Difference between revisions of "MitoEAGLE data: blood and cultured cells"
From Bioblast
Line 11: | Line 11: | ||
:::: Biopsy sampling of human muscle and fat tissue for mitochondrial studies is an invasive approach. More recently measurement of mitochondrial function in human blood cells – as well as temporary cryopreservation (Karabatsiakis 2014 Transl Psychiatry) – has become an attractive and by far less invasive alternative for mitochondrial diagnostics. Mitochondrial function of blood cells was linked with numerous diseases and metabolic conditions such as sepsis, neurodegenerative diseases, depression, hypertension, amyotrophic lateral sclerosis, and early-stage heart failure (e.g. Sjoevall 2013 Crit Care; Karabatsiakis 2014 Transl Psychiatry; Hagl 2014 J Alzheimer Dis). The use of human blood cells will thus predictably widen the applicability of respirometry in human physiology and will provide a significant stimulus for the entire field of mitochondrial medicine. | :::: Biopsy sampling of human muscle and fat tissue for mitochondrial studies is an invasive approach. More recently measurement of mitochondrial function in human blood cells – as well as temporary cryopreservation (Karabatsiakis 2014 Transl Psychiatry) – has become an attractive and by far less invasive alternative for mitochondrial diagnostics. Mitochondrial function of blood cells was linked with numerous diseases and metabolic conditions such as sepsis, neurodegenerative diseases, depression, hypertension, amyotrophic lateral sclerosis, and early-stage heart failure (e.g. Sjoevall 2013 Crit Care; Karabatsiakis 2014 Transl Psychiatry; Hagl 2014 J Alzheimer Dis). The use of human blood cells will thus predictably widen the applicability of respirometry in human physiology and will provide a significant stimulus for the entire field of mitochondrial medicine. | ||
:::: A key towards achieving comparability among data sets and the applicability of human blood cells for the evaluation of mitochondrial fitness in health and disease is the standardization of the procedures to separate or isolate blood cells, the experimental procedure for the evaluation of mitochondrial respiratory characteristics, and the format for reporting. A consensus on protocols should be reached in WG4, calling for a Workshop right at the start of the COST Action. Depending on the WG4 participants input, cell lines will be defined for which harmonization of datasets should be achieved (Fig. 2). | :::: A key towards achieving comparability among data sets and the applicability of human blood cells for the evaluation of mitochondrial fitness in health and disease is the standardization of the procedures to separate or isolate blood cells, the experimental procedure for the evaluation of mitochondrial respiratory characteristics, and the format for reporting. A consensus on protocols should be reached in WG4, calling for a Workshop right at the start of the COST Action. Depending on the WG4 participants input, cell lines will be defined for which harmonization of datasets should be achieved (Fig. 2). | ||
== WG4 Participants == | |||
{{#ask:[[MitoPedia topic::EAGLE]] [[In country::+]] [[Has COST WG4::+]] | |||
| mainlabel=- | |||
|?In country=Country | |||
|?In city=City | |||
|?=Contact | |||
|sort=In country | |||
|order=ascending | |||
|limit=5000 | |||
|format=broadtable | |||
|offset=0 | |||
}} | |||
Revision as of 14:55, 27 April 2016
News and Events | Working Groups | Short-Term Scientific Missions | Management Committee | Members |
COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
MitoEAGLE data: blood and cultured cells
WG4
- MITOEAGLE data repository for blood cells and clutured cells
WG4 Description
- Biopsy sampling of human muscle and fat tissue for mitochondrial studies is an invasive approach. More recently measurement of mitochondrial function in human blood cells – as well as temporary cryopreservation (Karabatsiakis 2014 Transl Psychiatry) – has become an attractive and by far less invasive alternative for mitochondrial diagnostics. Mitochondrial function of blood cells was linked with numerous diseases and metabolic conditions such as sepsis, neurodegenerative diseases, depression, hypertension, amyotrophic lateral sclerosis, and early-stage heart failure (e.g. Sjoevall 2013 Crit Care; Karabatsiakis 2014 Transl Psychiatry; Hagl 2014 J Alzheimer Dis). The use of human blood cells will thus predictably widen the applicability of respirometry in human physiology and will provide a significant stimulus for the entire field of mitochondrial medicine.
- A key towards achieving comparability among data sets and the applicability of human blood cells for the evaluation of mitochondrial fitness in health and disease is the standardization of the procedures to separate or isolate blood cells, the experimental procedure for the evaluation of mitochondrial respiratory characteristics, and the format for reporting. A consensus on protocols should be reached in WG4, calling for a Workshop right at the start of the COST Action. Depending on the WG4 participants input, cell lines will be defined for which harmonization of datasets should be achieved (Fig. 2).
WG4 Participants
Country | City | Contact |
---|---|---|
Armenia | Yerevan | Trchounian K |
Austria | Innsbruck | Doerrier Carolina |
Austria | Innsbruck | Fischer Christine |
Austria | Innsbruck | Meszaros Andras T |
Austria | Innsbruck | Torres-Quesada Omar |
Austria | Innsbruck | Karabatsiakis Alexander |
Austria | Innsbruck | Hoppel Florian |
Austria | Innsbruck | Gnaiger Erich |
Austria | Innsbruck | Cardoso Luiza HD |
Austria | Innsbruck | Garcia-Souza Luiz F |
Belgium | Leuven | Bird Matthew |
Belgium | Namur | Renard P |
Belgium | Liege | Votion Dominique-Marie |
Belgium | Namur | Arnould Thierry |
Belgium | Namur | Detraux Damien |
Bosnia and Herzegovina | Tuzla | Jusic Amela |
Bosnia and Herzegovina | Tuzla | Hajrulahovic Anesa |
Brazil | Sao Paulo | Verdaguer Ignasi Bofill |
Brazil | Sao Paulo | Dominguez-Amorocho OA |
Brazil | Sao Paulo | Alencar Mayke Bezerra |
Brazil | São Paulo | Crispim Marcell |
Croatia | Zagreb | Rubelj Ivica |
Czech Republic | Prague | Fisar Zdenek |
Czech Republic | Pilsen | Kuncova J |
Czech Republic | Prague | Hansikova Hana |
Czech Republic | Prague | Pecina Petr |
Czech Republic | Prague 4 | Bajzikova Martina |
Czech Republic | Pilsen | Kripnerova M |
Czech Republic | Prague | Mracek Tomas |
Czech Republic | Vestec | Zobalova Renata |
Czech Republic | Prague | Elkalaf Moustafa |
Czech Republic | Hradec Kralove | Vernerova Andrea |
Czech Republic | Hradec Kralove | Maseko Tumisang Edward |
Czech Republic | Pilsen | Markova Michaela |
Czech Republic and Australia | Prague and Southport | Neuzil Jiri |
Czech rep | Hradec Kralove | Falaye T |
Denmark | Malov | Hey-Mogensen M |
Denmark | Copenhagen | Desler C |
Denmark | Copenhagen N | Mortensen OH |
Denmark | Copenhagen | Rasmussen LJ |
Denmark | Hvidovre | Nehlin Jan O |
Denmark | Aarhus | Rattan Suresh IS |
Egypt | Cairo | Abdel-Rahman Engy Ali |
Egypt | Cairo | Ali Sameh S |
Estonia | Tartu | Grellier T |
Estonia | Tallinn | Klepinina Lyudmila |
Estonia | Tallinn | Truu Laura |
Finland | Helsinki | Pirinen E |
Finland | Turku | Stier Antoine |
Finland | Jyvaeskylae | Lehti Maarit |
France | Bordeaux cedex | Mourier A |
France | Bron | Rizwan Alam M |
France | Paris | Petit Patrice X |
Germany | Freising | Schoettl T |
Germany | Neuherberg | Borchard Sabine |
Germany | Ulm | Kolassa Iris |
Germany | Biberach an der Riß | Nold V |
Germany | Ulm | Calzia Enrico |
Germany | Gießen | Silaidos Carmina |
Germany | Düsseldorf | Prigione Alessandro |
Germany | Duesseldorf | Eckers A |
Germany | Ulm | Schaefer PM |
Germany | Freising | Fromme Tobias |
Germany | Mainz | Vujacic-Mirski Ksenija |
Germany | Freising | Klingenspor Martin |
Germany | Leipzig | Ost Mario |
Germany | Mainz | Kalinovic S |
Germany | Regensburg | Renner-Sattler Kathrin |
Germany | Magdeburg | Vieira Ligo Teixeira Camila |
Germany | Duesseldorf | Piel Sarah |
Germany | Freising | Keppner Gloria |
Germany | Giessen | Eckert Gunter P |
Germany | Freising-Weihenstephan | Musiol Eva |
Germany | Ulm | Hartmann C |
Germany | Munich | Jastroch M |
Germany | Hamburg | Singer Dominique |
Greece | Athens | Trougakos Ioannis P |
Hungary | Budapest | Komlodi Timea |
Hungary | Debrecen | Verebne Tar K |
Hungary | Budapest | Tretter Laszlo |
Ireland | Dublin | Karavyraki Marilena |
Ireland | Dublin | Porter Richard K |
Ireland | Sligo | Murphy J |
Israel | Rishon Le Zion | Hachmo Yafit |
Israel | Jerusalem | Saada Reisch Ann |
Israel | Zrifin | Sova Marina |
Italy | Bari | Agrimi G |
Italy | Bologna | Porcelli AM |
Italy | Ancona | Battino Maurizio |
Italy | Milano | Bottoni P |
Italy | Bologna | Gasparre G |
Italy | Rome | Scatena Roberto |
Italy | Verona | Calabria Elisa |
Italy | Bologna | Genova Maria Luisa |
Italy | Bolzano | Pichler Irene |
Italy | Padova | Fernandez-Vizarra Erika |
Italy | Bolzano | Castelo Rueda Maria Paulina |
Italy | Bolzano/Bozen | Volani Chiara |
Italy | Bologna | Paterlini S |
Japan | Sapporo | Shirakawa R |
Latvia | Riga | Makarova Elina |
Latvia | Riga | Kidere Dita |
Malaysia | Kuala Lumpur | Abdul Karim Norwahidah |
Malta | Msida | Vella Joanna |
Netherlands | Nijmegen | Koopman Werner JH |
Netherlands | Groningen | Rots MG |
Netherlands | Wageningen | Keijer Jaap |
Norway | Oslo | Nikolic N |
Norway | Oslo | Rustan AC |
Norway | Bergen | Tronstad Karl Johan |
Norway | Oslo | Lund Jenny |
Norway | Oslo | Eide L |
Palestine | Ramallah | Stiban Johnny |
Poland | Lodz | Watala Cezary |
Poland | Poznań | Ludziejewska A |
Poland | Lodz | Labieniec-Watala Magdalena |
Poland | Poznan | Swiniuch Daria |
Poland | Lodz | Siewiera Karolina |
Poland | Warsaw | Kampa Rafal Pawel |
Poland | Warsaw | Sek Aleksandra |
Poland | Krakow | Kaczara P |
Poland | Poznan | Rybacka-Mossakowska Joanna |
Poland | Poznan | Michalak Slawomir |
Poland | Warsaw | Wasilewski M |
Poland | Warsaw | Dymkowska Dorota |
Portugal | Cantanhede | Pinho Sonia A |
Portugal | Coimbra | Palmeira Carlos |
Portugal | Catanhede | Pereira da Silva Grilo da Silva Filomena |
Portugal | Porto | Beleza Jorge |
Portugal | Porto | Carrageta D |
Portugal | Porto | Magalhaes Jose |
Portugal | Porto | Martins Ana Dias |
Portugal | Porto | Crisostomo Luis |
Portugal | Porto | Moreira Bruno P |
Portugal | Cantanhede | Bento Guida Jose Freitas |
Portugal | Porto | Oliveira Pedro Fontes |
Portugal | Coimbra | Carvalho Eugenia |
Portugal | Covilhã | Dias Tania R |
Portugal | Porto | Bernardino RL |
Portugal | Porto | Alves Marco G |
Portugal | Coimbra | Pereira Susana P |
Republic of Korea | Yuseong-gu Daejeon | Quang Don T |
Republic of Korea | Daejeon | Park SH |
Republic of Serbia | Belgrade | Pavlovic Kasja |
Republic of Serbia | Belgrade | Krako Jakovljevic Nina |
Romania | Timisoara | Muntean Danina M |
Romania | Timisoara | Betiu AM |
Romania | Timisoara | Lighezan R |
Romania | Timisoara | Avram Vlad F |
Romania | Timisoara | Danila Maria-Daniela |
Romania | Timisoara | Lelcu Theia |
Russia | Moscow | Itkis YS |
Russia | Moscow | Tsygankova P |
Russia | Moscow | Krylova T |
Saudi Arabia | Giza | Kotb IA |
Serbia | Belgrad | Markovic Ivanka |
Serbia | Belgrade | Misirlic-Dencic S |
Serbia | Belgrade | Isakovic A |
Serbia | Belgrade | Ivanovic Tijana |
Serbia | Belgrade | Jeremic M |
Serbia | Belgrade | Vukojevic M |
Slovakia | Bratislava | Ukropec J |
Slovakia | Bratislava | Sumbalova Zuzana |
Slovakia | Košice | Cizmarova Beata |
Slovakia | Bratislava | Ukropcova B |
Slovakia | Bratislava | Maderova D |
Slovakia | Bratislava | Nemec Michal |
Slovenia | Ljubljana | Pirkmajer Sergej |
Slovenia | Ljubljana | Mars T |
Slovenia | Ljubljana | Kopitar-Jerala Natasa |
Spain | Sevilla | Bustos Matilde |
Spain | Salamanca | Almeida Angeles |
Spain | Barcelona | Tura Ceide O |
Spain | Barcelona | Arago Marc |
Spain | Barcelona | Hyrossova Petra |
Spain | Barcelona | Gonzalez Casacuberta I |
Spain | Armilla (Granada) | Fernandez-Ortiz Marisol |
Spain | Barcelona | Smolders V |
Spain | Valencia | Casado Pinna Marta |
Spain | Barcelona | Zorzano Antonio |
Spain | Barcelona | Gil J |
Spain | Valencia | Victor Victor Manuel |
Spain | Granada | Quiles JL |
Spain | Barcelona | Juarez D |
Spain | Ourense | Reboredo-Rodriguez Patricia |
Spain | Armilla, Granada | Escames Germaine |
Spain | A Coruña | Mayan MD |
Spain | Lleida | Gonzalo Hugo |
Spain | Madrid | De los Rios C |
Spain | A Coruña | Blanco FJ |
Spain | Barcelona | Gama Perez Pau |
Spain | Valencia | Borras Consuelo |
Spain | Lleida | Nogueras L |
Spain | Barcelona | Dahdah Norma Ramon |
Spain | Madrid | Cano-Abad MF |
Spain | Madrid | Cadenas S |
Spain | Madrid | Monsalve Maria |
Spain | Valencia | Fuertes Agudo Marina |
Spain | Sevilla | Muntane Jordi |
Spain | Madrid | Lopez MG |
Sweden | Lund | Karlsson M |
Sweden | Lund | Westerlund E |
Sweden | Lund | Chamkha Imen |
Sweden | Lund | Elmer Eskil |
Sweden | Södertälje | Alfaro-Moreno E |
Sweden | Linköping | Fontes-Oliveira CC |
Sweden | Lund | Simon Serrano Sonia |
Sweden | Lund | Leffler M |
Sweden | Lund | Abliva |
Sweden, SE | Lund | Aasander Frostner Eleonor |
Switzerland | Lausanne | Zanou Nadege |
Switzerland | Geneva | Martinou JC |
The Netherlands | BT Amsterdam | Wuest Rob CI |
Turkey | Tekirdag | Aral Cenk |
Turkey | Ankara | Tuncay Erkan |
Turkey | Izmir | Aydin HH |
Turkey | Istanbul | Baykal AT |
Turkey | Ankara | Engin Ayse Basak |
USA | Philadelphia | Janowska Joanna |
USA | Fayetteville | Iyer Shilpa |
USA | Iowa City | Buettner Garry R |
USA | Fayetteville | Rao RR |
USA | St Louis | Welch R |
USA | Philadelphia | Starr Jonathan |
USA | Philadelphia | Kilbaugh Todd |
USA | Orlando | Coen Paul M |
USA | Baton Rouge | Irving Brian A |
United Kingdom | Birmingham | Thakker A |
United Kingdom | Brighton | Moore Anthony L |
United Kingdom | Birmingham | Joanisse S |
United Kingdom | Stoke-on-Trent | McStay Gavin P |
United Kingdom | Oxford | Makrecka-Kuka Marina |
United Kingdom | Leicester | Moisoi Nicoleta |
Uruguay | Montevideo | Radi R |
WG4 Management
Tasks
- Kick-off meeting to develop a working plan for the establishment of consensus protocols, reporting schemes, and the assignment of specific study tasks.
- Development of SOPs for cell separation.
- Development of laboratory protocols for individual blood cell types (and other cell types) for mitochondrial studies on intact and permeabilized cells.
- Application of SOPs in experimental studies during Short Term Scientific Missions and feeding of data into the MITOEAGLE data repository.
- Publication of SOPs and study results.
Milestones
- Consensus on protocols, reporting schemes and work assignments.
- Completed SOPs for cell preparation & laboratory protocols.
- Application study finished and data transmitted to MITOEAGLE data repository.
- Publication finished.
Deliverables
- SOPs for blood cell separation and respirometric characterisation open for the research community.
- MITOEAGLE data repository for comparative data evaluation, planning of future studies, data mining.
- Publication with a set of reference data.