MiPNet23.01 IOC126 Innsbruck AT: Difference between revisions
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|Hands-on continued: TPP+ chemical background experiment [[MiPNet14.05 TPP-mtMembranePotential]]. Β | |Hands-on continued: TPP+ chemical background experiment. [[MiPNet14.05 TPP-mtMembranePotential]]. Β | ||
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Revision as of 12:30, 23 January 2018
MiPNet23.01 IOC126 Innsbruck AT
Innsbruck AT, 2018 Jan 24-26. Oroboros advanced O2k-Workshop on mt-membrane potential: TPP+ and fluorescent dyes. IOC126. |
Oroboros (2018-01-24) Mitochondr Physiol Network
Abstract:
Oroboros advanced O2k-Workshop on mt-membrane potential: TPP+ and fluorescent dyes. Innsbruck, Austria; 2018 Jan 24-26.
β’ O2k-Network Lab: AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit
General information
- The Oroboros O2k-Workshop is an advanced course which covers an introduction to basic concepts on mitochondrial membrane potential: proton pumps, ion gradients, electrochemical potential (proton motive force) and the measurement of mt-membrane potential in biological samples (isolated mitochondria, homogenate) with potentiometric (O2k-TPP+ ISE-Module) and fluorescence method (TMRM, safranin/O2k-Fluo LED2-Module). Data analysis - from TPP+ to mt-membrane potential - constitutes a major topic.
Venue
- Information on travel and venue:IOC Innsbruck.
- Β» Hotel suggestion: Hotel Weisses Kreuz
- Mention "Oroboros" when booking in order to receive a discount.
Programme
Wednesday 24th Topic Room 09:00 Get-together: Introduction of participants and their research interests. MiPArt 09:30 Erich Gnaiger: Protonmotive force and respiratory control. MitoEAGLE preprint 2017-09-21. MiPArt 10:00 DatLab 7, DL-Protocols. MiPArt 10:30 Coffee/Tea 11:00 Hands-on: Assembly and maintenance of TPP+ and reference electrodes. MiPNet15.03 O2k-MultiSensor-ISE. MitoFit ORO Lab 12:15 Lunch break 13:30 Determination of mt-membrane potential with potentiometric O2k-TPP+ ISE-Module - an introduction. MiPArt 14:30 Hands-on: Set up of the instruments with TPP+ and reference electrodes, air calibration and calibration of TPP+ electrode. MitoFit ORO Lab 16:30 Coffee/Tea 17:00 Hands-on continued: TPP+ chemical background experiment. MiPNet14.05 TPP-mtMembranePotential. MitoFit ORO Lab
Thursday 25th Topic Room 09:00 Hands-on: Air calibration and calibration of TPP+ electrode. MitoFit ORO Lab 09:30 Introduction in planning and performing a TPP+ experiment. MitoFit ORO Lab 10:30 Coffee/Tea 11:00 Hands-on continued: TPP+ experiment with mouse brain mitochondria. MitoFit ORO Lab 12:30 Lunch break 14:00 From the TPP+ signal to mt-membrane potential - guide through the Excel templates. TPP-Matematics-MembranePotential. MitoFit ORO Lab 14:30 Analysis of TPP+ experiment. Interdependence between O2 flux and mt-membrane potential. MitoFit ORO Lab 16:00 Coffee/Tea 16:30 The four compartment model - the effect of parameters on calculated mt-membrane potential. The analysis of the model. MiPArt 17:30 Erich Gnaiger: Protonmotive force and chemiosmotic pressure: a generalization of non-ohmic flux control of the proton leak. Abstract. MiPArt
Friday 26th Topic Room 09:00 Determination of mt-membrane potential with fluorescence method (TMRM, safranin) - O2k FluoRespirometer - an introduction. MiPNet22.11 O2k-FluoRespirometer manual, MiPNet20.13 Safranin mt-membranepotential. MiPArt 10:00 Hands-on: Air Calibration and O2k setup for fluorescence experiment, choosing fluorescence sensors. MitoFit ORO Lab 10:30 Coffee/Tea 11:00 Hands-on: Calibration of safranin/TMRM, experiment with mouse brain mitochondria. MitoFit ORO Lab 12:30 Lunch break 14:00 Analysis of TMRM/safranin experiment, comparison of TPP+ and fluorescence results. MitoFit ORO Lab 15:30 Coffee/Tea 16:00 Comparison of TPP+ and fluorescence methods for evaluation of mt-membrane potential. Examples in different protocols. MiPArt 17:00 Interdependence between O2 flux and mt-membrane potential. MiPArt 17:30 The Bioblast wiki, O2k-Network and MitoEAGLE, feedback and conclusions. MiPArt 18:00 Farewell activity MiPArt
Tutors and Lecturers
Tutors
Sumbalova Zuzana, PhD, Medical University of Innsbruck - Principal investigator
Chang Shao-Chiang MSc., Oroboros Instruments, Medical University of Innsbruck - PhD. student TRACT
Passrugger Manuela, BSc., Oroboros Instruments - Biomedical Assistant
Ana Carolina Bastos Sant'Anna Silva, MSc., Oroboros Instruments, Medical University of Innsbruck - PhD. student TRANSMIT
Lecturers and assistance
Doerrier Carolina,PhD, Oroboros Instruments - Chief Scientific Officer
Komlodi Timea,Dr., Oroboros Instruments - Scientific Researcher
Meszaros Andras, MD, PhD, Oroboros Instruments - Postdoctoral researcher
List of participants
Participant | Institution | |
---|---|---|
Duicu Oana-Maria | RO Timisoara Muntean DM - University of Medicine and Pharmacy | |
de Kok MichΓ©le | NL Leiden Lindeman JH - Leiden University Medical Center | |
Fernandez-Vizarra Erika | UK Cambridge Zeviani M - University of Cambridge | |
Garcia e Souza Luiz Felipe | AT Innsbruck Oroboros - Leopold-Franzens-University | |
Hunger Miriam | AT Innsbruck Oroboros | |
Janssen Connie | NL Leiden Lindeman JH - Leiden University Medical Center | |
Labieniec-Watala Magdalena | PL Lodz Watala C - University of Lodz | |
Michalak Slawomir | PL Poznan Michalak S - Poznan University of Medical Sciences | |
Siewiera Karolina | PL Lodz Watala C - University of Lodz | |
Smirnova Irina | SE Stockholm Smirnova I - Stockholm University | |
Steffen Anja | NL Leiden Lindeman JH - Leiden University Medical Center |
Registration
- closed
Contact
- Oroboros Instruments
- High-Resolution FluoRespirometry
- SchΓΆpfstrasse 18
- A-6020 Innsbruck, Austria
- Tel: +43 512 566796
- Fax: +43 512 566796 20
- Mitochondria and cell research
IOC recommended reading
Project support
Labels:
ORO, IOC, MitoFit, Next, 2018
O2k-Workshops and MitoFit Coaching Days are listed as MitoGlobal Events.