Wolf 2019 EMBO J: Difference between revisions
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|abstract=The mitochondrial membrane potential (ฮฮจm ) is the main driver of oxidative phosphorylation (OXPHOS). The inner mitochondrial membrane (IMM), consisting of cristae and inner boundary membranes (IBM), is considered to carry a uniform ฮฮจm . However, sequestration of OXPHOS components in cristae membranes necessitates a re-examination of the equipotential representation of the IMM. We developed an approach to monitor ฮฮจm at the resolution of individual cristae. We found that the IMM was divided into segments with distinct ฮฮจm , corresponding to cristae and IBM. ฮฮจm was higher at cristae compared to IBM. Treatment with oligomycin increased, whereas FCCP decreased, ฮฮจm heterogeneity along the IMM. Impairment of cristae structure through deletion of MICOS-complex components or Opa1 diminished this intramitochondrial heterogeneity of ฮฮจm . Lastly, we determined that different cristae within the individual mitochondrion can have disparate membrane potentials and that interventions causing acute depolarization may affect some cristae while sparing others. Altogether, our data support a new model in which cristae within the same mitochondrion behave as independent bioenergetic units, preventing the failure of specific cristae from spreading dysfunction to the rest. | |abstract=The mitochondrial membrane potential (ฮฮจm ) is the main driver of oxidative phosphorylation (OXPHOS). The inner mitochondrial membrane (IMM), consisting of cristae and inner boundary membranes (IBM), is considered to carry a uniform ฮฮจm . However, sequestration of OXPHOS components in cristae membranes necessitates a re-examination of the equipotential representation of the IMM. We developed an approach to monitor ฮฮจm at the resolution of individual cristae. We found that the IMM was divided into segments with distinct ฮฮจm , corresponding to cristae and IBM. ฮฮจm was higher at cristae compared to IBM. Treatment with oligomycin increased, whereas FCCP decreased, ฮฮจm heterogeneity along the IMM. Impairment of cristae structure through deletion of MICOS-complex components or Opa1 diminished this intramitochondrial heterogeneity of ฮฮจm . Lastly, we determined that different cristae within the individual mitochondrion can have disparate membrane potentials and that interventions causing acute depolarization may affect some cristae while sparing others. Altogether, our data support a new model in which cristae within the same mitochondrion behave as independent bioenergetic units, preventing the failure of specific cristae from spreading dysfunction to the rest. | ||
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== Cited by == | |||
{{Template:Cited by Komlodi 2022 MitoFit pmF}} | |||
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Latest revision as of 10:20, 3 April 2022
Wolf DM, Segawa M, Kondadi AK, Anand R, Bailey ST, Reichert AS, van der Bliek AM, Shackelford DB, Liesa M, Shirihai OS (2019) Individual cristae within the same mitochondrion display different membrane potentials and are functionally independent. EMBO J 38:e101056. |
Wolf DM, Segawa M, Kondadi AK, Anand R, Bailey ST, Reichert AS, van der Bliek AM, Shackelford DB, Liesa M, Shirihai OS (2019) EMBO J
Abstract: The mitochondrial membrane potential (ฮฮจm ) is the main driver of oxidative phosphorylation (OXPHOS). The inner mitochondrial membrane (IMM), consisting of cristae and inner boundary membranes (IBM), is considered to carry a uniform ฮฮจm . However, sequestration of OXPHOS components in cristae membranes necessitates a re-examination of the equipotential representation of the IMM. We developed an approach to monitor ฮฮจm at the resolution of individual cristae. We found that the IMM was divided into segments with distinct ฮฮจm , corresponding to cristae and IBM. ฮฮจm was higher at cristae compared to IBM. Treatment with oligomycin increased, whereas FCCP decreased, ฮฮจm heterogeneity along the IMM. Impairment of cristae structure through deletion of MICOS-complex components or Opa1 diminished this intramitochondrial heterogeneity of ฮฮจm . Lastly, we determined that different cristae within the individual mitochondrion can have disparate membrane potentials and that interventions causing acute depolarization may affect some cristae while sparing others. Altogether, our data support a new model in which cristae within the same mitochondrion behave as independent bioenergetic units, preventing the failure of specific cristae from spreading dysfunction to the rest.
Cited by
- Komlรณdi et al (2022) The protonmotive force - not merely membrane potential. MitoFit Preprints 2022 (in prep)
- Komlรณdi et al (2022) The protonmotive force - not merely membrane potential. MitoFit Preprints 2022 (in prep)
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