended protocols and procedures
- Guidelines for acquisition, evaluation and data documentation available (with WG5)
- MITOEAGLE data management system finished
- MITOEAGLE database ready for use
- Software modifications incorporated following extensive tests
- Publication of MITOEAGLE-KMP
- Recommendations on data acquisition, evaluation and management published (with WG5)
- Concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation published (move to WG5)
Deliverables
- Guidelines for future research and recommendations for the evaluation of respiratory characteristics in human respirometric samples (with WG5)
- A database on mt fitness evaluated with human cells and tissues
- Training of researchers towards improved reproducibility of sample preparation and respirometric evaluation (move to WG5)
- Qualitative and quantitative evaluation of results obtained in ongoing studies by comparison with a reference sample
- An expanded MITOEAGLE database
- A joint publication documenting the results of the MITOEAGLE-PT (with WG5)
- A multi-authored publication towards a unification of concepts and nomenclature in mitochondrial physiology (with WG5)
- A library of protocols for sample preparation and examination by respirometry
- A multi-authored publication with recommendations of comparable standard protocols and procedures (with WG5)
- Guidelines as to the optimum use of a reference sample for determination of respiration and related parameters of interest
- The MITOEAGLE data management system
- A continuously updated MITOEAGLE database for the use as reference data and guideline towards application of optimized protocols, ultimately leading towards standardization.
- A joint publication on the use of the MITOEAGLE database and management system (with WG5)
- An education programme for the use of MITOEAGLE database and management system (move to WG5).
Projects in progress
- Interlaboratory proficiency ring test
- Testing of software components
- Concepts and terminology on mitochondrial physiology
- Procedures and experimental protocols for the evaluation of mitochondrial capacities, library of protocols
- Data management system (DMS)
- Database
Next steps
- Best practices for implementation of analytical and process standards.
References
- General requirements for the competence of testing and calibration laboratories » ISO/IEC 17025:2005