|News and Events||About COST Action MitoEAGLE||Working Groups||MiP/MitoEAGLE Traning School 2019 Coimbra PT||MitoEAGLE Task Group States and rates||Short-Term Scientific Missions||Inclusiveness Target Countries||Management Committee||Members|
- COST Action CA15203 MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
- COST Action CA15203 MitoEAGLE
- 1 Grant periods
- 2 STSM Grant Period 3
- 3 STSM Grant Period 2
- 4 STSM Grant Period 1
- 5 STSM Coordinators
- 6 COST criteria for evaluation of STSM applications
- 7 How to apply for a STSM
- 8 MitoEAGLE host institutions
|GP||GP time span||MC Meeting||Work and Budget Plan||STSM application deadline 1st Call||STSM application deadline 2nd Call||STSM time span||STSM final report deadline|
|2016-09-12||Brussels, BE 2016-09-12|
|1||2016-11-01 to 2017-04-30||Barcelona, ES 2017-03-21||WBP 1||2016-11-01||2016-11-20 to 2017-03-25||2017-04-25|
|2||2017-05-01 to 2018-04-30||Hradec Králové, CZ 2017-11-15||WBP 2||2017-05-13||2017-09-28|| 2017-06-01 to 2017-10-31
2017-11-01 to 2018-03-25
|3||2018-05-01 to 2019-04-30||Jurmala, LV 2018-09-18||WBP3.1/WBP3.2||-||2019-02-28||2018-05-15 to 2019-03-25||
|4||2019-05-01 to 2020-04-30||Belgrade, RS 2018-10-13||2019-05-01 to 2020-03-25||2020-04-25|
|5||2020-05-01 to 2020-09-11||2020-05-01 to 2020-08-01||2020-09-01|
STSM Grant Period 3
- Awardees 2018 May 15th to 2019 March 25th
- The final dates of the STSMs will have to be negotiated between the applicant and the host.
- 34,650.- EUR (Work and Budget Plan)
- 50% will be allocated to Inclusiveness Target Countries
- MC decision on 2017-11-18: STSM should be more focused to target specific goals in MoU, devoted to complete specific WG tasks towards a manuscript
- 1st Call: Deadline 2019-02-28
|Aasander Frostner Eleonor||Sweden||Gnaiger E (AT)||MitoEAGLE proficiency training||WG1 WG4|
|Avram Vlad Florian||Romania||Elmer E (SE)||MitoEAGLE WG4 manuscript||WG4|
|Calabria Elisa||Italy||Elmer E (SE)||WG4 Blood cells workshop and retreat: interlaboratory manuscript and data organization.||WG4|
|Calisto Filipa||Portugal||McMillan D (NL)||Proton translocation mechanism of Alternative Complex III||WG1|
|Gama Pérez Pau||Spain||Larsen S (DK)||WG1 and 2 human skeletal muscle workshop||WG2|
|Garcia-Souza Luiz F||Austria||Elmer E (SE)||Comparison of platelets isolation methods on respiration and activation markers||WG4|
|Karavaeva Iuliia||Denmark||Gnaiger E (AT)||Evaluation of mitochondrial respiration of brown adipocytes using non-canonical respiratory substrates for deorphanizing mitochondrial metabolite transporters.||WG3|
|Maseko Tumisang Edward||Czech Republic||Muntane J||Study of mitochondrial dysfunction induced by Sorafenib in primary hepatocytes and hepatocyte-derived cell line (HepG2), WG3 and WG4||WG3, WG4|
|Siewiera Karolina||Poland||Elmer E (SE)||WG 4 blood cell workshop and retreat||WG4|
|Silaidos Carmina||Germany||Elmer E (SE)||Working Group 4 blood cell workshop||WG4|
|Vilks Karlis||Latvia||Rustan AC (NO)||Examination of molecular mechanisms of acylcarnitine pathophysiology||WG1|
|Zdrazilova Lucie||Czech Republic||Gnaiger E (AT)||Mitochondrial respiration in different cell lines measured in the 2.0 mL and 0.5 mL chambers of the O2k-FluoRespirometer (Oroboros): an experimental basis for platform comparison with the Seahorse XFe24 Bioanalyzer (Agilent)||WG1|
|Vendelin Marko||Estonia||Schlattner U (FR)||Collaboration within MitoEAGLE network||WG1|
|Gonzalez-Franquesa Alba||Denmark||Garcia-Roves PM (ES)||White adipose tissue mitochondrial respiration protocols - Experiments and Results discussion||WG1, WG2|
|Pereira da Silva Grilo da Silva Filomena||Portugal||Gnaiger E (AT)||Unspecific effect of etomoxir on mitochondrial respiration||WG1, WG3|
|Giakoumaki Ifigeneia||United Kingdom||Wuest RC (NL)||Use of high-resolution respirometry to assess skeletal muscle mitochondrial respiration in humans after a 60-day bed rest||WG2|
STSM Grant Period 2
- Awardees 2017 June 01st to 2017 October 31st and 2017 Novemver 01st to 2018 March 25th
- The final dates of the STSMs will have to be negotiated between the applicant and the host.
- 35,000.- EUR (Work and Budget Plan)
- 4,800.- EUR (addition from rest budget of the MC-Meeting in Hradec Kralvoe)
- 1st Call: Deadline 2017-05-13
- 2nd Call: Deadline 2017-10-03
|Hungary||Gnaiger E (AT)||Simultaneous measurements of oxygen, mitochondrial membrane potential and NADH using the O2k-NextGen||WG1|
|Danila Maria-Daniela||Romania||Labieniec-Watala M (PL)||CA15203 MitoEAGLE||WG4|
|Dalmao Fernandez Andrea||Spain||Rustan AC (NO)||Fatty acid and glucose metabolism in cybrids from osteoarthritis subjects with focus on mitochondrial function and energy utilization.||WG1|
|Doerrier Velasco Carolina||Austria||Garcia-Roves PM (ES)||Inter-laboratory harmonization of protocols to set a final consensus for the evaluation of the technical skills needed for working with permeabilized skeletal muscle.||WG1, WG2|
|Duicu Oana Maria||Romania||Gnaiger E (AT)||MitoFit Coaching Days||WG4|
|Spain||Gnaiger E (AT)||Harmonization of respirometric protocols comparing isolated mitochondria and permeabilized muscle fibers||WG1, WG2, WG4|
|Giovarelli Matteo||Italy||Gnaiger E (AT)||Interlaboratory comparison of skeletal muscle fibers High Resolution Respirometry||WG2|
|Hidalgo-Gutierrez Agustin||Spain||Chakrabarti L (UK)||Mitochondrial proteomics in mouse models of CoQ deficiency: therapeutic approaches and aging - completed||WG1|
|Italy||Dubouchaud Herve (FR)||Mitochondrial oxidative capacity in heart mitochondria after selective AMPK deletion||WG2|
|Jaskiewicz Anna||Poland||De Palma C (IT)||Short-Term Scientific Missions MitoEAGLE||WG2|
|Lelcu Theia||Romania||Labieniec-Watala M (PL)||CA15203 MitoEAGLE||WG4|
|Poland||Gnaiger E (AT)||Training in the Oroboros MitoFit Laboratory – from SUIT protocols to interpretation and reporting||WG1, WG2, WG3, WG4|
|Ledo Ana Margarida||Portugal||Juhasz L (HU)||Respirometric Protocols for Intact Tissue Preparations - Regulation of mitochondrial respiration by bioactive gases.||WG1|
|Poland||Gnaiger E (AT)||MitoFit Coaching Days||WG4|
|Spain||Cervinkova Zuzana (CZ)||Regulation of the mitochondria respiration and ROS production during antitumoral Sorafenib-treatment in HepG2 cells||WG3|
|Poland||Gnaiger E (AT)||IOC 124 Schroecken AT||WG1, WG4|
|Austria||Lalic N (RS)||Quality control assessment of mitochondrial respiration- completed||WG1|
|Croatia||Armand AS (FR)||Role of mitochondria in satellite cell activation and differentiation modulated by the calcineurin/NFATc2 pathway: Implications of cellular bioenergetics, respiration and autophagy fluxes||WG1, WG2|
|Saura-Esteller Jose||Spain||Duchen MR (UK)||Study of mitochondrial form and function after fluorizoline treatment||WG2|
|Poland||Gnaiger E (AT)||Fluorecence LED2 Module - training in the Oroboros MitoFit Laboratory||WG4|
|Slovakia||Gnaiger E (AT)||Mitochondrial respiratory function of cryopreserved PBMC - completed||WG1, WG4|
STSM Grant Period 1
- Awardees 2016 November 20th and 2017 March 25th
|Dymkowska Dorota||Poland||Gnaiger E (AT)||Training in the MitoFit Laboratory - completed||WG1|
|Hungary||Gnaiger E (AT)||Standardization of protocols in mitochondria respirometry and fluorometry - completed||WG1|
|Hungary||Gnaiger E (AT)||Harmonizing protocols of high resolution respirometry and fluorometry - completed||WG1|
|Zanou Nadege||Switzerland||Garcia-Roves Pablo M (ES)||Role of Ryanodin receptor (RyR) 1 in skeletal muscle adaptations to exercise: physiology and metabolism - completed||WG1, WG2|
- 4 STSMs for 750.- EUR
- 3,000.- EUR (Work and Budget Plan)
- Notification of final decision by the Management Committee: 2016-11-12
- 2 STSMs for 750.- EUR
- 1,500.- EUR (addition from FSAC - open for further applications)
COST criteria for evaluation of STSM applications
Aims and scope of a STSM
- 1. Support individual mobility.
- 2. Specifically contribute to the scientific objectives of MitoEAGLE.
- 3. Strengthen the MitoAGLE network.
- 4. Foster collaborations by allowing researchers participating in the COST Action MitoEAGLE to visit an institution/organisation in
- 5. Learn new techniques.
- 6. Gain access to specific data, methods and instruments not available in the home institutions / organisations.
Researchers eligible for a STSM
- PhD students and Early Career Investigators (up to 8 years after the date of obtaining the PhD/doctorate, full-time equivalent) have priority.
- Senior scientists.
- We particularly encourage the application of researchers from “inclusiveness target countries” (Bosnia and Herzegovina, Bulgaria, Croatia, Cyprus, Czech Republic, Estonia, FYR Macedonia, Hungary, Latvia, Lithuania, Luxembourg, Malta, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Turkey).
- Gender balance will be monitored.
- Researchers are eligible to receive a STSM Grant if the country where they hold their primary affiliation is listed here: http://www.cost.eu/COST_Actions/ca/CA15203?parties.
- The home institution is the institution / organisation where the STSM applicant holds their primary affiliation as registered on their e-COST profile / STSM application / C.V. and where they are currently performing their main strand of research.
- The host institution concerns the institution / organisation that will host the successful applicant.
- STSM in which practical science is getting carried out.
- STSM which directly lead to tangible outcomes (e.g. writing papers/grants).
- Funding rule in section 7 of the COST Vademecum.
- The money will be returned AFTER the Grantee has completed her/his visit and submitted a report (a written report on the activities carried out during the STSM within 30 days of the end of the research visit)
- Researchers from Participating *Inclusiveness Target Country (ITC)can receive 50% of the grant upon completion of the 1st day of the STSM. The remaining 50% will be paid after the Grantee has completed her/his visit and submitted a report.
- Note: STSM grantees must make their own arrangements for all provisions related to personal security, health, social security and pension matters.
- Researchers from Participating Inclusiveness Target Country (ITC) can receive 50% of the grant upon completion of the 1st day of the STSM
- Early Career Investigators (PhD + 8 years)
- allows for:
- an extended time frame of between 91 days and 180 days
- 50-70% of the STSM budget will be devote 5to complete specific WG tasks towards a manuscript. Participants will be mainly invited to apply for STSM to join such workshops.
- 30-50% of the STSM budget will be still available for training activities.
How to apply for a STSM
- Interested researchers have to submit their application and supporting documents to the STSM Coordinator:Magdalena Labieniec-Watala
- Letter of invitation to the applicant from a senior researcher affiliated to the host institution.
- The submitted STSM application form (downloadable when the online application is submitted – see point 4 below).
- Motivation letter including an overview of the proposed activities that will be performed which must contain a plan of work for the visit highlighting the proposed contribution to the scientific objectives of MitoEAGLE.
- Letter of support from the home institution.
- Full C.V. (including a list of academic publications – if applicable).
- Read the funding rules in Section 7 of the COST Vademecum: »Guidelines
- Register for an e-COST profile at https://e-services.cost.eu/ - add your bank account details to your profile.
- Include in your application a letter of invitation from the host institution confirming that the host can undertake the STSM on the given dates. The final dates of the STSMs have to be negotiated between the applicant and the host.
- Complete, submit and download your STSM application online at: www.cost.eu/STSM.
- Send the submitted STSM application form and the supporting documents to the STSM Coordinator:
- Magdalena Labieniec-Watala: firstname.lastname@example.org
- The amounts granted for each individual STSM will be determined during the evaluation process by the STSM Coordinator, Chair and Co-chair.
- The selection of applicants is based on the scientific scope of the STSM application which must clearly compliment the overall objectives of MitoEAGLE outlined in the Memorandum of Understanding.
- Within 30 days from the end date of the STSM, the successful applicant must submit a scientific report to the host institution and to the STSM Coordinator. The Scientific Report must contain the following:
- purpose of the STSM
- description of the work carried out during the STSM
- description of the main results achieved
- future collaboration with the Host institution (if applicable)
- foreseen publications/articles resulting from the STSM (if applicable)
- confirmation by the Host institution of the successful execution of the STSM
- Failure to submit the scientific report within 30 days from the end date of the STSM will effectively cancel the Grant.
- Please note that the COST Association can request additional information to substantiate the information contained within the documents submittedby STSM applicant.
General: STSM duration and budget
- Five to 90 days.
- Early Career Investigators: up to 180 days.
- STSMs need to be carried out in their entirety within a single Grant Period
- The financial support is a contribution to the overall expenses and does not necessarily cover all expenses. Financial support is limited to cover travel, accommodation and meal expenses and is paid in the form of a Grant.
- Up to a maximum of EUR 160 per day for accommodation and meal expenses; up to a maximum of EUR 2,500.- per STSM.
- for ECIs, a maximum amount of EUR 3500 can be afforded to the Grantee for STSMs with a duration of between 91 and 180 days – For ECIs partaking STSMs with a duration of between 5 and 90 days, the limit of EUR 2 500 must be respected;
- Financial support is limited to cover travel, accommodation and meal expenses and is paid in the form of a Grant.
MitoEAGLE host institutions
- We would like to invite institutions who are willing to host MitoEAGLE Short Term Scientific Missions to join the list as an important information for potential applicants. Please, let us know the topics of expertise of your institution related to the MitoEAGLE Working Groups and Memorandum of Understanding, and the contact details of the responsible mentor.
- Host institutions have to be:
- from another COST country, or
- an approved near neighbour country (NNC) institution, or
- an approved international partner country (IPC) institution, or
- an approved EC / EU Agency / an approved European RTD Organisation or an approved International Organisation.
|Country||Host Institution||Mentors||Working Group||Techniques in the lab|
|AT||Medical University Innsbruck||Gnaiger E||WG1, WG2, WG3, WG4||High-resolution respirometry, O2k-Fluorometry; MitoEAGLE proficiency test, SUIT reference protocols; isolation of mitochondria from various tissues, intact and permeabilized cells, tissue homogenates, permeabilized fibres; mitochondrial physiology data repositories|
|AT||OROBOROS INSTRUMENTS||Doerrier Velasco CA, Drinnan M, Gnaiger E, Sumbalova Z||WG1, WG2, WG3, WG4||High-resolution respirometry, O2k-Fluorometry; MitoEAGLE proficiency test, SUIT reference protocols; isolation of mitochondria from various tissues, intact and permeabilized cells, tissue homogenates, permeabilized fibres; mitochondrial physiology data repositories|
|BE||University of Louvain (UCL) Medical School||Sonveaux P||WG1|
|CZ||Charles University||Cervinkova Z||WG1, WG3||Liver cells, Liver regeneration, Toxic liver injury in vivo and in vitro|
|DE||Technical University of Munich||Klingenspor M,Fromme T||WG1, WG3, WG4||Preparation of single cell suspensions / isolated mitochondria from cultured cells / murine tissues / human blood, respirometry (Oroboros Oxygraphs, Seahorse Flux Analyzer, legacy clark type electrodes), fluorescence based assays of membrane potential and superoxide production, wide array of molecular biological and biochemical methodology.|
|DE||University Hospital Regensburg||Renner-Sattler K, Kreutz M||WG1, WG4||Isolation and differentiation of human immune cells,(lymphocyte populations, monocytes, dendritic cells)metabolic analysis of human immune cells and cultured cells respirometry, PreSens technology, Seahorse Technology, measurement of metabolites as glucose and lactate by enzymatic assays, western blot analysis of mitochondrial proteins, determination of mitochondrial content, ROS production and membrane potential by FACS analysis;|
|DE||Institut für Vegetative Physiologie der Universität Köln||Wiesner RJ||WG1||Mouse genetics, physiology, proteomics, tissue function|
|DE||Heinrich Heine University||Ventura N||WG2, WG3|| We primarily use the nematode c.elegans for aging and toxicology study. We carry out: silencing of nuclear encoded mitochondrial genes, stress response assays, lifespan and neuromuscular assays
further details can be found on our webpage www.iuf-duesseldorf.com/ventura-lab.html
|EE||Tallinn University of Technology||Vendelin M, Birkedal R||WG1, WG2|| Experiments are mainly performed on isolated mouse cardiomyocytes (WT and KOs). We have been using earlier rat and trout cardiomyocytes as well. As a preparation we use either intact cells or saponin-permiabilized cells. From the techniques routinely used in the lab: oxygraphy (Strathkelvin with custom software); absorbance and fluorescence spectrophotometers; confocal [imaging and FCS/RICS] and fluorescence microscopy. Fluorescence microscopy is running on our custom software allowing us to combine fluorescence imaging with single cell mechanics (control over cell mechanical contraction via carbon fibers) and patch clamping (electrophysiology). [WG2]
In addition to wet lab, we have experience and are actively developing mathematical models of different aspects of heart function: bioenergetics, mechanical contraction, and electrophysiology [WG2]. Finally, all experiments are monitored via database developed in the lab. [WG1]
|EE||National Institute of Chemical Physics and Biophysics||Tepp K, Kaambre T||WG1, WG2, WG3||1) Oroboros 2k (4), one with fluorescence module. 2) Mitochondrial respirometry of permeabilized cells, fibers, and isolated mitochondria; Analysis of kinetic parameters of respiration and energy transfer in cell. 3) Analysis of energy transport pathways (creatine and adenylate kinase pathway) 4) Western blotting 5) Immunohistochemistry and imaging ( confocal microscopy).|
|EG||Helmy Institute of Medical Science||Ali SS, Abdel-Rahman EA||WG2, WG3, WG4||1. Metabolic Studies (two Oroboros O2k with fluorescence and ISE modules + Seahorse XF24 Flux Analyzer), 2. Reactive Oxygen Species detection, identification, and quantifications (Magnettech Benchtop electron paramagnetic resonance spectrometer with temperature and gas controllers + BMG multiplate reader for optical detections + WPI Free Radical Analyzers), Flowcytometric detections in suspended cells, 3. Cell Fractionation techniques (mitochondrial and synaptosomes isolations, isolations of platelets neutrophils from human blood, etc.), 4. Flow cytometry (Attune® Acoustic Focusing Flow Cytometer), 5. Molecular and Cell Biology techniques (Western Blot, RT-PCR, etc.), 6. Imaging (Fluoresence and confocal microscopy), 7. Membrane fluidity measurements by EPR spin labeling.|
|ES||INIBIC A Coruña||Mayan MD||WG1, WG3, WG4||Cell culture, molecular, cellular and electrophysiological thecniques. Human primary cells (chondrocytes, dermal fibroblast, keratinocytes), cell lines and animal models (mice).|
|ES||INIBIC-Hospital, Universitario A Coruña.||Blanco FJ||WG1, WG2, WG4||*Metabolic studies (Seahorse XFp8 Extracellular flux analyzer), * ROS production by flow cytometry, * NO production by Greise reactive, * Flow cytometry deteccion with several technicals to detect Apoptotic cells, Δψm, mitochondrial mass…; * ATP production; * Molecular and cell biology technique (PCR, RT-PCR, WB, …); * Imaging by fluorescence microscopy. * cell culture (primary cultures and cell lines)”|
|ES||University of Barcelona||Garcia-Roves PM, Perales JC||WG1, WG2, WG3, WG4|| High-resolution respirometry - different types of culture cells (normal and cancer)
- animal tissues (isolated and homogenized from mice): skeletal muscle, adipose tissue, liver, brain, heart
|ES||Universitat de Lleida||Boada J||WG1, WG2, WG3, WG4||Oxygraph 2k, GC-MS, LC-MS-Several SUIT protocols in intact and permeabilized cells and tissues|
|ES||Instituto de Biomedicina de Valencia (IBV-CSIC)||Casado Pinna M||WG1, WG3|
|ES||Vall D’Hebron Research Institute||Villena JA||WG3|
|ES||Universitat Autònoma de Barcelona||Quintana A||WG3||Cell-type specific translatome, trascriptome analyses, animal behavior, mitochondrial dynamics|
|ES||Instituto de Biomedicina de Sevilla||Muntane J||WG3, WG4|
|ES||Instituto Teofilo Hernando I+D del Medicamento||Cano-Abad MF||WG3||Neuronal cell culture, mitocondrial calcium measurements by Aequorin and fluorecents probes genetically encoded to the mitocondrial matrix pericam.|
|ES||Instituto de Investigaciones Biomédicas Alberto Sols (CSIC-UAM)||Eide L||WG3, WG4||We work mainly on the cardiovascular system (endothelium) and in the liver, human blood cells and tissues and on the regulation of mitochondrial function and its impact on disease|
|ES||Universitat of Granada||Acuna-Castroviejo D, Escames G, Lopez Garcia LC||WG2||Crude and purified mitochondrial preparations; ATP production determination; high resolution respirometry in isolated mitochondria and permeabilized fibers from miocardial and skeletal muscles; in vivo respirometry in zebrafish embryos; spectrophotometric measurement of cytochromes;sSpectrophotometric measurement of mitochondrial respiratory complexes activities; HPLC-EC and HPLC-UV determination of Coenzyme Q and adenosine nucleotides; RT-PCR (including mtDNA copy number), Western-Blot, histology and immunohistochemistry (including COX and SDH stains), oxidative stress markers (carbonyl groups, lipid peroxidation, glutathione redox state, activities of GPx, GRd and MnSOD, ...); mouse models of diseases including aging, sepsis, Parkinson's disease, mitochondrial diseases. Primary cell models of mitochondrial diseases; mouse phenotyping (locomotor activity, MRI and MRS).|
|ES||Severo Ochoa Molecular Biology Center||Cadenas S||WG2, WG4||Routine respiration, leak, oxphos, uncoupled respiration, non-mitochondrial respiration; Heart biopsies, cultured cells; Diseases models: Ischemia-reperfusion|
|FR||Université Paris-Descartes||Petit PX||WG1, WG2, WG4||(Oroboros Oxygraphs, Seahorse Flux Analyzer, legacy clark type electrodes)… TPP+ etc… - Image analysis (Opera, Amnis flow image cytometry and flow cytometry (cell shorters and cell analysers).Xcelligence systems for cel proliferation and toxicology, biochemistry and classical signal transduction|
|FR||University Paris-Descartes||Armand AS||WG1, WG2||Oroboros(Frederic Bouillaud)Seahorse (INEM)- Skeletal muscles, stem cells, nuclear factor NFATC2, cell biology and molecular biology, developmental biology, flow cytometry (analysis and sorting) and confocal|
|GR||National & Kapodistrian University of Athens||Trougakos IP||WG1, WG2, WG3, WG4|
|HU||Semmelweis University||Chinopoulos C||WG3, WG4||i) Mitochondrial respirometry in intact and permeabilized cells and isolated mitochondria; ii) fluorescence measurements of mitochondrial membrane potential in intact and permeabilized cells and isolated mitochondria; iii) assessment of mitochondrial substrate-level phosphorylation in intact and permeabilized cells and isolated mitochondria; iv) Western blotting; v) isolation of mitochondria; vi) cell culturing; vii) TR technique protocol for assessment of permeability transition in intact and permeabilized cells and isolated mitochondria; viii) assessment of ANT activity in permeabilized cells and isolated mitochondria; ix) determination of maximum calcium uptake capacity in permeabilized cells and isolated mitochondria.|
|HU||University of Debrecen||Bai P||WG2, WG3||Cellular models for white adipocytes, beige adipoctes, hepatocytes, skeltal muscle myoblasts/fibers, Oroboros oxygraph 2k for oxymetry on cells, Seahorse XF96 for oxymetry on cells, Flow cytometric determination of mitochondrial membrane potential, Assessment of mitochondrial fusion by microscopy, Gene expression studies, Assessment of the activity of certain cellular energy sensors (AMPK, mTORC1, mTORC2, etc.)|
|IL||Technion - Israel Institute of Technology||Ben-Shachar D||WG3|
|IL||University of Valencia||Borras C, Vina J||WG2, WG3, WG4||Oxidative stress-related parameters determination (HPLC, western blotting), Seahorse measurements (just starting with this), molecular biology (RT-PCR, ELISA…), mitochondrial isolation, cell cultures|
|IR||Trinity Biomedical Sciences Institute (TBSI)||Porter RK||WG1|
|IR||Dublin City University||O'Gorman D||WG2||Oroboros, Standard lab techniques (western blot, enzyme assay), Proteomics, Gene array, Microscopy (inverted, confocal, etc), Cell culture, Clinical facility (human data collection, including biopsies), Flow cytometry|
|IT||University of Bologna||Genova ML||WG2, WG4||Tissue fractionation and mitochondrial isolation techniques, First model OROBOROS High resolution Oxygraph and respirometric equipments of different brands, Gel electrophoresis apparatus for BN-PAGE, Spectrophotometric techniques for respiratory enzyme kinetic assays|
|IT||Fondazione S.Lucia -IRCCS||Pieroni L||WG2, WG3, WG4|| Mitochondria enrichment from tissues and cells ( with the possibility to culture cell lines), Wet Lab Biochemistry ( SDS-PAGE, Western Blot, IP, immunohistochemistry)
Proteomics Facilities: MALDI TOF MS/MS-MS: for PMF analysis on digested proteins or Biomarker Profiling in biofluids ( liquor, plasma, urines, etc..), LC-MS and LC-MS/MS: label free comparative proteomics, bottom up and top down analysis, targeted proteomics, GC-MS: metabolic survey analysis
|IT||University Hospital “Luigi Sacco”||De Palma C, Pirkmajer S||WG2||Mitochondrial respirometry in intact and permeabilized cells, isolated mitochondria and single muscle fibers; Western blotting; isolation of mitochondria from muscle and BAT; cell culturing (primary muscle stem cells and many different cell lines); real-time PCR (including mtDNA); muscle istology (including SDH and COX stainings and analysis of fiber type composition); measure of in vivo muscle performance; imaging (fluorescence and confoncal microscopy); flow cytometry; mouse models of muscle disease including Duchenne Muscular Dystrophy.|
|IT||University of Verona||Calabria E||WG4||Separation and isolation of PBMCs, HRR.|
|IT||Università Politecnica delle Marche||Battino M||WG3, WG4||Mitochondrial Respiration monitored by SeaHorse XF24|
|LT||Lithuanian University of Health Sciences||Borutaite V, Arandarcikaite O||WG2, WG3, WG4||Isolation of mitochondria from various tissues (brain, heart, liver, kidney), respirometry (isolated mitochondria and cells), measurement of mitochondrial permeability transition, activities of mitochondrial enzymes, cell culture techniques (primary neuronal-glial, cell lines - macrophages, glioblastoma and neuroblastoma)|
|LV||Latvian Institute of Organic Synthesis||Makrecka-Kuka M, Liepins E||WG1, WG2|| mt respirometry combined with fluorometry, enzyme activity measurements, radiolabeled substrate oxidation, molecular biology.
research of mitochondrial function after ischemia-reperfusion injury (ex vivo), in the models of oxidative stress and diabetes.
|NL||Academic Medical Center, Amsterdam||Wuest RC, Houtkooper RH||WG1, WG2, WG4||Mitochondrial respiration (Oroboros and Seahorse)Metabolomics, Cell culture, Metabolic disease models (large biobank with fibroblasts from patients), C. elegans, CRISPR/CAS9, Enzyme and DNA diagnostics, Cardiac cell function measurements, stable isotope flux measurements|
|NL||Delft University of Technology||McMillan D||WG1||Membrane preparation and membrane protein purification, proton-pumping assays (ATP synthase/cytochromes), lipid purification/handling, advanced reconstitution methods, tethered lipid bilayers, Biomimetic membrane technologies, Bioelectrochemistry for membrane-bound quinone activation and membrane analysis (impedance spectroscopy /cyclic volammetry/chronoamperometry), Membrane-protein/soluble protein interactions (QCM-D), quinone-lipid-protein interactions, micro-second time resolution freeze-quench and nano-spectroscopy (rapid pre-steady-state kinetics of proteins), single-molecule ATP synthase rotation, respirometry, flurorescence spectroscopy, mitochondrial isolation|
|NL||Wageningen University||Keijer J||WG2, WG3, WG4||Molecuar biology etc,Immunohistochemistry etc, Seahorse 96, Oroboros 2K, Indirect calorimetry, Ventilated hood (see: www.molecularphysiology.eu)|
|NO||University of Oslo||Rustan AC||WG2, WG4||Multiwell fuel handling system using radiolabeled precursors|
|NO||Institute of Microbiology||Eide L||WG1||mtDNA characterization: mutagenesis and mtDNA integrity assessment.Respiration analyses, mtDNA repair characterization|
|PL||University of Lodz||Labieniec-Watala M, Watala C||WG1, WG2, WG3, WG4||mt bioenergetics -mt-membrane potential with TPP electrode and fluorescence module (safranin) - Ca2+ level measurements (fluorescence module) - ATP production|
|PL||Poznan University of Medical Sciences||Michalak S||WG1, WG4||ELISA, Western blotting, indirect fluorescence, enzymology|
|PT||University of Coimbra||Palmeira C||WG1, WG2, WG3, WG4|
|RS||Faculty of Medicine University of Belgrade||Lalic N, Markovic I, |Krako Jakovljevic N||WG3, WG4||High-Resolution Respirometry (Oroboros Oxygraph 2k), Western Blot, Flow Cytometry, Transfection via Improved Electroporation (Lonza Nucleofector Technology)|
|SE||Stockholm University||Nedergaard J||WG1, WG3|
|SE||Lund University, Mitochondrial Medicine||Elmer E, Aasander Frostner E||WG4||OROBOROS 2k: intact cells protocols, permeabilized cells and tissues protocols, isolated mitochondria and homogenates. Titration-Injection microPump TIP2k and Fluorescence LED2 Module available.Seahorse XFe96: intact cells protocols (OCR, ECAR), Citrate synthase measurements, lactate production etc.|
|SI||University of Ljubljana||Mars T, Pirkmajer S||WG2, WG4||Human skeletal muscle cell cultures, skeletal muscle cell lines, co-cultures of human skeletal muscle cells and embryonic rat spinal cord (innervated cultured myotubes), human skeletal muscle biopsies, real-time PCR, Western blot, gene silencing (siRNA), ELISA, MAGPIX multiplex (Luminex), flow cytometry, and metabolic assays in cell culture.|
|SK||Slovak Academy of Sciences||Ukropec J, Ukropcova B||WG2, WG4||primary muscle cell culture, skeletal muscle & adipose tissue biopsy, O2k respirometry (muscle,.blood cells, muscle cell culture - could be established), real-time PCR, western blot (in-cell western), muscle IHCH, exercise intervention studies, clinical metabolic phenotyping, indirect calorimetry, human muscle functional characteristics, aerobic fitness test, physical activity monitoring|
|UK||University of Nottingham||Chakrabarti L||WG2, WG3||Proteomics, lipidomics with collaborators at UoN, enzyme biochemistry (electron transport chain and others), lifespan studies in c.elegans|
|UK||De Montfort University||Moisoi N||WG1, WG3, WG4|| Models: Cell culture and Drosophila models of Parkinson’s Disease
Techniques: High Resolution Respirometry, RT-qPCR, Western Blotting, Immunofluorescence (Microscopy), Measurements of Neurotransmiters by electrochemical detection (HPLC)
|UK||University of Cambridge||Murray AJ||WG1, WG2, WG3, WG4||HRR, enzyme activity assays, isolated heart perfusion, metabolomics, molecular biology|
|UK||University of Cambridge||Murray AJ||WG1, WG2, WG3, WG4||HRR, enzyme activity assays, isolated heart perfusion, metabolomics, molecular biology|
|UK||University College London||Duchen MR||WG4||Confocal imaging, fluorescence lifetime imaging and anisotropy measurements; NADH imaging and spectroscopy; EPR and ESR measurements (Prof Chris Kay); super-resolution imaging microscopy; 2photon imaging microscopy; IPScell technology; respirometry (Oroboros and Seahorse); FACS|